scholarly journals Flavonoids of Enhydra Fluctuans Exhibit Anticancer Activity against Ehrlich's Ascites Carcinoma in Mice

2010 ◽  
Vol 5 (8) ◽  
pp. 1934578X1000500
Author(s):  
Santanu Sannigrahi ◽  
Upal Kanti Mazumder ◽  
Arijit Mondal ◽  
Dilipkumar Pal ◽  
Silpi Lipsa Mishra ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Life Span ◽  
Anticancer Activity ◽  
Antioxidant Enzyme ◽  
Hematological Parameters ◽  
Antioxidant Enzyme Activity ◽  
Percentage Increase ◽  
Ehrlich's Ascites Carcinoma ◽  
Ehrlich’S Ascites Carcinoma

Flavonoids obtained from Enhydra fluctuans (FEF) were screened for anticancer activity against Ehrlich's ascites carcinoma (EAC) bearing Swiss albino mice. The anticancer activity was assessed by measuring the tumor growth response, percentage increase of life span, hematological parameters, lipid peroxidation, and antioxidant enzyme activity, like GSH and CAT. Two flavonoids, baicalein 7- O-glucoside and baicalein 7- O-diglucoside, were isolated from the ethyl acetate fraction. Treatment with FEF caused a significant decrease in the tumor cell volume and increase of life span. All the hematological parameters, malonaldehyde content and antioxidant enzyme activity were restored towards the normal level. FEF was found to be cytotoxic in the in-vitro model.

Heat stress and antioxidant enzyme activity in bubaline (Bubalus bubalis) oocytes during in vitro maturation

2016 ◽  
Vol 60 (9) ◽  
pp. 1357-1366 ◽  
Author(s):  
Syma Ashraf Waiz ◽  
Mohammad Raies-ul-Haq ◽  
Suman Dhanda ◽  
Anil Kumar ◽  
T. Sridhar Goud ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Heat Stress ◽  
Antioxidant Enzyme ◽  
In Vitro Maturation ◽  
Bubalus Bubalis ◽  
Antioxidant Enzyme Activity

scholarly journals ANTI PROLIFERATIVE ACTIVITY OF CALAMUS ROTANG AS A SPOTLIGHT ON EHRLICH’S ASCITES CARCINOMA TREATED PERITONEAL AS WELL AS SOLID TUMOR MODEL

2018 ◽  
Vol 10 (1) ◽  
pp. 85
Author(s):  
Subeer Roy ◽  
Diksha Kumari ◽  
Mainak Chakraborty ◽  
Pallab Kanti Haldar
Keyword(s):  
Life Span ◽  
Cell Count ◽  
Anticancer Activity ◽  
Solid Tumor ◽  
Tumor Volume ◽  
Hematological Parameters ◽  
In Vitro Cytotoxicity ◽  
Control Group

Objective: Methanol extract of Calamus rotang (MECR) root was appraised as a spotlight for the candidate of anticancer activity through the vehicle (Ehrlich Ascites Carcinoma) on Swiss albino mice.Methods: In vitro cytotoxicity assay has been accessed by trypan blue and MTT assay. In vivo anticancer activity was done using EAC cells (2 × 106) where in each groups mice were 6. After treatment with MECR at the lower dose of 200 and higher dose of 400 mg/kg respectively for 9 d, half of the mice of each group were sacrificed and the rest were kept to check prolongation of life span. The anticancer potential of MECR was evaluated by tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters, biochemical estimations and Furthermore, tissue antioxidant parameters. Besides, solid tumor activity was also inspected.Results: In MECR treated groups (200 and 400 mg/kg) tumor volume, packed cell volume and viable cell count was significantly lessened as compared to that of the EAC control group. Life span, most reliable criteria for anticancer study, increased quite surprisingly by 50% and 100% in a dose dependant manner while compared to EAC control group. The hematological, biochemical and liver tissue antioxidant parameter are significantly (p<0.05) restored along with solid tumor case study (solid tumor volume) towards the normal level after treatment with MECR.Conclusion: From the above study it can be inferred that the MECR has impressive anticancer activity in dose dependent way.

scholarly journals Antioxidant Properties of White Sesame (Sesamum indicum L.) Flour on Human Liver Cells In Vitro

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 39
Author(s):  
Maria Eugenia Sabatino ◽  
Agustín Lucini Mas ◽  
Martin Gustavo Theumer ◽  
Marcela Martinez ◽  
Daniel Wundelin ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Antioxidant Properties ◽  
Sesamum Indicum ◽  
Antioxidant Enzyme Activity ◽  
Beneficial Effects ◽  
Potential Source ◽  
Oxidative Effects ◽  
Gpx Activity

White sesame (Sesamum indicum L.) flour, an edible residue resulting from seed oil extraction, may become a novel alternative of healthy products due to its high polyphenols content—compounds with many beneficial effects for human health. In the present study, the antioxidant properties of sesame flour were characterized in human cells in vitro. The hepatic cell line HepG2 was treated for 24 h with degreased sesame flour extracts or pinoresinol—one of the main polyphenols of this flour. Then oxidative stress was induced by H2O2 exposure. Cell viability and reactive oxygen species amount were measured by flow cytometry. Antioxidant enzyme activity of glutathione peroxidase (GPx), reductase (GR) and catalase (CAT) were determined by spectrophotometry. Results showed that pinoresinol decreased H2O2 oxidative effects by up to a 40%, increasing CAT and GR activity without compromising cellular viability. The largest dose of sesame flour extracts decreased H2O2 stress induction by up to 46%, but increased cell death levels. Additionally, sesame flour raised CAT and GPx activity by 100%. These findings suggest that sesame flour has antioxidant properties through antioxidant enzyme activity modulation. Since pinoresinol is one of the major polyphenols of sesame flour, this industrial residue might be a potential source for functional foods with health benefits.

Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model

2012 ◽  
Vol 90 (8) ◽  
pp. 1095-1103 ◽  
Author(s):  
Martine Kienzle Hagen ◽  
Ana Ludke ◽  
Alex Sander Araujo ◽  
Roberta Hack Mendes ◽  
Tânia Gatelli Fernandes ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Disease Model ◽  
Fold Increase ◽  
Antioxidant Enzyme Activity ◽  
Nitrate Content ◽  
Isolated Soy Protein ◽  
Nitrite Nitrate

This study analyzed and compared the content of isoflavones in 2 soy products, the effectiveness of isoflavones as antioxidants, in vitro, and demonstrated the antioxidant effect of a soy diet in rats with myocardial infarction (MI). Isoflavone content was analyzed in soybean hypocotyl (SH) and isolated soy protein (ISP). The quality (TAR) and quantity (TRAP) of antioxidants present in the samples was quantified. The amount of daidzin was higher in SH (9 times) and genistein in ISP (5 times). SH presented a 3-fold increase in TAR, while both products exhibited same TRAP. The rats were fed an ISP diet for 9 weeks. Animals were distributed among 6 treatment groups: (i) Sham Casein; (ii) Infarct Casein < 25%; (iii) Infarct Casein > 25%; (iv) Sham Soy; (v) Infarct Soy < 25%; and (vi) Infarct Soy > 25%. MI was induced 5 weeks after the commencement of the diets. Lipid peroxidation (LPO), antioxidant enzyme activity, and levels of nitrites/nitrates were determined in blood. Rats receiving the ISP diet demonstrated increased activity of antioxidant enzyme activity and nitrite/nitrate content. In addition, the increase in LPO seen in rats subjected to MI was significantly mitigated when the ISP diet was given. These findings suggest a nutritional approach of using a soy-based diet for the prevention of oxidative-stress-related diseases such as heart failure.

scholarly journals Anti-tumor potential of cucurbitacin triterpenoids of Momordica dioica Roxb. fruit by EAC induced ascites tumor model

2020 ◽  
Vol 11 (2) ◽  
pp. 1793-1797
Author(s):  
Madesh T ◽  
Abhinav Raj Ghosh ◽  
Krishna K L ◽  
Seema Mehdi ◽  
Nandini H S ◽  
...  
Keyword(s):  
Body Weight ◽  
Life Span ◽  
Hematological Parameters ◽  
Tumor Model ◽  
In Vivo Studies ◽  
Percentage Increase ◽  
Mice Model ◽  
Anticancer Efficacy

Momordica dioicaRoxb. (Cucurbitaceae) is commonly known as spiny gourd and traditionally used as astringent, febrifuge, antiseptic, anthelmintic, spermicidal and also used in bleeding piles, urinary infection and as a sedative. Studies indicate that it possesses antioxidant, hepatoprotective, antibacterial, anti-inflammatory, anti-lipid peroxidative, hypoglycaemic and analgesic properties. In this study, the anticancer efficacy of Cucurbitacins obtained from Momordica dioicaRoxb. (MDR) has been evaluated. Based on previous in-vitro studies performed, in-vivo studies were carried out on mice model.  Ehrlich ascites carcinoma (EAC) cells were inoculated into swiss albino mice intraperitoneally to form a liquid tumor and then treated with oral administration of 50, 100, 200mg/kg. Evaluation parameters involved the mean survival time (MST), body weight, hematological parameters, Percentage increase in life span were measured in normal control, EAC control and Cucurbitacintreated groups (n = 6). Treatment with Cucurbitacins enriched fraction has shown anti-tumor effects against liquid tumor as indicated by a significant (P < 0.05) reduction in body weight. Interestingly, the enriched bio fraction restored the altered hematological parameters of tumor-bearing animals and significantly increased their life span. These data indicate the cytotoxic potential effects of MDRon tumor cells opening new opportunities for further studies on the anti-cancer effects of this agent.

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