Ionomic, metabolic and hormonal characterization of the phenological phases of different tomato genotypes using omics tools

ABSTRACT The correct characterization of germplasm banks is fundamental for breeders to succeed in breeding programs. Several studies have sought to obtain genotypes with resistance to pests. However, there is no consensus about which methodology is the most appropriate to characterize a germplasm bank of tomato with different levels of resistance to pests. The objective of this study was to compare methods of multivariate analysis for the evaluation of genetic diversity in tomato genotypes with different levels of resistance to pests. The experiments were conducted at the Vegetable Experimental Station of the Federal University of Uberlândia - Monte Carmelo campus (18º 42’ 43.19” South latitude and 47º 29’ 55.8” West longitude, 873 m altitude), in the period from April 2013 to November 2016. Sixteen genotypes were evaluated from the interspecific cross between LA-716 (S. pennellii) versus pre-commercial line (UFU-057) followed by backcrossing and self-fertilization, along with the pre-commercial line UFU-057 (recurrent parent) Santa Clara and the wild accession S. pennellii (donor genitor). The contents of acylsugar, foliar trichomes, South American tomato pinworm and leaf miner repellency tests were analyzed. The experimental design was the randomized block design totaling 76 plots (19 genotypes x 4 blocks). It was concluded that there was genetic variability among the evaluated genotypes. The method of graphic dispersion by principal components revealed a greater power of discrimination. Genotypes UFU-057F2RC27#4.3, UFU-057F2RC28#2.2 and UFU-057F2RC27#4.7 contain the highest levels of acylsugar, resistance to Liriomyza spp. and T. absoluta.

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Morphoagronomic Characterization of Tomato Plants and Fruit: A Multivariate Approach

Advances in Agriculture ◽

10.1155/2015/572321 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Aniela Pilar Campos de Melo ◽

Paulo Marçal Fernandes ◽

Fábio Venturoli ◽

Carlos de Melo Silva-Neto ◽

Aurélio Rubio Neto

Keyword(s):

Descriptive Analysis ◽

Principal Component ◽

Productive Capacity ◽

Tomato Plants ◽

External Appearance ◽

High Productivity ◽

New Material ◽

Multivariate Cluster ◽

Tomato Genotypes

Consumers in the fresh fruit market choose fruits mainly following criteria related to the external appearance. However, the introduction of new material for planting depends on the productive capacity of the plant as well as on the formation of fruit that meets consumer desires. Given the above, the objective of this study was to morphoagronomically characterize tomato genotypes using multivariate statistics. The genotype seedlings (Ellus, Black Mauri, Green Zebra, Green Tomato, Pomodoro Marmande, Pomodoro Fiorentino, Pitanga, and Black Krim) were transplanted 30 days after sowing. The morphoagronomic characterization of the genotypes was carried out by evaluating plants and fruits. The data were analyzed using descriptive analysis, namely, position and variability measurements. In addition, a multivariate cluster analysis and a principal component analysis were carried out for plant and fruit attributes. The cluster and principal component analyses were efficient in characterizing plants and/or fruits of different tomato genotypes. Such efficiency enhances result interpretation and proposed inferences, with applied relevance for the producers. The genotype Ellus has a combination of morphoagronomic plant and fruit traits superior to other genotypes. Such superior traits enable a high productivity.

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Towards a unified characterization of phenological phases: Fluctuations and correlations with temperature

Physica A Statistical Mechanics and its Applications ◽

10.1016/j.physa.2010.10.043 ◽

2011 ◽

Vol 390 (4) ◽

pp. 680-688 ◽

Cited By ~ 10

Author(s):

Diego Rybski ◽

Anne Holsten ◽

Jürgen P. Kropp

Keyword(s):

Phenological Phases

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Morphological and Molecular Characterization of Some Tomato (Solanum lycopersicum L.) Genotypes Collected From Erzincan Province of Turkey

10.21203/rs.3.rs-1243010/v1 ◽

2022 ◽

Author(s):

HALIL IBRAHIM OZTURK

Keyword(s):

Genetic Diversity ◽

Fruit Weight ◽

Solid Content ◽

Soluble Solid Content ◽

Solanum Lycopersicum L ◽

Soluble Solid ◽

Polymorphism Rate ◽

Tomato Genotypes ◽

Local Farmers

Abstract BackroundTurkey is a country with different geographical features and therefore it is extremely diverse in plant diversity. Tomato is one of the most important vegetables produced both in the world and in Turkey. In this study, it was aimed to determine the genetic diversity of 24 tomato genotypes collected from local farmers from “Center villages” and “Üzümlü” district in Erzincan province.Methods and ResultsMorphological (qualitative and quantitative) and molecular markers (ISSR) were used to determine genetic diversity among genotypes. Genotype 24 was found to be higher than other genotypes with important quantitative morphological features such as fruit length, fruit width, fruit weight and soluble solid content (SSC). Considering the overall morphological traits, a wide variation was detected between genotypes. According to the molecular findings obtained. The polymorphism rate ranged from 0–100% and the average polymorphism rate was calculated as 80%.ConclusionPresent findings revealed the diversity in tomato genotypes collected from Erzincan province and may constitute the bases for further breeding studies in tomato and will bring an integrity in tomato identification studies.

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THERMAL REQUIREMENT AND PHENOLOGICAL CHARACTERIZATION OF PAPAYA VARIETIES CULTIVATED UNDER SUBTROPICAL CLIMATE

Irriga ◽

10.15809/irriga.2019v24n2p367-377 ◽

2019 ◽

Vol 24 (2) ◽

pp. 367-377

Author(s):

Marcelo De Souza Silva ◽

Sarita Leonel ◽

Jackson Mirellys Azevêdo Souza ◽

Rafael Bibiano Ferreira ◽

Joyce Helena Modesto ◽

...

Keyword(s):

Carica Papaya ◽

Growth Curves ◽

Plant Performance ◽

Subtropical Climate ◽

Degree Days ◽

Thermal Requirement ◽

Thermal Sum ◽

Time Required ◽

Phenological Phases

THERMAL REQUIREMENT AND PHENOLOGICAL CHARACTERIZATION OF PAPAYA VARIETIES CULTIVATED UNDER SUBTROPICAL CLIMATE MARCELO DE SOUZA SILVA1; SARITA LEONEL2; JACKSON MIRELLYS AZEVÊDO SOUZA2; RAFAEL BIBIANO FERREIRA2; JOYCE HELENA MODESTO2 E ANA CAROLINA BATISTA BOLFARINI2 1 Departamento de Produção Vegetal, Faculdade de Agronomia e Engenharia Florestal – FAEF, Rod. Cmte. João Ribeiro de Barros, Km 420, 17400-000, Garça - SP, Brasil, [email protected]. 2 1Departament. de Agronomia/Horticultura, Faculdade de Ciências Agronômicas da UNESP, Av. Universitária, nº 3780 - Altos do Paraíso, 18610-03, Botucatu – SP, Brasil, [email protected], [email protected], [email protected], [email protected], [email protected]. 1 ABSTRACT The thermal sum has been widely used to correlate phenological phases of a culture, a technique used to quantify the time required for any phenological phase in any type of environmental conditions, independently of the species. In view of the above, this study aimed to evaluate the thermal requirement and phenological characterization of papaya varieties cultivated under subtropical climate. It was measured the following phenological stages length: from planting to flowering (PF), from flowering to harvest (FH) and from planting to the beginning of harvest (PH), by measuring the time elapsed between each phenological phase. Female and hermaphrodite flowers were considered for measuring the growth curves of fruits by evaluating length (LF) and diameter (DF) of the fruits at 15-day intervals. The thermal requirement was measured by the accumulation of thermal sum in degree-days (DD) during different phenological phases and fruits development period. In current study conditions, it was possible to infer that Sunrise Solo variety and hybrid Tainung nº 1 are earlier, with an approximately 10-month interval between planting and the beginning of the harvest; therefore, necessitating accumulation of 1722.2 and 1798.1 degree-days, respectively. Keywords: Carica papaya L., degree-days, plant performance. SILVA, M. S.; LEONEL, S.; SOUZA, J. M. A.; FERREIRA, R. B.; MODESTO, J. H.; BOLFARINI, A. C. B. EXIGÊNCIA TÉRMICA E CARACTERIZAÇÃO FENOLÓGICA DE VARIEDADES DE MAMOEIRO CULTIVADAS SOB CLIMA SUBTROPICAL 2 RESUMO A soma térmica tem sido amplamente utilizada para correlacionar fases fenológicas de uma cultura, uma técnica utilizada para quantificar o tempo necessário para qualquer fase fenológica em qualquer tipo de condição ambiental, independentemente da espécie. Frente ao exposto, objetivou-se com este estudo avaliar a exigência térmica e caracterização fenológica de variedades de mamoeiro cultivadas sob clima subtropical. Foi mensurado a duração dos seguintes estádios fenológicos: plantio ao florescimento (PF), florescimento à colheita e plantio ao início da colheita, através da aferição do tempo decorrido entre cada fase fenológica. As curvas de crescimento dos frutos, acompanhadas em flores femininas e hermafroditas, foram determinadas mediante avaliação do comprimento e diâmetro dos frutos em intervalos de 15 dias. Já a exigência térmica foi mensurada através do acumulo da soma térmica em graus-dia, durante as diferentes fases fenológicas e para o período de desenvolvimento dos frutos. Nas condições do presente estudo foi possível inferir que a variedade Sunrise Solo e o híbrido Tainung nº1 são mais precoces, com intervalo entre o plantio e o início da colheita de aproximadamente 10 meses, necessitando do acúmulo de 1722,2 e 1798,1 graus-dia, respectivamente. Palavras-chave: Carica papaya L., graus-dia, performance de plantas.

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Characterization of tomato accessions for resistance to early blight

Cropp Breeding and Applied Biotechnology ◽

10.1590/s1984-70332011000200010 ◽

2011 ◽

Vol 11 (2) ◽

pp. 174-180 ◽

Cited By ~ 12

Author(s):

José Fernando Jurca Grigolli ◽

Mirian Maristela Kubota ◽

Daniel Pedrosa Alves ◽

Gabriel Belfort Rodrigues ◽

Carine Rezende Cardoso ◽

...

Keyword(s):

Early Blight ◽

Block Design ◽

Research Field ◽

Diseased Leaf ◽

Breeding Programs ◽

Progress Curve ◽

Randomized Block Design ◽

Diseased Leaf Area ◽

Tomato Genotypes

The purpose of this study was to characterize 50 tomato genotypes of the Vegetable Genebank of the Federal University of Viçosa. They were evaluated together with the controls Débora, Fanny and Santa Clara, in a randomized block design with two replications. The experiment was conducted in a research field of the UFV, from February to May 2007. We evaluated the disease severity, which is the percentage of diseased leaf area. The severity values were transformed into area under the disease progress curve (AUDPC), improving the result visualization. The analysis of variance and grouping of AUDPC means by the Scott-Knott test at 5 % significance were performed. The accessions BGH-2081, BGH-2034, BGH-700, BGH-2057, BGH-2035, BGH-2054, BGH-2018, BGH-2065, BGH-2008, and BGH-2032 had a lower mean AUDPC than the controls and are therefore indicated for future breeding programs.

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Morphological Characterization of Mycobacteriophage R1

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051281 ◽

1974 ◽

Vol 32 ◽

pp. 254-255

Author(s):

B. L. Soloff ◽

T. A. Rado

Keyword(s):

Carbon Source ◽

Stationary Phase ◽

Growth Phase ◽

Minimal Medium ◽

Morphological Characterization ◽

Logarithmic Growth Phase ◽

Complete Lysis ◽

Lysogenic Culture ◽

Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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AEM analysis of crystallization in Ti-based amorphous alloys

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100075142 ◽

1983 ◽

Vol 41 ◽

pp. 270-271

Author(s):

A.R. Pelton ◽

A.F. Marshall ◽

Y.S. Lee

Keyword(s):

Magnetic Properties ◽

Amorphous Alloys ◽

Amorphous Materials ◽

Isothermal Annealing ◽

Amorphous Matrix ◽

Nucleation And Growth ◽

Current Interest ◽

Amorphous Films ◽

Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

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