A microarray data analysis method to evaluate the impact of contaminants on wild animals

2010 ◽  
Vol 408 (23) ◽  
pp. 5824-5827
Author(s):  
Kei Nakayama ◽  
Itsuki C. Handoh ◽  
Shin-Ichi Kitamura ◽  
Eun-Young Kim ◽  
Hisato Iwata ◽  
...  
Keyword(s):  
Data Analysis ◽  
Microarray Data ◽  
Microarray Data Analysis ◽  
Wild Animals ◽  
Analysis Method ◽  
Data Analysis Method ◽  
The Impact

scholarly journals Oxidative Stress in Canine Histiocytic Sarcoma Cells Induced by an Infection with Canine Distemper Virus Led to a Dysregulation of HIF-1α Downstream Pathway Resulting in a Reduced Expression of VEGF-B In Vitro

Viruses ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 200 ◽  
Author(s):  
Federico Armando ◽  
Matteo Gambini ◽  
Attilio Corradi ◽  
Chiara Giudice ◽  
Vanessa Maria Pfankuche ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Data Analysis ◽  
Microarray Data ◽  
Canine Distemper Virus ◽  
Histiocytic Sarcoma ◽  
Microarray Data Analysis ◽  
Canine Distemper ◽  
The Impact ◽  
Dh82 Cells

Histiocytic sarcomas represent malignant tumors which require new treatment strategies. Canine distemper virus (CDV) is a promising candidate due to its oncolytic features reported in a canine histiocytic sarcoma cell line (DH82 cells). Interestingly, the underlying mechanism might include a dysregulation of angiogenesis. Based on these findings, the aim of the present study was to investigate the impact of a persistent CDV-infection on oxidative stress mediated changes in the expression of hypoxia-inducible factor (HIF)-1α and its angiogenic downstream pathway in DH82 cells in vitro. Microarray data analysis, immunofluorescence for 8-hydroxyguanosine, superoxide dismutase 2 and catalase, and flow cytometry for oxidative burst displayed an increased oxidative stress in persistently CDV-infected DH82 cells (DH82Ond pi) compared to controls. The HIF-1α expression in DH82Ond pi increased, as demonstrated by Western blot, and showed an unexpected, often sub-membranous distribution, as shown by immunofluorescence and immunoelectron microscopy. Furthermore, microarray data analysis and immunofluorescence confirmed a reduced expression of VEGF-B in DH82Ond pi compared to controls. In summary, these results suggest a reduced activation of the HIF-1α angiogenic downstream pathway in DH82Ond pi cells in vitro, most likely due to an excessive, unusually localized, and non-functional expression of HIF-1α triggered by a CDV-induced increased oxidative stress.

scholarly journals Entrepreneurial Education, Green Orientation Entrepreneur, dan Green Value terhadap Ecology Entrepreneurial Intention

2020 ◽  
Vol 2 (4) ◽  
pp. 914
Author(s):  
Edward Marchel Hugo ◽  
Kartika Nuringsih
Keyword(s):  
Data Analysis ◽  
University Students ◽  
Entrepreneurial Intention ◽  
Partial Least Square ◽  
Least Square ◽  
Analysis Method ◽  
Entrepreneurial Education ◽  
Data Analysis Method ◽  
The Impact

The purpose of this research is to determine the impact of entrepreneurialeducation, green orientantion entrepreneur, and green value against the ecology entrepreneurial intention of Tarumanagara University students in West Jakarta. The samples used in this research were 100 respondents who were students of Tarumanagara University in West Jakarta. This research uses Smart Partial Least Square (PLS)software version 3.0 as a data analysis method. The results of this research indicates that green orientation entrepreneur and green value affects the ecology entrepreneurial intention, while the entrepreneurial education does not affect the ecology entrepreneurial intention. Penelitian ini bertujuan untuk mengetahui pengaruh entrepreneurial education, green orientantion entrepreneur,dan green value terhadap ecology entrepreneurial intention pada mahasiswa Universitas Tarumanagara Jakarta. Jumlah sampel yang digunakan dalam penelitian ini sebanyak 100 responden yang merupakan mahasiswa/I Universitas Tarumanagara di Jakarta. Penelitian ini menggunakan Software Smart Partial Least Square (PLS)versi 3.0 sebagai metode analisis data. Hasil penelitian menunjukkan bahwa green orientantion entrepreneurdan green value berpengaruh terhadap ecology entrepreneurial intention, sedangkan entrepreneurial education tidak memiliki pengaruh terhadapecology entrepreneurial intention.

scholarly journals Enhancing Interdisciplinary Mathematics and Biology Education: A Microarray Data Analysis Course Bridging These Disciplines

2010 ◽  
Vol 9 (3) ◽  
pp. 217-226 ◽  
Author(s):  
Yolande V. Tra ◽  
Irene M. Evans
Keyword(s):  
Data Analysis ◽  
Microarray Data ◽  
Biology Education ◽  
Microarray Experiment ◽  
Educational Background ◽  
Microarray Data Analysis ◽  
Data Set ◽  
Set Up ◽  
Interdisciplinary Course ◽  
The Impact

BIO2010 put forth the goal of improving the mathematical educational background of biology students. The analysis and interpretation of microarray high-dimensional data can be very challenging and is best done by a statistician and a biologist working and teaching in a collaborative manner. We set up such a collaboration and designed a course on microarray data analysis. We started using Genome Consortium for Active Teaching (GCAT) materials and Microarray Genome and Clustering Tool software and added R statistical software along with Bioconductor packages. In response to student feedback, one microarray data set was fully analyzed in class, starting from preprocessing to gene discovery to pathway analysis using the latter software. A class project was to conduct a similar analysis where students analyzed their own data or data from a published journal paper. This exercise showed the impact that filtering, preprocessing, and different normalization methods had on gene inclusion in the final data set. We conclude that this course achieved its goals to equip students with skills to analyze data from a microarray experiment. We offer our insight about collaborative teaching as well as how other faculty might design and implement a similar interdisciplinary course.

scholarly journals Usaha Tiram Di Kelurahan Coppo Kecamatan Barru Kabupaten Barru

LaGeografia ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 80
Author(s):  
Marya Ulfah
Keyword(s):  
Data Analysis ◽  
Data Collection ◽  
Descriptive Analysis ◽  
Analysis Method ◽  
Data Analysis Method ◽  
Traditional Market ◽  
The Impact

This study is to find out: (1) An overview of traditional traders' income before the Grand Mall, (2) a description of the income of traditional traders after their Grand Mall, (3) The impact of the Grand Mall on the income of traditional market traders. The method used is descriptive quantitative and the population in this study are traditional traders who sell goods Related to the 288 merchants sold at the Grand Mall. The sample is 72 traders. Data collection techniques with observation, interviews, questionnaires / questionnaires and documentation with relevant parties. Data analysis method uses descriptive analysis. The results showed that the Grand Mall research influenced traditional market traders. Decreasing number, Decreasing the number of sales, Decreasing the number of sales of goods. Based on 72 traders only 23 agreed and 49 traditional traders were not approved by the modern market. Grand Mall is not as strong as its influence on the income of traditional market traders only increasing 23 traders from 72 traditional traders as a sample, or around 32%.

scholarly journals Impact of DNA microarray data transformation on gene expression analysis - comparison of two normalization methods.

2011 ◽  
Vol 58 (4) ◽  
Author(s):  
Marcin T Schmidt ◽  
Luiza Handschuh ◽  
Joanna Zyprych ◽  
Alicja Szabelska ◽  
Agnieszka K Olejnik-Schmidt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Data Analysis ◽  
Dna Microarray ◽  
Microarray Data ◽  
Normalization Method ◽  
Differentially Expressed ◽  
Microarray Data Analysis ◽  
Data Set ◽  
Normalization Methods ◽  
The Impact

Two-color DNA microarrays are commonly used for the analysis of global gene expression. They provide information on relative abundance of thousands of mRNAs. However, the generated data need to be normalized to minimize systematic variations so that biologically significant differences can be more easily identified. A large number of normalization procedures have been proposed and many softwares for microarray data analysis are available. Here, we have applied two normalization methods (median and loess) from two packages of microarray data analysis softwares. They were examined using a sample data set. We found that the number of genes identified as differentially expressed varied significantly depending on the method applied. The obtained results, i.e. lists of differentially expressed genes, were consistent only when we used median normalization methods. Loess normalization implemented in the two software packages provided less coherent and for some probes even contradictory results. In general, our results provide an additional piece of evidence that the normalization method can profoundly influence final results of DNA microarray-based analysis. The impact of the normalization method depends greatly on the algorithm employed. Consequently, the normalization procedure must be carefully considered and optimized for each individual data set.

scholarly journals KONSERVASI ALAM DALAM NOVEL KEKAL KARYA JALU KANCANA (KAJIAN EKOKRITIK)

Sirok Bastra ◽  
2020 ◽  
Vol 8 (2) ◽  
Author(s):  
Mamad Ahmad ◽  
Asep Supriyadi
Keyword(s):  
Content Analysis ◽  
Data Analysis ◽  
Nature Reserve ◽  
Analysis Method ◽  
Mining Company ◽  
Data Analysis Method ◽  
Data Source ◽  
The Impact

Novel Kekal karya Jalu Kancana menceritakan kegiatan eksploitasi yang dilakukan oleh perusahaan tambang panas bumi di Kawasan Cagar Alam Kamojang. Eksploitasi berdampak pada kerusakan alam dan kepunahan kekayaan flora dan fauna yang selama ini menjadi bagian keragaman hayati dunia. Penelitian ini membahas kerusakan dan upaya yang dilakukan oleh konservasionis terhadap kerusakan alam. Penelitian ini bertujuan untuk mendeskripsikan bentuk kerusakan hutan dan perjuangan kaum konservasi dalam menjaga dan melestarikan hutan berdasarkan kajian ekokritik Greg Garrard. Metode analisis data yang digunakan dalam penelitian ini adalah interpretasi dan pemahaman dengan analisis konten. Sumber data penelitian ini ialah novel dengan judul Kekal karya Jalu Kancana yang diterbitkan oleh Buku Mojok di Sleman Yogyakarta tahun 2019. Hasil analisis menunjukkan dampak dari eksploitasi telah mengakibatkan kerusakan hutan secara masif di sebagian wilayah hutan Indonesia umumnya dan Kawasan Cagar Alam Kamojang pada khususnya. Perlawanan yang dilakukan bersifat ideologis melalui tulisan yang kritis terhadap pemerintah.Novel Kekal by Jalu Kancana tells about the exploitation activities carried out by a geothermal mining company in the Kamojang Nature Reserve. Exploitation has an impact on the destruction of nature and the extinction of flora and fauna that have been part of the world's biodiversity. This study discusses the damage and efforts made by conservationists. This study aims to describe the form of forest destruction and the struggle of conservationists in protecting and conserving forests based on Greg Garrard's ecocritical study. The data analysis method used in this research is interpretation and understanding with content analysis. The data source for this research is a novel entitled Kekal by Jalu Kancana, published by Buku Mojok in Sleman Yogyakarta in 2019. The results of the analysis show that the impact of exploitation has resulted in massive forest destruction in parts of Indonesia's forest in general and in the Kamojang Nature Reserve in particular. Therefore, there are efforts made by conservationists to restore forest harmony.

BANK SPÓŁDZIELCZY (COOPERATIVE BANK) AS A GUARANTOR OF THE STABILISATION OF THE LOCAL FINANCIAL SYSTEM1

2019 ◽  
pp. 173-179
Author(s):  
Andrzej Pawlik
Keyword(s):  
Data Analysis ◽  
Banking Sector ◽  
Statistical Data ◽  
Local Development ◽  
Banking System ◽  
Analysis Method ◽  
Statistical Data Analysis ◽  
Data Analysis Method ◽  
Strong Position ◽  
Cooperative Banks

Urgency of the research. One of the most essential sources of supporting regional and local development is the banking system. Target setting. The study presented describes cooperative banking, represented by Bank Polskiej Spółdzielczości S.A. and Bank Spółdzielczy w Kielcach. The use of the statistical data analysis method allowed to demonstrate the strong position of cooperative banking in the market, fostering regional and local development. Actual scientific researches and issues analysis. The foundations for the modern cooperative banking sector were laid by cooperative financial organisations functioning more than 150 years ago [Pawlik, 2017, s. 152]. Its history is connected with difficulties faced in the period of partitions, work at the foundations after the end of World War I and Poland’s regaining its national independence. Uninvestigated parts of general matters defining. At present, cooperative banking functions as a result of the adoption by the Sejm of the Republic of Poland on 7 December 2000 of the act on the functioning of cooperative banks, their associations and associating banks, which ensured new legal conditions for the functioning of the sector2. The research objective. The article formulates the hypothesis that nowadays activities of cooperative banks will contribute to regional and local development. The statement of basic materials. One of the most essential sources of supporting regional and local development is the banking system. This system can guarantee the stabilisation of the local financial system. By supporting the development of regional and local entrepreneurship through loans, investment activities of the banks and financial and investment consulting, it will determine the identity of the region concerned. Conclusions. The use of the statistical data analysis method allowed to demonstrate the strong position of cooperative banking in the market, fostering regional and local development.

PENGARUH KUALITAS LAYANAN DAN NILAI PELANGGAN TERHADAP KEPUASAN PELANGGAN

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Ismail Razak, SE., MS. ◽  
Silviana Fadilla Prasevie
Keyword(s):  
Data Analysis ◽  
Linear Regression ◽  
Service Quality ◽  
Multiple Linear Regression ◽  
Customer Value ◽  
Sampling Method ◽  
Analysis Method ◽  
Simple Linear Regression ◽  
Central Java ◽  
Data Analysis Method

The purpose of this study was to analize the impacts of service quality and customer value on the satisfaction of customers. Questionnaires are used to collect data from Boks Café customer in Bumiayu, Kabupaten Brebes, Central java by using scale of Likert. In this study, accident sampling method was used and data analysis method was simple linear regression and multiple linear regression. The results of this study indicated that service quality and customer value positively and significant influenced the satisfaction of customers. The conclution of this study is that customer value was dominant than service quality in influencing the satisfaction of customers

PENGARUH KUALITAS LAYANAN DAN CITRA PERUSAHAAN TERHADAP KEPUASAN NASABAH

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Ismail Razak, SE., MS. ◽  
Elza Finnora
Keyword(s):  
Data Analysis ◽  
Linear Regression ◽  
Multiple Linear Regression ◽  
Sampling Method ◽  
Services Quality ◽  
Corporate Image ◽  
Primary Data ◽  
Analysis Method ◽  
Simple Linear Regression ◽  
Data Analysis Method

The aim of this study was to analize the influence of services quality and corporate image on the satisfaction of customers. Primary data was obtained from customers of Standard Chartered Bank through admission filling of questionnaire by using scale of Likert. In this study, accident sampling method was used and data analysis method was simple linear regression and multiple linear regression. The results of this study indicated that services quality and corporate image positively and significant influenced the satisfaction of customers. The conclution of this study is that services quality was dominant than corporate image in influencing the satisfaction of customers.

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