scholarly journals Dual-function oleaginous biocatalysts for non-sterile cultivation and solvent-free biolipid bioextraction to reduce biolipid-based biofuel production costs

2021 ◽  
Vol 758 ◽  
pp. 143969
Author(s):  
Myung Hwangbo ◽  
Jason J. Gill ◽  
Ry Young ◽  
Kung-Hui Chu
2017 ◽  
Vol 33 (5) ◽  
pp. 2257-2262 ◽  
Author(s):  
Pakpoom Sangdara ◽  
Maliwan Subsadsana ◽  
Chalerm Ruangviriyachai

2009 ◽  
Vol 8 (1) ◽  
pp. 84 ◽  
Author(s):  
K. C. C. Morais ◽  
R. L. L. Ribeiro ◽  
K. R. Santos ◽  
D. M. Taher ◽  
A. B. Mariano ◽  
...  

The Brazilian National Program for Biofuel Production has been encouraging diversification of feedstock for biofuel production. One of the most promising alternatives is the use of microalgae biomass for biofuel production. The cultivation of microalgae is conducted in aquatic systems, therefore microalgae oil production does not compete with agricultural land. Microalgae have greater photosynthetic efficiency than higher plants and are efficient fixing CO2. The challenge is to reduce production costs, which can be minimized by increasing productivity and oil biomass. Aiming to increase the production of microalgae biomass, mixotrophic cultivation, with the addition of glycerol has been shown to be very promising. During the production of biodiesel from microalgae there is availability of glycerol as a side product of the transesterification reaction, which could be used as organic carbon source for microalgae mixotrophic growth, resulting in increased biomass productivity. In this paper, to study the effect of glycerol in experimental conditions, the batch culture of the diatom Phaeodactylum tricornutum was performed in a 2-liter flask in a temperature and light intensity controlled room. During 16 days of cultivation, the number of cells per ml was counted periodically in a Neubauer chamber. The calculation of dry biomass in the control experiment (without glycerol) was performed every two days by vacuum filtration. In the dry biomass mixotrophic experiment with glycerol concentration of 1.5 M, the number of cells was assessed similarly in the 10th and 14th days of cultivation. Through a volume element methodology, a mathematical model was written to calculate the microalgae growth rate. Was used an equation that describes the influence of irradiation and concentration of nutrients in the growth of microalgae. A simulation time of 16 days was used in the computations, with initial concentration of 0.1 g l-1. In order to compare simulation data with experimental data, we calculated the dry weight in 8 points in the course of sixteen days. In this way, it was possible to assess graphically biomass concentration versus time through the experiments and by numerical simulation. It was identified that the simulation results were consistent with the experiments and that the addition of glycerol greatly influenced the growth of microalgae. In the present analysis, the glycerol added increased 30% in biomass.


2020 ◽  
Vol 197 ◽  
pp. 05002
Author(s):  
Elena Cerruti ◽  
Francesca Di Gruttola ◽  
Giancarlo Lauro ◽  
Teresa Dina Valentini ◽  
Paolo Fiaschi ◽  
...  

The present contribution deals with a comprehensive analysis of the available feedstocks and the appropriate technologies to produce advanced biofuels. The analysis is focused on the EU countries, since they adopted policy measures able to promote advanced biofuels as a strategic solution for a competitive and sustainable transport sector. In this regard, four classes of feedstocks have been taken into account: wastes, vegetable oils, agricultural and forestry residues. Their availability is studied with the aim to respect the European targets in terms of emissions without neglecting possible negative impacts on environment and biodiversity. A metric for the classification of the different solutions is proposed on the basis of feedstocks availability, technology readiness levels (TRL), quality of the produced biofuel as well as feedstock and production costs. It is possible to conclude that, even if the several interesting alternatives currently available have a high ranking in the proposed metric and must be taken in consideration, green diesel is today the most convenient solution for producing advanced biofuel without risks of technological failures and feedstocks shortage. This analysis can provide insights to encourage the development of advanced biofuels in EU, especially for some of the Member States as Germany, France and Italy.


Author(s):  
V. L. Nosko ◽  
O. V. Pavliv ◽  
A. Iu. Linnik

Energy crops are grown for energy purposes. Traditionally energy crops are corn and sugarcane which are grown for industrial scale ethanol, rapeseed for producing biodiesel, annual and perennial grasses, for example cane, miscanthus, cereal straw, as well as fast-growing tree crops for biomass production. The most interesting for the temperate climate zone of Europe are the fast-growing willow species. The interest in growing energy crops, which can be used as a renewable energy source, in European countries arose in the 1970s, which was related with rising prices for traditional energy sources. The growth of energy crops has been fueled by political decisions at the international level, in particular by documents such as the Renewable Energy Development Plan for Europe and the Kyoto Protocol. After some recession, landing areas for energy crops in the EU and North America have been stabilized. The fastest growing willow occupies the largest area in Europe. The average yield of willow wood in our experiments was about 50 tons per hectare at a moisture content of 45%, with a three-year biomass harvest cycle or 9.2 tons per year and dry matter from 9 to 15 tons per year per dry biomass, in depending on the conditions of cultivation, soil, clone. The weighted average cost of one ton of willow wood with a moisture content of 10% at an area of 100 hectares of plantation for its lifetime (22 years) will be $ 30.5. The cost of growing willow, transporting and shredding timber at a plantation area of 30 hectares is about $ 510 per hectare. About half of all biofuel production costs are depreciation deductions for the operation of special planting and harvesting equipment. The expansion of the plantation area 3–4 times compared to the base variant (30 hectares) allows to increase the profitability of energy production by 30–50%. The return on initial costs required to organize a willow plantation depends on the use of biomass. When replacing wood with traditional energy sources (natural gas), according to our calculations, the simple payback period is 3.8 years and the discounted time is 4.7 years, which corresponds to the time of harvesting the first biomass crop. With the direct sale of biomass on the market in the payback period increases to 6–11 years, which corresponds to the second or third harvesting period (with a three-year cycle). The unit cost of energy derived from willow wood is lower relatively to other energy crops, but 1.5 times and 1.8 times higher than that of natural marsh vegetation and straw, respectively. However, the additional interest in planting willow is due to their conservation value. The main indicators for calculating cost-effectiveness have been taken experimentally. The higher combustion heat of the above-ground part of the willow tree stand averaged 18500 kJ/kg. This is in line with the results obtained by other researchers for willow wood. The maximum specific heat of combustion of willow wood according to the results of experiments carried out in Sweden ranged from 18.3 to 19.7 MJ/kg, depending on the harvesting time and the willow clones. Therefore, we can confidently say that to grow energy willow is expediently and cost-effectively.


2021 ◽  
Vol 11 (15) ◽  
pp. 6756
Author(s):  
Enrique Gonzalez Bautista ◽  
Céline Laroche

In recent decades and to deal with the scarcity of fossil fuels, many studies have been developed in order to set up a sustainable biofuel production sector. This new sector must be efficient (high productivity), economically profitable (low production costs and therefore acceptable fuel prices), and ethical (low carbon balance, no competition with food resources). The production of bioethanol is based on the fermentation of reserve sugars, accumulated in the form of starch in microalgae and glycogen in cyanobacteria. The advantage of this bioenergy production route lies in the fact that the post-crop fermentation process is at the industrial stage since it has already been tested for many years for the production of bioethanol from agricultural resources. One of the most cultivated cyanobacteria is Arthrospira (“Spirulina”) and its production is also already at industrial scale. Depending on the cultivation conditions, this cyanobacteria is able to accumulate up to 65% DW (dry weight) of glycogen, making it a feasible feedstock for bioethanol production. The aim of this review is to provide a clear overview of these operating conditions for glycogen accumulation.


Author(s):  
Aurelio Lamare Soares Murta ◽  
Marcos Aurelio Vasconcelos De Freitas

Resumo Este trabalho tem por objetivo analisar a possibilidade de produção de biodiesel para uso em veículos de frota cativa em unidades militares da Marinha, a partir do óleo residual de fritura utilizado nas cozinhas das unidades militares. Para tanto, foi realizado um levantamento acerca da disponibilidade de óleo nas principais unidades militares da Região Metropolitana do Rio de Janeiro e modelada uma rede de coleta deste material para encaminhamento a um centro de armazenagem e produção do biocombustível definido pelo próprio estudo. Para tanto foram levados em consideração aspectos de localização geográfica, bem como a malha viária disponível. Foram realizados, também, cálculos de custos de produção do biodiesel e dos insumos industrializados necessários ao processo. Ao final, foi possível calcular o preço final por litro (preço unitário) de biodiesel produzido e a economia resultante deste processo, bem como as possibilidades do uso deste biocombustível numa frota cativa experimental de veículos militares.   Abstract This study aims to examine the possibility of producing biodiesel for use in fleet vehicles captive in military units of the Navy, from the residual oil used for frying in the kitchens of military units. For this, we conducted a survey on the availability of oil in the major military units in the metropolitan region of Rio de Janeiro and modeled a network of collecting this material for referral to a storage center and biofuel production set by the study itself. For both aspects were taken into account geographic location and the road network available. Were performed also calculations of the production costs of biodiesel and industrial inputs needed for the procedure. In the end, it was possible to calculate the final price per liter (unit price) of biodiesel and economy resulting from this process and the possibilities of using this biofuel in a captive fleet of military vehicles.


2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Mahendra Rai ◽  
Júlio César dos Santos ◽  
Matheus Francisco Soler ◽  
Paulo Ricardo Franco Marcelino ◽  
Larissa Pereira Brumano ◽  
...  

AbstractIn spite of the limited sources of fossil fuels, energy demand has been considerably increased since the last century. The problems associated with global warming due to rising atmospheric greenhouse gas levels and scarcity of fossil fuels make it imperative to reduce our heavy dependency on fossil fuels. These reasons forced countries throughout the world to search for new fuel alternatives. Biofuel have gathered considerable attention due to their inherent benefits, like lower greenhouse gas emission, renewability, and sustainability. Commercially, biofuels are produced from vegetable oils, animal fats, and carbohydrates by using transesterification and fermentation. However, biofuel production suffers from high production costs and other technical barriers. Considering the environmental and economic issues, use of nanotechnology seems to be a viable solution. Nanoparticles have a number of interesting properties for the production of second-generation ethanol or transesterification of oils and fats to yield biodiesel. It is advantageous for recovery and reuse of catalysts. The present review discusses the role of nanotechnology in the production of bioethanol and biodiesel. Moreover, applications of nanoparticles for the production of biodiesel and second-generation ethanol with special reference to enzyme immobilization and chemical nano-catalysis have been described.


Atmosphere ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1289
Author(s):  
M. Anwar H. Khan ◽  
Sophia Bonifacio ◽  
Joanna Clowes ◽  
Amy Foulds ◽  
Rayne Holland ◽  
...  

An accelerating global energy demand, paired with the harmful environmental effects of fossil fuels, has triggered the search for alternative, renewable energy sources. Biofuels are arguably a potential renewable energy source in the transportation industry as they can be used within current infrastructures and require less technological advances than other renewable alternatives, such as electric vehicles and nuclear power. The literature suggests biofuels can negatively impact food security and production; however, this is dependent on the type of feedstock used in biofuel production. Advanced biofuels, derived from inedible biomass, are heavily favoured but require further research and development to reach their full commercial potential. Replacing fossil fuels by biofuels can substantially reduce particulate matter (PM), carbon monoxide (CO) emissions, but simultaneously increase emissions of nitrogen oxides (NOx), acetaldehyde (CH3CHO) and peroxyacetyl nitrate (PAN), resulting in debates concerning the way biofuels should be implemented. The potential biofuel blends (FT-SPK, HEFA-SPK, ATJ-SPK and HFS-SIP) and their use as an alternative to kerosene-type fuels in the aviation industry have also been assessed. Although these fuels are currently more costly than conventional aviation fuels, possible reduction in production costs has been reported as a potential solution. A preliminary study shows that i-butanol emissions (1.8 Tg/year) as a biofuel can increase ozone levels by up to 6% in the upper troposphere, highlighting a potential climate impact. However, a larger number of studies will be needed to assess the practicalities and associated cost of using the biofuel in existing vehicles, particularly in terms of identifying any modifications to existing engine infrastructure, the impact of biofuel emissions, and their chemistry on the climate and human health, to fully determine their suitability as a potential renewable energy source.


2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Mu-Rong Kao ◽  
Hsion-Wen Kuo ◽  
Cheng-Chung Lee ◽  
Kuan-Ying Huang ◽  
Ting-Yen Huang ◽  
...  

Abstract Background To produce second-generation biofuels, enzymatic catalysis is required to convert cellulose from lignocellulosic biomass into fermentable sugars. β-Glucosidases finalize the process by hydrolyzing cellobiose into glucose, so the efficiency of cellulose hydrolysis largely depends on the quantity and quality of these enzymes used during saccharification. Accordingly, to reduce biofuel production costs, new microbial strains are needed that can produce highly efficient enzymes on a large scale. Results We heterologously expressed the fungal β-glucosidase D2-BGL from a Taiwanese indigenous fungus Chaetomella raphigera in Pichia pastoris for constitutive production by fermentation. Recombinant D2-BGL presented significantly higher substrate affinity than the commercial β-glucosidase Novozyme 188 (N188; Km = 0.2 vs 2.14 mM for p-nitrophenyl β-d-glucopyranoside and 0.96 vs 2.38 mM for cellobiose). When combined with RUT-C30 cellulases, it hydrolyzed acid-pretreated lignocellulosic biomasses more efficiently than the commercial cellulase mixture CTec3. The extent of conversion from cellulose to glucose was 83% for sugarcane bagasse and 63% for rice straws. Compared to N188, use of D2-BGL halved the time necessary to produce maximal levels of ethanol by a semi-simultaneous saccharification and fermentation process. We upscaled production of recombinant D2-BGL to 33.6 U/mL within 15 days using a 1-ton bioreactor. Crystal structure analysis revealed that D2-BGL belongs to glycoside hydrolase (GH) family 3. Removing the N-glycosylation N68 or O-glycosylation T431 residues by site-directed mutagenesis negatively affected enzyme production in P. pastoris. The F256 substrate-binding residue in D2-BGL is located in a shorter loop surrounding the active site pocket relative to that of Aspergillus β-glucosidases, and this short loop is responsible for its high substrate affinity toward cellobiose. Conclusions D2-BGL is an efficient supplement for lignocellulosic biomass saccharification, and we upscaled production of this enzyme using a 1-ton bioreactor. Enzyme production could be further improved using optimized fermentation, which could reduce biofuel production costs. Our structure analysis of D2-BGL offers new insights into GH3 β-glucosidases, which will be useful for strain improvements via a structure-based mutagenesis approach.


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