Assessing the role of cold temperatures on irreversible membrane permeability of tertiary ultrafiltration treating municipal wastewater

The paper summarizes the results of the studies on radiation purification of polluted water and wastewater conducted in the author's laboratory in cooperation with other institutions. The removal of heavy metals (cadmium, lead, chromium and mercury) from water and wastewater, the purification of wastewater from surfactant and petroleum products, molasses distillery slops, municipal wastewater in the aerosol flow, river water from colored natural organic pollutants, wastewater of dyeing complex and paper mill, the decomposition of some dyes, hydrogen peroxide, chlorine-containing organic compounds, formic acid, etc. were investigated in detail. As a rule, electron-beam treatment in combination with ordinary methods (biological, coagulation, adsorption, flotation, etc.) was used. The main attention is paid to the mechanism of purification of the studied systems. The role of redox reactions of primary products of water radiolysis and secondary short-lived species formed from pollutants, formation of precipitates capturing the pollutants etc. is discussed.

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Connexin 43 Knockdown Induces Mitochondrial Dysfunction and Affects Early Developmental Competence in Porcine Embryos

Microscopy and Microanalysis ◽

10.1017/s1431927620000033 ◽

2020 ◽

Vol 26 (2) ◽

pp. 287-296

Author(s):

Kyung-Tae Shin ◽

Zheng-Wen Nie ◽

Wenjun Zhou ◽

Dongjie Zhou ◽

Ju-Yeon Kim ◽

...

Keyword(s):

Membrane Permeability ◽

Connexin 43 ◽

Molecular Mechanisms ◽

Developmental Competence ◽

Cell Junction ◽

Ros Generation ◽

Blastocyst Development ◽

Atp Production ◽

Early Embryos

AbstractConnexin 43 (CX43) is a component of gap junctions. The lack of functional CX43 induces oxidative stress, autophagy, and apoptosis in somatic cells. However, the role of CX43 in the early development of porcine embryos is still unknown. Thus, the aim of this study was to investigate the role of CX43, and its underlying molecular mechanisms, on the developmental competence of early porcine embryos. We performed CX43 knockdown by microinjecting dsRNA into parthenogenetically activated porcine parthenotes. The blastocyst development rate and the total number of cells in the blastocysts were significantly reduced by CX43 knockdown. Results from FITC-dextran assays showed that CX43 knockdown significantly increased membrane permeability. ZO-1 protein was obliterated in CX43 knockdown blastocysts. Mitochondrial membrane potential and ATP production were significantly reduced following CX43 knockdown. Reactive oxygen species (ROS) levels were significantly increased in the CX43 knockdown group compared to those in control embryos. Moreover, CX43 knockdown induced autophagy and apoptosis. Our findings indicate that CX43 is essential for the development and preimplantation of porcine embryos and maintains mitochondrial function, cell junction structure, and cell homeostasis by regulating membrane permeability, ROS generation, autophagy, and apoptosis in early embryos.

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Prominence of Oxidative Stress in the Management of Anti-tuberculosis Drugs Related Hepatotoxicity

Drug Metabolism Letters ◽

10.2174/1872312813666190716155930 ◽

2020 ◽

Vol 13 (2) ◽

pp. 95-101 ◽

Cited By ~ 1

Author(s):

Preena John ◽

Pravin P. Kale

Keyword(s):

Oxidative Stress ◽

Membrane Permeability ◽

Signaling Pathway ◽

Critical Condition ◽

Permeability Transition ◽

Review Article ◽

Present Review ◽

Membrane Permeability Transition ◽

Hepatic Glutathione

: Advanced medical services and treatments are available for treating Tuberculosis. Related prevalence has increased in recent times. Unfortunately, the continuous consumption of related drugs is also known for inducing hepatotoxicity which is a critical condition and cannot be overlooked. The present review article has focused on the pathways causing these toxicities and also the role of enzyme CYP2E1, hepatic glutathione, Nrf2-ARE signaling pathway, and Membrane Permeability Transition as possible targets which may help in preventing the hepatotoxicity induced by the drugs used in the treatment of tuberculosis.

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