Color and distance two-dimensional visual and homogeneous dual fluorescence analysis of pathogenic bacteria in clinical samples

2022 ◽  
pp. 131422
Author(s):  
Piaopiao Chen ◽  
Yue Wang ◽  
Yanming Meng ◽  
Yaqin He ◽  
Ying Xiong ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Fluorescence Analysis ◽  
Clinical Samples ◽  
Two Dimensional ◽  
Dual Fluorescence

scholarly journals Is Staphylococcus lugdunensis Significant in Clinical Samples?

2017 ◽  
Vol 55 (11) ◽  
pp. 3167-3174 ◽  
Author(s):  
Xavier Argemi ◽  
Yves Hansmann ◽  
Philippe Riegel ◽  
Gilles Prévost
Keyword(s):  
Pathogenic Bacteria ◽  
Clinical Manifestations ◽  
Clinical Samples ◽  
Opportunistic Pathogens ◽  
Coagulase Negative Staphylococci ◽  
Staphylococcus Lugdunensis ◽  
Content Type ◽  
Severe Infections ◽  
Microbiological Data

ABSTRACTThe implication of coagulase-negative staphylococci in human diseases is a major issue, particularly in hospital settings wherein these species often act as opportunistic pathogens. In addition, some coagulase-negative staphylococci such asS. lugdunensishave emerged as pathogenic bacteria, implicated in severe infections, particularly, osteoarticular infections, foreign-body-associated infections, bacteremia, and endocarditis.In vitrostudies have shown the presence of several putative virulence factors such as adhesion factors, biofilm production, and proteolytic factors that might explain clinical manifestations. Taken together, the clinical and microbiological data might change the way clinicians and microbiologists look atS. lugdunensisin clinical samples.

Development of multiplex PCR assay for rapid detection Listeria monocytogenes in clinical samples

2021 ◽  
Vol 30 (4) ◽  
pp. 20-26
Author(s):  
Le Thanh Huong ◽  
Ha Thi Phuong Mai ◽  
Hoang Thi Thu Ha ◽  
Nguyen Dong Tu ◽  
Bui Tien Sy ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Vulnerable Groups ◽  
Clinical Samples ◽  
Specific Gene ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Specificity And Sensitivity

Listeria monocytogenes is widely present in the natural environment. This bacteria can cause infections in both humans and animals. In humans, the most vulnerable groups to be infected with L. monocytogenes are the elderly, people with an impaired immune system and chronically illness, pregnant women, and newborn babies. The aim of this study was to develop a multiplex PCR assay for the rapid detection of L. monocytogenes in mock clinical samples. A pair of primers were designed for detection of L. monocytogenes based on prs, a Listeria genus specific gene, and hly, a hemolysin gene. The specificity of the primers were tested by using different L. monocytogenes strains and other common pathogenic bacteria. The results showed that L. monocytogenes strains were positive in the detection and other tested strains were negative in mock (spiked) clinical samples. The sensitivity of multiplex PCR assay was 102 CFU/ml per reaction. The specificity and sensitivity of multiplex PCR technology for detecting L. monocytogenes in mock (spiked) clinical samples were high, and the assay could be completed within 1.5 hours. Therefore, this established multiplex PCR provides a rapid and reliable method and will be useful for the detection of L. monocytogenes in mock clinical samples.

scholarly journals Trends in antibiotic resistance patterns of methicillin resistant and methicillin sensitive Staphylococcus aureus in Khwaja Yunus Ali Medical College Hospital

KYAMC Journal ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Abdullah Akhtar Ahmed ◽  
Md Abdus Salam ◽  
Md Shakhaowat Hossain ◽  
Md Babul Aktar ◽  
Nusrat Akhtar Juyee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Current Status ◽  
Clinical Samples ◽  
Sensitivity Testing ◽  
Methicillin Resistant ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

Background: Antimicrobial resistance in pathogens is a growing concern for health. It is a great concern around the globe about the threat of increasing antimicrobial resistance. In response to this concerns, medical experts have proposed initiatives to curtail the spread of antimicrobial resistance in pathogenic bacteria. We have taken this surveillance study in KYAMCH to contain the spread of antimicrobial resistance.Objectives: To determine the prevalence of MRSA from different clinical samples and to record its current status to commonly used anti Staphylococcus antibiotics.Methods: The clinical samples were taken to the laboratory and within four hours cultured for isolates and identification pathogens. Finally, antibiotic sensitivity testing of the isolated Staphylococcus aureus was performed. Results: More than 80% of MRSA isolates were resistant to ampicillin (98%) followed by amoxicillin, cefixime and azithromycin with 94%, 93.5% and 85% respectively and maximum sensitivity toward tobramycin with 86% followed by 85% gentamicin and 79% meropenem.Conclusion: The detected trend in antibiotic resistance patterns of methicillin-resistant and methicillin-sensitive Staphylococcus aureus in this study is alarming. This has created a huge clinical burden in the hospital settings as well as in the community.KYAMC Journal Vol. 9, No.-1, April 2018, Page 6-10

scholarly journals Molecular beacon assay development for Severe Acute Respiratory Syndrome Coronavirus 2 detection

2021 ◽  
Author(s):  
Josué Carvalho ◽  
Jéssica Lopes Nunes ◽  
Joana Figueiredo ◽  
Tiago Santos ◽  
André Miranda ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Point Of Care ◽  
Total Duration ◽  
Fluorescence Analysis ◽  
Population Level ◽  
Clinical Samples ◽  
Test Duration ◽  
Upper Respiratory Tract ◽  
Testing Strategy ◽  
Two Stage

The fast spread of SARS-CoV-2 has led to a global pandemic, calling for fast and accurate assays to allow infection diagnosis and prevention of transmission. We aimed to develop a molecular beacon (MB)-based detection assay for SARS-CoV-2, designed to, detect the ORF1ab and S genes, proposing a two-stage COVID-19 testing strategy, using MBs to detect the presence of target amplicons by fluorescence analysis. Two MBs were designed, optimized in terms of concentration, fluorescence plateaus of hybridization, reaction temperature and best real-time results. A total of 450 nasopharyngel and throat swab samples (418 positive and 32 negative) were tested with the MB assay and the fluorescence levels compared with the cycle threshold (Ct) values obtained from a commercial RT-PCR test in terms of test duration, sensitivity and specificity. Our results show that the samples with higher fluorescence levels correspond to those with low Ct values, suggesting a correlation between viral load and increased MB fluorescence. The proposed assay represents a fast (total duration of 2 h 20 min including amplification and fluorescence reading stages) and simple way of detecting SARS-CoV-2 in clinical samples from the upper respiratory tract. Our two-stage testing strategy is suitable for further development into a point-of-care assay and potentially scalable to population level.

scholarly journals STUDY THE ANTIMICROBIAL AND ANTIADHESIVE ACTIVITY OF PURIFIED BIOSURFACTANT PRODUCED FROM LACTOBACILLUS PLANTARUM AGAINST PATHOGENIC BACTERIA

2021 ◽  
Vol 52 (5) ◽  
pp. 1194-1206
Author(s):  
A . A. Jameel ◽  
N. H. Haider
Keyword(s):  
Surface Tension ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Lower Surface ◽  
Solvent System ◽  
Inhibitory Effect ◽  
Clinical Samples ◽  
Adhesion Activity ◽  
Natural Media

This study was aimed to purification the biosurfactant that produced from Lactobacillus plantarum isolated from clinical samples of iraqi healthy women by column chromatography through silica gel column (3.5 × 30 cm) using solvent system (chloroform : methanol, 2:1) and characterization the purified product by Thin layer chromatography (TLC), Fourier Transform Infrared Red spectroscopy (FTIR) and Gas chromatography (GC) mass then evaluated its antibacterial and anti-adhesive activity. The results shown the higher emulsification activity (E24%= 63) and lower the surface tension to 23 mN/m in synthetic MSM, while the natural media gave (E24% =71) and lower surface tension to 19 mN/m. The using TLC technique indicated presence of lipopeptide in the biosurfactant with Rf value = 0.82. Antibacterial  and antiadhesion activities were evaluated against some pathogenic  bacteria, including Pseudomonas aureginosa and Staphylococcus aureus. The results showed a higher inhibitory effect of biosurfactant  at concentration 200 mg/ml on  S. aureus and P. aeruginosa with the inhibition zone 27 mm and 33 mm respectively in BCDFTM media. While purified  biosurfactant with concentration 200 mg/ml produced in MSM media had an effect on S. aureus and P. aeruginosa with the inhibition zone 21 mm, and 18 mm respectively. The anti-adhesion activity of purified biosurfactant against p.aeruginosa ranging from (34.70 ± 5.256) to (11.7 ± 9.7) produced from natural media BCDFTM  and was higher than the anti-adhesion activity of purified biosurfactant against the same pathogen ranging from (46.95 ± 7.37) to (20.15 ± 2.805)  produced in MSM.

scholarly journals STUDY THE INHIBITION ACTIVITY OF PURIFIED BACTERIOCIN FROM LOCAL ISOLATION Lactococcuslactis ssp. lactisagainst SOME PATHOGENIC BACTERIAL SPECIES ISOLATED FROM CLINICAL SAMPLES

2020 ◽  
Vol 12 (2) ◽  
pp. 34-49
Author(s):  
علاء حسين هامل1 ◽  
◽  
جيهان عبد الستار سلمان2 ◽  
رغد أكرم عزيز3 ◽  
◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Gel Filtration ◽  
Raw Milk ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Inhibition Activity ◽  
E Coli ◽  
The One

This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.

Two novel lanthanide(III) organic frameworks based on a biphenyltetracarboxylate ligand: synthesis, structure and magnetic and luminescence properties

2018 ◽  
Vol 74 (3) ◽  
pp. 386-391 ◽  
Author(s):  
Hongguo Hao ◽  
Yuchen Wang ◽  
Suxian Yuan ◽  
Dacheng Li ◽  
Junshan Sun
Keyword(s):  
Solid State ◽  
Topological Analysis ◽  
Fluorescence Analysis ◽  
Two Dimensional ◽  
Tetracarboxylic Acid ◽  
X Ray Diffraction ◽  
X Ray ◽  
Lanthanide Coordination Polymers ◽  
Magnetic Investigation ◽  
Solvothermal Conditions

Two new two-dimensional lanthanide coordination polymers, namely poly[[tetra-μ2-acetato-tetraaquabis(μ4-biphenyl-3,3′,5,5′-tetracarboxylato)tetrakis(dimethylacetamide)tetraterbium(III)] pentahydrate], {[Tb4(C16H6O8)2(C2H3O2)4(C4H9NO)4(H2O)4]·5H2O}n, (1), and poly[[tetra-μ2-acetato-tetraaquabis(μ5-biphenyl-3,3′,5,5′-tetracarboxylato)tetrakis(dimethylacetamide)tetraeuropium(III)] tetrahydrate], {[Eu4(C16H6O8)2(C2H3O2)4(C4H9NO)4(H2O)4]·4H2O}n, (2), have been synthesized from biphenyl-3,3′,5,5′-tetracarboxylic acid (H4bpt) and Ln(NO3)3·6H2O (Ln = Tb and Eu) under solvothermal conditions. Single-crystal X-ray structure analysis shows that the two compounds are isostructural and crystallize in the monoclinicP21/nspace group. The crystal structures are constructed from bpt4−ligands (as linkers) and {Ln2(μ2-CH3COO)2} building units (as nodes), which topological analysis shows to be a (4,6)-connected network withsqltopology. Compounds (1) and (2) have been characterized by elemental analysis, IR spectroscopy, powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA) and fluorescence analysis in the solid state. In addition, a magnetic investigation shows the presence of antiferromagnetic interactions in compound (1).

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