Impact of lincosamides antibiotics on the composition of the rat gut microbiota and the metabolite profile of plasma and feces

Abstract Background Gut microbiota and their metabolites were associated with obesity. Our previous study showed that maternal body fat percentage increased from days 45 to 110 of gestation in a Huanjiang mini-pig model. Thus, 16S rRNA sequencing and metabonomic techniques were used to investigate the changes of maternal gut microbiota composition and microbial metabolite profile from days 45 to 110 of gestation. Results The abundances of Clostridium_sensu_stricto_1, Romboutsia, Turicibacter, and Streptococcus in jejunum contents were higher in day 110 than those in day 45 or 75 of gestation. In ileum, the abundance of Streptococcus was the highest (P < 0.05) at day 110 of gestation, as well as the metabolism function of jejunal and ileal microbiota. The ileal butyrate and acetate concentrations were the highest at day 45 and day 110 of gestation, respectively. In colon, the concentrations of cadaverine and spermine were the highest (P < 0.05) at days 45 and 110 of gestation, respectively. Metabonomic analysis demonstrated that metabolic pathways including glutamine and glutamate metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alanine, aspartate, and glutamate metabolism changed during gestation. Conclusions Microbiota composition and metabolites changed dramatically from the early to the late pregnancy, which might be associated with the maternal fat accumulation.

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Microbiota and Metabolite Profiling as Markers of Mood Disorders: A Cross-Sectional Study in Obese Patients

Nutrients ◽

10.3390/nu14010147 ◽

2021 ◽

Vol 14 (1) ◽

pp. 147

Author(s):

Quentin Leyrolle ◽

Renata Cserjesi ◽

Romane Demeure ◽

Audrey M. Neyrinck ◽

Camille Amadieu ◽

...

Keyword(s):

Amino Acid ◽

Gut Microbiota ◽

Mood Disorders ◽

Profile Analysis ◽

Negative Mood ◽

Metabolite Profile ◽

Obese Patients ◽

Cross Sectional ◽

Increased Risk ◽

Obese Subjects

Obesity is associated with an increased risk of several neurological and psychiatric diseases, but few studies report the contribution of biological features in the occurrence of mood disorders in obese patients. The aim of the study is to evaluate the potential links between serum metabolomics and gut microbiome, and mood disturbances in a cohort of obese patients. Psychological, biological characteristics and nutritional habits were evaluated in 94 obese subjects from the Food4Gut study stratified according to their mood score assessed by the Positive and Negative Affect Schedule (PANAS). The fecal gut microbiota and plasma non-targeted metabolomics were analysed. Obese subjects with increased negative mood display elevated levels of Coprococcus as well as decreased levels of Sutterella and Lactobacillus. Serum metabolite profile analysis reveals in these subjects altered levels of several amino acid-derived metabolites, such as an increased level of L-histidine and a decreased in phenylacetylglutamine, linked to altered gut microbiota composition and function rather than to differences in dietary amino acid intake. Regarding clinical profile, we did not observe any differences between both groups. Our results reveal new microbiota-derived metabolites that characterize the alterations of mood in obese subjects, thereby allowing to propose new targets to tackle mood disturbances in this context. Food4gut, clinicaltrial.gov: NCT03852069.

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Environmental Chemical Diethylhexyl Phthalate Alters Intestinal Microbiota Community Structure and Metabolite Profile in Mice

mSystems ◽

10.1128/msystems.00724-19 ◽

2019 ◽

Vol 4 (6) ◽

Cited By ~ 3

Author(s):

Ming Lei ◽

Rani Menon ◽

Sara Manteiga ◽

Nicholas Alden ◽

Carrie Hunt ◽

...

Keyword(s):

Gut Microbiota ◽

Neurodevelopmental Disorders ◽

Developmental Disorders ◽

Chemical Exposure ◽

Metabolite Profile ◽

Environmental Chemicals ◽

Toxic Metabolite ◽

Environmental Chemical ◽

Diethylhexyl Phthalate

ABSTRACT Exposure to environmental chemicals during windows of development is a potentially contributing factor in gut microbiota dysbiosis and linked to chronic diseases and developmental disorders. We used a community-level model of microbiota metabolism to investigate the effects of diethylhexyl phthalate (DEHP), a ubiquitous plasticizer implicated in neurodevelopmental disorders, on the composition and metabolite outputs of gut microbiota in young mice. Administration of DEHP by oral gavage increased the abundance of Lachnoclostridium, while decreasing Clostridium sensu stricto. Addition of DEHP to in vitro-cultured cecal microbiota increased the abundance of Paenibacillus and Lachnoclostridium. Untargeted metabolomics showed that DEHP broadly altered the metabolite profile in the culture. Notably, DEHP enhanced the production of p-cresol while inhibiting butyrate synthesis. Metabolic model-guided correlation analysis indicated that the likely sources of p-cresol are Clostridium species. Monoculture of Lachnoclostridium bolteae confirmed that it is capable of producing p-hydroxyphenylacetic acid, the immediate precursor of p-cresol, and that the species’ growth is enhanced upon DEHP exposure. Taken together, these findings suggest a model where DEHP increases production of p-cresol, a bacterial metabolite linked with neurodevelopmental disorders, by expanding the abundance of species that synthesize the metabolite’s precursor. IMPORTANCE Several previous studies have pointed to environmental chemical exposure during windows of development as a contributing factor in neurodevelopmental disorders and correlated these disorders with microbiota dysbiosis; however, little is known about how the chemicals specifically alter the microbiota to interfere with development. The findings reported in this paper unambiguously establish that a pollutant linked with neurodevelopmental disorders can directly modify the microbiota to promote the production of a potentially toxic metabolite (p-cresol) that has also been correlated with neurodevelopmental disorders. Furthermore, we used a novel modeling strategy to identify the responsible enzymes and bacterial sources of this metabolite. To the best of our knowledge, the present study is the first to characterize the functional consequence of phthalate exposure on a developed microbiota. Our results suggest that specific bacterial pathways could be developed as diagnostic and therapeutic targets against health risks posed by ingestion of environmental chemicals.

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Characterization of the Rat Gut Microbiota via 16S rRNA Amplicon Library Sequencing

Methods in Molecular Biology - Rat Genomics ◽

10.1007/978-1-4939-9581-3_9 ◽

2019 ◽

pp. 195-212

Author(s):

Aaron C. Ericsson ◽

Susheel B. Busi ◽

James M. Amos-Landgraf

Keyword(s):

16S Rrna ◽

Gut Microbiota ◽

Amplicon Library ◽

Rat Gut

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Effect of organic food and moxibustion on diversity of rat gut microbiota

World Chinese Journal of Digestology ◽

10.11569/wcjd.v22.i31.4800 ◽

2014 ◽

Vol 22 (31) ◽

pp. 4800

Author(s):

Yang-Yang Jiang

Keyword(s):

Gut Microbiota ◽

Organic Food ◽

Rat Gut

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Limited beneficial effects of piceatannol supplementation on obesity complications in the obese Zucker rat: gut microbiota, metabolic, endocrine, and cardiac aspects

Journal of Physiology and Biochemistry ◽

10.1007/s13105-015-0464-2 ◽

2016 ◽

Vol 72 (3) ◽

pp. 567-582 ◽

Cited By ~ 17

Author(s):

E. Hijona ◽

L. Aguirre ◽

P. Pérez-Matute ◽

M. J. Villanueva-Millán ◽

A. Mosqueda-Solis ◽

...

Keyword(s):

Gut Microbiota ◽

Zucker Rat ◽

Beneficial Effects ◽

Obese Zucker Rat ◽

Obesity Complications ◽

Rat Gut

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Ganoderic acid A from Ganoderma lucidum ameliorates lipid metabolism and alters gut microbiota composition in hyperlipidemic mice fed a high-fat diet

Food & Function ◽

10.1039/d0fo00436g ◽

2020 ◽

Vol 11 (8) ◽

pp. 6818-6833

Author(s):

Wei-Ling Guo ◽

Jian-Bin Guo ◽

Bin-Yu Liu ◽

Jin-Qiang Lu ◽

Min Chen ◽

...

Keyword(s):

Lipid Metabolism ◽

Gut Microbiota ◽

High Fat Diet ◽

Ganoderma Lucidum ◽

Metabolite Profile ◽

Microbiota Composition ◽

High Fat ◽

Ganoderic Acid ◽

Gut Microbiota Composition

Ganoderic acid A from Ganoderma lucidum has the potential to prevent hyperlipidemia, modulates the composition of gut microbiota in hyperlipidemic mice, and significantly attenuates the liver metabolite profile in hyperlipidemic mice.

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Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota

Bioscience Biotechnology and Biochemistry ◽

10.1080/09168451.2019.1704618 ◽

2019 ◽

Vol 84 (4) ◽

pp. 824-831

Author(s):

Ryodai Takagaki ◽

Chiyo Yoshizane ◽

Yuki Ishida ◽

Takeo Sakurai ◽

Yoshifumi Taniguchi ◽

...

Keyword(s):

Gut Microbiota ◽

Inhibitory Effect ◽

Metabolite Production ◽

Rat Gut

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Impact of Vitamin D Deficit on the Rat Gut Microbiome

Nutrients ◽

10.3390/nu11112564 ◽

2019 ◽

Vol 11 (11) ◽

pp. 2564 ◽

Cited By ~ 2

Author(s):

Iñaki Robles-Vera ◽

María Callejo ◽

Ricardo Ramos ◽

Juan Duarte ◽

Francisco Perez-Vizcaino

Keyword(s):

Vitamin D ◽

Gut Microbiota ◽

Vitamin D Deficiency ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Microbiome Composition ◽

Gut Dysbiosis ◽

Β Diversity ◽

Α Diversity ◽

Rat Gut

Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis.

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