Background:
Different cellular responses influence the progress of cancer. In this study, we have investigated
the effect of hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in human
hepatocellular carcinoma (HepG2) cells.
Methods:
The effects of hydrogen peroxide and quercetin on cell viability, cell
cycle phases and oxidative stress related cellular changes were investigated. Cell viability was assessed by WST-1 assay.
Apoptosis rate, cell cycle phase changes and oxidative stress were measured by flow cytometry. Protein expressions of
p21, p27, p53, NF-Kβ-p50 and proteasome activity were determined by Western blot and fluorometry, respectively.
Results:
Hydrogen peroxide and quercetin treatment resulted in decreased cell viability and increased apoptosis in HepG2
cells. Proteasome activity was increased by hydrogen peroxide but decreased by quercetin treatment. Conclusion: Both
agents resulted in decreased p53 protein expression and increased cell death by different mechanisms regarding
proteostasis and cell cycle phases.
A dose-dependent effect of dimethyl sulfoxide on lipid content, cell viability and oxidative stress in 3T3-L1 adipocytes
