Immediate root canal disinfection with ultraviolet light: an ex vivo feasibility study

Author(s):  
Zvi Metzger ◽  
Hadar Better ◽  
Itzhak Abramovitz
2021 ◽  
Vol 12 (1) ◽  
pp. 44
Author(s):  
PujaChandrashekar Yavagal ◽  
ChandrashekarMurugesh Yavagal ◽  
ViplaviChavan Patil ◽  
NKiran Kumar ◽  
Madhu Hariharan ◽  
...  

2021 ◽  
Vol 10 (8) ◽  
pp. e32610817478
Author(s):  
Ana Luiza Rezende Fernandes ◽  
Ana Grasiela da Silva Limoeiro ◽  
Augusto Shoji Kato ◽  
Rina Andrea Pelegrine ◽  
Alexandre Sigrist de Martin ◽  
...  

The purpose of this ex vivo study was to evaluate the effectiveness of two irrigation techniques against E. Faecalis biofilm: conventional irrigation (CI) and passive ultrasonic irrigation (PUI). Furthermore, to investigate a possible cumulative effect of disinfection in the final irrigation, leaving the hypochlorite in the root canal for 2 or 5 minutes without agitation. A total of 63 premolars were divided into 9 groups (n = 7): Groups CI - 2.5 and CI - 5.25: Conventional irrigation with 2.5% and 5.25% NaOCl, respectively, a total of 6 mL for 1 min. Groups CI/PUI - 2.5 and CI/PUI - 5.25: CI + PUI with 2.5% and 5.25% NaOCl respectively, a total of 12 mL for 2 min. Groups CI/PUI - 2.5-2 (total of 14 mL for 4 min) and CI/PUI - 2.5-5 (total of 14 mL for 7 min): CI + PUI, plus final irrigation leaving the NaOCl 2,5% in the root canal for 2 or 5 minutes without agitation, respectively. Groups CI/PUI - 5,25-2 (total of 14 mL for 4 min) and CI/PUI - 5,25-5 (total of 14 mL for 7 minutes): CI + PUI, plus final irrigation leaving the NaOCl 5,25% in the root canal for 2 or 5 minutes without agitation, respectively. Control Group: CI + PUI, final irrigation leaving sterile saline solution for 5 min. The CI/PUI - 5,25 group showed a significantly greater reduction of E. faecalis than in the CI/PUI - 2.5 group. All other groups did not differ significantly from each other. The control group showed a less efficient reduction of E. faecalis. The CI associated with PUI was sufficiently able to reduce the bacterial load of E. faecalis. The final irrigation, leaving the hypochlorite in the root canal for 2 or 5 minutes without agitation did not demonstrate relevance in this study. Successive changes of the irrigating liquid, resulting in greater volume, demonstrated better results in disinfecting the root canal system.


2008 ◽  
Vol 34 (12) ◽  
pp. 1490-1496 ◽  
Author(s):  
Gaëlle Villette ◽  
Sheela Manek ◽  
Milos Legner ◽  
Edward D. Fillery ◽  
Calvin D. Torneck ◽  
...  

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1412
Author(s):  
Pilar Valderrama ◽  
Pilar Baca ◽  
Carmen Solana ◽  
Carmen María Ferrer-Luque

The relative citation rate (RCR) is a normalized article-level metric useful to assess the impact of research articles. The objective of this bibliometric study is to identify and analyze, in root canal disinfection, the 100 articles having the highest RCRs in the period 1990–2019, then compare them with the top 100 articles most cited. A cross-sectional study was performed, and the search strategy ((Disinfection AND root canal) AND ((“1990/01/01”[Date-Publication]: “2019/12/31”[Date-Publication]))) relied on PubMed (n = 4294 documents), and article data were downloaded from the iCite database. The 100 articles with the highest RCRs and the top 100 cited were selected and evaluated in bibliometric terms. Among the 100 articles with the highest RCRs, there were no differences in the three decades for RCRs values, but there were in citations, being 2000–2009 the most cited. The USA was the predominant country (n = 30), followed by Brazil (n = 14). The most frequent study designs were reviews (n = 27) and in vitro (n = 25) and ex vivo (n = 24) studies. All subfields were well represented, although they varied over time. In 2010–2019, regenerative procedures and irrigation/disinfection techniques were predominant. Considering the RCR’s top 100 articles, 76 were common with the 100 most cited articles. Using the RCR metric allowed us to identify influential articles in root canal disinfection, a research field with topics of significance that fluctuate over time. Compared to citations, RCR reduces the time from publication to detection of its importance for the readership and could be a valid alternative to citation counts.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beatriz H. D. Panariello ◽  
Justin K. Kindler ◽  
Kenneth J. Spolnik ◽  
Ygal Ehrlich ◽  
George J. Eckert ◽  
...  

AbstractRoot canal disinfection is of utmost importance in the success of the treatment, thus, a novel method for achieving root canal disinfection by electromagnetic waves, creating a synergistic reaction via electric and thermal energy, was created. To study electromagnetic stimulation (EMS) for the disinfection of root canal in vitro, single rooted teeth were instrumented with a 45.05 Wave One Gold reciprocating file. Specimens were sterilized and inoculated with Enterococcus faecalis ATCC 29,212, which grew for 15 days to form an established biofilm. Samples were treated with 6% sodium hypochlorite (NaOCl), 1.5% NaOCl 1.5% NaOCl with EMS, 0.9% saline with EMS or 0.9% saline. After treatments, the colony forming units (CFU) was determined. Data was analyzed by Wilcoxon Rank Sums Test (α = 0.05). One sample per group was scored and split for confocal laser scanning microscopy imaging. There was a significant effect with the use of NaOCl with or without EMS versus 0.9% saline with or without EMS (p = 0.012 and 0.003, respectively). CFUs were lower when using 0.9% saline with EMS versus 0.9% saline alone (p = 0.002). Confocal imaging confirmed CFU findings. EMS with saline has an antibiofilm effect against E. faecalis and can potentially be applied for endodontic disinfection.


2017 ◽  
Vol 22 (1) ◽  
pp. 547-547
Author(s):  
Frederico C. Martinho ◽  
Cinthya C. Gomes ◽  
Gustavo G. Nascimento ◽  
Ana P. M. Gomes ◽  
Fábio R. M. Leite

2012 ◽  
Vol 23 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Bruno Carvalho de Vasconcelos ◽  
Leonardo de Alencar Matos ◽  
Elilton Cavalcante Pinheiro-Júnior ◽  
Antônio Sérgio Teixeira de Menezes ◽  
Nilton Vivacqua-Gomes

This study evaluated the accuracy of three electronic apex locators (Root ZX, Novapex, and Justy II) in root canal length determinations using different apical file sizes, considering the apical constriction (AC) and the major foramen (MF) as anatomic references. The diameter of the apical foramina of 40 single-rooted teeth was determined by direct visual measurement and the master apical file was established. Electronic measurements were then performed using 3 instruments: the selected master apical file (adjusted file), one size smaller (intermediate file), and two sizes smaller (misfit file). The distances from the tip of files fixed in the canals to the MF and to the AC were measured digitally. Precision at AC and at MF for the misfit, intermediate and adjusted apical files was as follows: 80%/88%/83% and 78%/83%/95% (Root ZX); 80%/85%/80% and 68%/73%/73% (Novapex); and 78%/80%/78% and 65%/78%/70% (Justy II). Considering the mean discrepancies, statistically significant differences were found only for the adjusted file at MF, with Root ZX presenting the best results at MF. The chi-square test showed significant differences between the acceptable measurements at AC and at MF for the Justy II and Novapex (± 0.5 mm) regardless of file adjustment. Under the conditions of the present study, all devices provided acceptable electronic measurements regardless of file adjustment, except for Root ZX which had its performance improved significantly when the precisely fit apical file was used. Justy II and Novapex provided electronic measurements nearest to the AC.


2021 ◽  
Vol 9 (3) ◽  
pp. 517
Author(s):  
Mohamed El-Telbany ◽  
Gamal El-Didamony ◽  
Ahmed Askora ◽  
Eman Ariny ◽  
Dalia Abdallah ◽  
...  

Phage therapy is an alternative treatment to antibiotics that can overcome multi-drug resistant bacteria. In this study, we aimed to isolate and characterize lytic bacteriophages targeted against Enterococcus faecalis isolated from root canal infections obtained from clinics at the Faculty of Dentistry, Ismalia, Egypt. Bacteriophage, vB_ZEFP, was isolated from concentrated wastewater collected from hospital sewage. Morphological and genomic analysis revealed that the phage belongs to the Podoviridae family with a linear double-stranded DNA genome, consisting of 18,454, with a G + C content of 32.8%. Host range analysis revealed the phage could infect 10 of 13 E. faecalis isolates exhibiting a range of antibiotic resistances recovered from infected root canals with efficiency of plating values above 0.5. One-step growth curves of this phage showed that it has a burst size of 110 PFU per infected cell, with a latent period of 10 min. The lytic activity of this phage against E. faecalis biofilms showed that the phage was able to control the growth of E. faecalis in vitro. Phage vB_ZEFP could also prevent ex-vivo E. faecalis root canal infection. These results suggest that phage vB_ZEFP has potential for application in phage therapy and specifically in the prevention of infection after root canal treatment.


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