scholarly journals Pre-analytical stability of sorbitol dehydrogenase in equine heparinized plasma

2021 ◽  
pp. 105706
Author(s):  
N. Fouché ◽  
S. Oesch ◽  
V. Gerber ◽  
H. Richter ◽  
J. Howard ◽  
...  
Keyword(s):  
Sorbitol Dehydrogenase ◽  
Analytical Stability

Molecular cloning and biochemical characterization of a NAD-dependent sorbitol dehydrogenase from cold-adapted Pseudomonas mandelii

2021 ◽  
Author(s):  
Quynh DangThu ◽  
Thu-Thuy Nguyen ◽  
Sei-Heon Jang ◽  
ChangWoo Lee
Keyword(s):  
Biochemical Characterization ◽  
Size Exclusion ◽  
Sorbitol Dehydrogenase ◽  
High Catalytic Activity ◽  
Active Site Residues ◽  
Tertiary Structures ◽  
Cold Adapted ◽  
Pseudomonas Mandelii ◽  
Catalytic Active Site ◽  
Exclusion Chromatography

Abstract Sugar alcohols (polyols) have important roles as nutrients, anti-freezing agents, and scavengers of free radicals in cold-adapted bacteria, but the characteristics of polyol dehydrogenases in cold-adapted bacteria remain largely unknown. In this study, based on the observation that a cold-adapted bacterium Pseudomonas mandelii JR-1 predominantly utilized D-sorbitol as its carbon source, among the four polyols examined (D-galactitol, D-mannitol, D-sorbitol, or D-xylitol), we cloned and characterized a sorbitol dehydrogenase (SDH, EC 1.1.1.14) belonging to the short-chain dehydrogenase/reductase family from this bacterium (the SDH hereafter referred to as PmSDH). PmSDH contained Asn111, Ser140, Tyr153, and Lys157 as catalytic active site residues and existed as a ∼67 kDa dimer in size-exclusion chromatography. PmSDH converted D-sorbitol to D-fructose using NAD+ as a coenzyme and, vice versa, D-fructose to D-sorbitol using NADH as a coenzyme. PmSDH maintained its conformational flexibility, secondary and tertiary structures, and thermal stability at 4–25°C. At 40°C, PmSDH was rapidly denatured. These results indicate that PmSDH, which has a flexible structure and a high catalytic activity at colder temperatures, is well-suited to sorbitol utilization in the cold-adapted bacterium P. mandelii JR-1.

Activities of NADPH-dependent reductases and sorbitol dehydrogenase in canine and feline lenses

2000 ◽  
Vol 61 (10) ◽  
pp. 1322-1324 ◽  
Author(s):  
Diego Salgado ◽  
Rhea S. Forrer ◽  
Bernhard M. Spiess
Keyword(s):  
Sorbitol Dehydrogenase

scholarly journals Human erythrocyte sorbitol metabolism and the role of sorbitol dehydrogenase

Diabetologia ◽  
1988 ◽  
Vol 31 (10) ◽  
pp. 766-770 ◽  
Author(s):  
Y. Nagasaka ◽  
S. Fujii ◽  
T. Kaneko
Keyword(s):  
Human Erythrocyte ◽  
Sorbitol Dehydrogenase ◽  
Sorbitol Metabolism

Parallel-Process (Simultaneous) Machining and its Stability

1999 ◽  
Author(s):  
O. Burak Ozdoganlar ◽  
William J. Endres
Keyword(s):  
Initial Conditions ◽  
General System ◽  
Parallel Process ◽  
Analytical Stability ◽  
Multiple Processes ◽  
Or Practice ◽  
Machining System ◽  
Eigenvector Analysis ◽  
The Stability ◽  
Machining Operations

Abstract This paper presents a mathematical perspective, to complement the intuitive or practice-oriented perspective, to classifying machining operations as parallel-process (simultaneous) or single-process in nature. Illustrative scenarios are provided to demonstrate how these two perspectives may lead in different situations to the same or different conclusions regarding process parallelism. A model representation of a general parallel-process machining system is presented, based on which the general parallel-process stability eigenvalue problem is formulated. For a special simplified case of the general system, analytical methods are employed to derive a fully analytical stability solution. Thorough study of this solution through eigenvector analysis sheds light on some fundamental phenomena of parallel-process machining stability, such as dependence of the stability solution on phasing of the initial conditions (disturbances). This establishes the importance, when employing numerical time-domain simulation for such analyses, of specifying initial conditions for the multiple processes to be arbitrarily phased so that correct results are achieved across all spindle speeds.

Study of the nutritional dynamic in olive leaves: periods of analytical stability

2017 ◽  
Vol 7 ◽  
Author(s):  
Juana Nieto ◽  
Antonio García-Fuentes ◽  
Llenalia M. García ◽  
Emilia Fernández-Ondoño
Keyword(s):  
Nutritional Deficiencies ◽  
Winter Period ◽  
Olive Leaves ◽  
Nitrogen And Phosphorus ◽  
Analytical Stability ◽  
Opposite Behavior ◽  
Zinc Concentrations ◽  
Calcium Magnesium ◽  
Olive Trees ◽  
One Year

The aim of this study was to establish the evolution curves of macro- and micro-nutrients in olive leaves and the periods of time in which they are statistically stable and can be considered suitable for a nutritional diagnosis. The study was carried out in two farms of the province of Jaén (Andalucia, Southern Spain), with olive trees of the Picual variety, under an irrigation regime and no nutritional deficiencies over the entire year. The evolution curves of each nutrient had great similarities when comparing between farms and between periods. Only in some periods the farm “Poco Humo” had higher concentrations, probably due to the most favorable edaphic characteristics of this farm. Nitrogen and phosphorus showed minimum concentrations when the leaves were young and when they were one year old, and maximum concentrations during the winter. Potassium and boron showed higher concentrations when the leaves were young, and the concentrations decreased throughout the first year of life. Concentrations of calcium, magnesium and manganese had the opposite behavior: these accumulated in leaf until reaching maximum values in winter and then remained stable, with some oscillations but without statistically significant differences. No changes were observed in the zinc concentrations in the sampled periods. Periods of at least two consecutive months without statistically significant differences were found during the winter period for all elements except nitrogen. Analytical stability was observed from the second fortnight of May till the first fortnight of August for all elements except phosphorus and boron.

scholarly journals A structural-relational analysis of party dynamics in proxy wars

2018 ◽  
Vol 32 (4) ◽  
pp. 449-467 ◽  
Author(s):  
Vladimir Rauta
Keyword(s):  
Cost Benefit ◽  
Strategic Interaction ◽  
Analytical Framework ◽  
Power Cost ◽  
Relational Analysis ◽  
Analytical Stability ◽  
Proxy War ◽  
Novel Variant ◽  
Tentative Explanation ◽  
Conflict Research

Proxy wars are still under-represented in conflict research and a key cause for this is the lack of conceptual and terminological care. This article seeks to demonstrate that minimising terminological diffusion increases overall analytical stability by maximising conceptual rigour. The argument opens with a discussion on the terminological ambivalence resulting from the haphazard employment of labels referencing the parties involved in proxy wars. Here, the article introduces an analytical framework with a two-fold aim: to reduce label heterogeneity, and to argue in favour of understanding proxy war dynamics as overlapping dyads between a Beneficiary, a Proxy, and a Target. This is then applied to the issues of defining and theorising party dynamics in proxy wars. It does so by providing a structural-relational analysis of the interactions between the above-mentioned parties based on strategic interaction. It presents a tentative explanation of the proxy relationship by correlating the Beneficiary’s goal towards the Target with the Proxy’s preference for the Beneficiary. In adding the goal-preference relational heuristic, the article advances the recent focus on strategic interaction with a novel variant to explanations based on interest, power, cost–benefit considerations or ideology.

Haematological and serum chemistry profiles of ferrets (Mustela putorius furo)

1982 ◽  
Vol 16 (2) ◽  
pp. 133-137 ◽  
Author(s):  
Elsbeth J. Lee ◽  
W. E. Moore ◽  
H. C. Fryer ◽  
H. C. Minocha
Keyword(s):  
Alkaline Phosphatase ◽  
Weight Gain ◽  
Total Protein ◽  
Alanine Aminotransferase ◽  
Inorganic Phosphate ◽  
Sorbitol Dehydrogenase ◽  
Serum Chemistry ◽  
Mustela Putorius ◽  
Mustela Putorius Furo

Female, male and castrated male ferrets were studied. Weight gain plateaued at 28-weeks of age with males about 500 g heavier than females. No statistically significant differences in haematology were observed with age, but alkaline phosphatase and alanine aminotransferase levels fell while glucose increased. Haemolysis led to various changes including marked increases in total protein, albumin, inorganic phosphate and sorbitol dehydrogenase.

scholarly journals New insights into the evolutionary history of plant sorbitol dehydrogenase

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Yong Jia ◽  
Darren CJ Wong ◽  
Crystal Sweetman ◽  
John B Bruning ◽  
Christopher M Ford
Keyword(s):  
Evolutionary History ◽  
Sorbitol Dehydrogenase ◽  
History Of
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