Upregulation of CD4+CD8+ memory cells in the piglet intestine following oral administration of Bacillus subtilis spores combined with PEDV whole inactivated virus

2019 ◽  
Vol 235 ◽  
pp. 1-9 ◽  
Author(s):  
Lulu Huang ◽  
Jialu Wang ◽  
Yongheng Wang ◽  
En Zhang ◽  
Yuchen Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Oral Administration ◽  
Memory Cells

scholarly journals Estimation of permeability to bacteriophages of intestinal mucosa of chickens with eimeriosis

2019 ◽  
Vol 49 (2) ◽  
pp. 57-63
Author(s):  
N. V. Davydova ◽  
V. Yu. Koptev ◽  
Yu. N. Kozlova ◽  
L. I. Sulimova ◽  
V. N. Afonyushkin ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Oral Administration ◽  
Protective Effect ◽  
Intestinal Mucosa ◽  
Clinical Picture ◽  
Biological Material ◽  
Probiotic Strain ◽  
Field Isolates ◽  
Different Types ◽  
Inflammatory Processes

In the course of the study permeability of intestinal mucosa of chickens suffering from eimeriosis while treating them with various veterinary drugs, including probiotics, was evaluated. The simulation of a typical clinical picture of eimeriosis was carried out by oral administration of suspension with coccidial oocysts (1.6 × 105/head) using a probe. To create different forms and different intensity of inflammatory processes, chickens that received various anticoccidial preparations and probiotic strain Bacillus subtilis were infected with eimeria. According to the data from an autopsy, it was found that the use of a spore probiotic based on Bacillus subtilis and anticoccidial drugs containing robenidine hydrochloride and salinomycin had a positive protective effect when treating chickens from eimeriosis. A similar picture was observed when assessing permeability of intestinal mucosa as affected by bacteriophage, whereby permeability decreased with the use of probiotics and the above-mentioned active agents. In general, the decrease in productivity was significant in all groups. However, the effect of spore-based probiotics was quite pronounced against the background of eimeria polyresistance. In the situation where anticoccidial drugs are less effective, the use of a spore-based probiotic can have a noticeable protective effect. The effect of all anticoccidial drugs under study on the concentration of oocysts and the state of the mucosa was insignificant, which indicated polyresistance of different types of eimeria isolated from biological material to these drugs. The analysis of the intestinal mucosa integrity, based on the study of mucosa permeability to bacteriophages and a Johnson and Reid scoring procedure showed that a spore probiotic based on B. subtilis and anticoccidial drugs containing robenidine and salinomycin had the best protective effect against eimeriosis caused by field isolates of eimeria. When treating chickens suffering from eimeriosis caused by polyresistant forms of E. acervulina and E. tenella, it is advisable to use probiotics alongside with drugs based on robenidine and salinomycin.

scholarly journals Oral administration of recombinant Bacillus subtilis spores expressing mutant staphylococcal enterotoxin B provides potent protection against lethal enterotoxin challenge

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Zhile Xiong ◽  
Jialiang Mai ◽  
Fei Li ◽  
Bingshao Liang ◽  
Shuwen Yao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Oral Administration ◽  
Protective Efficacy ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Mutant Form ◽  
Humoral Immune ◽  
Enterotoxin B ◽  
Recombinant Bacillus Subtilis ◽  
Factor Α

AbstractPathogenicity of Staphylococcus aureus is induced by staphylococcal enterotoxin B (SEB). A mutant form of SEB (mSEB) is immunogenic as well as less toxic. Recombinant mSEB and SEB were expressed in pET28a prokaryotic plasmids. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in mSEB-stimulated macrophages were lower than those in SEB-stimulated macrophages (p < 0.001, p < 0.01 respectively). Using CotC as a fusion protein, we constructed recombinant Bacillus subtilis spores expressing mSEB on the spore surface and evaluated their safety and protective efficacy via mouse models. Oral administration of mSEB-expressing spores increased SEB-specific IgA in feces and SEB-specific IgG1 and IgG2a in the sera, compared with mice in naïve and CotC spore-treated groups (p < 0.001, p < 0.01, p < 0.001 respectively). Six weeks following oral dosing of recombinant spores, significant differences were not found in the serum biochemical indices between the mSEB group and the naïve and CotC groups. Furthermore, oral administration of mSEB spores increased the survival rate by 33.3% in mice intraperitoneally injected with 5 µg of wild-type SEB plus 25 µg lipopolysaccharide (LPS). In summation, recombinant spores stably expressing mSEB were developed, and oral administration of such recombinant spores induced a humoral immune response and provided protection against SEB challenge in mice.

scholarly journals Mucosal immune responses induced by oral administration recombinant Bacillus subtilis expressing the COE antigen of PEDV in newborn piglets

2019 ◽  
Vol 39 (3) ◽  
Author(s):  
Jialu Wang ◽  
Lulu Huang ◽  
Chunxiao Mou ◽  
En Zhang ◽  
Yongheng Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Immune Responses ◽  
Oral Administration ◽  
Oral Vaccine ◽  
Oral Immunization ◽  
Economic Losses ◽  
Antigen Delivery ◽  
Newborn Piglets ◽  
Mucosal Immune Responses ◽  
Recombinant Bacillus Subtilis

Abstract Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets and causes substantial economic losses in the world. PED virus (PEDV) spreads by fecal–oral contact and can be prevented by oral immunization. Therefore, it is necessary to develop an effective oral vaccine against PEDV infection. Currently, Bacillus subtilis as recombinant vaccine carrier has been used for antigen delivery and proved well in immune effect and safety. The present study evaluated the immunogenicity of recombinant Bacillus subtilis (B. subtilis-RC) in piglets via oral administration. After oral immunization in piglets, B. subtilis-RC significantly increased the local mucosal immune responses. Oral administration with B. subtilis-RC significantly improved the level of specific mucosal immunoglobulin A (IgA) antibodies against PEDV infection, through enlarging the area of Peyer’s patches (PPs) and increasing the number of ileum IgA+ secreting (SIgA) cells. In the meantime, B. subtilis-RC remarkably increased the number of intraepithelial lymphocytes (IELs). We also observed that oral administration of B. subtilis-RC significantly increased CD3+T lymphocytes’ numbers and up-regulated the ratio of CD4+/CD8+ T cells. Furthermore, high titers of specific serum immunoglobulin G (IgG) revealed satisfactory systemic immune response against PEDV infection. In summary, our study demonstrated that oral administration of B. subtilis-RC could trigger a high level of local and systemic immune responses and would be a promising candidate vaccine against PEDV infection in piglets.

scholarly journals Oral administration of Bacillus subtilis strain BSB3 can prevent heat stress-related adverse effects in rats

2014 ◽  
Vol 117 (5) ◽  
pp. 1463-1471 ◽  
Author(s):  
T. Moore ◽  
L. Globa ◽  
O. Pustovyy ◽  
V. Vodyanoy ◽  
I. Sorokulova
Keyword(s):  
Bacillus Subtilis ◽  
Heat Stress ◽  
Adverse Effects ◽  
Oral Administration ◽  
Subtilis Strain

scholarly journals Prevention and Curation of DSS-induced IBD in Mice With Bacillus Subtilis Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora

2020 ◽  
Author(s):  
Xuan Zhang ◽  
Yanjun Tong ◽  
Xiaomei Lyu ◽  
Jin Wang ◽  
Yuxue Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Inflammatory Response ◽  
Oral Administration ◽  
Intestinal Mucosa ◽  
Inflammatory Responses ◽  
Activity Index ◽  
Intestinal Flora ◽  
Fermented Milk ◽  
Mucosal Barrier ◽  
Therapeutic Effects

Abstract Background: The pathogenesis of inflammatory bowel disease (IBD) might be related to the local inflammatory damage and the dysbacteriosis of intestinal flora. Probiotics can regulate the intestinal flora and ameliorate IBD. The probiotic Bacillus subtilis strain B. subtilis JNFE0126 was used as the starter of fermented milk. However, the therapeutic effects of B. subtilis fermented milk on IBD remains to be explored.Methods：The therapeutic effect of the B. subtilis fermented milk on DSS-induced IBD model mice was evaluated. The disease activity index (DAI) and the pathological features of small intestinal and colonic mucosa were examined. For exploring the action mechanism of B. subtilis, immunohistochemical staining and western-blotting were used to analyse the expression of the pro-inflammatory/anti-inflammatory cytokines, the proliferation of the intestinal stem cells, and the reconstruction of the mucosa barrier. The alteration of gut microbiota was investigated by taxonomic analysis.Results: The DAI of IBD was significantly decreased through oral administration of B. subtilis (JNFE0126) fermented milk, and the intestinal mucosa injury was attenuated. Moreover, B. subtilis could reduce the inflammatory response of the intestinal mucosa, induce proliferation of the intestinal stem cell, and promote reconstruction of the mucosal barrier. Furthermore, B. subtilis could rebalance the intestinal flora, increasing the abundance of Bacillus, Alistipes and Lactobacillus, while decreasing the abundance of Escherichia and Bacteroides.Conclusion: Oral administration of the B. subtilis fermented milk could alleviate DSS-induced IBD via inhibition of inflammatory response, promotion of the mucosal barrier reconstruction and regulation of the intestinal flora.

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