Campylobacter-derived ligands induce cytokine and chemokine expression in chicken macrophages and cecal tonsil mononuclear cells

2020 ◽  
Vol 246 ◽  
pp. 108732 ◽  
Author(s):  
Khaled Taha-Abdelaziz ◽  
Jake Astill ◽  
Bahram Shojadoost ◽  
Silvia Borrelli ◽  
Mario A Monteiro ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Chemokine Expression ◽  
Cecal Tonsil

scholarly journals Identification of IFITM1 and IFITM3 in Goose: Gene Structure, Expression Patterns, and Immune Reponses against Tembusu Virus Infection

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Anqi Wang ◽  
Lipei Sun ◽  
Mingshu Wang ◽  
Renyong Jia ◽  
Dekang Zhu ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Expression Patterns ◽  
Harderian Gland ◽  
Bursa Of Fabricius ◽  
Poly I:C ◽  
Peripheral Blood Mononuclear ◽  
Interferon Stimulated Genes ◽  
Cecal Tonsil ◽  
Tembusu Virus ◽  
First Time

As interferon-stimulated genes (ISGs), interferon-inducible transmembrane proteins 1 and 3 (IFITM1 and IFITM3) can effectively inhibit the replication of multiple viruses. Here, goose IFITM1 and IFITM3 were cloned and identified for the first time. The two proteins share the same topological structure and several important sites critical for the antiviral functions in other species are conserved in the goose. Goose IFITM1 and IFITM3 are most closely related to their respective orthologs in ducks; these proteins exhibited high mRNA transcript levels in immune-related tissues, including the thymus, bursa of Fabricius, and Harderian gland, compared to other tissues. Moreover, goose IFITM1 was highly constitutively expressed in gastrointestinal tract tissues, while goose IFITM3 was expressed in respiratory organs. Furthermore, goose IFITM3 was activated in goose peripheral blood mononuclear cells (PBMCs) infected with Tembusu virus (TMUV) or treated with Toll-like receptors (TLRs) agonists, while only the R848 and Poly (I:C) agonists induced significant upregulation of goose IFITM1. Furthermore, goose IFITM1 and IFITM3 were upregulated in the sampled tissues, to some extent, after TMUV infection. Notably, significant upregulation of goose IFITM1 and IFITM3 was detected in the cecum and cecal tonsil, where TMUV was primarily distributed. These data provide new insights into the immune effectors in geese and promote our understanding of the role of IFITM1 and IFITM3 in the defense against TMUV.

scholarly journals Probiotic Lactobacilli Limit Avian Influenza Virus Subtype H9N2 Replication in Chicken Cecal Tonsil Mononuclear Cells

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 605
Author(s):  
Nadiyah Alqazlan ◽  
Mohammadali Alizadeh ◽  
Nitish Boodhoo ◽  
Khaled Taha-Abdelaziz ◽  
Eva Nagy ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Immune System ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Mononuclear Cells ◽  
Host Immune System ◽  
Beneficial Effects ◽  
Cpg Oligodeoxynucleotides ◽  
Cecal Tonsil ◽  
Probiotic Lactobacilli

Low pathogenic avian influenza virus (LPAIV) H9N2 poses significant threat to animal and human health. The growing interest in beneficial effects of probiotic bacteria on host immune system has led to research efforts studying their interaction with cells of host immune system. However, the role of lactobacilli in inducing antiviral responses in lymphoid tissue cells requires further investigation. The objective of the present study was to examine the antiviral and immunostimulatory effects of lactobacilli bacteria on chicken cecal tonsils (CT) cells against H9N2 LPAIV. CT mononuclear cells were stimulated with probiotic Lactobacillus spp mixture either alone or in combination with a Toll-like receptor (TLR)21 ligand, CpG oligodeoxynucleotides (CpG). Pre-treatment of CT cells with probiotic lactobacilli, alone or in combination with CpG, significantly reduced H9N2 LPAIV replication. Furthermore, lactobacilli alone elicited cytokine expression, including IL-2, IFN-γ, IL-1β, IL-6, and IL-12, and IL-10, while when combined with CpG, a significantly higher expression of (interferon-stimulated gene (viperin)), IL-12, IL-6, CXCLi2, and IL-1β was observed. However, none of these treatments induced significant changes in nitric oxide production by CT cells. In conclusion, probiotic lactobacilli demonstrated a modulatory effect on CT cells, and this correlated with enhanced antiviral immunity and reduced H9N2 LPAIV viral replication.

scholarly journals Effects of Lactobacilli on Cytokine Expression by Chicken Spleen and Cecal Tonsil Cells

2010 ◽  
Vol 17 (9) ◽  
pp. 1337-1343 ◽  
Author(s):  
Jennifer T. Brisbin ◽  
Joshua Gong ◽  
Payvand Parvizi ◽  
Shayan Sharif
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Mononuclear Cells ◽  
Transforming Growth Factor ◽  
Lactobacillus Reuteri ◽  
Expression Profiles ◽  
Cytokine Gene Expression ◽  
Host Immune System ◽  
T Helper 1 ◽  
Cecal Tonsil

ABSTRACT Lactobacillus acidophilus, Lactobacillus reuteri, and Lactobacillus salivarius are all normal residents of the chicken gastrointestinal tract. Given the interest in using probiotic bacteria in chicken production and the important role of the microbiota in the development and regulation of the host immune system, the objective of the current study was to examine the differential effects of these bacteria on cytokine gene expression profiles of lymphoid tissue cells. Mononuclear cells isolated from cecal tonsils and spleens of chickens were cocultured with one of the three live bacteria, and gene expression was analyzed via real-time quantitative PCR. All three lactobacilli induced significantly more interleukin 1β (IL-1β) expression in spleen cells than in cecal tonsil cells, indicating a more inflammatory response in the spleen than in cecal tonsils. In cecal tonsil cells, substantial differences were found among strains in the capacity to induce IL-12p40, IL-10, IL-18, transforming growth factor β4 (TGF-β4), and gamma interferon (IFN-γ). In conclusion, we demonstrated that L. acidophilus is more effective at inducing T-helper-1 cytokines while L. salivarius induces a more anti-inflammatory response.

Antihypertensive therapy induces compartment-specific chemokine expression and a Th1 immune response in the clipped kidney of Goldblatt hypertensive rats

2007 ◽  
Vol 292 (2) ◽  
pp. F876-F887 ◽  
Author(s):  
O. M. Steinmetz ◽  
S. Sadaghiani ◽  
U. Panzer ◽  
C. Krebs ◽  
C. Meyer-Schwesinger ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Mononuclear Cells ◽  
Cluster Formation ◽  
Cell Cluster ◽  
Matrix Metalloproteinase 2 ◽  
Hypertensive Rats ◽  
Plasminogen Activator Inhibitor 1 ◽  
Diffuse Infiltration ◽  
Chemokine Expression ◽  
Vasopeptidase Inhibitor

The present study examined the pathogenesis of interstitial inflammation and fibrosis in antihypertensively treated rats with two-kidney, one-clip hypertension. Hypertensive rats were randomized into four groups: no treatment and moderate, intermediate, and intensified lowering of blood pressure with increasing doses of a vasopeptidase inhibitor for 6 wk. The vasopeptidase inhibitor dose dependently lowered blood pressure. The tubulointerstitial damage was accompanied by a diffuse infiltration of mononuclear cells and circumscript mononuclear inflammatory cell cluster formation consisting mainly of T cells and to a lesser degree of macrophages and B cells. Real-time PCR analyses showed a dose-dependent induction of MCP-1 and the Th1-type chemokines IP10 and Mig as well as their receptor CXCR3 and the Th1 cytokine IFN-γ. In situ hybridization and laser microdissection revealed a strong expression of these Th1-associated transcripts in the clusters and, in the case of MCP-1, also diffusely in the interstitium. The inflammation was accompanied by the appearance of myofibroblasts and synthesis of the fibrogenic factor plasminogen activator inhibitor-1 as well as the collagenase matrix metalloproteinase-2, leading to collagen I upregulation and interstitial scarring. No inflammation or fibrosis was found in normotensive rats treated with the vasopeptidase inhibitor. The renal injury in the clipped kidney is accompanied by compartment-specific chemokine expression and cell cluster formation of Th1 specificity associated with upregulation of fibrogenic proteins and matrix metalloproteinases. These findings suggest that the Th1 chemokines IP10 and Mig as well as their receptor CXCR3 are potential targets for therapeutic interventions in ischemic nephropathy.

scholarly journals Avian Macrophage Responses to Virulent and Avirulent Clostridium perfringens

Pathogens ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 100
Author(s):  
Raveendra R. Kulkarni ◽  
Carissa Gaghan ◽  
Javid Mohammed
Keyword(s):  
Nitric Oxide ◽  
Clostridium Perfringens ◽  
Mononuclear Cells ◽  
Surface Expression ◽  
No Production ◽  
Necrotic Enteritis ◽  
Whole Cells ◽  
Mhc Ii ◽  
Cecal Tonsil ◽  
Secretory Products

The present study evaluated the avian macrophage responses against Clostridium perfringens that varied in their ability to cause necrotic enteritis in chickens. Strains CP5 (avirulent-netB+), CP1 (virulent-netB+), and CP26 (highly virulent-netB+tpeL+) were used to evaluate their effect on macrophages (MQ-NCSU cells) and primary splenic and cecal tonsil mononuclear cells. The bacilli (whole cells) or their secretory products from all three strains induced a significant increase in the macrophage transcription of Toll-like receptor (TLR)21, TLR2, interleukin (IL)-1β, inducible nitric oxide synthase (iNOS), and CD80 genes as well as their nitric oxide (NO) production and major histocompatibility complex (MHC)-II surface expression compared to an unstimulated control. The CP1 and CP26-induced expression of interferon (IFN)γ, IL-6, CD40 genes, MHC-II upregulation, and NO production was significantly higher than that of CP5 and control groups. Furthermore, splenocytes and cecal tonsillocytes stimulated with bacilli or secretory products from all the strains showed a significant increase in the frequency of macrophages, their surface expression of MHC-II and NO production, while CP26-induced responses were significantly higher for the rest of the groups. In summary, macrophage interaction with C. perfringens can lead to cellular activation and, the ability of this pathogen to induce macrophage responses may depend on its level of virulence.

Atorvastatin reduces NF-κB activation and chemokine expression in vascular smooth muscle cells and mononuclear cells

1999 ◽  
Vol 147 (2) ◽  
pp. 253-261 ◽  
Author(s):  
Mónica Ortego ◽  
Carmen Bustos ◽  
Miguel A Hernández-Presa ◽  
José Tuñón ◽  
Cristina Dı́az ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Mononuclear Cells ◽  
Muscle Cells ◽  
Chemokine Expression

Ultrastructural diagnosis of plasmacytoma on a fine-needle aspirate

1991 ◽  
Vol 49 ◽  
pp. 102-103
Author(s):  
S. Siew ◽  
W. deMendonca-Calaca
Keyword(s):  
Mononuclear Cells ◽  
Fibrous Tissue ◽  
Needle Aspiration ◽  
Focal Lesions ◽  
Diffuse Infiltration ◽  
Fine Needle ◽  
Cytoplasmic Vacuolation ◽  
Chest Mass ◽  
Mitotic Figures ◽  
Microscopy Examination

A 36 year old man presented with a mass in the chest and multiple “hot” focal lesions were identified on bone scan. Fine needle aspiration was performed of the chest mass. Routine histology showed the presence of some bundles of dense fibrous tissue and a diffuse infiltration of mononuclear cells, which varied in size and nucleo-cytoplasmic ratio. The smaller cells had eccentric hyperchromatic nuclei. Nucleoli were noted in the larger cells. There was well marked cytoplasmic vacuolation of some of the cells. Mitosis was present. A small fragment of tissue was received for electron microscopy. Examination of 1 μm sections showed trabeculae of medium-large polygonal cells with eccentric nuclei and occasional nucleoli. Some irregularly shaped cells had well marked cytoplasmic vacuolation. Mitotic figures were present.

Sign in / Sign up

Export Citation Format

Share Document