Screening of acute and sub-chronic dermal toxicity of Calendula officinalis L essential oil

2018 ◽  
Vol 98 ◽  
pp. 184-189 ◽  
Author(s):  
Arun K. Mishra ◽  
Amrita Mishra ◽  
Pragya ◽  
Pronobesh Chattopadhyay
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Romuald Mordalski ◽  
Waldemar Buchwald ◽  
Elżbieta Bilińska ◽  
Hanna Zalińska ◽  
Wojciech A. Kucharski

Introduction. Marigold (Calendula officinalis L.) is an annual plants belongs to Asteraceae family. The species is widespread in the world and have a long tradition of medicinal use with multidirectional action. Aim. The field experiments were carried out to assess the impact of genetic (cultivar) and environmental factors on yielding variability and biosynthesis of essential oil in conditions of conventional cultivation of marigold after using different methods of plantation weeding. Material and methods. The research material were two cultivars of marigold such as Orange King and Indian Prince. The following activity were tested: 1) spraying of Fusilade Forte 150 EC (1 l • ha-1), 2) spraying Fusilade Forte 150 EC (1 l • ha-1) + manual weeding, 3) manual weeding, 4) no weeding – control. The scope of the research included assessment of marigold yield (inflorescence) and determination of the essential oil contents in dry mass of raw material. Results. Regardless of the cultivar, a non-chemical methods (mechanical weeding in interrows and manual in rows) gave the highest efficiency of weed control. Similar results observed after using chemical weeding with manual weeding. In these cases the yields were at least 2.5 times higher than obtained from the control. The total yield of dry mass of inflorescence for the above methods was 6.35 and 5.53 q • ha-1 for Orange King cultivars, slightly lower result obtained for cultivar of Indian Prince (4.81 and 4.12 q • ha-1). It was also shown that the content of essential oil in the raw material ranged from 0.25 to 0.37% and was independent from the cultivar. Conclusions. The Orange King cultivar of marigold yielded better than Indian Prince in analogous methods of plantation weeding. The highest effectiveness of weed plantation was found using a non-chemical method (mechanical weeding in interrows and manual weeding in rows) and using chemical weeding with manual weeding. It was shown that the content of essential oil in the inflorescences of both examined cultivars was similar.


2008 ◽  
Vol 39 (1) ◽  
pp. 61-63 ◽  
Author(s):  
Zilda Cristiane Gazim ◽  
Claudia Moraes Rezende ◽  
Sandra Regina Fraga ◽  
Terezinha Inez Estivaleti Svidzinski ◽  
Diógenes Aparicio Garcia Cortez

2016 ◽  
Vol 11 (8) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Ain Raal ◽  
Anne Orav ◽  
Julia Nesterovitsch ◽  
Kerttu Maidla

Pot Marigold (Calendula officinalis L.) is an annual ornamental plant with yellow to orange flowers which belongs the the Asteraceae family, its flowers are used in the pharmaceutical, cosmetic and food industries. The commercial samples of seeds (n=24) of C officinalis were cultivated in home gardens in Estonia. Total carotenoid contents, determined spectrophotometrically (λ=455 nm) in the inflorescences, varied from 0.7 to 2.7%, the cultivar with the highest carotenoid content was ‘Dracon’. The total content of flavonoids, determined spectrophotometrically (λ=370 nm), varied from 0.8% to 1.7%. The best cultivar contained almost maximum amount of total carotenoids (2.5%) and flavonoids (1.7%) was ‘Radio”. The oil yields of eight cultivars of C officinalis obtained were from 0.10 to 0.43%, the highest yield (0.43%) of the oil was obtained from the cultivar ‘Double Ball’. A total 70 compounds were identified and analysed by GC-MS in the essential oil of C officinalis, representing over 95% from total oil. The major components of the oils (>5%) of C. officinalis cultivars were α-cadinole (18.4-32.0%), γ-cadinene (9.7-18.9%), viridiflorol (2.0-10.0%), τ-cadinole (4.7-7.9%), δ-cadinole (3.3-5.6%), and γ-muurolene (0.8-5.1%). There is no clear relation between the colour of the inflorescences of C. officinalis and the total content of flavonoids and carotenoids. The chemotypes of essential oils of C. officinalis were not found among the cultivars studied.


2016 ◽  
pp. 57-60
Author(s):  
Judit Lelesz ◽  
Éva Nagy ◽  
József Csajbók

During our research we investigated the marigold's (Calendula officinalis L.) nutrient requirements with different fertilization setting in small-plot trial. We measured SPME (Solid phase microextraction) and GC-MS (gas chromatograph-mass spectrometer) we examined the effects of the different fertilization settings for the herb's main active ingredients of essential oil's percentage. Based on the results, it was concluded, the essential oil agents' percentage breakdowns significantly depending on the cropping technologies. Besides that it is possible, based on Pearson's correlation test the marigold essential oil agents relationship can also be a major factor.


2007 ◽  
Vol 72 (4) ◽  
pp. 407-413 ◽  
Author(s):  
Lidija Petrovic ◽  
Zika Lepojevic ◽  
Verica Sovilj ◽  
Dusan Adamovic ◽  
Vele Tesevic

Essential oil content (0.05 %) of marigold (Calendula officinalis L.) was determined using the official steam distillation procedure. High-pressure CO2 extraction of plant material under supercritial (100, 200 and 300 bar and 40?C) and subcritical (60, 90 and 120 bar and 15?C) conditions for 3 h was investigated. It was found that the increase in the pressure promoted an increase in the yield. The essential oil contents obtained from the investigated CO2 extracts by steam distillation were significantly higher (1.52-2.70 times) and increased with pressure. Major constituents of the oil, identified using GC-MS and GC-FID, were ?-cadinol (26.54 %), T-cadinol and T-muurolol (9.80 %), ?-cadinene (2.99 %), hexadecanoic acid (2.95 %), and ledane (2.45 %). In addition, the essential oils of the CO2 extracts contained ?-cadinene (6.50-19.87 % under supercritical and 16.09-19.41 % under subcritical conditions), which was not found in the essential oil obtained from the plant by steam distillation. The extraction kinetics was investigated at 200 bar and 40 ?C. The total extract obtained after 10 h of extraction was 6.54 % and essential oil content in it, refering to plant material, was 0.209 %, which is 4.16 time more than the one determined by the standard steam distillation procedure.


Author(s):  
B. G. Tsugkiev ◽  
M. K. Ailyarova ◽  
E. I. Rekhviashvili ◽  
S. A. Grevtsova ◽  
M. Yu. Kabulova

The starting material for the production of natural dyes are mainly plants. Coloring substances are usually obtained from various parts of plants: flowers, berries, roots, leaves containing pigments, the color of which is due to the presence of compounds such as anthocyanins, carotenoids, chlorophyll, flavonoids, etc. The most promising plant from the point of view of obtaining a yellow colorant is a medicinal calendula (Calendula officinalis L). Coloring pigment is mainly in the epidermis of the corollas of flowers and belongs to the group of carotenoid dyes, giving a yellow color. Its content in the flowers of calendula depends on the variety and growing conditions. Inflorescences of calendula officinalis contain carotenoids 2.8 %, the organic acid is 7.8 %, tannins to 0.02 %, essential oil of 0.03 %, mucus 4 %, ascorbic acid was 4.2 %. The chemical composition of calendula flowers, allows them to be used for dye. A method of extracting coloring substances from the inflorescences of calendula is developed.


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