Esophageal Cannula Dog, a Simple Mode of Preparation for Sham Feeding Experiments

1959 ◽  
Vol 37 (4) ◽  
pp. 460-462 ◽  
Author(s):  
Lars Olbe
1992 ◽  
Vol 262 (4) ◽  
pp. R554-R561 ◽  
Author(s):  
D. P. Yox ◽  
L. Brenner ◽  
R. C. Ritter

To test the possibility that endogenous cholecystokinin (CCK) participates in suppression of sham feeding by intraintestinal nutrient infusions, we examined the effect of CCK-receptor antagonists on the suppression of sham feeding by intraintestinally infused oleic acid, maltose or L-phenylalanine (L-Phe). In addition, we monitored amylase activity in the intestinal lumen during some sham feeding experiments and measured plasma CCK in parallel experiments using intestinally infused animals that were not feeding. Suppression of sham feeding by oleic acid or maltose was attenuated by CCK-receptor antagonists, while suppression of sham feeding by L-Phe was not. Oleate infusion increased plasma CCK concentration and luminal amylase activity. Oleate-induced increase in luminal amylase activity was attenuated by a CCK-receptor antagonist. Intraintestinal maltose or L-Phe did not increase plasma CCK concentration or luminal amylase activity, suggesting that they did not release endocrine CCK. These results suggest 1) that endogenous CCK mediates suppression of sham feeding by oleate and maltose but not by L-Phe and 2) that CCK participating in suppression of feeding by intestinal stimuli might not be of endocrine origin.


2005 ◽  
Vol 288 (6) ◽  
pp. R1695-R1706 ◽  
Author(s):  
Prasanth K. Chelikani ◽  
Alvin C. Haver ◽  
Roger D. Reidelberger

Glucagon-like peptide-1(7–36)-amide (GLP-1) is postulated to act as a hormonal signal from gut to brain to inhibit food intake and gastric emptying. A mixed-nutrient meal produces a 2 to 3-h increase in plasma GLP-1. We determined the effects of intravenous infusions of GLP-1 on food intake, sham feeding, and gastric emptying in rats to assess whether GLP-1 inhibits food intake, in part, by slowing gastric emptying. A 3-h intravenous infusion of GLP-1 (0.5–170 pmol·kg−1·min−1) at dark onset dose-dependently inhibited food intake in rats that were normally fed with a potency (mean effective dose) and efficacy (maximal % inhibition) of 23 pmol·kg−1·min−1 and 82%, respectively. Similar total doses of GLP-1 administered over a 15-min period were less potent and effective. In gastric emptying experiments, GLP-1 (1.7–50 pmol·kg−1·min−1) dose-dependently inhibited gastric emptying of saline and ingested chow with potencies of 18 and 6 pmol·kg−1·min−1 and maximal inhibitions of 74 and 83%, respectively. In sham-feeding experiments, GLP-1 (5–50 pmol·kg−1·min−1) dose-dependently reduced 15% aqueous sucrose intake in a similar manner when gastric cannulas were closed (real feeding) and open (sham feeding). These results demonstrate that intravenous infusions of GLP-1 dose-dependently inhibit food intake, sham feeding, and gastric emptying with a similar potency and efficacy. Thus GLP-1 may inhibit food intake in part by reducing gastric emptying, yet can also inhibit food intake independently of its action to reduce gastric emptying. It remains to be determined whether intravenous doses of GLP-1 that reproduce postprandial increases in plasma GLP-1 are sufficient to inhibit food intake and gastric emptying.


1993 ◽  
Vol 69 (1) ◽  
pp. 37-47 ◽  
Author(s):  
Pablo E. Colucci ◽  
W. Larry Grovum

1. Sheep with oesophageal fistulas were used in sham-feeding experiments to assess how sham intakes were affected by (a) physical form of straw (finely and coarsely ground; ground and pelleted), (b) type of food (straw pellets v. lucerne (Medicago sativa) hay pellets) and (c) additions of monosodium glutamate (MSG) with or without NaCl to the various straw diets. Normal animals were also fed on diets with and without MSG. Sham intakes of fine-ground loose straw (25 g/30 min) were markedly less (P = 0.002) than those of ground and pelleted straw (711 g/30 min). However, MSG at 5–40 g/kg fine and coarse ground straw increased sham intakes by 146 (P = 0.04) and 164% (P = 0.01) respectively. These findings indicated that the intakes of poor-quality diets can be increased by compacting them or by improving their palatability with MSG, or both. Sham intakes of straw pellets in two experiments were 32 (P = 0.02) and 45% (P = 0.008) of those of lucerne pellets (436 v. 1366 and 737 v. 1640 g/30 min). However, MSG at 20 g/kg straw pellets increased sham intakes from 674 to 1100 g/30 min (P = 0.05). When the MSG was mixed with NaCl (20 g/kg), the intakes of straw pellets were increased from 1089 to 1512 g/30 min (P = 0.02). Thus, the addition of MSG with or without NaCl increased the intakes of straw pellets. The highest intakes of the straw pellets treated with MSG were similar to those for lucerne pellets. When MSG-treated ammoniated barley straw (10 g/kg) was fed to normal sheep, the MSG increased DM intakes by 10 % (719–789 g/d; P = 0.04). MSG sprayed onto grass hay (10 g/kg) did not, however, affect daily DM intakes by these sheep. In general, the findings indicate that the intake of straw by ruminants may be increased by compressing it to form pellets or cubes and by adding MSG.


1950 ◽  
Vol 16 (1) ◽  
pp. 104-116 ◽  
Author(s):  
Henry D. Janowitz ◽  
Franklin Hollander ◽  
David Orringer ◽  
Milton H. Levy ◽  
Asher Winkelstein ◽  
...  

1965 ◽  
Vol 20 (6) ◽  
pp. 1185-1197 ◽  
Author(s):  
Michel Morineau
Keyword(s):  

Après avoir sacrifié longtemps aux dieux virils (Mars, Apollon, Mercure) Clio se tourne de plus en plus vers les autels des déesses-mères : Cérès, Vénus, Genitrix… Cette image, qui nous est inspirée par le titre d'un des ouvrages utilisés ci-dessous, traduit, à sa manière, l'évolution de la recherche historique, depuis sa seconde naissance au XIXe siècle. Est-ce une simple mode, comme, naguère, l'engouement pour les livres de raison ? Non, sans doute. Histoire agraire et démographie constituent le tuf de l'histoire tout court. Elles appellent d'ailleurs, à cause de leur caractère total, la coordination des efforts et l'établissement de plans d'enquête rigoureux. A un niveau plus modeste, ce que nous essayerons ici, c'est de confronter un certain nombre de travaux récents hollandais, belges et français, tant du point de vue de leurs méthodes que du point de vue de leurs résultats.


1990 ◽  
Vol 63 (2) ◽  
pp. 305-318 ◽  
Author(s):  
Richard R. Carter ◽  
O. Brian Allen ◽  
W. Larry Grovum

Total and unilateral parotid saliva production during eating were measured in response to offering sheep a fixed amount of lucerne (Medicago sativa) hay as one, two, four or eight meals. Total saliva measurements were obtained using sham-fed oesophageal-fistulated sheep. Unilateral parotid saliva was collected from sheep fitted with reversible re-entrant cannulas. Dry matter intakes and eating times were measured for each meal but were not constrained to particular values. Total and unilateral parotid saliva production during eating increased linearly with the log of the number of meals (p = 0.0001). The amounts corresponding to one, two, four and eight meals of lucerne hay were 1553, 1737, 1851 and 2087 ml during total collections and 209, 248, 307 and 352 ml during unilateral parotid collections. The time-period spent eating and the amount of food consumed both increased as meal number increased. Total saliva collections when lucerne hay was sham-fed as one, two, four or eight meals were associated with eating times of 56.9, 57.4, 70.8 and 86.0 min and intakes of 562, 622, 629 and 638 g dry matter respectively. For unilateral parotid collections, eating times and dry matter intakes were 64.2, 71.3, 78.0, 82.1 min and 515, 579, 614 and 627 g for one, two, four and eight meals of lucerne hay respectively. The saliva production response appeared to be determined through the effects of the time-period spent eating and amounts consumed, but other undetermined effects of feeding frequency contributed to the response. The importance of meal duration on total saliva production was assessed by sham-feeding of 800 g lucerne as stem, leaf, hay, chopped hay or ground and pelleted hay. Increasing meal duration by feeding with stems resulted in the production of 1808 ml saliva, whereas the rapid consumption of pellets resulted in only 442 ml being produced.


1995 ◽  
Vol 69 (1) ◽  
pp. 69-75 ◽  
Author(s):  
T. Scholz ◽  
M.C.F. Pech-Ek ◽  
R. Rodriguez-Canul

AbstractField study on the biology of Crassicutis cichlasomae Manter, 1936 (Digenea: Homalometridae) was carried out in a small swamp in a limestone factory near Mérida, Yucatán, Mexico. Aquatic snails, Littorina (Littoridinopsis) angulifera, harbouring C. cichlasomae rediae, cercariae and metacercariae, served both as the first and second intermediate hosts. Feeding experiments confirmed the conspecificity of metacercariae from naturally infected snails with adults from naturally infected fish. Gravid C. cichlasomae worms were obtained from experimentally infected fish 19 days post exposure at 22–24°C. Examination of fish from the swamp in Mitza and other localities in the Yucatan Peninsula showed that the cichlids Cichlasoma urophthalmus and C. meeki were definitive hosts of C. cichlasomae. There was no pronounced preference of C. cichlasomae adults for the site of their location in the intestine of the definitive host; a slightly higher proportion (41%) of worms was only found in the anterior third of the gut. The time of miracidium development varied from 18.5 to 27.5 days; different temperature (20.1–35.7°C) or light/darkness regimes influenced only slightly the rate of embryonic development, with shorter development times at higher temperature (34.8–35.7°C) and constant darkness and/or light. With the exception of the sporocyst, all developmental stages are described and figured.


2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Ajeet Kumar Mohanty ◽  
Charles de Souza ◽  
Deepika Harjai ◽  
Prathamesh Ghavanalkar ◽  
Mezia Fernandes ◽  
...  

Abstract Background Efforts to study the biology of Plasmodium vivax liver stages, particularly the latent hypnozoites, have been hampered by the limited availability of P. vivax sporozoites. Anopheles stephensi is a major urban malaria vector in Goa and elsewhere in South Asia. Using P. vivax patient blood samples, a series of standard membrane-feeding experiments were performed with An. stephensi under the US NIH International Center of Excellence for Malaria Research (ICEMR) for Malaria Evolution in South Asia (MESA). The goal was to understand the dynamics of parasite development in mosquitoes as well as the production of P. vivax sporozoites. To obtain a robust supply of P. vivax sporozoites, mosquito-rearing and mosquito membrane-feeding techniques were optimized, which are described here. Methods Membrane-feeding experiments were conducted using both wild and laboratory-colonized An. stephensi mosquitoes and patient-derived P. vivax collected at the Goa Medical College and Hospital. Parasite development to midgut oocysts and salivary gland sporozoites was assessed on days 7 and 14 post-feeding, respectively. The optimal conditions for mosquito rearing and feeding were evaluated to produce high-quality mosquitoes and to yield a high sporozoite rate, respectively. Results Laboratory-colonized mosquitoes could be starved for a shorter time before successful blood feeding compared with wild-caught mosquitoes. Optimizing the mosquito-rearing methods significantly increased mosquito survival. For mosquito feeding, replacing patient plasma with naïve serum increased sporozoite production > two-fold. With these changes, the sporozoite infection rate was high (> 85%) and resulted in an average of ~ 22,000 sporozoites per mosquito. Some mosquitoes reached up to 73,000 sporozoites. Sporozoite production could not be predicted from gametocyte density but could be predicted by measuring oocyst infection and oocyst load. Conclusions Optimized conditions for the production of high-quality P. vivax sporozoite-infected An. stephensi were established at a field site in South West India. This report describes techniques for producing a ready resource of P. vivax sporozoites. The improved protocols can help in future research on the biology of P. vivax liver stages, including hypnozoites, in India, as well as the development of anti-relapse interventions for vivax malaria.


Sign in / Sign up

Export Citation Format

Share Document