Complement activation in EDTA blood/plasma samples may be caused by coagulation proteases

1997 ◽  
pp. 363-369
Author(s):  
Philippe H. Pfeifer ◽  
Tony E. Hugli ◽  
Earl W. Davie ◽  
Kazuo Fujikawa
Keyword(s):  
Blood Plasma ◽  
Complement Activation ◽  
Plasma Samples ◽  
Edta Blood

scholarly journals Alterations in lipid profile upon uterine fibroids and its recurrence

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Narine M. Tonoyan ◽  
Vitaliy V. Chagovets ◽  
Natalia L. Starodubtseva ◽  
Alisa O. Tokareva ◽  
Konstantin Chingin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Blood Plasma ◽  
Lipid Profile ◽  
Plasma Levels ◽  
Uterine Fibroids ◽  
Control Group ◽  
Cholesterol Esters ◽  
Plasma Samples ◽  
Diagnostic Models ◽  
First Time

AbstractUterine fibroids (UF) is the most common (about 70% cases) type of gynecological disease, with the recurrence rate varying from 11 to 40%. Because UF has no distinct symptomatology and is often asymptomatic, the specific and sensitive diagnosis of UF as well as the assessment for the probability of UF recurrence pose considerable challenge. The aim of this study was to characterize alterations in the lipid profile of tissues associated with the first-time diagnosed UF and recurrent uterine fibroids (RUF) and to explore the potential of mass spectrometry (MS) lipidomics analysis of blood plasma samples for the sensitive and specific determination of UF and RUF with low invasiveness of analysis. MS analysis of lipid levels in the myometrium tissues, fibroids tissues and blood plasma samples was carried out on 66 patients, including 35 patients with first-time diagnosed UF and 31 patients with RUF. The control group consisted of 15 patients who underwent surgical treatment for the intrauterine septum. Fibroids and myometrium tissue samples were analyzed using direct MS approach. Blood plasma samples were analyzed using high performance liquid chromatography hyphened with mass spectrometry (HPLC/MS). MS data were processed by discriminant analysis with projection into latent structures (OPLS-DA). Significant differences were found between the first-time UF, RUF and control group in the levels of lipids involved in the metabolism of glycerophospholipids, sphingolipids, lipids with an ether bond, triglycerides and fatty acids. Significant differences between the control group and the groups with UF and RUF were found in the blood plasma levels of cholesterol esters, triacylglycerols, (lyso) phosphatidylcholines and sphingomyelins. Significant differences between the UF and RUF groups were found in the blood plasma levels of cholesterol esters, phosphotidylcholines, sphingomyelins and triacylglycerols. Diagnostic models based on the selected differential lipids using logistic regression showed sensitivity and specificity of 88% and 86% for the diagnosis of first-time UF and 95% and 79% for RUF, accordingly. This study confirms the involvement of lipids in the pathogenesis of uterine fibroids. A diagnostically significant panel of differential lipid species has been identified for the diagnosis of UF and RUF by low-invasive blood plasma analysis. The developed diagnostic models demonstrated high potential for clinical use and further research in this direction.

The Complex Biochemical Study of the Blood Plasma, Obtained by Manual Plasmapheresis

2016 ◽  
pp. 155-157
Author(s):  
Andrii Korzh
Keyword(s):  
Blood Plasma ◽  
Biochemical Parameters ◽  
Biochemical Study ◽  
Plasma Samples ◽  
Fluorometric Method ◽  
Plasma Donation ◽  
Individual Index ◽  
First Time ◽  
Age And Sex ◽  
Donor Plasma

The plasma samples of 34 primary donors (22 men and 12 women) for the first time given the plasma by automated plasmapheresis (control surveillance), and 54 active donors of blood plasma (40 men and 14 women) being donors with non-less 14 days interval between donations, have been examined. The active male donors’ plasma averaged at 18,63±1,71 with individual index fluctuations from 2 to 78, female donors’ – 14,09±1,95 with individual index fluctuations from 2 to 45. The method of plasma obtaining is a manual plasmapheresis method. The surveyed groups were homogeneous for age and sex. Hematologic and biochemical parameters of all those persons have been examined and, basing on the conclusion of the professionals, everyone was admitted to the plasma donation. The content of middle mass molecules in plasma were determined by method of N.I. Gabrieljan, V.I. Lipatovoj (1984). The content of biogenic amines free fractions in plasma were determined by fluorometric method of B.V. Mikhailichenko, S.V. Vydyborets (1999). Analysis of the results showed that in the donor plasma samples obtained by manual plasmapheresis level of middle mass molecules, histamine, serotonin is significantly higher. The significance of obtained results has also been discussed.

scholarly journals Low-Bias RNA Sequencing of the HIV-2 Genome from Blood Plasma

2018 ◽  
Vol 93 (1) ◽  
Author(s):  
Katherine L. James ◽  
Thushan I. de Silva ◽  
Katherine Brown ◽  
Hilton Whittle ◽  
Stephen Taylor ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Blood Plasma ◽  
De Novo ◽  
A Priori ◽  
Pcr Amplification ◽  
Hiv Vaccine ◽  
Whole Genome ◽  
Plasma Samples ◽  
Target Enrichment ◽  
Rna Seq

ABSTRACTAccurate determination of the genetic diversity present in the HIV quasispecies is critical for the development of a preventative vaccine: in particular, little is known about viral genetic diversity for the second type of HIV, HIV-2. A better understanding of HIV-2 biology is relevant to the HIV vaccine field because a substantial proportion of infected people experience long-term viral control, and prior HIV-2 infection has been associated with slower HIV-1 disease progression in coinfected subjects. The majority of traditional and next-generation sequencing methods have relied on target amplification prior to sequencing, introducing biases that may obscure the true signals of diversity in the viral population. Additionally, target enrichment through PCR requiresa priorisequence knowledge, which is lacking for HIV-2. Therefore, a target enrichment free method of library preparation would be valuable for the field. We applied an RNA shotgun sequencing (RNA-Seq) method without PCR amplification to cultured viral stocks and patient plasma samples from HIV-2-infected individuals. Libraries generated from total plasma RNA were analyzed with a two-step pipeline: (i)de novogenome assembly, followed by (ii) read remapping. By this approach, whole-genome sequences were generated with a 28× to 67× mean depth of coverage. Assembled reads showed a low level of GC bias, and comparison of the genome diversities at the intrahost level showed low diversity in the accessory genevpxin all patients. Our study demonstrates that RNA-Seq is a feasible full-genomede novosequencing method for blood plasma samples collected from HIV-2-infected individuals.IMPORTANCEAn accurate picture of viral genetic diversity is critical for the development of a globally effective HIV vaccine. However, sequencing strategies are often complicated by target enrichment prior to sequencing, introducing biases that can distort variant frequencies, which are not easily corrected for in downstream analyses. Additionally, detaileda priorisequence knowledge is needed to inform robust primer design when employing PCR amplification, a factor that is often lacking when working with tropical diseases localized in developing countries. Previous work has demonstrated that direct RNA shotgun sequencing (RNA-Seq) can be used to circumvent these issues for hepatitis C virus (HCV) and norovirus. We applied RNA-Seq to total RNA extracted from HIV-2 blood plasma samples, demonstrating the applicability of this technique to HIV-2 and allowing us to generate a dynamic picture of genetic diversity over the whole genome of HIV-2 in the context of low-bias sequencing.

scholarly journals Monitoring Radiotherapeutic Response in Prostate Cancer Patients Using High Throughput FTIR Spectroscopy of Liquid Biopsies

Cancers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 925 ◽  
Author(s):  
Dinesh K.R. Medipally ◽  
Thi Nguyet Que Nguyen ◽  
Jane Bryant ◽  
Valérie Untereiner ◽  
Ganesh D. Sockalingum ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Blood Plasma ◽  
Ftir Spectroscopy ◽  
Cancer Patients ◽  
Radiation Toxicity ◽  
Plasma Samples ◽  
Analysis Model ◽  
Liquid Biopsies ◽  
Time Points ◽  
Wide Range

Radiation therapy (RT) is used to treat approximately 50% of all cancer patients. However, RT causes a wide range of adverse late effects that can affect a patient’s quality of life. There are currently no predictive assays in clinical use to identify patients at risk of normal tissue radiation toxicity. This study aimed to investigate the potential of Fourier transform infrared (FTIR) spectroscopy for monitoring radiotherapeutic response. Blood plasma was acquired from 53 prostate cancer patients at five different time points: prior to treatment, after hormone treatment, at the end of radiotherapy, two months post radiotherapy and eight months post radiotherapy. FTIR spectra were recorded from plasma samples at all time points and the data was analysed using MATLAB software. Discrimination was observed between spectra recorded at baseline versus follow up time points, as well as between spectra from patients showing minimal and severe acute and late toxicity using principal component analysis. A partial least squares discriminant analysis model achieved sensitivity and specificity rates ranging from 80% to 99%. This technology may have potential to monitor radiotherapeutic response in prostate cancer patients using non-invasive blood plasma samples and could lead to individualised patient radiotherapy.

Diagnosis of Gulf War Illness Using Laser-Induced Spectra Acquired from Blood Samples

2021 ◽  
pp. 000370282110420
Author(s):  
Rosalba Gaudiuso ◽  
Sirui Chen ◽  
Efi Kokkotou ◽  
Lisa Conboy ◽  
Eric Jacobson ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Correct Diagnosis ◽  
Difference Spectrum ◽  
Gulf War ◽  
Classification Model ◽  
Control Group ◽  
Plasma Samples ◽  
Gulf War Illness ◽  
Breakdown Spectroscopy ◽  
The Difference

Gulf War illness (GWI) is a chronic illness with no known validated biomarkers that affects the lives of hundreds of thousands of people. As a result, there is an urgent need for the development of an untargeted and unbiased method to distinguish GWI patients from non-GWI patients. We report on the application of laser-induced breakdown spectroscopy (LIBS) to distinguish blood plasma samples from a group of subjects with GWI and from subjects with chronic low back pain as controls. We initially obtained LIBS data from blood plasma samples of four GWI patients and four non-GWI patients. We used an analytical method based on taking the difference between a mean LIBS spectrum obtained with those of GWI patients from the mean LIBS spectrum of those of the control group, to generate a “difference” spectrum for our classification model. This model was cross-validated using different numbers of differential LIBS emission peaks. A subset of 17 of the 82 atomic and ionic transitions that provided 70% of correct diagnosis was selected test in a blinded fashion using 10 additional samples and was found to yield 90% classification accuracy, 100% sensitivity, and 83.3% specificity. Of the 17 atomic and ionic transitions, eight could be assigned unambiguously to species of Na, K, and Fe.

scholarly journals Human biomonitoring of per- and polyfluoroalkyl substances in German blood plasma samples from 1982 to 2019

2020 ◽  
Vol 145 ◽  
pp. 106123
Author(s):  
Bernd Göckener ◽  
Till Weber ◽  
Heinz Rüdel ◽  
Mark Bücking ◽  
Marike Kolossa-Gehring
Keyword(s):  
Blood Plasma ◽  
Human Biomonitoring ◽  
Plasma Samples ◽  
Polyfluoroalkyl Substances

Acid-base changes in the running greyhound: contributing variables

1992 ◽  
Vol 73 (6) ◽  
pp. 2297-2304 ◽  
Author(s):  
R. L. Pieschl ◽  
P. W. Toll ◽  
D. E. Leith ◽  
L. J. Peterson ◽  
M. R. Fedde
Keyword(s):  
Blood Plasma ◽  
Strong Ion Difference ◽  
Plasma Samples ◽  
Acid Base ◽  
Sprint Exercise ◽  
Arterial Blood ◽  
Base Analysis

To determine the factors responsible for changes in [H+] during and after sprint exercise in the racing greyhound, Stewart's quantitative acid-base analysis was applied to arterial blood plasma samples taken at rest, at 8-s intervals during exercise, and at various intervals up to 30 min after a 402-m spring (approximately 30 s) on the track. [Na+], [K+], [Cl-], [total Ca], [lactate], [albumin], [Pi], PCO2, and pH were measured, and the [H+] was calculated from Stewart's equations. This short sprint caused all measured variables to change significantly. Maximal changes were strong ion difference decreased from 36.7 meq/l at rest to 16.1 meq/l; [albumin] increased from 3.1 g/dl at rest to 3.7 g/dl; PCO2, after decreasing from 39.6 Torr at rest to 27.9 Torr immediately prerace, increased during exercise to 42.8 Torr and then again decreased to near 20 Torr during most of recovery; and [H+] rose from 36.6 neq/l at rest to a peak of 76.6 neq/l. The [H+] calculated using Stewart's analysis was not significantly different from that directly measured. In addition to the increase in lactate and the change in PCO2, changes in [albumin], [Na+], and [Cl-] also influenced [H+] during and after sprint exercise in the running greyhound.

Relation of concentration of growth hormone in blood plasma to growth rate and carcass characteristics in the pig

1975 ◽  
Vol 20 (1) ◽  
pp. 51-61 ◽  
Author(s):  
R. J. Chappel ◽  
A. C. Dunkin
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Blood Plasma ◽  
Relative Growth Rate ◽  
Correlation Coefficients ◽  
Carcass Characteristics ◽  
Plasma Samples ◽  
Hormone Concentration ◽  
Plasma Growth Hormone ◽  
Relative Growth

SUMMARY1. Pig growth hormone (PGH) concentrations were measured in plasma samples from piglets of 3–4 to 7–8 weeks of age.2. Plasma PGH concentrations decreased significantly with age. No difference was observed between the hormone concentrations of gilts and barrows.3. Correlation coefficients were calculated between mean plasma growth hormone concentration over a 4-week period and several carcass characteristics and measures of growth rate. Plasma PGH over this period showed significant negative correlations with several measures of carcass backfat thickness at bacon weight. In barrows only, PGH concentrations showed a significant direct correlation with relative growth rate from 3 to 7 weeks.

P1077PERSISTENCE OF CIRCULATING RESIDUAL HEPARIN IN ESRD PATIENTS UNDERGOING MAINTENANCE HEMODIALYSIS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Vinod Bansal ◽  
Melissa Mazariegos ◽  
Arjun Grewal ◽  
Jasdeep Bajwa ◽  
Emily Bontekoe ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Maintenance Hemodialysis ◽  
Dialysis Session ◽  
Plasma Samples ◽  
Standard Laboratory ◽  
Reference Standard ◽  
Laboratory Methods ◽  
Thrombotic Complications ◽  
Esrd Patients ◽  
Digestion Methods

Abstract Background and Aims ESRD patients who receive routine maintenance hemodialysis are administered with unfractionated heparin to prevent thrombotic complications. The hemostatic dysregulation along with detectable levels of circulating heparin may cause them to be in a hypocoagulable state. The purpose of this study is to determine the circulating levels of heparin in ESRD patients and its characterization using heparinase digestion methods. Method Blood plasma samples collected at routine pre-dialysis sessions from 95 ESRD patients undergoing maintenance hemodialysis were analyzed for the presence of residual heparin utilizing standard laboratory methods such as aPTT, Anti-Xa and Anti-IIa activities On a centrifugal analyzer (ACL-Elite: Instrumentation Laboratory, Bedford, MA). The levels of heparin were calculated in terms of units per ml relative to the USP reference standard. Heparinase digestion was used to confirm the presence of heparin. Results Blood plasma samples collected at routine pre-dialysis sessions from 95 ESRD patients undergoing maintenance hemodialysis were analyzed for the presence of residual heparin utilizing standard laboratory methods such as aPTT, Anti-Xa and Anti-IIa activities On a centrifugal analyzer (ACL-Elite: Instrumentation Laboratory, Bedford, MA). The levels of heparin were calculated in terms of units per ml relative to the USP reference standard. Heparinase digestion was used to confirm the presence of heparin. Conclusion The presence of residual heparin was demonstrated by both clot- based and amidolytic assays in the plasma samples collected from ESRD patients prior to their next-dialysis session. Since these samples were obtained 3 days following the last dialysis session the presence of significant levels of heparin was surprising. Upon heparinase treatment of these samples, the aPTT and the anti-Xa and IIa tests were restored to near normal levels. Our studies confirm the presence of residual heparin in pre-dialysis plasma samples obtained from ESRD patients. The Anti-IIa activity was greater pre-heparinase and it was not decreased to the same extent as Anti-Xa after heparinase digestion. These results suggest that heparin found in ESRD patient’s plasma is of high molecular weight origin with delayed clearance.

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