Nicorandil inhibits the release of TNFα from a lymphocyte cell line and peripheral blood lymphocytes

2003 ◽  
Vol 3 (12) ◽  
pp. 1581-1588 ◽  
Author(s):  
X.M. Wei ◽  
G.J. Heywood ◽  
N. Di Girolamo ◽  
P.S. Thomas
Keyword(s):  
Cell Line ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Lymphocyte Cell ◽  
Lymphocyte Cell Line

scholarly journals CYTOTOXIC EFFECTS OF ARIPIPRAZOLE ON MKN45 AND NIH3T3 CELL LINES AND GENOTOXIC EFFECTS ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES

2019 ◽  
Vol 56 (2) ◽  
pp. 155-159 ◽  
Author(s):  
Mohammad SHOKRZADEH ◽  
Abbas MOHAMMADPOUR ◽  
Mona MODANLOO ◽  
Melika HASSANI ◽  
Nasrin Ghassemi BARGHI ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Normal Cell ◽  
Micronucleus Assay ◽  
Peripheral Blood Lymphocytes ◽  
Nih3t3 Cell ◽  
Dopamine Antagonists ◽  
Blood Lymphocytes

ABSTRACT BACKGROUND: Gastric cancer is known as the fourth most common cancer. Current treatments for cancer have damaged the sensitive tissues of the healthy body, and in many cases, cancer will be recurrent. Therefore, need for treatments that are more effective is well felt. Researchers have recently shifted their attention towards antipsychotic dopamine antagonists to treat cancer. The anticancer activities of aripiprazole remain unknown. OBJECTIVE: This study aimed to evaluate the efficacy and safety of aripiprazole on gastric cancer and normal cell lines. METHODS: In this regard, the cytotoxicity and genotoxicity of aripiprazole were investigated in MKN45 and NIH3T3 cell lines by methyl tetrazolium assay and on peripheral blood lymphocytes by micronucleus assay. For this purpose, cells were cultured in 96 wells plate. Stock solutions of aripiprazole and cisplatin were prepared. After cell incubation with different concentrations of aripiprazole (1, 10, 25, 50, 100 and 200 μL), methyl tetrazolium solution was added. For micronucleus assay fresh blood was added to RPMI culture medium 1640 supplemented, and different concentrations of aripiprazole (50, 100 and 200 μL) were added. RESULTS: The finding of present study showed that the IC50 of aripiprazole in the cancer cell line (21.36 μg/mL) was lower than that in the normal cell line (54.17 μg/mL). Moreover, the micronucleus assay showed that the frequency of micronuclei of aripiprazole at concentrations below 200 μM was much less than cisplatin. CONCLUSION: Aripiprazole can be a good cytotoxic compound and good candidate for further studies of cancer therapy.

Cytotoxic, genotoxic and biochemical markers of insecticide toxicity evaluated in human peripheral blood lymphocytes and an HepG2 cell line

2016 ◽  
Vol 96 ◽  
pp. 90-106 ◽  
Author(s):  
Davor Želježić ◽  
Marin Mladinić ◽  
Suzana Žunec ◽  
Ana Lucić Vrdoljak ◽  
Vilena Kašuba ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Peripheral Blood ◽  
Biochemical Markers ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Hepg2 Cell Line ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Insecticide Toxicity

scholarly journals Quantitative PCR used to Assess HIV-1 Integration and 2-LTR Circle Formation in Human Macrophages, Peripheral Blood Lymphocytes and a CD4+ Cell Line

2010 ◽  
Vol 7 (1) ◽  
pp. 354 ◽  
Author(s):  
Brian Friedrich ◽  
Guangyu Li ◽  
Natallia Dziuba ◽  
Monique R Ferguson
Keyword(s):  
Cell Line ◽  
Quantitative Pcr ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Human Macrophages ◽  
Blood Lymphocytes ◽  
Circle Formation ◽  
Cd4 Cell ◽  
Hiv 1

scholarly journals Increase of protein synthesis by uridine supplement in lectin-stimulated peripheral blood lymphocytes and EB virus-transformed B cell line of hereditary orotic aciduria type I.

1987 ◽  
Vol 153 (3) ◽  
pp. 189-195 ◽  
Author(s):  
MAKOTO YAZAKI ◽  
KAZUKI OKAJIMA ◽  
MARIKO SUCHI ◽  
HIDEKO MORISHITA ◽  
YOSHIRO WADA
Keyword(s):  
Protein Synthesis ◽  
B Cell ◽  
Cell Line ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Type I ◽  
Blood Lymphocytes ◽  
Eb Virus ◽  
Orotic Aciduria

HLA-A*0201 Restricted Killing Activity of WT1-Specific CTL Generated From the Peripheral Blood Lymphocytes of Normal Healthy Donors.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 3026-3026
Author(s):  
Deepa Kolaseri Krishnadas ◽  
Mindy Stamer ◽  
Kim Dunham ◽  
Lei Bao ◽  
Kenneth Lucas
Keyword(s):  
T Cells ◽  
Cell Line ◽  
Cd8 T Cells ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Target Cells ◽  
Cd8 Cells ◽  
Blood Lymphocytes ◽  
Healthy Donors ◽  
Ifn Γ

Abstract Abstract 3026 Poster Board II-1002 The Wilms' tumor antigen (WT1) is over-expressed on several human leukemia and solid tumors, and thus is considered as a potential target for cancer immunotherapy. Combating leukemia by targeting WT1 expressing leukemic cells using in vitro generated WT1-specific CTL is one potential approach, but it is difficult to generate an immune response against WT1 due to low T cell precursor frequency in normal healthy individuals. Earlier studies have shown the generation of WT1-A*0201 peptide specific CTL from CD8+ T cells by cloning. Another study reported the production of IFN- γ by WT-1 specific CD8+ T cells. However, the cytolytic killing ability of these IFN- γ producing cells was not further characterized. Here, we demonstrate the generation of WT1-A*0201 specific CTL from the peripheral blood lymphocytes (PBL) of normal healthy donors using CD137 selection. The PBL were stimulated once with RMFPNAPYL (WT1-A*0201 peptide) pulsed autologous dendritic cells and twice with WT1-A*0201 peptide pulsed irradiated peripheral blood mononuclear cells (PBMC). Following three stimulations, the PBL were selected for CD137+ expression and rapidly expanded with OKT3 and IL-2. The WT1-A*0201 specific CTL showed killing of target cells and production of IFN-γ in an antigen-specific manner. The percent killing of WT1-A*0201 peptide pulsed T2 cells (TAP−, HLA- A2+) and autologous B blast (BB) were significantly higher when compared with their control targets. T2 cells and BB either pulsed with an irrelevant A*0201 peptide or un-pulsed served as the control. We have observed similar results with WT1-A*0201 specific CTL generated from normal donor CD8+ cells. However, the efficiency of WT1-A*0201 CTL generated from PBL to kill target cells and produce IFN- γ was higher than CTL from CD8+ cells. The CTL generated from PBL killed BA25, a WT1 expressing A2+ leukemia cell line but failed to kill Molt-4, a WT1 expressing A2− cell line, clearly indicating HLA-A2 restricted CTL activity. The specificity of the generated CTL were further confirmed by staining with WT1-HLA-A*0201 tetramer. The percentage of WT1-specific CD3+CD8+Tetramer+ cells either remained same or higher in CTL generated from PBL when compared with those generated from CD8+ cells. CD137 selection leads to the generation of significant number of CTL in a shorter time when compared to conventional cloning methods. In addition, generation of WT1-A*0201 specific CTL from PBL avoids CD8+ selection. Currently, we are aiming to generate WT1-specific CTL using an overlapping WT1 peptide-mix in order to widen our ability to treat patients with different HLA types. This study has implications for cellular immunotherapy in leukemia patients who relapse following allogeneic stem cell transplantation. Disclosures No relevant conflicts of interest to declare.

Hypoxia induces lymphocyte adhesion to human mesenchymal cells via an LFA-1-dependent mechanism

1993 ◽  
Vol 264 (3) ◽  
pp. C617-C624 ◽  
Author(s):  
I. Ginis ◽  
S. J. Mentzer ◽  
D. V. Faller
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Cell Line ◽  
Cell Lines ◽  
Muscle Cell ◽  
Mesenchymal Cells ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Lymphocyte Cell ◽  
Lymphocyte Cell Line

We and others have previously reported that mesenchymal cells, including endothelial and muscle cells, sense oxygen tension and respond in a specific way during exposure to hypoxic environment. We have examined the interactions of human muscle and endothelial cells, which have been exposed to hypoxic environments, with T and B lymphoid cell lines and peripheral blood lymphocytes (PBL), not subjected to hypoxia. The adhesion of B lymphocyte cell line (JY) and the adhesion of T lymphocyte cell line (Jurkat) to muscle cell monolayers that had been incubated at PO2 of 50 Torr for 3 h increased more than four- and twofold, respectively. Hypoxia appears to upregulate a saturable muscle cell-associated adhesion mechanism, which is capable of withstanding distraction forces greater than 45 g, and is inhibitable by LFA-1-specific monoclonal antibodies (MAbs). Hypoxia also induced a reciprocal decrease in lymphocyte-muscle cell adhesion mechanisms inhibitable by VCAM-1- or VLA-4-specific MAbs. Cultured human endothelial cells when subjected to hypoxic conditions also increased their adhesion for lymphoid cells and cell lines. This induction of adhesion could again be attenuated by anti-LFA-1, but not by anti-ICAM-1 MAb, suggesting that hypoxia activates an adhesion molecule on human mesenchymal cells that is likely to be a new ligand for LFA-1. This report is the first demonstration of a direct induction of cell adhesion mechanisms by hypoxic environments.

scholarly journals Comparison and Evaluation of Different Radiotherapy Techniques Using Biodosimetry Based on Cytogenetics

Cancers ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 146
Author(s):  
Aggeliki Nikolakopoulou ◽  
Vasiliki Peppa ◽  
Antigoni Alexiou ◽  
George Pissakas ◽  
Georgia Terzoudi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Line ◽  
Peripheral Blood ◽  
Absorbed Dose ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Significant Difference ◽  
Biological Dose ◽  
Radiotherapy Techniques ◽  
3D Crt

While rapid technological advances in radiotherapy techniques have led to a more precise delivery of radiation dose and to a decreased risk of side effects, there is still a need to evaluate the efficacy of the new techniques estimating the biological dose and to investigate the radiobiological impact of the protracted radiotherapy treatment duration. The aim of this study is to compare, at a cytogenetic level, advanced radiotherapy techniques VMAT and IMRT with the conventional 3D-CRT, using biological dosimetry. A dicentric biodosimetry assay based on the frequency of dicentrics chromosomes scored in peripheral blood lymphocytes from prostate cancer patients and PC3 human prostate cancer cell line was used. For each patient blood sample and each subpopulation of the cultured cell line, three different irradiations were performed using the 3D-CRT, IMRT, and VMAT technique. The absorbed dose was estimated with the biodosimetry method based on the induced dicentric chromosomes. The results showed a statistically significant underestimation of the biological absorbed dose of ~6% for the IMRT and VMAT compared to 3D-CRT irradiations for peripheral blood lymphocytes, whereas IMRT and VMAT results were comparable without a statistically significant difference, although slightly lower values were observed for VMAT compared to IMRT irradiation. Similar results were obtained using the PC3 cell line. The observed biological dose underestimation could be associated with the relative decreased dose rate and increase irradiation time met in modulated techniques compared to the conventional 3D-CRT irradiations.

Development of a fusion partner cell line for efficient production of human monoclonal antibodies from peripheral blood lymphocytes

2002 ◽  
Vol 11 (3) ◽  
pp. 85-96 ◽  
Author(s):  
Gary F. Kalantarov ◽  
Sergey A. Rudchenko ◽  
Leslie Lobel ◽  
Ilya Trakht
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Line ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Efficient Production ◽  
Fusion Partner ◽  
Human Monoclonal Antibodies ◽  
Blood Lymphocytes

Antigenotoxic potential ofGymnema montanumleaves on DNA damage in human peripheral blood lymphocytes and HL-60 cell line

2009 ◽  
pp. NA-NA ◽  
Author(s):  
K.M. Ramkumar ◽  
L. Sankar ◽  
C. Manjula ◽  
K. Krishnamurthi ◽  
S. Saravana Devi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Line ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes
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