OMEGA-3 POLY-UNSATURATED FATTY ACIDS INHIBIT OMEGA 6 POLY-UNSATURATED FATTY ACID DIRECTED METASTASIS TOWARDS HUMAN BONE MARROW STROMA

M. Brown; C. Hart; E. Gazi; N. Clarke

doi:10.1016/s1569-9056(06)60583-6

Potensi Pemberian Cod Liver Oil (CLO) pada Pakan Komersial Terhadap Jumlah Total Asam Lemak Omega 3 dan Omega 6 Di Daging Udang Galah (Macrobrachium Rosenbergii) [Potential Of Giving Cod Liver Oil (CLO) Commercial Feed On To The Total Amount of Omega 3 and Omega 6 Fatty Acid in Meat Giant Freshwater Prawn (Macrobrachium Rosenbergii)]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v9i1.7634 ◽

2017 ◽

Vol 9 (1) ◽

pp. 65

Author(s):

Hartik Hartik ◽

Boedi Setya Rahardja ◽

Agustono Agustono

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Unsaturated Fatty Acid ◽

Macrobrachium Rosenbergii ◽

Cod Liver Oil ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Commercial Feed ◽

Omega 6 ◽

Poly Unsaturated Fatty Acid

AbstrakAsam lemak omega 3 dan omega 6 adalah asam lemak tak jenuh yang termasuk kedalam golongan Poly Unsaturated Fatty Acid (PUFA) dan juga termasuk dalam kelompok asam lemak esensial. Asam lemak omega 3 dan omega 6 tidak dapat disintesis sendiri oleh tubuh, sehingga perlu adanya suplai asam lemak omega 3 dan omega 6 di makanan yang dikonsumsi. Fungsi dari asam lemak omega 3 dan omega 6 secara fisiologis yaitu sebagai sumber penting dalam menunjang pertumbuhan dan kelangsungan hidup krustacea. Penelitian ini bertujuan untuk mengetahui potensi pemberian Cod Liver Oil (CLO) pada pakan komersial terhadap jumlah total asam lemak omega 3 dan omega 6 di daging udang galah (Macrobrachium rosenbergii). Metode penelitian yang digunakan adalah percobaan dengan Rancangan Acak Lengkap sebagai rancangan percobaan. Perlakuan yang digunakan adalah kandungan Cod Liver Oil (CLO) yang berbeda, yaitu P0 (0%), P1 (3%), P2(6%), P3 (9%), P4(12%) masing-masing perlakuan diulang sebanyak 4 kali. Parameter utama yang diamati adalah jumlah total asam lemak omega 3 dan omega 6 di daging udang galah. Parameter penunjang yang diamati adalah parameter kualitas air. Analisis data menggunakan analisis of varian (ANOVA) dan untuk mengetahui perlakuan terbaik dilakukan uji jarak berganda Duncan. Hasil penelitian pemberian Cod Liver Oil (CLO) menunjukkan berbeda nyata (P<0,05) terhadap jumlah total asam lemak omega 3 tetapi tidak berbeda nyata (P>0,05) dengan jumlah total asam lemak omrga 6 di daging udang galah (Macrobrachium rosenbergii). Jumlah total asam lemak omega 3 pada perlakuan P0 (0%) berbeda nyata dengan perlakuan P2 (6%), P3 (9%) dan P4 (12%), tetapi tidak berbeda nyata dengan perlakuan P1. Jumlah total asam lemak omega 6 pada perlakuan P0 (0%) tidak berbeda nyata dengan perlakuan P1 (3%), P2 (6%), P3 (9%) dan P4 (12%). AbstractFatty acids omega 3 and omega 6 unsaturated fatty acids are included into class Poly Unsaturated Fatty Acid (PUFA) and is also included in a group of essential fatty acids.Fatty acids omega 3 and omega 6 can not be synthesized by the body, so it is necessary to supply fatty acids omega 3 and omega 6 in the food consumed. This study aims to determine the potential for the provision of Cod Liver Oil (CLO) on a commercial feed to the total amount of fatty acids omega 3 and omega 6 in the flesh of giant fresh water prawn (Macrobrachium rosenbergii). The method used is experiment with completely randomized design as the experimental design. The treatment used is the content of Cod Liver Oil (CLO) that is different, that P0 (0%), P1 (3%), P2 (6%), P3 (9%), P4 (12%) of each treatment was repeated 4 times. The main parameters measured were the number of total fatty acids omega 3 and omega 6 in meat prawns. Supporting parameters measured were water quality parameters. Analysis of data using analysis of variance (ANOVA) and to determine the best treatment Duncan's multiple range test. The results of the research granting Cod Liver Oil (CLO) showed significantly (P <0.05) of the total amount of omega 3 fatty acids but not significantly different (P> 0.05) with the total amount of fatty acids omrga 6 in the meat giant fresh water prawn (Macrobrachium rosenbergii). The total amount of omega 3 fatty acids in the treatment of P0 (0%) was significantly different from P2 treatment (6%), P3 (9%) and P4 (12%), but not significantly different from P1 treatment. The total amount of fatty acids in the treatment omega 6 P0 (0%) did not differ significantly with treatment P1 (3%), P2 (6%), P3 (9%) and P4 (12%).

EFFECT OF GINGER, LEMURU FISH OIL SAPONIFICATION AND OLIVE OIL ON COMPOSITION FATTY ACID OF BEEF

Jurnal Agroindustri ◽

10.31186/j.agroind.4.1.31-39 ◽

2014 ◽

Vol 4 (1) ◽

pp. 31-39

Author(s):

Siwitri Kadarsih

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fish Oil ◽

Olive Oil ◽

Rice Bran ◽

Dry Matter ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Omega 3 ◽

Omega 6

The objective was to get beef that contain unsaturated fatty acids (especially omega 3 and 6), so as to improve intelligence, physical health for those who consume. The study design using CRD with 3 treatments, each treatment used 4 Bali cattle aged approximately 1.5 years. Observations were made 8 weeks. Pasta mixed with ginger provided konsentrat. P1 (control); P2 (6% saponification lemuru fish oil, olive oil 1%; rice bran: 37.30%; corn: 62.70%; KLK: 7%, ginger paste: 100 g); P3 (lemuru fish oil saponification 8%, 2% olive oil; rice bran; 37.30; corn: 62.70%; KLK: 7%, ginger paste: 200 g). Konsentrat given in the morning as much as 1% of the weight of the cattle based on dry matter, while the grass given a minimum of 10% of the weight of livestock observation variables include: fatty acid composition of meat. Data the analyzies qualitative. The results of the study showed that the composition of saturated fatty acids in meat decreased and an increase in unsaturated fatty acids, namely linoleic acid (omega 6) and linolenic acid (omega 3), and deikosapenta deikosaheksa acid.Keywords :

Meat lipid profile of steers finished in pearl millet pasture with different rates of concentrate

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2013000500012 ◽

2013 ◽

Vol 48 (5) ◽

pp. 553-558 ◽

Cited By ~ 2

Author(s):

Luis Fernando Glasenapp de Menezes ◽

Luciane Rumpel Segabinazzi ◽

João Restle ◽

Leandro da Silva Freitas ◽

Ivan Luiz Brondani ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Lipid Profile ◽

Pearl Millet ◽

Unsaturated Fatty Acids ◽

Vaccenic Acid ◽

Live Weight ◽

Beef Steers ◽

Omega 3 ◽

Omega 6

The objective of this work was to evaluate the meat lipid profile from Devon beef steers finished in pearl millet (Pennisetum americanum) pasture and fed at different rates of concentrate supplementary diet. Twelve steers weighing 270 kg, at 12‑month‑average initial age, were randomly distributed into three treatments: pearl millet pasture; and pearl millet pasture plus a concentrate equivalent at 0.5 or 1.0% of body weight, with two replicates. Total contents of saturated and unsaturated fatty acids, the polyunsaturated:saturated ratio and other relevant fatty acids as the vaccenic acid, conjugated linoleic acid, omega‑3, and omega‑6 were not affected by the consumption of a concentrate supplement at 0.5 or 1.0% live weight. However, the 0.5% supplementation level reduced the concentration of dihomo‑γ‑linolenic fatty acid (C20: 3 n‑6), while the 1.0% supplementation level elevated the content of docosahexaenoic (DHA) (C22: 6 n‑3) fatty acid, and the omega‑6:omega‑3 ratio in meat. Consumption of up to 1.0% energy supplementation increases the omega‑6:omega‑3 ratio in meat from Devon steers grazing on pearl millet pasture.

Inclusion of soybean and linseed oils in the diet of lactating dairy cows makes the milk fatty acid profile nutritionally healthier for the human diet

PLoS ONE ◽

10.1371/journal.pone.0246357 ◽

2021 ◽

Vol 16 (2) ◽

pp. e0246357

Author(s):

Mauricio X. S. Oliveira ◽

Andre S. V. Palma ◽

Barbara R. Reis ◽

Camila S. R. Franco ◽

Alessandra P. S. Marconi ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Soybean Oil ◽

Fatty Acid Profile ◽

Milk Fat ◽

Unsaturated Fatty Acids ◽

Linseed Oil ◽

Human Consumption ◽

Omega 3 ◽

Omega 6

Fluid milk and its derivatives are important dietary ingredients that contribute to daily nutrient intake of the modern Homo sapiens. To produce milk that is healthier for human consumption, the present study evaluated the effect of adding soybean oil and linseed oil in the diet of lactating cows. The fatty acid profile of milk, milk composition, and the blood parameters of cows were evaluated. Eighteen Holstein cows were distributed in a replicated Latin square design and distributed according to the following treatments: 1) Control (CC): traditional dairy cow diet, without addition of oil; 2) Soybean oil (SO): 2.5% addition of soybean oil to the traditional diet, as a source of omega-6; 3) Linseed oil (LO): 2.5% addition of linseed oil in the diet as a source of omega-3. Milk production was not affected, but oil supplementation decreased feed intake by 1.93 kg/cow/day. The milk fat percentage was significantly lower when cows were supplemented with vegetable oil (3.37, 2.75 and 2.89% for CC, SO and LO, respectively). However, both soybean and linseed oils decreased the concentration of saturated fatty acids (66.89, 56.52 and 56.60 g/100g for CC, SO and LO respectively), increased the amount of unsaturated fatty acids in milk (33.05, 43.39, and 43.35 g/100g for CC, SO and LO respectively) and decreased the ratio between saturated/unsaturated fatty acids (2.12, 1.34, and 1.36 for CC, SO and LO respectively). Furthermore, SO and LO increased significantly the concentration of monounsaturated fatty acids (29.58, 39.55 and 39.47 g/100g for CC, SO and LO respectively), though it did not significantly alter the level of polyunsaturated fatty acids in milk fat (3.57, 3.93 and 3.98 g/100g for CC, SO and LO respectively). Supplementation with LO enhanced the concentration of omega-3 fatty acids on milk (0.32, 0.36, and 1.02 for CC, SO and LO respectively). Blood variables aspartate aminotransferase, gamma glutamyl transferase, urea, albumin, creatinine and total proteins were not altered. On the other hand, total cholesterol, HDL and LDL were greater in the group supplemented with vegetable oils. Supplementation with vegetable oils reduced the dry matter intake of cows, the fat content of milk, and improved saturated/unsaturated fatty acid ratio of milk fat. Compared to the SO treatment, animals fed LO produced milk with greater content of omega-3, and a more desirable omega-6/omega-3 ratio on a human nutrition perspective. Thus, the inclusion of SO and LO in the diet of lactating dairy cows makes the milk fatty acid profile nutritionally healthier for the human consumption.

Domestic chicken omega 3 – a product for promoting human health

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/854/1/012081 ◽

2021 ◽

Vol 854 (1) ◽

pp. 012081

Author(s):

Dragan Sefer ◽

Stamen Radulovic ◽

Dejan Peric ◽

Matija Sefer ◽

Lazar Makivic ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Linolenic Acid ◽

Fatty Acid Profile ◽

Unsaturated Fatty Acids ◽

Daily Intake ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Wide Range ◽

Omega 6

Abstract Literature data show that the relationship between two groups of polyunsaturated fatty acids in diet, omega 3 acids, whose basic representative is a-linolenic acid (C18: 3 n-3), and omega 6 acids, whose basic representative is linoleic acid (C18: 2 n-6), has a significant role in development of cardiovascular diseases in humans. The optimal ratio of omega 6 to omega 3 fatty acids is around 4:1. In monogastric animals, the fatty acids in feed are absorbed in the gastrointestinal tract largely unchanged. This means the fatty acid profile of the animal’s diet directly reflects the fatty acid profile of the tissue. The daily intake of unsaturated fatty acids can be increased by an adequate animal nutrition strategy. Flaxseed contains ten times more unsaturated (32.26%) than saturated (3.66%) fatty acids. The largest amount of unsaturated fatty acids (about 70%) is a-linolenic acid (ALA), which is a precursor of the entire omega 3 series of fatty acids, and which makes flaxseed an ideal raw material for the production of a wide range of omega 3 enriched products. In order to obtain chicken meat rich in omega 3, an experiment was organized with a specific diet for broilers at fattening. Thanks to the designed animal feed, it was possible to get products (meat, breast, drumstick, liver, subcutaneous fat) with significantly higher amounts of omega 3 fatty acids compared to the same products obtained from broilers fed with conventional mixtures, or with almost the ideal ratio between omega 6 and omega 3 fatty acids.

The Concentration of Polyunsaturated Fatty Acid in Palm Oil by Urea Complexation

EKUILIBRIUM Journal of Chemical Engineering ◽

10.20961/equilibrium.v2i1.40430 ◽

2018 ◽

Vol 2 (1) ◽

Author(s):

Dwi Ardiana Setyawardhani ◽

Margono Margono ◽

Ardi Pratama ◽

Fermanditya Petratama

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Linoleic Acid ◽

Free Fatty Acid ◽

Palm Oil ◽

Unsaturated Fatty Acid ◽

Urea Complexation ◽

Omega Fatty Acid ◽

Omega 6 ◽

Poly Unsaturated Fatty Acid

<p>Linoleic acid is a Poly-Unsaturated Fatty Acid (PUFA) that corresponds to Omega 6 Fatty Acid. This is the major component of vegetable oil and very important for health. As an essential fatty acid, linoleic acid has to be obtained from foods. Consumption of omega fatty acid in the form of concentrate has more benefit than the whole oil. Fulfilling this requirement, effective method to separate PUFAs from vegetable oils is needed. One of the favorable methods is urea complexation. Palm oil was used as the fatty acids resource due to its potential feedstock in Indonesia. This aim of this research is to study the effect of urea, ethanol and free fatty acid (FFA) ratios during complexation of palm oil. This research was developed in three steps, 1) free fatty acid preparation from palm oil (saponification process), 2) crystallization of the fatty acids mixture and 3) filtration for separating the unsaturated fatty acid. Concentrated linoleic acid is obtained in the liquid phase, while the saturated fatty acid is obtained in the solid. This research resulted that the concentration of PUFA was increasing as the ratio of urea, FFA and ethanol increases.</p>

Komposisi Asam Lemak, Angka Peroksida, dan Angka TBA Fillet Ikan Kakap (Lutjanus sp) pada Suhu dan Lama Penyimpanan Berbeda

Jurnal Agritech ◽

10.22146/agritech.11212 ◽

2018 ◽

Vol 37 (3) ◽

pp. 319 ◽

Cited By ~ 1

Author(s):

Rahim Husain ◽

Suparmo Suparmo ◽

Eni Harmayani ◽

Chusnul Hidayat

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Unsaturated Fatty Acid ◽

Unsaturated Fatty Acids ◽

Storage Temperature ◽

Saturated Fatty Acids ◽

Fish Lipid ◽

C 30 ◽

Poly Unsaturated Fatty Acid ◽

Total Pufa

Fish has a high nutritional value and is a major food source in many countries. Fish lipid has a high content of polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (EPA; 20: 5n-3) and docosahexsanoic acid (DHA; 22: 6n-3). The objective of this research was to determine fatty acids composition of snapper (Lutjanus sp) fillet and its damage during the storage process. The results showed that total of the saturated fatty acids (SFA) increased from 4.35% to 25.55%, 28.06%, 32.73%, and 61.75% during storage at 0 °C, 10 °C, 20 °C, 30 °C, and 40 °C, respectively. Total mono-unsaturated fatty acids (MUFA) were 23.72%, 23.69%, 14.4%, 22.66%, and 29.4% at storage temperature of 0 °C, 10 °C, 20 °C, 30 °C, and 40 °C. Total PUFA decreased from 25.06% to 15.98%, 14.99%, 10.32%, and 8.84% at 0 °C, 10 °C, 20 °C, 30 °C, and 40 °C. Peroxide value, as primary peroxide of snapper fillet, increased about 10.60 times with an increased in storage temperature from 0 °C to 40 °C. Value of TBA increased 6.60 times with an increased in temperature from 0 °C to 40 °C during 45 days.ABSTRAKIkan memiliki nilai gizi tinggi dan merupakan sumber makanan utama di banyak negara. Lipid ikan memiliki kandungan tinggi asam tak jenuh ganda (Poly Unsaturated Fatty Acid, PUFA), terutama asam eikosapentanoat (EPA; 20:5n-3) dan asam docosahexsanoat (DHA; 22:6n-3). Penelitian ini bertujuan untuk mengetahui komposisi asam lemak fillet ikan kakap (Lutjanus sp) dan kerusakan akibat proses penyimpanan. Hasil analisis asam lemak jenuh (Saturated Fatty Acid, SFA) menunjukkan bahwa asam lemak jenuh meningkat dari 4,35% menjadi 25,55%, 28,06%, 32,73%, dan 61,75% selama penyimpanan pada 0 °C, 10 °C, 20 °C, 30 °C, dan 40 °C. Total asam lemak tak jenuh (Mono Unsaturated Fatty Acid, MUFA) adalah 23,72%, 23,69, 14,4%, 22,66%, dan 29,4% pada penyimpanan 0 °C, 10 °C, 20 °C, 30 °C, dan 40 °C. Sedangkan total PUFA turun dari 25,05% menjadi 15,98%, 14,99%, 10,32%, dan 8,84% pada penyimpanan 0 °C, 10 °C, 20 °C, 30 °C, dan 40 °C. Angka peroksida sebagai produk primer dari oksidasi fillet ikan kakap meningkat 10,6 kali dengan kenaikan suhu dari 0 °C sampai 40 °C. Angka TBA meningkat 6,6 kali dari suhu 0 °C ke suhu 40 °C pada lama penyimpanan 45 hari.Kata kunci: Komposisi asam lemak; fillet ikan kakap (Lutjanus sp); angka peroksida; angka TBA

Content of Higher Fatty Acids in Green Vegetables

Czech Journal of Food Sciences ◽

10.17221/621-cjfs ◽

2009 ◽

Vol 27 (Special Issue 1) ◽

pp. S125-S129 ◽

Cited By ~ 17

Author(s):

R. Vidrih ◽

S. Filip ◽

J. Hribar

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Acid Content ◽

Unsaturated Fatty Acids ◽

Fatty Acid Content ◽

Omega 3 ◽

Omega 6 ◽

Radish Sprouts ◽

Total Fatty Acids ◽

Higher Fatty Acids

Green vegetables are considered an important source of some nutritionally important constituents that have health benefits (e.g. vitamins, minerals, antioxidants, fibre). Epidemiological data suggest that consuming a diet rich in fruit and vegetables can lower the risks for chronic diseases, such as cardiovascular diseases and cancer. Over the past 100–150 years, there have been enormous increases in the consumption of omega-6 fatty acids due to the increased intake of vegetable oils from various seeds. Studies have indicated that a high intake of omega-6 fatty acids shifts the physiological state to one that is prothrombotic and pro-aggregatory, whereas omega-3 fatty acids have anti-inflammatory, antithrombotic, anti-arrhythmic, hypolipidemic and vasodilatory properties. Literature data regarding the contents of higher fatty acids (e.g. omega-6 fatty acids) in vegetables are scarce, although vegetables are known to contain a high proportion of n-3 fatty acids. Here, the fatty acid content and composition was determined for 26 green vegetables that are commonly available in Slovenia, by gas-liquid chromatography and <I>in situ</I> transesterification. The fatty acid analysis revealed C16:0, C16:1, C18:0, C18:1, C18:2n-6 and C18:3n-3. The total fatty acid content in the vegetables ranged from 500 mg/100 g fresh weight (f.w.) in red cabbage, to 4.000 mg/100 g f.w. in tarragon. The proportion of saturated fatty acids (as g/100 g total fatty acids) ranged from 12% to 35%. All of the vegetables contained a high proportion of poly-unsaturated fatty acids (PUFAs), ranging from 45% to 81% of total fatty acids. The omega-3 PUFA proportion ranged from 5% in carrot to 60% in tarragon. The content of mono-unsaturated fatty acids ranged from 1% to 25%. French beans, tarragon and radish sprouts contained the highest concentrations of C16:1, at 5 mg/100 g f.w. Consumption of 100 g of tarragon meets 13.2% of daily requirements for α-linolenic acid; similarly, for radish sprouts 9.4%, for mangold 6.9%, for ruccola 5.4%, for green salad 5.0%, and for kale 4.7%. Green vegetables are an important source of 18:3n-3 PUFAs, especially for vegetarian populations.

The highly unsaturated fatty acids: physiological role and clinical possibilities

Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie ◽

10.4102/satnt.v25i4.169 ◽

2006 ◽

Vol 25 (4) ◽

pp. 266-283

Author(s):

M. Haag ◽

M. Coetzee

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Cell Membrane ◽

Unsaturated Fatty Acids ◽

Physiological Role ◽

Dietary Lipids ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Positive Effects ◽

Omega 6

Fatty acids play a very important role in human physiology. Except for the fact that fatty acids in stored triglycerides can be used for energy production, fatty acids from dietary lipids are transported in plasma and built into various cellular structures. The fatty acid profile of cell membrane phospholipids plays a determining role in the cell membrane in that it influences fluidity, receptor function and the type of eicosanoids that can be synthesised from it. Fatty acids also have highly differentiated inputs in cellular transduction mechanisms and regulation of gene transcription. In this, the steric conformation plays a large role: the straight-chain saturated and trans-fatty acids, when compared with the unsaturated fatty acids with progressively more bent chains, lead to a variety of mechanisms that have less positive effects on our health. In this regard, the two most important families of polyunsaturated fatty acids, the so-called omega-3 and omega-6 fatty acids, also lead to different effects. Changes in the pattern of dietary fatty acid intake through the ages have lead to an increased intake of saturated, trans- and omega-6 fatty acids relative to omega-3 fatty acids. Clinical trials during the past twenty years have shown that dietary supplementation with omega-3 fatty acids can lead to an improvement of the symptoms of certain lifestyleassociated disorders. Atherosclerosis, obesity, type 2 diabetes mellitus, depression, schizophrenia, Alzheimer’s disease, attention deficit hyperactivity disorder in young children, certain types of cancer, osteoporosis in the elderly and some dermatological disorders are amongst the conditions that can be addressed in this manner.

IDENTIFICATION AND CHARACTERIZATION OF A PURPLE NONSULFUR BACTERIUM ISOLATED FROM COASTAL AREA OF HAI PHONG FOR USING IN PRODUCTION OF UNSATURATED FATTY ACID (OMEGA 6, 7, 9)

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/57/6/13711 ◽

2019 ◽

Vol 57 (6) ◽

pp. 665 ◽

Cited By ~ 1

Author(s):

Yen Thi Hoang ◽

Quynh Thi Thu Tran ◽

Ha Hoang Chu ◽

Tuyen Thi Do ◽

Thanh Tat Dang ◽

...

Keyword(s):

Fatty Acid ◽

Unsaturated Fatty Acid ◽

Unsaturated Fatty Acids ◽

Nitrogen Sources ◽

16S Rrna Analysis ◽

Rhodovulum Sulfidophilum ◽

Purple Nonsulfur Bacterium ◽

High Lipid ◽

Physiological Properties ◽

Omega 6

Purple nonsulfur bacteria are a group that has so much biotechnological applications, particularly in producing of functional food rich with unsaturated fatty acids. A purple nonsulfur bacterium (named HPB.6) was chosen based on its strong growth, high lipid and synthesis of unsaturated fatty acid (omega 6,7,9). Studying on basic biological characteristics showed that the cells of HPB.6 were observed as ovoid-rod shape, none motility, Gram negative staining. The diameter of single bacterium was about 0.8-1.0 µm. The cells divide by binary fission and had bacteriochlorophyll a (Bchl a). This bacterium grew well on medium with carbon and nitrogen sources such as acetate, succinate, pyruvate, butyrate, glutamate, arginine, leucine, tyrosine, alanine, methionine, threonine, glutamine, yeast extract and NH4Cl. This selected strain grew well on medium with salt concentrations from 1.5 - 6.0% (optimum 3%), pH from 5.0 to 8.0 (optimum at pH 6.5) and could withstand Na2S at 4.0 - 5.2 mM. Based on morphological, physiological properties and 16S rRNA analysis received demonstrated that HPB.6 strain belongs to the species Rhodovulum sulfidophilum.