scholarly journals Hot water extract of turmeric (Curcuma longa) prevents non-alcoholic steatohepatitis in mice by inhibiting hepatic oxidative stress and inflammation

2018 ◽  
Vol 7 ◽  
Author(s):  
Ryusei Uchio ◽  
Shinji Murosaki ◽  
Hiroshi Ichikawa
Keyword(s):  
Oxidative Stress ◽  
Curcuma Longa ◽  
Water Extract ◽  
Hot Water ◽  
Control Group ◽  
Type I ◽  
Hepatic Expression ◽  
Alcoholic Steatohepatitis ◽  
Hepatic Oxidative Stress ◽  
Hot Water Extract

AbstractCurcuma longa, also known as turmeric, has long been used as a medicinal herb with various biological effects. A hot water extract of C. longa (WEC) has been reported to show antioxidant and anti-inflammatory activity, but its effect on hepatic inflammation is poorly understood. In the present study, to investigate the effect of WEC on non-alcoholic steatohepatitis, C57BL/6J mice were fed a low-methionine, choline-deficient diet with 0·175 % WEC (WEC group) or without WEC (control group) for 6 or 12 weeks. Although hepatic steatosis was similar in the WEC group and the control group, WEC suppressed the elevation of plasma aspartate aminotransferase and alanine aminotransferase, which are markers of hepatocellular damage. Compared with the control group, the WEC group had higher hepatic levels of reduced glutathione and superoxide dismutase, as well as a lower hepatic level of thiobarbituric acid-reactive substances. WEC also reduced hepatic expression of mRNA for inflammatory factors, including TNF-α, IL-1β, IL-6, monocyte chemoattractant protein-1, vascular cell adhesion molecule-1, F4/80 and CC motif chemokine receptor 2. Histological examination revealed that WEC suppressed hepatic recruitment of F4/80+ monocytes/macrophages and inhibited hepatic fibrosis. Furthermore, WEC inhibited hepatic expression of mRNA for molecules related to fibrosis, such as transforming growth factor-β, α-smooth muscle actin, type I collagen (α1-chain) and tissue inhibitor of matrix metalloproteinase-1. These findings suggest that dietary intake of WEC prevents the progression of non-alcoholic steatohepatitis by alleviating hepatic oxidative stress and inflammation.

scholarly journals A hot water extract of turmeric (Curcuma longa) suppresses acute ethanol-induced liver injury in mice by inhibiting hepatic oxidative stress and inflammatory cytokine production

2017 ◽  
Vol 6 ◽  
Author(s):  
Ryusei Uchio ◽  
Yohei Higashi ◽  
Yusuke Kohama ◽  
Kengo Kawasaki ◽  
Takashi Hirao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Oral Administration ◽  
Alanine Aminotransferase ◽  
Curcuma Longa ◽  
Water Extract ◽  
Hot Water ◽  
Hepatic Tissue ◽  
Hot Water Extract ◽  
Acute Ethanol

AbstractTurmeric (Curcuma longa) is a widely used spice that has various biological effects, and aqueous extracts of turmeric exhibit potent antioxidant activity and anti-inflammatory activity. Bisacurone, a component of turmeric extract, is known to have similar effects. Oxidative stress and inflammatory cytokines play an important role in ethanol-induced liver injury. This study was performed to evaluate the influence of a hot water extract of C. longa (WEC) or bisacurone on acute ethanol-induced liver injury. C57BL/6 mice were orally administered WEC (20 mg/kg body weight; BW) or bisacurone (60 µg/kg BW) at 30 min before a single dose of ethanol was given by oral administration (3·0 g/kg BW). Plasma levels of aspartate aminotransferase and alanine aminotransferase were markedly increased in ethanol-treated mice, while the increase of these enzymes was significantly suppressed by prior administration of WEC. The increase of alanine aminotransferase was also significantly suppressed by pretreatment with bisacurone. Compared with control mice, animals given WEC had higher hepatic tissue levels of superoxide dismutase and glutathione, as well as lower hepatic tissue levels of thiobarbituric acid-reactive substances, TNF-α protein and IL-6 mRNA. These results suggest that oral administration of WEC may have a protective effect against ethanol-induced liver injury by suppressing hepatic oxidation and inflammation, at least partly through the effects of bisacurone.

scholarly journals Effect of Curcuma longa Hot Water Extract on Activity of Neuronal Cells Related to Oxidative Stress

2012 ◽  
Vol 22 (5) ◽  
pp. 657-664
Author(s):  
Yong-Byung Chae ◽  
Kyung-Tae Chung ◽  
Sung-Goo Kim ◽  
Byung-Hong Yoo ◽  
Moon-Moo Kim
Keyword(s):  
Oxidative Stress ◽  
Curcuma Longa ◽  
Neuronal Cells ◽  
Water Extract ◽  
Hot Water ◽  
Hot Water Extract

scholarly journals A Hot Water Extract of Sideritis scardica Prolongs Life Span and Enhances Heat Shock Resistance in Caenorhabditis elegans

2020 ◽  
Vol 15 (4) ◽  
pp. 1934578X2091728
Author(s):  
Yoshihiko Nishioka ◽  
Seiya Nishikawa ◽  
Toshiyuki Shibata
Keyword(s):  
Heat Stress ◽  
Caenorhabditis Elegans ◽  
Stress Response ◽  
Heat Shock ◽  
Life Span ◽  
Water Extract ◽  
Hot Water ◽  
Control Group ◽  
C Elegans ◽  
Hot Water Extract

Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. The tea of S. scardica has been handed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. In this study, we prepared a hot water extract of S. scardica (SHWE) and examined its effects on both life span and stress response in living tissue using Caenorhabditis elegans and its transgenic mutants. The life span of wild-type N2 worms was prolonged by approximately 15% at the SHWE concentration of 5 µg/mL and approximately 22% at the SHWE concentration of 50 µg/mL, as compared with the control group. The effect of SHWE on the expression of heat shock protein 16.2 (HSP-16.2) under heat stress was investigated using TJ375 worms, a transgenic mutant of C. elegans. In the TJ375 worms pretreated with SHWE, the fluorescence intensity of green fluorescent protein fluorescence, which indicates the expression of HSP-16.2, was significantly increased. In the assay using TJ356 worms, the worms pretreated with SHWE did not show the translocation of DAF-16, a forkhead transcription factor class O homolog, from the cytoplasm to nucleus under heat stress. Additionally, under heat stress, the pretreatment of SHWE improved the survival rate of GR1307 worms, a knockout mutant of daf-16. These results indicate that SHWE enhances HSP-16.2 expression through a stress-response pathway (eg, HSF-1 pathway) other than the DAF-16 pathway, resulting in a prolonged life span of C. elegans under heat stress.

scholarly journals Protective Effect of Ferments of Hot-water Extract Mixture from Rhodiola sachalinensis and Red Ginseng on Oxidative Stress-induced C2C12 Myoblast

2013 ◽  
Vol 26 (3) ◽  
pp. 485-491
Author(s):  
Bo-Ra Yoon ◽  
Young-Hyun Kim ◽  
Jong-Seok Lee ◽  
Hee-Do Hong ◽  
Young-Kyoung Rhee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Water Extract ◽  
Hot Water ◽  
C2c12 Myoblast ◽  
Red Ginseng ◽  
Rhodiola Sachalinensis ◽  
Hot Water Extract

Juzentaihoto hot water extract alleviates muscle atrophy and improves motor function in streptozotocin-induced diabetic oxidative stress mice

2018 ◽  
Vol 73 (1) ◽  
pp. 202-209 ◽  
Author(s):  
Tomoaki Ishida ◽  
Michiro Iizuka ◽  
Yanglan Ou ◽  
Shumpei Morisawa ◽  
Ayumu Hirata ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Motor Function ◽  
Muscle Atrophy ◽  
Water Extract ◽  
Hot Water ◽  
Hot Water Extract

scholarly journals Effects of Extracts from Tiaozhi Granule and Its Components on Expression of Scavenger Receptor Class B Type I

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Xiao Yu ◽  
Xiao-Dong Zhao ◽  
Rong-Qi Bao ◽  
Jia-Yu Yu ◽  
Guo-Xing Zhang ◽  
...  
Keyword(s):  
Promoter Activity ◽  
Curcuma Longa ◽  
Water Extract ◽  
Luciferase Reporter ◽  
Control Group ◽  
Type I ◽  
Dependent Manner ◽  
Alcohol Extract ◽  
Water Extracts ◽  
Gene Assay

Sera from the rats with different drug treatments (atorvastatin, Tiaozhi granule, or its extracts) were collected. LO-2 cells or HepG2 cells were pretreated with different sera as the following groups randomly: (1) blank control group, (2) positive control group (atorvastatin group), (3) Tiaozhi granule water extract groups, (4) Tiaozhi granule alcohol extract groups, and (5) alcohol extracts for each component:Pollen Typhae Angustifoliae,Curcuma longaL., andRhizoma Alismatis. LO-2 cells were cotransfected with plasmid carrying SR-BI and pRL-TK promoter genes. Promoter activity was measured by the luciferase reporter gene assay. The mRNA and protein expressions of SR-BI were examined using real-time PCR and western blot analyses. Our results show that promoter activity and mRNA and protein expression levels of the SR-BI were significantly upregulated by Tiaozhi granules alcohol or water extracts in a dose-dependent manner.Pollen Typhae Angustifoliaealcohol extract with a high dosage could also increase SR-BI activity and expression, but not the extracts fromCurcuma longaL. andRhizoma Alismatis. Both Tiaozhi granule alcohol and water extracts can upregulate SR-BI gene expression. Among the components,Pollen Typhae Angustifoliaeare important for the regulatory effect coordinating withCurcuma longaL. andRhizoma Alismatis.

scholarly journals GAMBARAN HISTOPATOLOGIK HATI TIKUS WISTAR YANG DIBERIKAN AIR REBUSAN DAUN SENDOK (PLANTAGO MAJOR) PASCA INDUKSI KARBON TETRAKLORIDA (CCl4)

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Pingkan Lintong
Keyword(s):  
Wistar Rats ◽  
Water Extract ◽  
Positive Control ◽  
Liver Cells ◽  
Negative Control ◽  
Hot Water ◽  
Control Group ◽  
Plantago Major ◽  
Hot Water Extract ◽  
Microscopic Features

Abstract: The common plantain (plantago major) contains the compound aucubin which has been demonstrated to have theraupetic effects in improving liver cells as hepatoprotector. The objective of this study was to reveal the histopathological features of the liver of wistar rats administered with the hot water extract of Plantago major after induction with carbon tetrachloride (CCl4). This study was a laboratory experimental research employing 14 wistar rats; two rats were used as the negative control fed with regular pellets for 5 days, two rats as the positif control fed with pellets containing CCl4 of 0.05 cc for five days, five rats as treatment group receiving pellets containing CCl4 of 0.05 cc for five days and were fed with regular pellets afterwards, and five rats received pellets containing CCl4 of 0.05 cc for five days and were administered with the hot water extract of Plantago major 0f 1.6 cc. Results showed that rats in the negative control exhibited normal macroscopic and microscopic features. In the positive control group, the microscopic features demonstrated liver cells with steatosis.  Meanwhile, the rats administered with the water extract of Plantago major showed liver cells which had regenerated. It is concluded that the administration of the water extract of Plantago major can improve the liver cells by diffuse regeneration. Keywords: plantago major, regeneration, steatosis , wistar rats     Abstrak: Daun sendok (Plantago major) mengandung senyawa aucubin yang telah terbukti berkhasiat memperbaiki fungsi sel hati dan sebagai hepatoprotektor. Tujuan penelitian ini adalah untuk melihat gambaran histopatologik hati tikus wistar yang diberikan air rebusan daun sendok pasca induksi karbon tetraklorida (CCl4). Penelitian ini merupakan penelitian eksperimental laboratorium dengan menggunakan 14 ekor tikus wistar, dua tikus digunakan sebagai kontrol negatif dengan pemberian pellet biasa selama 5 hari, dua tikus sebagai kontrol positif yang diberikan pellet yang mengandung CCl4 dosis 0,05 cc selama 5 hari, lima tikus sebagai kelompok perlakuan dengan pemberian CCl4 dosis 0,05 cc selama 5 hari dilanjutkan pellet biasa, dan lima tikus diberikan CCl4 0,05 cc selama 5 hari dilanjutkan pemberian air rebusan daun sendok dosis 1,6 cc. Hasil penelitian ini menunjukkan gambaran makroskopik dan mikroskopik normal pada kelompok kontrol negatif. Pada kelompok kontrol positif gambaran mikroskopiknya menunjukkan sel hati dengan steatosis. Kelompok tikus yang diberikan air rebusan daun sendok gambaran mikroskopiknya menunjukkan sel-sel hati yang mengalami regenerasi. Disimpulkan bahwa pemberian air rebusan daun sendok memperlihatkan regenerasi hati secara difus. Kata kunci: daun sendok, regenerasi, steatosis, tikus wistar.

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