scholarly journals GAMBARAN HISTOPATOLOGIK HATI TIKUS WISTAR YANG DIBERIKAN AIR REBUSAN DAUN SENDOK (PLANTAGO MAJOR) PASCA INDUKSI KARBON TETRAKLORIDA (CCl4)

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Pingkan Lintong
Keyword(s):  
Wistar Rats ◽  
Water Extract ◽  
Positive Control ◽  
Liver Cells ◽  
Negative Control ◽  
Hot Water ◽  
Control Group ◽  
Plantago Major ◽  
Hot Water Extract ◽  
Microscopic Features

Abstract: The common plantain (plantago major) contains the compound aucubin which has been demonstrated to have theraupetic effects in improving liver cells as hepatoprotector. The objective of this study was to reveal the histopathological features of the liver of wistar rats administered with the hot water extract of Plantago major after induction with carbon tetrachloride (CCl4). This study was a laboratory experimental research employing 14 wistar rats; two rats were used as the negative control fed with regular pellets for 5 days, two rats as the positif control fed with pellets containing CCl4 of 0.05 cc for five days, five rats as treatment group receiving pellets containing CCl4 of 0.05 cc for five days and were fed with regular pellets afterwards, and five rats received pellets containing CCl4 of 0.05 cc for five days and were administered with the hot water extract of Plantago major 0f 1.6 cc. Results showed that rats in the negative control exhibited normal macroscopic and microscopic features. In the positive control group, the microscopic features demonstrated liver cells with steatosis.  Meanwhile, the rats administered with the water extract of Plantago major showed liver cells which had regenerated. It is concluded that the administration of the water extract of Plantago major can improve the liver cells by diffuse regeneration. Keywords: plantago major, regeneration, steatosis , wistar rats     Abstrak: Daun sendok (Plantago major) mengandung senyawa aucubin yang telah terbukti berkhasiat memperbaiki fungsi sel hati dan sebagai hepatoprotektor. Tujuan penelitian ini adalah untuk melihat gambaran histopatologik hati tikus wistar yang diberikan air rebusan daun sendok pasca induksi karbon tetraklorida (CCl4). Penelitian ini merupakan penelitian eksperimental laboratorium dengan menggunakan 14 ekor tikus wistar, dua tikus digunakan sebagai kontrol negatif dengan pemberian pellet biasa selama 5 hari, dua tikus sebagai kontrol positif yang diberikan pellet yang mengandung CCl4 dosis 0,05 cc selama 5 hari, lima tikus sebagai kelompok perlakuan dengan pemberian CCl4 dosis 0,05 cc selama 5 hari dilanjutkan pellet biasa, dan lima tikus diberikan CCl4 0,05 cc selama 5 hari dilanjutkan pemberian air rebusan daun sendok dosis 1,6 cc. Hasil penelitian ini menunjukkan gambaran makroskopik dan mikroskopik normal pada kelompok kontrol negatif. Pada kelompok kontrol positif gambaran mikroskopiknya menunjukkan sel hati dengan steatosis. Kelompok tikus yang diberikan air rebusan daun sendok gambaran mikroskopiknya menunjukkan sel-sel hati yang mengalami regenerasi. Disimpulkan bahwa pemberian air rebusan daun sendok memperlihatkan regenerasi hati secara difus. Kata kunci: daun sendok, regenerasi, steatosis, tikus wistar.

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Acute and Subchronic Toxicity Profile of a Polyherbal Drug Used in Sri Lankan Traditional Medicine

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Donisha Shani Niharika Keembiya Liyanagamage ◽  
Susanthi Jayasinghe ◽  
Anoja Priyadarshani Attanayake ◽  
Veranja Karunaratne
Keyword(s):  
Acute Toxicity ◽  
Glucose Concentration ◽  
Wistar Rats ◽  
Water Extract ◽  
Diabetic Rats ◽  
Acetone Extract ◽  
Hot Water ◽  
Piper Nigrum ◽  
Hot Water Extract ◽  
Acetone Extracts

A polyherbal drug composed of leaves of Murraya koenigii L. Spreng, cloves of Allium sativum L., fruits of Garcinia quaesita Pierre, and seeds of Piper nigrum L. is a popular drug which has been used by indigenous practitioners in Sri Lanka for the treatment of diabetes mellitus and dyslipidemia. The acute toxicity assessment was conducted, following a single oral dose of 0.25–2.0 g/kg in healthy rats, and rats were observed up to 14 days. The hot water extract (1.0 g/kg) and the water : acetone extract (0.5, 1.0, and 1.5 g/kg) were administered to Wistar rats for 28 days in the subchronic study. Hypoglycemic and antihyperglycemic activities (dose response studies) of cold water, hot water, and water : acetone extracts of the polyherbal mixture were evaluated at the doses of 0.5, 1.0, and 1.5 g/kg in healthy and streptozotocin-induced diabetic rats (70 mg/kg, ip), respectively. Acute toxicity study showed that the polyherbal drug did not cause any change in animals throughout the experimental period of 14 days. The administration of the hot water extract and the water : acetone extract of the polyherbal drug for 28 days did not produce changes in the selected biochemical and hematological parameters in Wistar rats (p > 0.05). The histological assessment corroborated the biochemical findings with no significant treatment-related changes in the kidney and liver. The treatment of polyherbal drug significantly lowered the serum glucose concentration compared to the diabetic control rats (p < 0.05) while it did not lead to a severe reduction of glucose concentration in healthy rats. The hot water and water : acetone extracts of the polyherbal drug showed a statistically significant improvement on total area under the glucose tolerance curve in diabetic rats (p < 0.05), reflecting dose-dependent antihyperglycemic effects of the drug. Based on the results, we conclude that the aforementioned antidiabetic polyherbal remedy is free of toxic/adverse effects at the equivalent human therapeutic dose in healthy Wistar rats and would be a safe therapeutic agent for long-term treatments.

scholarly journals A Hot Water Extract of Sideritis scardica Prolongs Life Span and Enhances Heat Shock Resistance in Caenorhabditis elegans

2020 ◽  
Vol 15 (4) ◽  
pp. 1934578X2091728
Author(s):  
Yoshihiko Nishioka ◽  
Seiya Nishikawa ◽  
Toshiyuki Shibata
Keyword(s):  
Heat Stress ◽  
Caenorhabditis Elegans ◽  
Stress Response ◽  
Heat Shock ◽  
Life Span ◽  
Water Extract ◽  
Hot Water ◽  
Control Group ◽  
C Elegans ◽  
Hot Water Extract

Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. The tea of S. scardica has been handed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. In this study, we prepared a hot water extract of S. scardica (SHWE) and examined its effects on both life span and stress response in living tissue using Caenorhabditis elegans and its transgenic mutants. The life span of wild-type N2 worms was prolonged by approximately 15% at the SHWE concentration of 5 µg/mL and approximately 22% at the SHWE concentration of 50 µg/mL, as compared with the control group. The effect of SHWE on the expression of heat shock protein 16.2 (HSP-16.2) under heat stress was investigated using TJ375 worms, a transgenic mutant of C. elegans. In the TJ375 worms pretreated with SHWE, the fluorescence intensity of green fluorescent protein fluorescence, which indicates the expression of HSP-16.2, was significantly increased. In the assay using TJ356 worms, the worms pretreated with SHWE did not show the translocation of DAF-16, a forkhead transcription factor class O homolog, from the cytoplasm to nucleus under heat stress. Additionally, under heat stress, the pretreatment of SHWE improved the survival rate of GR1307 worms, a knockout mutant of daf-16. These results indicate that SHWE enhances HSP-16.2 expression through a stress-response pathway (eg, HSF-1 pathway) other than the DAF-16 pathway, resulting in a prolonged life span of C. elegans under heat stress.

scholarly journals PENGARUH EKSTRAK DAUN GEDI (Abelmoschus manihot L.) TERHADAP GAMBARAN HISTOPATOLOGI AORTA TIKUS WISTAR DENGAN DIET ATEROGENIK

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Nadya Nadiratika Papodi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Foam Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Blood Cholesterol ◽  
Control Group ◽  
Pork Lard ◽  
Pork Fat

Abstract: High blood cholesterol is a major risk factor for atherosclerosis. Gedi plants contain polyphenolic compounds that can lowering blood cholesterol levels. This study aimed to find out the effect of gedi (Abelmoschus manihot L) leaves extract on histopatologic features of aorta of those wistar rats, which had been administered an atherogenic diet. This was an experimental study on animal using 20 Wistar rats as samples. The rats were divided into 4 different groups, 5 rats for each group. Negative control group was fed only pelet, positive control group was fed pork fat as much as 2 ml/day, group C was fed on pork fat and gedi leaf extract, and group D was fed on pork fat then continued with gedi leaf extract. The results indicated that administration of 30 mg gedi leaf extract showed a histopathologic feature which is decreasing of foam cells. Conclusion: Microscopically, aorta of those rats fed on pork lard diet showed a number of foam cells in tunica intima and media whereas the rats that were fed on pork lard along with gedi leaf extract, generally there were no foam cells, but there aresome parts which contain few foam cells. On the group of Wistar rats which were fed on pork lard, continued with gedi leaf extract showed less foam cells compared to the positive control group.Keywords: Gedi leaf extract, pork lard, foam cell.Abstrak: Kadar kolesterol darah yang tinggi merupakan faktor risiko utama terjadinya aterosklerosis. Tumbuhan gedi mengandung senyawa polifenol yang dapat menurunkan kadar kolesterol darah. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun gedi terhadap gambaran histopatologi aorta tikus wistar dengan diet aterogenik. Hewan uji yang digunakan berupa 20 ekor tikus Wistar yang dibagi ke dalam 4 kelompok, masing-masing kelompok terdiri dari 5 ekor tikus. Kelompok kontrol negatif hanya diberikan pelet biasa, kelompok kontrol positif diberikan diet lemak babi 2ml/hari, kelompok C diberikan diet lemak babi bersamaan dengan ekstrak daun gedi, dan kelompok D diberikan lemak babi kemudian dilanjutkan dengan pemberian ekstrak daun gedi. Hasil penelitian memperlihatkan bahwa pemberian ekstrak daun gedi 30 mg mampu memberikan gambaran histopatologi aorta berupa berkurangnya jumlah sel busa. Simpulan: Secara mikroskopik, aorta tikus wistar yang diberi lemak babi menunjukkan adanya penumpukan sel-sel busa pada tunika intima dan media, sedangkan pada aorta tikus wistar yang diberi lemak babi bersamaan dengan ekstrak daun gedi maupun yang dilanjutkan dengan ekstrak daun gedi ditemukan berkurangnya jumlah sel-sel busa.Kata Kunci : Ekstrak daun gedi, Lemak babi, Sel busa.

scholarly journals Immunomodulatory and Anti-Inflammatory Potentials of Trigona Honey in the Therapy and Prevention against Respiratory Infection in Wistar Rats

2020 ◽  
Vol 11 (3) ◽  
pp. 2955-2962
Author(s):  
Ibrahim Khaled Al-kafaween ◽  
Abu Bakar Mohd Hilmi ◽  
Mohamed M. Soliman
Keyword(s):  
Respiratory Infection ◽  
Wistar Rats ◽  
Serum Levels ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Gene Expressions ◽  
Anti Inflammatory

Trigona honey (TH) is well known for its therapeutic characteristics. To date, the study of Trigona honey as a prophylactic or immune booster prior to the bacterial infection of the invivo model is not well covered. This study aims to investigate anti-inflammatory and immune activities in Wistar rats infected with respiratory infection following with Trigona honey. 25 Wistar rats were assigned to possitive groups, negative control group, positive control group was fed TH (5 g / kg body weight) orally, the untreated group was infected with Staphylococcus aureus to induce respiratory infection, the treated group has been infected with S. aureus followed by treatment with TH at a dose of 1.5 ×108 CFU / mL and the preventive group ingested TH one week before S. aureus infection. Blood was obtained for biochemical analysis. Lung tissues have been collected for molecular examination. The results showed a significant decrease in serum levels of ALT, AST, urea and creatinine in the preventive and treated groups, serum IgG increased significantly (P<0.05) in the preventive and treated groups, IFN-y increased in the preventive group while decreased in the treated group, and IL-8 increased in the treated group while decreased in the preventive group. The mRNA expression of AGP is up-regulated in the positive control, preventive and treated groups. The α2-MG, TNF-α , and mRNA expressions showed lower regulation after administration of TH in preventive and treated groups. The results show the ability of TH to counteract immune and inflammatory changes in serum levels and gene expressions.

scholarly journals Hypoglycemic activity test on smooth pigweed (Ammaranthus Hybridus L) leaf water extract on male Wistar rats

2021 ◽  
pp. 56-60
Author(s):  
Asti Yunia Rindarwati
Keyword(s):  
Wistar Rats ◽  
Water Extract ◽  
Positive Control ◽  
Hypoglycemic Activity ◽  
Control Group ◽  
White Rats ◽  
Male Wistar Rats ◽  
Tolerance Method ◽  
Smooth Pigweed

Introduction: Type 2 diabetes mellitus consists of an array of dysfunction characterized by hyperglycemia. The activity of smooth pigweed (Amaranthus hybridus L.) leaves water extract on male Wistar rats. Objectives: This research was started by supplying simplicia, making smooth pigweed leaves water extract, and testing the hypoglycemic activity of smooth pigweed leaves water extract on male Wistar rats. Methods: The glucose tolerance method was used to determine the hypoglycemic activity of smooth pigweed leaves water extract. Male white rats were divided into five groups of six rats each: a positive control group (0.5% of tragacanth suspension), a comparison group (Diabinese suspension at a dose of 22.5 mg/kg body weight (bw)), and three test groups at doses of 50 mg/kg bw, 100 mg/kg bw, and 150 mg/kg bw. Results and conclusions: The most significant hypoglycemic activity was seen with the dose of 150 mg/kg bw in comparison with the control group at 90 minutes.

scholarly journals UJI EFEK ANTIPIRETIK EKSTRAK MENIRAN (Phyllantus niruri L.) PADA TIKUS WISTAR (Rattus norvegicus) JANTAN YANG DIINDUKSI VAKSIN DPT-HB

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Ivana Jansen ◽  
Jane Wuisan ◽  
Henoch Awaloei
Keyword(s):  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Phyllanthus Niruri ◽  
Antipyretic Effect ◽  
Flower Extract ◽  
Hb Vaccine

Abstract: The aim of this study is to investigate the antipyretic effect of leaf-flower extract (Phyllanthus niruri L.) in Wistar rats induced by DPT-HB vaccine. Samples were 15 Wistar rats as test animals and were divided into five groups of three in each. The negative control group was given orally distilled water, positive control group was given paracetamol and 3 experimental groups were given ethanolic leaf-flower extracts (100 mg, 200 mg and 300 mg /200 g body weight of rats). Pyrexia was induced in rats by intramuscular injection of 0.2 ml DPT-HB vaccine. Antipyretic activity was measured at 0, 30, 60, 90, 120, 150, 180 minutes after administration of leaf-flower extract, paracetamol and distilled water. The results showed that leaf-flower extract at a dose of 300 mg /200 g BW decreased the rectal temperature greater than with doses of 100 and 200 mg /200 g BW for 180 minutes of measurement. Conclusion: Leaf-flower extract has antipyretic effect in Wistar rats.Keywords: antipyretic, phyllanthus niruri, DPT-HB, wistar ratAbstrak: Penelitian ini bertujuan untuk menguji efek antipiretik ekstrak meniran (Phyllanthus niruri L.) pada tikus Wistar yang diinduksi vaksin DPT-HB. Metode: Hewan uji yang digunakan adalah 15 ekor tikus Wistar yang dibagi ke dalam 5 kelompok, masing-masing kelompok terdiri dari 3 ekor tikus. Kelompok kontrol negatif diberikan aquades, kelompok kontrol positif diberikan parasetamol, dan 3 kelompok eksperimental diberikan ekstrak tanaman meniran masing-masing dengan dosis 100mg, 200mg dan 300 mg/200 grBB tikus. Induksi demam pada hewan uji menggunakan vaksin DPT-HB 0,2ml secara intramuskular. Pengukuran suhu rektal dilakukan sebelum dan sesudah pemberian vaksin dan setelah pemberian bahan uji yaitu pada menit ke-30, 60, 90, 120, 150 dan 180. Hasil penelitian memperlihatkan pemberian ekstrak meniran dengan dosis 300 mg/200 grBB menunjukkan penurunan suhu rektal lebih besar dibanding dengan dosis 100 dan 200 mg/200 grBB selama 180 menit pengukuran. Simpulan: Ekstrak meniran memiliki efek antipiretik pada tikus Wistar.Kata kunci: antipiretik, phyllanthus niruri, DPT-HB, tikus wistar

Ononis natrix L. Lowers the Blood Glucose Concentration in Wistar Rats with Streptozotocin-induced Diabetes Mellitus

2020 ◽  
Vol 20 ◽  
Author(s):  
Baker F. Mubideen ◽  
Ala-Aldeen Ahmad Al-Serhan ◽  
Justin Z. Amarin ◽  
Arwa Al-Dweikat ◽  
Ra'ad Z. Al-Muhaisen ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Concentration ◽  
Wistar Rats ◽  
Blood Glucose Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Dose Treatment ◽  
Streptozotocin Induced Diabetes

Background: Practitioners of traditional medicine use the decoction of Ononis natrix L. to treat hyperglycemia. The literature offers no evidence to support the use. Objective: To investigate the effect of the decoction of Ononis natrix L. on the blood glucose concentration in Wistar rats (Rattus norvegicus) with streptozotocin-induced diabetes mellitus. Methods: We obtained 35 Wistar rats from the animal colony of The University of Jordan School of Medicine. We induced diabetes by a single intraperitoneal injection of streptozotocin (60 mg/kg body weight) and 23 rats (66%) survived to allocation. We randomly assigned the rats to one of four groups: negative control (1% Tween 80 in distilled water), positive control (100 mg/kg metformin), high-dose treatment (7.5 mL of the decoction), and low-dose treatment (3.5 mL of the decoc-tion). We administered the doses twice daily by oral gavage for two weeks and measured the tail-blood glucose concentration twice daily, once before the first dose and another time after the second dose. We used linear mixed-effects regression to model the change in blood glucose concentration as a function of the experimentation groups, with adjustments for pseu-doreplication and temporal variation. Results: The estimated mean change was 1 mmol/L (−30 to 31 mmol/L) for the negative control group, −26 mmol/L (−56 to 5 mmol/L) for the positive control group, −75 mmol/L (−108 to −42) for the low-dose treatment group, and −82 mmol/L (−111 to −53 mmol/L) for the high-dose treatment group. Conclusion: In conclusion, we demonstrate, for the first time, the hypoglycemic effect of Ononis natrix L. in an animal model of diabetes.

Hepatoprotective effect of methanol seed extract of citrus tangerina on paracetamol-induced hepatotoxicity in Wistar rats

2020 ◽  
Vol 23 ◽  
pp. 83-87
Author(s):  
E.G. Moke ◽  
K.K. Anachuna ◽  
K.E. Edje ◽  
M.O. Ojezele
Keyword(s):  
Wistar Rats ◽  
Liver Enzymes ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Hepatoprotective Effect ◽  
Control Group ◽  
Standard Drug ◽  
Histopathological Studies

This study investigated the hepatoprotective effect of methanol seed extract of Citrus tangerina on liver damage induced by paracetamol in laboratory rats. Wistar rats were used in this study and categorized into five groups. Groups 1 and 2 received 10 ml/kg normal saline orally, groups 3 and 4 were administered 200 mg/kg and 400 mg/kg respectively of Citrus tangerina seed extract orally, while silymarin 100 mg/kg served as standard drug treatment for group 5. Following six (6) days of pretreatment with the extract, hepatotoxicity was induced with paracetamol 3 g/kg (orally) in all the groups except the positive control group. At the end of the experiment (24 hours after induction), blood samples were collected under diethyl ether anaesthesia for biochemical markers of liver enzymes and antioxidative stress and the liver was harvested for histopathological studies. Both doses (200 mg/kg and 400 mg/kg) of Citrus tangerina seed extract significantly (p < 0.05) reduced the liver enzymes level, but significantly (p < 0.05) increased antioxidant enzymes when compared with the negative control group. Liver histology showed that the Citrus tangerina seed extract prevented hepatic injury induced by paracetamol. The methanol seed extract of Citrus tangerina possesses antioxidative and hepatoprotective effects.

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