scholarly journals Higher immunoglobulin production in conjugated linoleic acid-supplemented rats during gestation and suckling

2009 ◽  
Vol 102 (6) ◽  
pp. 858-868 ◽  
Author(s):  
Carolina Ramírez-Santana ◽  
Francisco J. Pérez-Cano ◽  
Cristina Castellote ◽  
Margarida Castell ◽  
Montserrat Rivero ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Oral Administration ◽  
Conjugated Linoleic Acid ◽  
Wistar Rats ◽  
Reference Group ◽  
Standard Diet ◽  
Group A ◽  
Group D

Conjugated linoleic acid (CLA) has been reported to exert beneficial physiological effects on body composition and the immune system. However, little information is available on the influence of CLA on immune function during early life periods. The present study evaluates the effect of feeding an 80:20 mixture of cis-9, trans-11- and trans-10, cis-12-CLA isomers during gestation and suckling on the systemic immune response of weaned Wistar rats. Pups received dietary CLA from dams through the placental barrier and during suckling by breast milk (group A) or by oral administration (group B). Pups from group C only received CLA during suckling by oral administration. Group D constituted the reference group. Milk from dams fed the CLA diet had a high content of CLA and higher IgA and IgG concentrations than rats fed the standard diet. The plasma of pups from groups A, B and C showed six, twelve and nine times higher content of the cis-9, trans-11-CLA isomer than that of the group D pups. Rats from group A exhibited higher serum IgG concentrations than rats from the rest of the groups (22·14 (sem 2·14) v. about 5 mg/ml; P < 0·05), whereas rats from groups A and B showed approximately 2-fold higher splenocyte IgM production than rats from groups C and D. However, CLA supplementation did not influence significantly the splenocyte proliferative response or cytokine secretion. Supplementation during gestation and suckling with an 80:20 cis-9, trans-11–trans-10, cis-12 CLA mix enhances the production of the main in vivo and in vitro Ig isotypes in Wistar rats.

scholarly journals Mining the Microbiota of the Neonatal Gastrointestinal Tract for Conjugated Linoleic Acid-Producing Bifidobacteria

2004 ◽  
Vol 70 (8) ◽  
pp. 4635-4641 ◽  
Author(s):  
E. Rosberg-Cody ◽  
R. P. Ross ◽  
S. Hussey ◽  
C. A. Ryan ◽  
B. P. Murphy ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Bifidobacterium Bifidum ◽  
Genomic Diversity ◽  
Gas Liquid Chromatography ◽  
Fecal Material ◽  
Single Strain ◽  
Different Strains

ABSTRACT This study was designed to isolate different strains of the genus Bifidobacterium from the fecal material of neonates and to assess their ability to produce the cis-9, trans-11 conjugated linoleic acid (CLA) isomer from free linoleic acid. Fecal material was collected from 24 neonates aged between 3 days and 2 months in a neonatal unit (Erinville Hospital, Cork, Ireland). A total of 46 isolates from six neonates were confirmed to be Bifidobacterium species based on a combination of the fructose-6-phosphate phosphoketolase assay, RAPD [random(ly) amplified polymorphic DNA] PCR, pulsed-field gel electrophoresis (PFGE), and partial 16S ribosomal DNA sequencing. Interestingly, only 1 of the 11 neonates that had received antibiotic treatment produced bifidobacteria. PFGE after genomic digestion with the restriction enzyme XbaI demonstrated that the bifidobacteria population displayed considerable genomic diversity among the neonates, with each containing between one and five dominant strains, whereas 11 different macro restriction patterns were obtained. In only one case did a single strain appear in two neonates. All genetically distinct strains were then screened for CLA production after 72 h of incubation with 0.5 mg of free linoleic acid ml−1 by using gas-liquid chromatography. The most efficient producers belonged to the species Bifidobacterium breve, of which two different strains converted 29 and 27% of the free linoleic acid to the cis-9, trans-11 isomer per microgram of dry cells, respectively. In addition, a strain of Bifidobacterium bifidum showed a conversion rate of 18%/μg dry cells. The ability of some Bifidobacterium strains to produce CLA could be another human health-promoting property linked to members of the genus, given that this metabolite has demonstrated anticarcinogenic activity in vitro and in vivo.

scholarly journals The link between supplementary tannin level and conjugated linoleic acid (CLA) formation in ruminants: A meta-analysis

2019 ◽  
Author(s):  
Rayudika Aprilia Patindra Purba ◽  
Pramote Paengkoum ◽  
Siwaporn Paengkoum
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Human Health ◽  
Meta Analysis ◽  
Problem Solver ◽  
Health Issues ◽  
Dietary Treatments

AbstractThis meta-analysis was conducted to predict and assert a way to discover conjugated linoleic acid (CLA) formation in ruminant-derived products as problem solver of human health issues threated by plant-containing tannins. The objective was to expound, to compare, and to confirm the efficiency of tannins cultivating CLA formation whether using in vitro and/or in vivo study. A database was created using the ruminants with selectively 26 experiments comprising 683 dietary treatments as explained in vitro and in vivo methods that were applied as a statistical SAS 9.4 tool. Basically, increasing level of tannins leaded to an underlying decrease in CLA formation (p<0.001), initially at predicting coefficient determination R2=0.193, R2=0.929, and R2=0.549 for CLA in vitro, in vivo of CLA milk shift, and in vivo of CLA meat precipitation, respectively. In vitro may accurately predict to the in vivo observation. Unfortunately, there were no relationship in vitro towards in vivo observation (R2<0.1). It indicated to be difficult to predict CLA from in vitro to in vivo separately situations. According to all studies, the level of tannin’s utilization for inhibiting biohydrogenation was not exceedingly >50 g/kg DM recommended. Secondly, the in vivo method was more suitable for directly observation that concerned in fatty acid transformation.

Synergistic anti-tumor activity of paclitaxel-incorporated conjugated linoleic acid-coupled poloxamer thermosensitive hydrogel in vitro and in vivo

Biomaterials ◽  
2009 ◽  
Vol 30 (27) ◽  
pp. 4777-4785 ◽  
Author(s):  
Ding-Ding Guo ◽  
Cheng-Xiong Xu ◽  
Ji-Shan Quan ◽  
Chung-Kil Song ◽  
Hua Jin ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thermosensitive Hydrogel ◽  
Anti Tumor Activity

scholarly journals In Vivo Study on Site of Action of Sinapine Thiocyanate following Acupoint Herbal Patching

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Shan Chen ◽  
Yu-Tong Jin ◽  
Zheng-Yang Zhu ◽  
Ling-Tao Wu ◽  
Ping Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Wistar Rats ◽  
Pharmacokinetic Profile ◽  
Time Intervals ◽  
Site Of Action ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
Group D

Objective. To investigate the site of action of sinapine thiocyanate (ST), following acupoint herbal patching (AHP). Methods. Twenty Wistar rats were randomized into five groups (groups A, B, C, D, and E), and all groups received the same AHP in vivo. Skin samples were excised at 2 h, 4 h, 6 h, 10 h, and 26 h after AHP administration from group A to group E separately and the concentrations of ST in the skin were determined using a liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method. A pharmacokinetic profile of ST following AHP was performed at the same time in a group of five Wistar rats to detect plasma levels at the same time intervals. Results. The mean ± SD ST concentrations (ng/ml) at 2 h (group A), 4 h (group B), 6 h (group C), 10 h (group D), and 26 h (group E) after AHP administration were 250.01±61.99, 61.01±30.41, 40.12±26.94, 78.66±59.43, and 19.55±18.95, respectively. No ST was detected in rats’ plasma samples at the same time points. Conclusions. The site of action of ST following AHP is in the skin.

scholarly journals Effects of conjugated linoleic acid on proliferation and differentiation of bovine intramuscular preadipocyte in vitro

2021 ◽  
Author(s):  
Rong Wan ◽  
Qingxiang Meng ◽  
Zhou Zhenming ◽  
Wu hao
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Proliferation And Differentiation ◽  
Cell Proportion ◽  
Intramuscular Fat Content

ABSTRACTConjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to increase intramuscular fat content in vivo and stimulate adipogenesis in intramuscular preadipocytes in vitro in pig. Unfortunately, there is little data to evaluate the effect of CLA on proliferation and differentiation of bovine intramuscular preadipocytes. This study investigated the regulation by CLA in proliferation and differentiation of bovine intramuscular preadipocytes. The results demonstrated that CLA significantly induced the expression of PPARγ and C/EBPα mRNA of bovine intramuscular preadipocytes as well as the accumulation of lipid in cultured intramuscular preadipocytes. Additionally, CLA significantly decreased the cell proportion of phase G0/G1, and remarkably increased the proportion of phase S+G2/M. Collectively, these results suggest that CLA promotes bovine intramuscular preadipocyte proliferation and differentiation.

Ameliorative effects of Lactobacillus against Aflatoxin B1

2024 ◽  
Vol 84 ◽  
Author(s):  
R. Yasmeen ◽  
B. Zahid ◽  
S. Alyas ◽  
R. Akhtar ◽  
N. Zahra ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Aqueous Environment ◽  
Serum Total Protein ◽  
Ameliorative Effect ◽  
Group A ◽  
Cell Fractions ◽  
Group D ◽  
Better Than

Abstract Lactobacilli are probiotics with Aflatoxin (AF) detoxification ability, found in fermented products, GIT of animals and environment. Purpose of this study was to investigate the ability of broiler isolates of Lactobacillus against Aflatoxin B1 (AFB1). For this purpose, 5 isolates of Lactobacillus from broiler gut were incubated with 100 ppb AFB1 in aqueous environment and effect of different parameters (cell fractions, time, temperature, pH) on detoxification was determined by HPLC. The ameliorative effect of Lactobacillus salivarius (LS) against AFB1 was studied in broiler. The results revealed that LS (CR. 4) showed the best results (in vitro) as compared to other isolates (L. salivarius (CR. 3, CR, 4), L. agilis (CE. 2.1, CE. 3.1) and L. crispatus (CE. 28). Cell debris of CR. 4 showed significantly higher detoxification (P<0.05). Maximum amount of AFB1 was detoxified at 30°C (97%), pH 4.0 (99%) and 6 h (99.97%). In vivo study showed that AFB1 decreased weight gain (1,269 ± 0.04 gm/ bird), feed consumed (2,161 ± 0.08 gm/ bird), serum total protein (2.42 ± 0.34 gm/ dl), serum albumin (0.5 ± 0.2 2 gm/dl) and antibody titer (4.2 ± 0.83). Liver function enzymes were found (alanine transaminase (ALT): 32 ± 10.7 U/L) and aspartate transaminase (AST): 314.8 ± 27 U/L) elevated in AFB1 fed broilers. Treatment with 1% LS not only decreased the toxic effects of AFB1 (group D) but also improved the overall health of broilers due to its probiotic effects (p<0.05) as compared to control negative (group A). The detoxification ability of LS was better than commercial binder (CB) (0.2% Protmyc). It was concluded that detoxification of AFB1 by Lactobacillus was strain, temperature, pH and time dependent. LS has detoxification ability against AFB1 in vivo.

scholarly journals 10t,12c-conjugated linoleic acid inhibits lipopolysaccharide-induced cyclooxygenase expression in vitro and in vivo

2005 ◽  
Vol 46 (10) ◽  
pp. 2134-2142 ◽  
Author(s):  
Guangming Li ◽  
David Barnes ◽  
Daniel Butz ◽  
Dale Bjorling ◽  
Mark E. Cook
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid

scholarly journals Repair of bone defects with prefabricated vascularized bone grafts and double-labeled bone marrow-derived mesenchymal stem cells in a rat model

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Xiao-Rui Jiang ◽  
Hui-Ying Yang ◽  
Xin-Xin Zhang ◽  
Guo-Dong Lin ◽  
Yong-Chun Meng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Vascular Bundle ◽  
Bone Defects ◽  
Group A ◽  
Group D ◽  
Vascularized Bone

Abstract This study aims to investigate the repair of bone defects with prefabricated vascularized bone grafts and double-labeled bone marrow-derived mesenchymal stem cells (BMSCs) in a rat model. BMSCs were separated from rat bone marrow. LTR-CMVpro-RFP and LTR-CMVpro-GFP were transfected into the BMSCs for in vitro and in vivo tracking. BMSCs-RFP and BMSCs-GFP were induced into endothelial progenitor cells (EPCs) and osteoblasts (OBs). Rats were divided into five groups: Group A: in vitro prefabrication with EPCs-RFP + in vivo prefabrication with arteriovenous vascular bundle + secondary OBs-GFP implantation; Group B: in vitro prefabrication with EPCs-RFP + secondary OBs-GFP implantation; Group C: in vivo prefabrication with arteriovenous vascular bundle + secondary OBs-GFP implantation; Group D: implantation of EPCs-RFP + implantation of with arteriovenous vascular bundle + simultaneous OBs-GFP implantation; Group E: demineralized bone matrix (DBM) grafts (blank control). Among five groups, Group A had the fastest bone regeneration and repair, and the regenerated bone highly resembled normal bone tissues; Group D also had fast bone repair, but the repair was slightly slower than Group A. Therefore, in vitro prefabrication with EPCs-RFP plus in vivo prefabrication with arteriovenous vascular bundle and secondary OBs-GFP implantation could be the best treatment for bone defect.

Influence of conjugated linoleic acid isomers on the metastasis of colon cancer cells in vitro and in vivo

2007 ◽  
Vol 18 (10) ◽  
pp. 650-657 ◽  
Author(s):  
So Mi Soel ◽  
Ok Sook Choi ◽  
Myung Hee Bang ◽  
Jung Han Yoon Park ◽  
Woo Kyoung Kim
Keyword(s):  
Colon Cancer ◽  
Linoleic Acid ◽  
Cancer Cells ◽  
Conjugated Linoleic Acid ◽  
Colon Cancer Cells

138 COMPARISON OF KINETICS AND PATTERNS OF THE FIRST CLEAVAGE OF IN VIVO AND IN VITRO-MATURED HOLSTEIN OOCYTES AFTER IN VITRO FERTILIZATION WITH X-SORTED SPERM

2014 ◽  
Vol 26 (1) ◽  
pp. 182
Author(s):  
S. Matoba ◽  
S. Sugimura ◽  
H. Matsuda ◽  
Y. Aikawa ◽  
M. Ohtake ◽  
...  
Keyword(s):  
Calf Serum ◽  
Time Lapse ◽  
Developmental Competence ◽  
High Rate ◽  
Holstein Cows ◽  
Group A ◽  
Group B ◽  
Group D

Previously, it was reported that a high rate of good quality blastocysts were produced by IVF of in vivo-matured oocytes, obtained by ovum pick up (OPU) after superstimulation in Holstein cows, using X-sorted sperm (Matoba et al. 2012 Reprod. Domest. Anim. 47, 515). In this system, an early first cleavage within 28 h after IVF was found to be a potent marker for the selection of embryos with high developmental competence (Matoba et al. 2013 Reprod. Fertil. Dev. 25, 266). However, we have limited knowledge on the timing and normality of embryonic cleavages in in vitro-matured oocytes after IVF. The purpose of the present study was to compare the kinetics and patterns of the first cleavage of in vivo- and in vitro-matured bovine oocytes after IVF with X-sorted sperm. In vivo-matured oocytes (Group A) were collected by OPU from non-lactating Holstein cows just before ovulation after superstimulation. Immature oocytes were either collected by OPU without hormonal treatment or by aspiration of ovaries at the local abattoir and matured in vitro (Group B or C). All the oocytes were inseminated with 5 × 106 sperm mL–1 of X-sorted sperm, except half of oocytes in Group C inseminated by non-sorted sperm (Group D) and cultured in CR1aa supplemented with 5% calf serum and 0.25 mg mL–1 of linoleic acid albumin at 38.5°C in 5% CO2, 5% O2, and 90% N2 for 216 h. Embryo kinetics were observed individually using a microwell culture dish and time-lapse cinematography (Sugimura et al. 2010 Biol. Reprod. 83, 970–978). Photographs of each embryo were taken in every 15 min during the IVC period and analysed by time-lapse cinematography software. Cleavage pattern was categorized as normal (2 even blastomeres without fragment or protrusion) or abnormal (2 uneven blastomeres, with fragment or protrusion and those dividing into 3–5 blastomeres) at the first cleavage. Data were analysed by ANOVA, chi-squared, or discriminant function. A total of 268 cleaved embryos were used. The blastocyst rate in Group A was higher than in Groups B and C (61.3 v. 40.0 and 25.0%, respectively; P < 0.05). The timing of first cleavage was longer in Group A compared with Groups C and D (28.3 ± 3.8 v. 27.6 ± 3.8 and 26.7 ± 1.9 h, respectively) and in Group B (28.1 ± 4.0 h) compared with in Group D (P < 0.05). Higher rates of normal cleavage were observed in Groups A, B, and D than in Group C (53.5, 44.4, and 54.8 v. 16.7%, respectively; P < 0.01). The frequency of blastocysts derived from the early (28.3 h) and normal pattern cleaving oocytes were greater in Groups A and B than in Group C (29.0 and 20.0 v. 8.3%, respectively; P < 0.05) and similar in Group D (22.6%). Our results reveal that IVF embryos produced from in vivo-matured oocytes with sex-sorted sperm had superior normality than those produced from in vitro-matured oocytes and similar normality to embryos inseminated with non-sorted sperm. Supported by the Research and Development projects for application in promoting new policy of agriculture, forestry and fisheries (22016) and by JSPS and HAS under the Japan-Hungary Research Cooperative Program.

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