Differences in the concentrations of certain blood constituents among cows in a dairy herd

1976 ◽  
Vol 86 (1) ◽  
pp. 171-179 ◽  
Author(s):  
Barbara A. Kitchenham ◽  
G. J. Rowlands
Keyword(s):  
Dairy Cows ◽  
Total Protein ◽  
Inorganic Phosphate ◽  
Dairy Herd ◽  
Blood Samples ◽  
Frequency Distributions ◽  
Blood Constituents ◽  
Stage Of Lactation ◽  
Significant Relationships ◽  
Volume Blood

SUMMARYBlood samples were taken on six occasions from a herd of 172 Friesian, Ayrshire and Friesian × Ayrshire dairy cows, and analysed for packed cell volume, blood glucose, haemoglobin and serum albumin, total protein, urea nitrogen, inorganic phosphate, Ca, Mg, K and Na. Differences in blood composition among cows, adjusted for differences in stage of lactation, were demonstrated for all blood constituents (P < 0·001) with the variation among cows proportionally largest for globulin and total protein and smallest for Na. Correlations between blood constituents were for the most part small.Globulin and total protein concentrations increased with age (P < 0·001) and concentrations of inorganic phosphate, albumin, Mg, Na and urea decreased with increasing age (P < 0·001 for inorganic phosphate and Mg, P < 0·01 for albumin, Na and urea). Frequency distributions of the concentrations of the blood constituents adjusted for age and breed showed significant deviations from normality for globulin (P < 0·05) and Hb (P < 0·01).There were significant relationships between the concentrations of globulin and total protein of 43 dams and their daughters (P < 0·01 for globulin, P < 0·05 for total protein). There were also significant differences in groups of daughters of different sires for concentrations of urea, globulin, total protein, albumin, Mg, Ca, K and PCV (P < 0·01 for urea and globulin, P < 0·05 for the other constituents).

Relationships between blood composition and fertility in dairy cows – a field study

1977 ◽  
Vol 44 (1) ◽  
pp. 1-7 ◽  
Author(s):  
G. J. Rowlands ◽  
W. Little ◽  
B. A. Kitchenham
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Total Protein ◽  
Inorganic Phosphate ◽  
Metabolic Profile ◽  
Serum Urea ◽  
Blood Samples ◽  
Stage Of Lactation ◽  
Lactating Dairy Cows ◽  
The Difference

SummaryThree hundred and fifty-one lactating dairy cows, which were between 40 and 100 d post-calving, were selected from cows sampled during metabolic profile testing of 21 herds. Blood samples were analysed for packed cell volume (PCV), blood glucose and haemoglobin, and serum urea-N, albumin, total protein, inorganic phosphate, Ca, Mg, Na, K, Cu and Fe. After adjusting for herd and date of sampling and each cow's age, stage of lactation, milk yield and interval from calving to first service, concentrations of albumin and K (P < 0·01) and PCV (P < 0·05) were inversely related and concentrations of globulin (derived as the difference between total protein and albumin) directly related (P < 0·05) to the number of services required for conception. Conception rates to first service were inversely related to lactation number but not to milk yield on day of sampling.

Relationships between stage of lactation and pregnancy and blood composition in a herd of dairy cows and the influences of seasonal changes in management on these relationships

1975 ◽  
Vol 42 (3) ◽  
pp. 349-362 ◽  
Author(s):  
G. J. Rowlands ◽  
R. Manston ◽  
Rita M. Pocock ◽  
Sally M. Dew
Keyword(s):  
Milk Yield ◽  
Inorganic Phosphate ◽  
Post Partum ◽  
Dairy Herd ◽  
Late Pregnancy ◽  
Early Lactation ◽  
Lactating Cows ◽  
Stage Of Lactation ◽  
Volume Blood ◽  
Selection Of

SummaryBlood samples, taken on 6 occasions during 1 year from 172 cows resident in a dairy herd, were analysed for packed cell volume, blood glucose, haemoglobin (Hb), serum albumin, urea N, total protein, Ca, inorganic phosphate, Mg, Na and K. The data were analysed statistically, firstly with respect to date of sampling, stage of pregnancy or lactation and the interaction between stage of pregnancy or lactation and date of sampling, and secondly with milk yield as an additional factor for the data for lactating cows.Concentrations of all constituents except inorganic phosphate and K varied significantly (P<0·001) with stage of lactation and/or pregnancy. The most significant changes in concentrations were confined to the periods up to 3 months either side of calving. The greatest changes in concentration during these periods occurred for Mg which rose during late pregnancy and for albumin which fell at or near calving. Albumin, urea and glucose were all, on average, lowest in concentration during the first month of lactation; globulin concentrations showed the reverse trend. Haemoglobin concentrations decreased during late pregnancy and early lactation and were lowest in the period 30–120 d post partum. Calcium concentrations decreased during late pregnancy and rose during early lactation. Sodium concentrations rose during late pregnancy. Interactions between dates of sampling and stages of lactation or pregnancy were significant (P<0·001) for all constituents, and most marked for glucose, urea, albumin, Hb, Ca and Na.Haemoglobin and K concentrations fell and Mg concentrations rose with increasing milk yield, but the proportions of the total variance accounted for by differences in milk yield were small in relation to those accounted for by stage of lactation. These findings are of significance in relation to the selection of animals for sampling in the Compton Metabolic Profile Test.

Effects of feeding peas to high-producing dairy cows

1995 ◽  
Vol 75 (4) ◽  
pp. 625-629 ◽  
Author(s):  
R. R. Corbett ◽  
L. A. Goonewardene ◽  
E. K. Okine
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Dairy Herd ◽  
Protein Source ◽  
Canola Meal ◽  
Stage Of Lactation ◽  
Nutrient Analyses ◽  
Protein Milk

The effect of substituting peas for soybean and canola meals as a protein source in a high-producing dairy herd was studied in 66 Holstein cows, divided into two groups based on stage of lactation, parity, level of milk production and days in milk. Two 18.5% crude protein grain concentrate diets were formulated based on the nutrient analyses of the forages available. The control grain mix contained standard protein sources, principally soybean and canola meal (SBM\CM) while the test grain mix was formulated to contain approximately 25% field peas as the major source of protein. Both grain rations were formulated to the same nutrient specifications and balanced for undegradable protein. The duration of the trial was 6 mo during which grain feeding levels were adjusted monthly based on milk yield. For cows in early lactation, 4% fat-corrected milk yield was higher (P < 0.05) for cows fed pea based concentrates (31.3 kg d−1) than for cows fed SBM\CM supplement (29.7 kg d−1). Fat-corrected milk yield was not affected by source of protein in mid- and late-lactation cows. Fat-corrected milk production was not different (P > 0.05) for cows fed SBM\CM compared with cows fed the pea supplement when cows across all stages of lactation were included in the analyses. Milk fat percent was significantly higher (P < 0.05) for early- and mid-lactation cows fed the pea supplement. The results suggest that peas can be substituted for SBM\CM as a protein source for high-producing dairy cows. Key words: Dairy cow, pea, soybean and canola meal supplement, undegradable protein, milk production

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

Relationship between the blood composition of Friesian (Holstein) bulls and their improved contemporary comparisons for production traits

1986 ◽  
Vol 107 (1) ◽  
pp. 71-74
Author(s):  
G. J. Rowlands ◽  
Kathryn J. Bunch ◽  
P. A. Brookes ◽  
R. Manston
Keyword(s):  
Blood Glucose ◽  
Milk Production ◽  
Inorganic Phosphate ◽  
Production Traits ◽  
Production Potential ◽  
Blood Concentrations ◽  
Blood Samples ◽  
Blood Constituents ◽  
Yield And Quality ◽  
Holstein Bulls

SUMMARYBlood samples were taken from 428 Friesian (Holstein) bulls on three occasions, on average at approximately 3-monthly intervals, between 3 and 15 months of age, and also from 214 of them on three occasions at 6-monthly intervals when they were between 2 and 6 years of age.The average concentrations of blood glucose and haemoglobin and of serum cholesterol, urea, albumin, globulin, inorganic phosphate, Ca, Mg, Na, K and Cu were calculated for each of these periods and correlated with improved contemporary comparisons of milk yield and quality.Repeatability estimates were similar within each of the two periods but were lower when mean concentrations calculated between 3 and 15 months of age were compared with those between 2 and 6 years. The main exceptions were cholesterol (0·52) and Cu (0·54) for which repeatability estimates were similar to those derived within the above two periods. The constituents with the next highest levels of repeatability were magnesium (0·44), globulin (0·43) and inorganic phosphate (0·39), which were approximately 30% lower than those obtained within the two periods.Correlations between blood concentrations and improved contemporary comparisons were negligible, indicating that it is not possible to use these blood constituents to predict the milk production potential of a bull's daughters.

Effects of some anti-inflammatory drugs on 12 blood constituents: protocol for the study of in vivo effects of drugs.

1985 ◽  
Vol 31 (7) ◽  
pp. 1141-1143 ◽  
Author(s):  
Z Jelić-Ivanović ◽  
S Spasić ◽  
N Majkić-Singh ◽  
P Todorović
Keyword(s):  
Uric Acid ◽  
Acetylsalicylic Acid ◽  
Total Protein ◽  
Inorganic Phosphate ◽  
Drug Effects ◽  
Urea Nitrogen ◽  
Blood Constituents ◽  
Inflammatory Drugs ◽  
In Vivo Effects

Abstract We investigated the in vivo effects of acetylsalicylic acid, diclofenac, indomethacin, ibuprofen, and ketoprofen on the concentrations of various blood constituents. Total protein, glucose, calcium, and inorganic phosphate were not significantly affected by any of these drugs. Ketoprofen had no definite influence on any constituent. Acetylsalicylic acid induced an increase in cholesterol, triglyceride, and iron; albumin, uric acid, and creatinine decreased with ibuprofen therapy. Urea nitrogen increased in patients treated with diclofenac or indomethacin. Our protocol for the study of in vivo drug effects is discussed.

The blood composition of Friesian bulls; variations with age and relationships with results of progeny tests

1978 ◽  
Vol 91 (3) ◽  
pp. 579-585 ◽  
Author(s):  
A. J. Stark ◽  
G. J. Rowlands ◽  
R. Manston ◽  
A. E. McClintock
Keyword(s):  
Dairy Cows ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Inorganic Phosphate ◽  
Mean Corpuscular Volume ◽  
Cattle Breeding ◽  
Blood Samples ◽  
Progeny Tests ◽  
Milk Marketing ◽  
Cell Volumes

SummaryBlood samples were taken from 172 Friesian bulls during 1973–5 on three or four occasions. The bulls were in Milk Marketing Board cattle breeding centres in England and Wales and were from 1 to 14 years of age.The blood samples were analysed for packed cell volume, haemoglobin, erythrocytes, mean corpuscular volume and glucose, and samples of serum for the concentrations of albumin, total protein, urea-nitrogen, inorganic phosphate, Ca, Mg, K, Na and Cu.Packed cell volumes and haemoglobin and K concentrations were higher in bulls than those previously recorded in dairy cows. There were significant age relationships for packed cell volume, haemoglobin, albumin and globulin (P < 0·001), inorganic phosphate and erythrocytes (P < 0·01) and Ca and Mg (P < 0·05).Repeatability estimates were calculated and compared with estimates previously obtained for dairy cows.On the basis of improved contemporary comparisons of the bulls, there was a significant relationship between the average milk yield of a bulls daughters and the concentrations of urea, inorganic phosphate and K (P < 0·05) in the blood serum of the bull.

Individuality and heritability of the blood composition of calves with particular reference to the selection of stock with improved growth potential

1974 ◽  
Vol 82 (3) ◽  
pp. 473-481 ◽  
Author(s):  
G. J. Rowlands ◽  
J. M. Payne ◽  
Sally M. Dew ◽  
R. Manston
Keyword(s):  
Growth Rate ◽  
Inorganic Phosphate ◽  
Blood Analysis ◽  
Growth Potential ◽  
Blood Composition ◽  
Blood Constituents ◽  
Body Weights ◽  
Volume Blood ◽  
Blood Characteristics ◽  
Selection Of

SUMMARYBlood samples, taken from each of 231 calves on three occasions at 9,10 and 11 weeks of age, were analysed for packed cell volume, blood glucose and Hb; and serum albumin, urea nitrogen, total protein, Ca, inorganic phosphate, Mg, K, Na and Cu. Differences in the blood composition for individual calves were demonstrated for all blood constituents (P < 0·01) with variations among calves proportionally largest for albumin and smallest for sodium. Concentrations of glucose, Hb and K were inherited (heritability estimates > 0·4).There were significant correlations (P < 0·001) between the calves' growth rates from 1 to 12 weeks and the concentrations of each of the blood constituents, glucose Hb, K, Na, albumin and inorganic phosphate; in the cases of Na and albumin, comparison of the blood analysis at 9–11 weeks with body weights at 6 and 9 months showed that these correlations persisted, and that by 9 months differences in concentrations of Na and albumin among individuals were associated with anaveragedifference of as much as 55 kg in body weight.The evidence that concentrations of certain blood constituents are both inherited and related to growth rate suggests that not only might calves be screened early in life for indications of rapid growth rate, but also that animals might be bred to have the blood characteristics indicative of this quality.

scholarly journals Relationship among blood metabolites and lipid content in the liver in transitional dairy cows

2012 ◽  
Vol 28 (4) ◽  
pp. 705-714 ◽  
Author(s):  
R. Djokovic ◽  
H. Samanc ◽  
M.D. Petrovic ◽  
Z. Ilic ◽  
V. Kurcubic
Keyword(s):  
Dairy Cows ◽  
Total Cholesterol ◽  
Lipid Content ◽  
Total Bilirubin ◽  
Transition Period ◽  
Dairy Herd ◽  
Hepatic Function ◽  
Transitional Period ◽  
Blood Samples

The objective of the present study was to determine a relationship among blood indicators of hepatic function and lipid content in the liver during transitional period in dairy cows. Late pregnant and calved cows (n= 40) were selected from a Holstein dairy herd and allocated to four groups: a late pregnant cows (n=10) from day 15 to day 5 before calving; late pregnant cows (n=10) from day 5 to day 1 before calving; clinically puerperal healthy cows (n=10) and clinically ketotic puerperal cows (n=10). Liver and blood samples were taken from all cows. The results of present investigation have shown that the lipid content in the liver and the blood non-esterificed fatty acids, ?-hydroxybutyrate, total bilirubin concentrations and the AST activities were significantly higher (P<0.05) as well the blood glucose, total cholesterol, triglycerides and albumin concentrations were significantly lower (P<0.05) in puerperal ketotic cows as compared to the values of these parameters in the blood of healthy cows. The fat proportions in liver were positively (P<0.05) associated with the AST activities and with the serum NEFA and BHB concentrations, but negatively correlated (P<0.05) with the circulating triglyceride, total cholesterol and albumin concentrations, All these biochemical metabolites may be used as important biochemical indicators in the determination of the functional status of the liver in dairy cows during the transition period.

scholarly journals Metabolic Profile and Hormonal Status Comparison Between Primiparous and Multiparous Non-Cyclic Cows

2018 ◽  
Vol 41 (2) ◽  
pp. 195-201 ◽  
Author(s):  
Besir Jasari ◽  
Branko Atanasov ◽  
Irena Celeska ◽  
Miroslav Radeski ◽  
Martin Nikolovski ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Total Protein ◽  
Metabolic Profile ◽  
Transrectal Ultrasonography ◽  
Hormonal Status ◽  
Metabolic Profiles ◽  
Serum Concentrations ◽  
Physiological Concentration ◽  
Blood Samples ◽  
Luteal Tissue

Abstract Several reports indicated that a large proportion of dairy cows have not resumed cyclicity until day 60 after calving. These cows are traditionally classified as non-cycling (anoestrous or anovular cows). Static ovaries (SO, lack of luteal tissue and follicles >8 mm, and progesterone < 0.5 ng/mL) could be a possible underlying reason that contributes to a non-cycling status. Although SO affects both primiparous (PP) and multiparous (MP) cows, PP cows are more prone to be non-cycling than MP. Therefore, this study aims to compare the metabolic profiles and hormonal status between non-cycling PP and MP cows diagnosed with SO. One hundred and twenty one animals that did not express signs of oestrus until day 60 postpartum were grouped by parity (PP, n=58 and MP, n=63), then blood sampled and examined using transrectal ultrasonography. Blood samples were collected before the ultrasonographic examination. Out of those, 42 PP (72.4%) and 28 MP (44.4%) were diagnosed as non-cycling (bearing SO). Serum concentrations of triglycerides, cholesterol, total protein and albumin did not differ between parity groups. The glucose concentrations in PP cows (1.43 ± 0.59 mmol/L) and MP cows (1.69 ± 0.71 mmol/L) did not differ, however, they were less than the normal physiological concentration. In addition, no differences were detected between parity groups for concentrations of NEFA, β-HBA, progesterone and estradiol. In summary, we concluded that non-cycling PP and MP cows bearing SO have similar hormonal status and metabolic profiles.

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