Effects of some anti-inflammatory drugs on 12 blood constituents: protocol for the study of in vivo effects of drugs.

1985 ◽  
Vol 31 (7) ◽  
pp. 1141-1143 ◽  
Author(s):  
Z Jelić-Ivanović ◽  
S Spasić ◽  
N Majkić-Singh ◽  
P Todorović
Keyword(s):  
Uric Acid ◽  
Acetylsalicylic Acid ◽  
Total Protein ◽  
Inorganic Phosphate ◽  
Drug Effects ◽  
Urea Nitrogen ◽  
Blood Constituents ◽  
Inflammatory Drugs ◽  
In Vivo Effects

Abstract We investigated the in vivo effects of acetylsalicylic acid, diclofenac, indomethacin, ibuprofen, and ketoprofen on the concentrations of various blood constituents. Total protein, glucose, calcium, and inorganic phosphate were not significantly affected by any of these drugs. Ketoprofen had no definite influence on any constituent. Acetylsalicylic acid induced an increase in cholesterol, triglyceride, and iron; albumin, uric acid, and creatinine decreased with ibuprofen therapy. Urea nitrogen increased in patients treated with diclofenac or indomethacin. Our protocol for the study of in vivo drug effects is discussed.

scholarly journals Effect of Dietary Extruded Linseed and Walnut Meal Mixture (8:1) on Performance and Plasma Protein Profile in Weaned Piglets

2018 ◽  
Vol 75 (2) ◽  
pp. 121 ◽  
Author(s):  
Anca GHEORGHE ◽  
Mihaela HĂBEANU ◽  
Nicoleta Aurelia LEFTER ◽  
Daniela Mihaela GRIGORE
Keyword(s):  
Uric Acid ◽  
Plasma Protein ◽  
Soybean Meal ◽  
Total Protein ◽  
Total Bilirubin ◽  
Protein Profile ◽  
Weaned Piglets ◽  
Urea Nitrogen ◽  
Blood Samples ◽  
Dietary Nitrogen

The effects of dietary extruded linseed (ELS):walnut meal (WM) mixture (8:1) on performance and plasma protein profile in weaned piglets was evaluated for 21 d. Topigs piglets (n=40; BW=8.02±0.82 kg), age 30±3 days, were allotted into 2 groups and fed 2 diets: control [C, based on corn-triticale-soybean meal (SBM)] and experimental (ELS:WM, where the ELS:WM mixture (8:1) partially replace SBM). Blood samples were collected at 7d and 21d after weaning. The plasma protein profile (total protein, total bilirubin, albumin, creatinine, uric acid, urea nitrogen-BUN) were determined by a chemistry analyser. Dietary ELS:WM mixture improve the BW (P=0.047) and ADG (P=0.036) of piglets at 21d after weaning vs C, whereas ADFI and F:G ratio were increase (P>0.05). The plasma protein profile of piglets fed dietary mixture was not affected at 7d and 21d after weaning, except plasma BUN concentration that was decrease (P=0.027) at 21d after weaning. Lower BUN concentration indicated higher availability of dietary nitrogen reflected in a higher deposition of protein. We concluded that dietary ELS:WM mixture improve piglets performance and positively afect plasma protein profile, especially BUN in weaning period.

Development of Normal Values for Use in Multitest Biochemical Screening of Sera

1970 ◽  
Vol 16 (3) ◽  
pp. 161-165 ◽  
Author(s):  
Richard T O’Kell ◽  
Joseph R Elliott
Keyword(s):  
Uric Acid ◽  
Total Protein ◽  
Protein Concentration ◽  
Normal Values ◽  
Lactic Dehydrogenase ◽  
Uric Acid Concentration ◽  
Total Protein Concentration ◽  
Urea Nitrogen ◽  
Mean Values ◽  
Calcium Phosphorus

Abstract Age- and sex-related "normal" values were calculated for data obtained by screening 8015 patients’ sera with the Technicon SMA 12/60 at the time of hospital admission. Calcium, phosphorus, total protein, and albumin concentrations decrease with age; glucose, urea nitrogen, cholesterol, and lactic dehydrogenase concentrations increase. Uric acid concentration increases with age in women. Mean values for calcium, glucose, urea nitrogen, and uric acid concentrations are greater in men. Serum glutamic-oxaloacetic acid transaminase activity is most frequently abnormal, total protein concentration least frequently. Alkaline phosphatase and bilirubin results were unremarkable.

Assessment of interference by aspirin with some assays commonly done in the clinical laboratory.

1976 ◽  
Vol 22 (6) ◽  
pp. 837-842 ◽  
Author(s):  
J I Routh ◽  
W D Paul
Keyword(s):  
Uric Acid ◽  
Drug Therapy ◽  
Standard Deviation ◽  
Acetylsalicylic Acid ◽  
Total Protein ◽  
Clinical Laboratory ◽  
Aspirin Therapy ◽  
The Difference ◽  
T Values

Abstract We have assessed the interference of acetylsalicylic acid (aspirin) with some common clinical laboratory assays, because of its widespread use and reported interference. The therapeutic regimens involved ingestion of 10 325-mg tablets daily for three days or eight tablets for five days or two weeks. Twenty-one commonly done determinations were run on control sera, and sera were collected during and after drug therapy. Significant changes in t-test values, indicating the significance of the standard deviation of the difference between controls and specimens drawn after drug therapy, were observed for chloride (increased), and for total protein, calcium, cholesterol, uric acid, bilirubin, and thyroxine (decreased). Aspirin therapy depressed the apparent concentrations of these constituents progressively in the three-day regimen and initially in the long-term regimens, followed by recovery toward zero t-values at the end of the longer regimens.

Effects of Indomethacin, Dexamethasone, and Erythromycin on Endotoxin-Induced Intraepithelial Mucus Production of Rat Nasal Epithelium

1997 ◽  
Vol 106 (8) ◽  
pp. 683-687 ◽  
Author(s):  
Yukimitsu Takahashi ◽  
Takeshi Shimizu ◽  
Yasuo Sakakura
Keyword(s):  
Body Weight ◽  
Intraperitoneal Injection ◽  
Goblet Cells ◽  
Inhibitory Effect ◽  
Respiratory Epithelium ◽  
Mucus Production ◽  
Inflammatory Drugs ◽  
In Vivo Effects ◽  
Intranasal Instillation

We produced hypertrophic and metaplastic changes of goblet cells in rat nasal respiratory epithelium by the intranasal instillation of endotoxin (ETN). In the present study, we examined in vivo effects of indomethacin (IND), dexamethasone (DEX), and erythromycin (EM) on intraepithelial mucus production using this animal model. Intraperitoneal injection of IND (2 to 4 mg/kg body weight x 4 days) or DEX (4 to 8 mg/kg body weight x 4 days) significantly inhibited intraepithelial mucus production induced after 3 days of ETN instillations. Intraperitoneal injection of EM (100 mg/kg body weight x 8 days), aminobenzylpenicillin (ABPC, 200 mg/kg body weight x 8 days), and cephalothin (CET, 200 mg/kg body weight x 8 days) also inhibited intraepithelial mucus production induced after 7 days of ETN instillations. When compared with ABPC and CET, EM had a greater inhibitory effect. These results indicate that ETN-induced intraepithelial mucus production can be inhibited by treatment with the anti-inflammatory drugs IND and DEX. Antibiotics such as EM, ABPC, and CET will also be effective, probably by preventing secondary bacterial infection, and EM has an additional inhibitory effect on intraepithelial mucus production.

Ultramicroscale Determination of Clinical Chemical Values for Blood during the First Four Days of Postnatal Life

1975 ◽  
Vol 21 (6) ◽  
pp. 746-750 ◽  
Author(s):  
Larissa deBaare ◽  
Jean Lewis ◽  
Helen Sing
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Total Protein ◽  
Inorganic Phosphorus ◽  
Postnatal Life ◽  
Total Proteins ◽  
Blood Constituents ◽  
Clinical Chemical ◽  
Term Newborns

Abstract Ultramicro procedures requiring 5-10 µl of serum or blood per analysis were used in determining blood constituents of healthy full-term newborns during the first four days of life. The resulting values appeared to be influenced by age, sex, and race. Values for total protein, albumin, urea nitrogen, and uric acid in serum decreased with time; serum inorganic phosphorus and whole-blood aldosaccharoses increased. Serum from females had higher values than that from males for total proteins, albumin, and inorganic phosphorus. The values for serum calcium and alkaline phosphatase were consistently higher in Negro than in white infants; values for uric acid were higher in the latter.

Differences in the concentrations of certain blood constituents among cows in a dairy herd

1976 ◽  
Vol 86 (1) ◽  
pp. 171-179 ◽  
Author(s):  
Barbara A. Kitchenham ◽  
G. J. Rowlands
Keyword(s):  
Dairy Cows ◽  
Total Protein ◽  
Inorganic Phosphate ◽  
Dairy Herd ◽  
Blood Samples ◽  
Frequency Distributions ◽  
Blood Constituents ◽  
Stage Of Lactation ◽  
Significant Relationships ◽  
Volume Blood

SUMMARYBlood samples were taken on six occasions from a herd of 172 Friesian, Ayrshire and Friesian × Ayrshire dairy cows, and analysed for packed cell volume, blood glucose, haemoglobin and serum albumin, total protein, urea nitrogen, inorganic phosphate, Ca, Mg, K and Na. Differences in blood composition among cows, adjusted for differences in stage of lactation, were demonstrated for all blood constituents (P < 0·001) with the variation among cows proportionally largest for globulin and total protein and smallest for Na. Correlations between blood constituents were for the most part small.Globulin and total protein concentrations increased with age (P < 0·001) and concentrations of inorganic phosphate, albumin, Mg, Na and urea decreased with increasing age (P < 0·001 for inorganic phosphate and Mg, P < 0·01 for albumin, Na and urea). Frequency distributions of the concentrations of the blood constituents adjusted for age and breed showed significant deviations from normality for globulin (P < 0·05) and Hb (P < 0·01).There were significant relationships between the concentrations of globulin and total protein of 43 dams and their daughters (P < 0·01 for globulin, P < 0·05 for total protein). There were also significant differences in groups of daughters of different sires for concentrations of urea, globulin, total protein, albumin, Mg, Ca, K and PCV (P < 0·01 for urea and globulin, P < 0·05 for the other constituents).

Effect of nonsteroidal anti-inflammatory drugs on the microsomal monooxygenase system of rat liver

1985 ◽  
Vol 63 (7) ◽  
pp. 798-803 ◽  
Author(s):  
P. M. Bélanger ◽  
A. Atitsé-Gbeassor
Keyword(s):  
Salicylic Acid ◽  
Acetylsalicylic Acid ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Microsomal Monooxygenase ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Cytochrome P 450

The effect of acetylsalicylic acid, ibuprofen, indomethacin, ketoprofen, naproxen, phenylbutazone, and salicylic acid on the microsomal oxidative drug metabolism of rat liver was studied. Pretreatment of the rats with pharmacologic doses of acetylsalicylic acid, indomethacin, and ketoprofen decreased both the demethylase and hydroxylase activities of rat liver microsomes. These effects were paralleled by decreases in microsomal cytochrome P-450 content. The rate of the microsomal reactions was increased after pretreatment with ibuprofen and naproxen but only the former increased the concentration of cytochrome P-450. Phenylbutazone and salicylic acid had no in vivo effect on the hepatic monooxygenase. The addition of 1 mM of ibuprofen, indomethacin, ketoprofen, naproxen, and phenylbutazone to rat liver microsomes inhibit both the aminopyrine N-demethylase and p-nitro-anisole O-demethylase activities. The extent of the inhibition varied between 21 and 73% of the control incubation. Indomethacin, naproxen, and phenylbutazone also decreased the aniline hydroxylase activity to roughly 60% of the control value. Acetylsalicylic acid and salicylic acid had no in vitro effect on the microsomal monooxygenase. The nonsteroidal anti-inflammatory drugs produced a reverse type I binding spectrum with oxidized cytochrome P-450; indomethacin and phenylbutazone were the strongest ligands. There is no correlation between the effect of addition of nonsteroidal anti-inflammatory drugs to the hepatic microsomal homogenate and their in vivo effect on the monooxygenase activity.

scholarly journals Acetylsalicylic acid and labeling of blood constituents with technetium-99m

2005 ◽  
Vol 48 (spe2) ◽  
pp. 163-168 ◽  
Author(s):  
Adenilson de Souza da Fonseca ◽  
Jacques Natan Grinapel Frydman ◽  
Vanessa Câmara da Rocha ◽  
Mario Bernardo-Filho
Keyword(s):  
Acetylsalicylic Acid ◽  
Whole Blood ◽  
Wistar Rats ◽  
Human Beings ◽  
Blood Constituents ◽  
Technetium 99M ◽  
In Vivo Assays ◽  
99Mtc Labeling

Acetylsalicylic acid is the drug most used an anti-inflammatory agent and for secondary prevention of thrombotic phenomenon. Drugs can modify the labeling of blood constituents with technetium-99m (99mTc). The aim of this work was to evaluate the effect of in vitro or in vivo assays with acetylsalicylic acid on the labeling of the blood constituents with 99mTc. In vitro assay was performed with samples of whole blood from Wistar rats incubated with acetylsalicylic acid (1.0 mg/ml) for one hour before the 99mTc-labeling process. For in vivo assay, Wistar rats were treated with acetylsalicylic acid (1.5 mg/kg) during one hour, and the whole blood was withdrawn for the 99mTc-labeling process. Saline was used in control groups. Data showed that the fixation of 99mTc to the blood constituents was not significantly (p>0.05) modified in in vitro and in vivo assays with acetylsalicylic acid, at least not when the experiments were carried out with the doses normally used in human beings.

Sign in / Sign up

Export Citation Format

Share Document