Modification of host–parasite interactions through mutagenesis in quantitative resistance of rice to bacterial leaf blight

1988 ◽  
Vol 111 (2) ◽  
pp. 309-315 ◽  
Author(s):  
H. Nakai ◽  
K. Katsumata ◽  
M. Gohda ◽  
J. Watanabe ◽  
K. Koike
Keyword(s):  
Quantitative Resistance ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Artificial Inoculation ◽  
Induced Mutations ◽  
Host Parasite Interactions ◽  
Group I ◽  
Mutant Lines ◽  
Host Parasite ◽  
Selection Of

SummaryA number of mutant lines showing different levels of improved quantitative resistance to bacterial leaf blight (BLB) were obtained through selection of plants or lines derived from seeds of the rice cultivar Harebare exposed to thermal neutrons and gamma-rays. The selections were made on M2–M4 materials under natural BLB-epidemic conditions and/or by artificial inoculation of one of the BLB isolates. Bacterial isolates predominating under the natural conditions were identified as belonging to the differential BLB isolate group I, and the isolates of this group were used in the artificial inoculation for selection of mutants. The selected M4 mutant lines were tested in M5 for BLB resistance by artificial inoculations of four differential bacterium isolates, groups I, II, III and IV. The M6 mutant lines showed quantitatively elevated resistances to the isolate of bacterium group I and also to the other three differential isolates. Analysis of variance of the data demonstrated that the observed elevated quantitative resistances of the mutants to the isolates I, II, III and IV were isolatespecific. Host-parasite interactions in quantitative resistance to BLB and their modification by induced mutations are discussed.

scholarly journals Response of a local rice variety Pandanwangi to infection of bacterial leaf blight Xanthomonas oryzae pv. oryzae strain III, IV, and VIII

2021 ◽  
Vol 905 (1) ◽  
pp. 012068
Author(s):  
Hadiwiyono ◽  
S H Poromarto ◽  
S Widono ◽  
R F Rizal
Keyword(s):  
Rice Variety ◽  
Leaf Blight ◽  
Rice Production ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Artificial Inoculation ◽  
Limiting Factors ◽  
Bacterial Suspension ◽  
West Java ◽  
Local Cultivar

Abstract Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the limiting factors in rice production. A local cultivar, rice “Pandanwangi” is a superior variety much preferred and cultivated by the farmers in Cianjur, West Java, Indonesia. The information about the response of “Pandangwangi” to Xoo is still poorly understood. This paper reports the results of the evaluation of “Pandanwangi” response against BLB. This research was conducted in a greenhouse with artificial inoculation using Xoo strains III, IV, and VIII with bacterial suspension at 108 cfu.mL−1. The results showed that the response of cv Pandanwangi to Xoo infection was different from the strain of Xoo. “Pandanwangi” cultivar was susceptible to Xoo strain III and VIII and very susceptible to strain IV.

scholarly journals Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0255470
Author(s):  
Phuong Nguyen Duy ◽  
Dai Tran Lan ◽  
Hang Pham Thu ◽  
Huong Phung Thi Thu ◽  
Ha Nguyen Thanh ◽  
...  
Keyword(s):  
Agronomic Traits ◽  
Rice Variety ◽  
Rice Cultivar ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Transcription Activator ◽  
Rice Varieties ◽  
Sugar Transporters ◽  
Blight Disease ◽  
Mutant Lines

TBR225 is one of the most popular commercial rice varieties in Northern Vietnam. However, this variety is highly susceptible to bacterial leaf blight (BLB), a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) which can lead to important yield losses. OsSWEET14 belongs to the SWEET gene family that encodes sugar transporters. Together with other Clade III members, it behaves as a susceptibility (S) gene whose induction by Asian Xoo Transcription-Activator-Like Effectors (TALEs) is absolutely necessary for disease. In this study, we sought to introduce BLB resistance in the TBR225 elite variety. First, two Vietnamese Xoo strains were shown to up-regulate OsSWEET14 upon TBR225 infection. To investigate if this induction is connected with disease susceptibility, nine TBR225 mutant lines with mutations in the AvrXa7, PthXo3 or TalF TALEs DNA target sequences of the OsSWEET14 promoter were obtained using the CRISPR/Cas9 editing system. Genotyping analysis of T0 and T1 individuals showed that mutations were stably inherited. None of the examined agronomic traits of three transgene-free T2 edited lines were significantly different from those of wild-type TBR225. Importantly, one of these T2 lines, harboring the largest homozygous 6-bp deletion, displayed decreased OsSWEET14 expression as well as a significantly reduced susceptibility to a Vietnamese Xoo strains and complete resistance to another one. Our findings indicate that CRISPR/Cas9 editing conferred an improved BLB resistance to a Vietnamese commercial elite rice variety.

Induction and selection of mutations for resistance against bacterial leaf blight in rice

Euphytica ◽  
1985 ◽  
Vol 34 (3) ◽  
pp. 577-585 ◽  
Author(s):  
H. Nakai ◽  
M. Kobayashi ◽  
M. Saito
Keyword(s):  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Selection Of

An approach for breeding varieties of rice resistant to bacterial leaf blight with induced mutations

1975 ◽  
Vol 84 (1) ◽  
pp. 167-172 ◽  
Author(s):  
H. Nakai ◽  
M. Goto
Keyword(s):  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Induced Mutations ◽  
Disease Reaction ◽  
Resistant Varieties ◽  
And Control ◽  
Highly Virulent

SUMMARYThe studies were designed for breeding resistant varieties of rice to bacterial leaf blightcaused by Xanlhomonas oryzae (Uyeda et Ishiyama) Dowson with induced mutations. In 1971, seedlings of irradiated lines (X$) originated from treatments of 60Co γ-and X-radiation and control (C6), which were propagated in bulk up to X6(C6) generations after the treatments, were inoculated with highly virulent isolates.The results showed that the variability of disease reaction within the irradiated lines increased in both resistant and susceptible directions from the control mean. It was assumed from these results that the irradiation treatments could induce polygenic mutations to give both germ types more resistant and those more susceptible to bacterial leaf blight, suggesting that such treatments might be an effective approach for breeding varieties resistant to the disease.

scholarly journals Highly efficient generation of bacterial leaf blight-resistant and transgene-free rice using a genome editing and multiplexed selection system

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Kun Yu ◽  
Zhiqiang Liu ◽  
Huaping Gui ◽  
Lizhao Geng ◽  
Juan Wei ◽  
...  
Keyword(s):  
Protein Binding ◽  
Genome Editing ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Selection System ◽  
Transcription Activator ◽  
Efficient Generation ◽  
A Genome ◽  
Transgenic Pollen ◽  
Selection Of

Abstract Background Rice leaf blight, which is a devastating disease worldwide, is caused by the bacterium Xanthomonas oryzae pv. oryzae (Xoo). The upregulated by transcription activator-like 1 (UPT) effector box in the promoter region of the rice Xa13 gene plays a key role in Xoo pathogenicity. Mutation of a key bacterial protein-binding site in the UPT box of Xa13 to abolish PXO99-induced Xa13 expression is a way to improve rice resistance to bacteria. Highly efficient generation and selection of transgene-free edited plants are helpful to shorten and simplify the gene editing-based breeding process. Selective elimination of transgenic pollen of T0 plants can enrich the proportion of T1 transgene-free offspring, and expression of a color marker gene in seeds makes the selection of T2 plants very convenient and efficient. In this study, a genome editing and multiplexed selection system was used to generate bacterial leaf blight-resistant and transgene-free rice plants. Results We introduced site-specific mutations into the UPT box using CRISPR/Cas12a technology to hamper with transcription-activator-like effector (TAL) protein binding and gene activation and generated genome-edited rice with improved bacterial blight resistance. Transgenic pollen of T0 plants was eliminated by pollen-specific expression of the α-amylase gene Zmaa1, and the proportion of transgene-free plants increased from 25 to 50% among single T-DNA insertion events in the T1 generation. Transgenic seeds were visually identified and discarded by specific aleuronic expression of DsRed, which reduced the cost by 50% and led to up to 98.64% accuracy for the selection of transgene-free edited plants. Conclusion We demonstrated that core nucleotide deletion in the UPT box of the Xa13 promoter conferred resistance to rice blight, and selection of transgene-free plants was boosted by introducing multiplexed selection. The combination of genome editing and transgene-free selection is an efficient strategy to accelerate functional genomic research and plant breeding.

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