scholarly journals Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
TENGKU SITI HABSYAH ◽  
Mohammad Basyuni ◽  
LUTHFI A.M. SIREGAR ◽  
DADANG AFANDI ◽  
INDRA SYAHPUTRA
Keyword(s):  
Total Lipid ◽  
Lipase Activity ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Leaf Tissue ◽  
Dry Weight ◽  
Carbon Chain ◽  
Chain Lengths ◽  
North Sumatra ◽  
Palm Leaf

Abstract. Habsyah TS, Basyuni M, Siregar LAM, Afandi D, Syahputra I. 2021. Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity. Biodiversitas 22: 830-837. The aim of this study was to determine the pattern of chemical compounds from the polyisoprenoid group in oil palm plants that were contributing to the lipase activity. The study was conducted in two locations. A sampling of leaves and fruit mesocarp from 90 progenies was conducted at PT. Socfin Indonesia Aek Loba Plantation, Asahan District, North Sumatra, Indonesia. Biochemical analysis of polyisoprenoid was carried out at the Forest Biotechnology Laboratory, Faculty of Forestry, University of Sumatera Utara, Medan. The results showed that the total lipids in leaves tissue ranged from 0.40 to 1.032 mg/g, with the lowest total lipid found in SL-4966 progeny and the highest total lipid found in SL-9737 progeny. Polyisoprenoid values ​​ranged from 1.63 to 10.82 mg/g dry weight. The highest polyisoprenoid content was obtained in SL-10682 progeny, and the lowest was in SL-5045 progeny leaf tissue. The polyisoprenoid ​​in fruit mesocarp tissue ranged from 1.79 to 4.14 mg/g. The lowest polyisoprenoid content (1.79 mg/g) of fruit mesocarp was found in SL-4233 progeny, while the highest (4.14 mg/g) was found in SL-5010 progeny.  Dolichol and polyprenol are equally dominant in palm leaves.  Carbon polyprenol chain lengths ranged from C45-C105, and dolichol ranged from C50-C110. The polyprenol carbon chain lengths in the mesocarp tissues were C65-C120 and dolichol was C65-C125. Polyisoprenoid analysis on oil palm leaf and fruit mesocarp tissue showed that lipid patterns of oil palm leaf and mesocarp were categorized as type II lipid patterns. It provides important information for selecting genotypes of low lipase activity of oil palm plants.  

scholarly journals Expression of oil palm (Elaeis guineensis) polyisoprenoids in response to Ganoderma boninense infection

2018 ◽  
Vol 20 (1) ◽  
pp. 68-76
Author(s):  
DADANG AFANDI ◽  
MOHAMMAD BASYUNI ◽  
LOLLIE AGUSTINA P. PUTRI ◽  
DIANA CHALIL ◽  
INDRA SYAHPUTRA
Keyword(s):  
Chain Length ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Leaf Tissue ◽  
Carbon Chain ◽  
Root Tissue ◽  
Carbon Chain Length ◽  
Ganoderma Boninense ◽  
Root Diseases ◽  
A Chain

Afandi D, Basyuni M, Putri LAP, Chalil D, Syahputra I. 2019. Expression of oil palm (Elaeis guineensis) polyisoprenoids in response to Ganoderma boninense infection. Biodiversitas 20: 68-76. Currently, oil palm is an important economic crop and has become one of the world's major vegetable oils as well as a potential source of biodiesel. Unfortunately, oil palm plantations in Asia, particularly in Indonesia and Malaysia face the threat of basal stem root diseases caused by Ganoderma sp. Various methods and approaches have been made to select the oil palm that is tolerant to Ganoderma boninense, among others using biochemical selection. This research aimed to analyze polyisoprenoids expressionof oil palm tolerant to G. boninense using two-dimensional thin layer chromatography (2D-TLC). The plant material used in this trial weretwo cross-series of genetic materials belonging to PT Socfindo, that were known to have certain levelsof tolerance to G. boninense. The first wasa cross-series of 15-year-old oil palm in the field, and the secondwasa new cross-series for an early detection in the nursery stage. The results showed that there werediversitiesin the expression of polyisoprenoids between tissues, treatments, and level of tolerance. Polyprenols with a chain length of C45-C65 and dolichols of C45-C55were detected in the leaf tissue but not found in the root tissue. Polyprenols with a carbon chain length of C80-C100 occurred in infected palm root tissue but did not in the healthy oil palm. The increase of polyisoprenoid (polyprenol and dolichol) in infected and inoculated root tissues it is presumablydue to the plant biochemical response to the presence of G. boninense attack. Cluster analysis demonstrated distinct groups of polyisoprenoid carbon-chains betweenroot and leaf tissues of oil palm mature and seedling. Interestingly, in the absence of G. boninense infection, the polyisoprenoid carbon chain pattern in the tolerant oil palm seedlingroot tissue is different from that in the susceptible seedlings. Thus, the polyisoprenoid carbon chain pattern can be considered as a potential biochemical marker for the screening of oil palm tolerance to G. boninense.

scholarly journals Respons Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Pemberian Pupuk Organik Asal Pelepah Kelapa Sawit dan Pupuk Majemuk NPK

Agrikultura ◽  
2017 ◽  
Vol 28 (2) ◽  
Author(s):  
Mira Ariyanti ◽  
Gita Natali ◽  
Cucu Suherman
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Block Design ◽  
Organic Fertilizer ◽  
Dry Weight ◽  
Organic Fertilizers ◽  
Compound Fertilizer ◽  
Elaeis Guineensis Jacq ◽  
Four Levels ◽  
Palm Fronds

ABSTRACTThe growth response of oil palm (Elaeis Guineensis Jacq.) seedling toward the application of organic fertilizer from palm fronds and NPK compound fertilizerThe research was aimed to study the influence between organic fertilizers from palm fronds and NPK compound fertilizer to reduce NPK compound fertilizer in main nursery. The experiment was conducted from January to April 2017 at the Experiment Station Ciparanje, Faculty of Agriculture, Universitas Padjadjaran. The experimental design used was Randomized Block Design that arranged in factorial patterns with two factors and three replications. The fisrt factor was dosage of organic fertilizers from palm fronds consisted of three levels of 0 g/polybag, 800 g/polybag, and 1600 g/polybag and the second factor was dosage of NPK compound fertilizer consisted of four levels of 0 g/polybag, 20 g/polybag, 40 g/polybag, and 60 g/polybag. The result of the experiment showed that there was interaction effect between organic fertilizers from palm fronds and NPK compound fertilizer on height of seedling and dry weight of the shoot. The dosage of 1600 g/polybag organic fertilizers from palm fronds with the dosage of 20 g/polybag NPK compound fertilizer showed the best result in dry weight of the shoot.Keywords: Oil palm seedling, Main nursery, Organic fertilizer, Palm frond, NPK compound fertilizerABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh interaksi antara pupuk organik asal pelepah kelapa sawit dengan pupuk majemuk NPK yang baik untuk mengurangi penggunaan pupuk majemuk NPK di pembibitan utama kelapa sawit. Percobaan dilaksanakan dari bulan Januari sampai dengan April 2017 di Kebun Percobaan Ciparanje, Fakultas , Universitas Padjadjaran. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok dengan pola faktorial yang diulang sebanyak tiga kali. Faktor pertama meliputi dosis pupuk organik asal pelepah kelapa sawit terdiri dari tiga taraf yaitu 0 g/polybag, 800 g/polybag, dan 1600 g/polybag dan faktor kedua dosis pupuk majemuk NPK yang terdiri empat taraf yaitu 0 g/polybag, 20 g/polybag, 40 g/polybag, dan 60 g/polybag. Hasil percobaan menunjukkan bahwa terdapat pengaruh interaksi pupuk organik asal pelepah kelapa sawit dengan pupuk majemuk NPK terhadap tinggi tanaman dan bobot kering tajuk. Perlakuan pupuk organik asal pelepah kelapa sawit 1600 g/bibit dengan pupuk majemuk NPK 20 g/bibit menghasilkan bobot kering tajuk bibit kelapa sawit terbaik.Kata Kunci: Bibit kelapa sawit, Pembibitan utama, Pupuk organik, Pelepah kelapa sawit, NPK

scholarly journals Factors Affecting Productivity of Oil Palm (Elaeis guineensis Jacq.) at Various Afdelings in Bah Jambi Farm PT. Perkebunan Nusantara IV

2022 ◽  
Vol 9 (1) ◽  
pp. 99-110
Author(s):  
Amir Fhad Sastranegara Harahap ◽  
Mochammad Munir
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Light Exposure ◽  
Descriptive Analysis ◽  
Secondary Data ◽  
Exchange Capacity ◽  
Coefficient Of Determination ◽  
Organic C ◽  
Factors Affecting ◽  
North Sumatra

North Sumatra Province, where the second-highest oil palm productivity in Indonesia, has successfully reached fresh fruit bunches (FFB) production of 5,775,631.82 tons in 2016. However, the level of oil palm productivity tends to be unstable and low. The purpose of this study was to identify and analyze factors influencing the level of oil palm productivity at the Bah Jambi Plantation PTPN IV, Simalungun Regency, North Sumatra Province. The method used in this study was the qualitative descriptive analysis method by collecting secondary data at research locations at 4 Afdeling Kebun Bah Jambi PT. Nusantara IV Plantation. The results of correlation and regression analysis showed that soil factor such as organic C, soil pH, cation exchange capacity and the availability of soil N, P, K and Mg is the most dominant factors in influencing the amount of oil palm productivity which have determination coefficient (R2) more than 90%. Meanwhile, climate factors such as evapotranspiration, duration of light exposure, wind speed and rainfall have the most role in influencing oil palm productivity which has a coefficient of determination (R2) of 95%, 94%, 88% and 33%.

scholarly journals Cluster analysis of polyisoprenoid in oil palm (Elaeis guineensis) leaves in different land-uses to find the possible cause of yield gap from planting materials

2018 ◽  
Vol 19 (4) ◽  
pp. 1492-1501 ◽  
Author(s):  
MOHAMMAD BASYUNI ◽  
RIDHA WATI ◽  
IRMA DENI ◽  
ANANDA RATU TIA ◽  
ETTI SARTINA SIREGAR ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Oil Palm ◽  
Paddy Field ◽  
Elaeis Guineensis ◽  
Type I ◽  
Land Uses ◽  
Yield Gap ◽  
Planting Materials ◽  
North Sumatra ◽  
Possible Cause

 Basyuni M, Wati R, Deni I, Tia AR, Slamet B, Siregar ES, Syahputra I. 2018. Cluster analysis of polyisoprenoid in oil palm(Elaeis guineensis) leaves in different land-uses to find the possible cause of yield gap from planting materials. Biodiversitas 19: 1492-1501. The distribution and occurrence of polyprenols and dolichols in the leaves of oil palm (Elaeis guineensis Jacq.) plantations indifferent land-uses in North Sumatra, Indonesia were analyzed using two-dimensional thin layer chromatography (2D-TLC). Eighty-oneof oil palm leaves were sampled to represent twenty-seven sites of land-uses, namely paddy field (four locations), mangrove (threelocations) in Lubuk Kertang, Langkat, North Sumatra. In addition, samples from four groups of smallholders in Stabat, Langkat, sixsites in Bangun Bandar, Serdang Bedagai, and ten sites on the campus of Universitas Sumatera Utara (USU), North Sumatra werecollected. In the leaves, only one type (type II) with respect to the distribution of polyisoprenoids was detected: having the presence ofboth polyprenols and dolichols. Either type I, having predominance of dolichols over polyprenols or type III, displaying dominatingpolyprenols over dolichols were not observed. Results also showed that chain-length distribution of ficaprenols (C50-C60) without longerpolyprenols (C85-C100) and dolichols of C85-C100 was detected in the paddy field, mangroves, and one site in USU campus. Thispolyisoprenoid profile was close to dura type of Elaeis guineensis. By contrast, the remaining land-uses had ficaprenols and longerpolyprenols, and dolichols (C85-C100), which belong to tenera or pisifera type. To confirm this finding, a dendrogram was constructed.Cluster analysis demonstrated that twenty-seven sites of E. guineensis were grouped into appropriate types of dura, pifiera, and teneraaccordingly, indicating that the existence of polyisoprenoids in E. guineensis was a chemotaxonomic marker. The finding ofpolyisoprenoid pattern of E. guineensis as dura type in mangrove and paddy field sites may reveal significant causes of yield gap in oilpalm plantation from planting materials.

scholarly journals GROWTH PROFILE ANALYSIS OF OIL PALM BY USING SPOT 6 THE CASE OF NORTH SUMATRA

2017 ◽  
Vol 12 (1) ◽  
pp. 21
Author(s):  
Ita Carolita ◽  
J. Sitorus ◽  
Johannes Manalu ◽  
Dhimas Wiratmoko
Keyword(s):  
Regression Analysis ◽  
High Resolution ◽  
Satellite Data ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Profile Analysis ◽  
Secondary Data ◽  
Profile Data ◽  
Growth Profile ◽  
North Sumatra

Oil Palm (Elaeis guineensis Jack.) is one of the world’s most important tropical tree crops. Its expansion has been reported to cause widespread environment impacts. SPOT 6 data is one of high resolution satellite data that can give information more detail about vegetation and the age of oil palm plantation. The objective of this study was to analyze the growth profile of oil palm and to estimate the productivity age of oil palm. The study area is PTP N 3 in Tebing Tinggi North Sumatera Indonesia.  The method that used is NDVI analysis and regression analysis for getting the model of oil palm growth profile. Data from the field were collected as the secondary data to build that model. The data that collected were age of oil palm and diameters of canopy for every age.   Results indicate that oil palm growth can be explained by variation of NDVI with formula y = -0.0004x2 + 0.0107x + 0.3912, where x is oil palm age and  Y is NDVI of SPOT, with R² = 0.657. This equation can be used to predict the age of oil palm for range 4 to 11 years with R2 around 0.89.

scholarly journals Screening of wild oil palm (Elaeis guineensis) germplasm for lipase activity – CORRIGENDUM

2016 ◽  
Vol 154 (7) ◽  
pp. 1253-1253
Author(s):  
Y. T. WONG ◽  
A. KUSHAIRI ◽  
N. RAJANAIDU ◽  
M. OSMAN ◽  
R. WICKNESWARI ◽  
...  
Keyword(s):  
Lipase Activity ◽  
Oil Palm ◽  
Elaeis Guineensis

scholarly journals Phenazine from Pseudomonas aeruginosa UPMP3 induced the host resistance in oil palm (Elaeis guineensis Jacq.)-Ganoderma boninense pathosystem

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Waheeda Parvin ◽  
Nisha Govender ◽  
Radziah Othman ◽  
Hawa Jaafar ◽  
Mahbubur Rahman ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Oil Palm ◽  
Biocontrol Agent ◽  
Elaeis Guineensis ◽  
Block Design ◽  
Dry Weight ◽  
Post Inoculation ◽  
Fresh Weight ◽  
Plant Vigour ◽  
Basal Stem Rot

Abstract Pseudomonas aeruginosa developed its biocontrol agent property through the production of antifungal derivatives, with the phenazine among them. In this study, the applications of crude phenazine synthesized by Pseudomonas aeruginosa UPMP3 and hexaconazole were comparatively evaluated for their effectiveness to suppress basal stem rot infection in artificially G. boninense-challenged oil palm seedlings. A glasshouse experiment under the randomized completely block design was set with the following treatments: non-inoculated seedlings, G. boninense inoculated seedlings, G. boninense inoculated seedlings with 1 mg/ml phenazine application, G. boninense inoculated seedlings with 2 mg/ml phenazine application and G. boninense inoculated seedlings with 0.048 mg/ml hexaconazole application. Seedlings were screened for disease parameters and plant vigour traits (plant height, plant fresh weight, root fresh, and dry weight, stem diameter, and total chlorophyll) at 1-to-4 month post-inoculation (mpi). The application of 2 mg/ml phenazine significantly reduced disease severity (DS) at 44% in comparison to fungicide application (DS = 67%). Plant vigour improved from 1 to 4 mpi and the rate of disease reduction in seedlings with phenazine application (2 mg/ml) was twofold greater than hexaconazole. At 4, 6 and 8 wpi, an up-regulation of chitinase and β-1,3 glucanase genes in seedlings treated with phenazine suggests the involvement of induced resistance in G. boninense-oil palm pathosystem.

scholarly journals Pemanfaatan beberapa jenis urin ternak sebagai pupuk organik cair dengan konsentrasi yang berbeda pada tanaman kelapa sawit (Elaeis guineensis jacq.) di pembibitan utama

Kultivasi ◽  
2018 ◽  
Vol 17 (2) ◽  
Author(s):  
Bisri Alvi ◽  
Mira Ariyanti ◽  
Yudithia Maxiselly
Keyword(s):  
Oil Palm ◽  
Seed Quality ◽  
Elaeis Guineensis ◽  
Organic Liquid ◽  
Block Design ◽  
Organic Fertilizer ◽  
Dry Weight ◽  
Fertilizer Application ◽  
Good Effect ◽  
Liquid Fertilizer

Sari. Pembibitan merupakan tahapan awal dalam budidaya tanaman kelapa sawit, kualitas bibit akan mempengaruhi hasil yang akan diperoleh nantinya. Faktor yang mempengaruhi pertumbuhan bibit diantaranya adalah ketersediaan unsur hara yang dapat diperoleh dari pemberian pupuk anorganik dan organik. Pemberian pupuk anorganik tanpa diimbangi pupuk organik dapat merusak sifat tanah, sehingga diperlukan pupuk organik yaitu dengan memanfaatkan urin ternak sebagai pupuk organik cair, dengan menambahkan pupuk organik cair pada tanah, maka dapat membantu proses pertumbuhan tanaman karena pupuk organik cair urin ternak mengandung hormon pertumbuhan bagi tanaman serta mudah diserap tanaman. Penelitian dilakukan di Kebun Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran, Kabupaten Sumedang, pada bulan September 2017 sampai bulan Februari 2018. Ordo tanah yang digunakan adalah Inceptisol. Tipe curah hujan menurut klasifikasi Schmidt dan Ferguson bertipe C dengan ketinggian tempat ±780 m dpl. Penelitian menggunakan rancangan acak kelompok (RAK) dengan 11 perlakuan yang diulang sebanyak 3 kali dengan jumlah tanaman di setiap plot 2 tanaman. Perlakuan terdiri dari pemberian urin sapi, kambing dan kelinci dengan konsentrasi 40 mL/L air, 120 mL/L air dan 200 mL/L air, serta perlakuan kontrol (tanpa perlakuan) dan pemberian pupuk urea 3,3 g/tanaman. Hasil penelitian menunjukkan bahwa pemanfaatan urin ternak memberikan pengaruh baik terhadap pertumbuhan tanaman, yang tercermin dari bobot kering tanaman. Perlakuan urin kambing konsentrasi 40 mL/L air dan 120 mL/L air cenderung berpengaruh baik terhadap bobot kering tajuk, bobot kering akar, dan nisbah tajuk akar bibit kelapa sawit.Kata Kunci: urin ternak, urin sapi, urin kambing, urin kelinci, kelapa sawit. Abstract. Seedling is an initial stage in the cultivation of oil palm, seed quality will affect the results that will be obtained later. Factor affecting the growth of seedlings of which the availability of nutrients which can be obtained from inorganic and organic fertilizer. Application of inorganic fertilizer without an organic fertilizer balanced can be damage the nature of the soil, necessitating organic fertilizer the urine of livestocks as organic liquid, adding organic liquid fertilizer to the soil, can help the plants to growth because organic liquid fertilizer of livestocks urine contain growth hormone for plants and easily absorbed to the plants. The research was conducted in Experimental Station of Ciparanje, Faculty of Agriculture, Padjadjaran University, Sumedang, from September 2017 to February 2018. Ordo of the soil used is Inceptisol. Precipitation type according to Schmidt and Ferguson's classification of type C with ± 780 meters above sea level altitude. Experiment was using a randomized block design (RBD) with 11 treatments with 3 replications and the number of plants in each plot of 2 plants. The treatment consists the urine of cows, goats and rabbits with some concentration of 40 mL/L of water, 120 mL/L of water  and 200 mL/L of water as well as a comparison treatment, control (untreated) and the provision of urea fertilizer 3,3 g/plant. The results showed that the utilization some kinds of cattle urine provides a good effect on plant growth, which is reflected from the dry weight of the plant. Treatment goat’s urine concentration 40 mL/L of water and 120 mL/L of water tends to affect on the dry weight shoot, dry weight root, and shoot root ratio on seedling oil palm.Keywords: livestock urine, cow’s urine, goat’s urine, rabbit’s urine, oil palm.

scholarly journals Glyphosate resistance of Eleusine indica populations from North Sumatra, Indonesia

2019 ◽  
Vol 20 (7) ◽  
Author(s):  
KOKO TAMPUBOLON ◽  
EDISON PURBA ◽  
MOHAMMAD BASYUNI ◽  
DIANA SOFIA HANAFIAH
Keyword(s):  
Oil Palm ◽  
Block Design ◽  
Dry Weight ◽  
Glyphosate Resistance ◽  
Resistance Level ◽  
Eleusine Indica ◽  
Survival Percentage ◽  
Randomized Block Design ◽  
Similar Mode ◽  
North Sumatra

Abstract. Tampubolon K, Purba E, Basyuni M, Hanafiah DS. 2019. Glyphosate resistance of Eleusine indica populations from North Sumatra, Indonesia. Biodiversitas 20: 1910-1916.  Controlling of Eleusine indica populations in oil palm plantations using a similar mode of action of herbicide may cause resistant weed. Overall E. indica populations from oil palm plantations in North Sumatra, Indonesia have never been reported to be glyphosate-resistant. This research was aimed to determine the survival percentage and to classify the resistance of E. indica to glyphosate from oil palm plantations in 11 districts in North Sumatra Province. The research was conducted in the Weed Research Center Field Laboratory, Faculty of Agriculture, Universitas Sumatera Utara from October 2016 to August 2017. This research was arranged in a Randomized Block Design with the glyphosate-treatment using a recommended dose of two l ha-1 and three replications. The ESU0 population was collected in the soccer field of Politeknik Negeri Medan (Medan city) was used as a check, and has never been exposed to the herbicide. Parameters were analyzed using one-way ANOVA, and the means were compared using DMRT at a probability level of 5%. The results showed that 276 (65.56%) of 421 populations were classified as glyphosate-resistant, 88 populations (20.90%) were classified as glyphosate-resistant developing, and 57 populations (13.54%) were classified as glyphosate-susceptible. The highest range of dry weight of glyphosate-resistant E. indica populations were found in 5 districts including South Labuhanbatu District followed by the Serdang Bedagai, Simalungun, Asahan, and North Labuhanbatu Districts. The observed resistance level and dry weight range in the present study indicated the failure in controlling E. indica due to continued use of glyphosate and the potency of E. indica distribution into other oil palm estates due to its high biomass production capability.

Sign in / Sign up

Export Citation Format

Share Document