scholarly journals Larval anisakids parasitizing the blue whiting, Micromesistius poutassou, from Motril Bay in the Mediterranean region of southern Spain

2000 ◽  
Vol 74 (4) ◽  
pp. 361-364 ◽  
Author(s):  
A. Valero ◽  
J. Martín-Sánchez ◽  
E. Reyes-Muelas ◽  
F.J. Adroher

AbstractA total of 301 blue whiting, Micromesistius poutassou Risso, 1826, ranging in length from 17 to 28 cm, from Motril Bay (Mediterranean coast, south Spain) were examined for anisakid nematodes, as these fish are common items in the Spanish Mediterranean diet. Three anisakid species were morphologically identified with a total prevalence of 10.63%. Anisakis simplex s.l. Rudolphi, 1809 had a prevalence value of 6.65%, compared with 2.66% for A. physeteris Baylis, 1923 and 2.33% for Hysterothylacium aduncum Rudolphi, 1802. Variations in prevalence values with season and host size are discussed. Allozyme markers (leucine aminopeptidase-1) were used to identify anisakid nematodes assigned to the A. simplex complex and all examined larvae were found to correspond genetically to A. pegreffii Nascetti et al., 1986.

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 862
Author(s):  
Ana Elena Ahuir-Baraja ◽  
Lola Llobat ◽  
Maria Magdalena Garijo

Anisakidosis is a parasitic zoonotic disease which can cause gastroallergic reactions in humans. In 2010, the European Food Safety Agency estimated that approximately 20,000 cases of anisakiasis had been reported across the world, with Spain having the highest number of infections in Europe. The blue whiting (Micromesistius poutassou, Risso, 1827) is one of the most widely fished species worldwide and represents around 25% of the white fish eaten in Spain. The Spanish Food Safety Authority requires obligatory evisceration of certain fish species before commercialization, but not for blue whiting. Nevertheless, some supermarkets carry this out themselves to prevent human infections and negative customer reactions deriving from the presence of ascaridoid larvae. To assess the effectiveness of eviscerations at supermarkets, a total of 320 blue whiting specimens were examined. The risk of larval migration from the visceral cavity to the musculature in gutted and ungutted fish was also assessed. Our results showed a total prevalence (25%) of ascaridoids in fish gutted at the supermarket, and a direct relationship was found between the presence of larvae in the muscle and time until evisceration. In ungutted fish, the standard length and weight were higher for infected than for non-infected fish. Also, massive infections had a higher prevalence in these larger specimens, in which the viability of larvae was also high. Larval viability was not found to be affected by a 24-h refrigeration period. Anisakis was the most prevalent genus identified in the fish examined. The results indicate that gutting at the supermarket is not an effective method for the total removal of ascaridoid larvae and that additional safety measures are advisable.


Genes ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 321 ◽  
Author(s):  
Serena Cavallero ◽  
Fabrizio Lombardo ◽  
Marco Salvemini ◽  
Antonella Pizzarelli ◽  
Cinzia Cantacessi ◽  
...  

Ascaridoid nematodes are widespread in marine fishes. Despite their major socioeconomic importance, mechanisms associated to the fish-borne zoonotic disease anisakiasis are still obscure. RNA-Seq and de-novo assembly were herein applied to RNA extracted from larvae and dissected pharynx of Hysterothylacium aduncum (HA), a non-pathogenic nematode. Assembled transcripts in HA were annotated and compared to the transcriptomes of the zoonotic species Anisakis simplex sensu stricto (AS) and Anisakis pegreffii (AP). Approximately 60,000,000 single-end reads were generated for HA, AS and AP. Transcripts in HA encoded for 30,254 putative peptides while AS and AP encoded for 20,574 and 20,840 putative peptides, respectively. Differential gene expression analyses yielded 471, 612 and 526 transcripts up regulated in the pharynx of HA, AS and AP. The transcriptomes of larvae and pharynx of HA were enriched in transcripts encoding collagen, peptidases, ribosomal proteins and in heat-shock motifs. Transcripts encoding proteolytic enzymes, anesthetics, inhibitors of primary hemostasis and virulence factors, anticoagulants and immunomodulatory peptides were up-regulated in AS and AP pharynx. This study represents the first transcriptomic characterization of a marine parasitic nematode commonly recovered in fish and probably of negligible concern for public health.


2018 ◽  
Vol 6 (1) ◽  
pp. 77-82
Author(s):  
Fahdi Putra Utama ◽  
Kismiyati . ◽  
Gunanti Mahasri ◽  
Putri Desi Wulansari

ABSTRACT                The High Interest of public to Marine fish, create more demand of Slander Scad (Decapterus macrosoma). Slander Scad is one commodity that has economic value and high nutritional content. The Slander Scad is obtained from the catch of fishermen in the Nusantara Fishery Port Brondong, Lamongan. Fish that caught from the wild is likely to get a disease caused by infection of the endoparasites.worm.           The parasites that infect Slander scad probably caused by environmental factors that could decrease immune system, causing the fish easily infected by endoparasites worm such as Anisakis. This parasite is zoonotic and can infect humans, therefore, to identify and prevalence for humans who consume fish can manage properly. The purpose of this study was to identify the species of endoparasite worms that infect Slander Scad (D. Macrosoma) in Nusantara Fishery Port of Brondong, Lamongan. The research method is using a survey method through sampling at locations directly. 75 samples of Slander Scad were examined. The main parameters observed in this study are to observe the species and the prevalence of endoparasitic worm that infected Slander scad. The result of the identification of endoparasite that infected Slander Scad were analyzed descriptively and presented in the form of figures and tables. The results showed the presence of the third-stage Anisakis simplex larvae that infect the Slander Scad in the liver, intestines, gonads, muscles in the abdominal side, in the Nusantara Fishery Port of Brondong Lamongan, East Java with a total prevalence of Anisakis simplex worm was 42.67%.                               Keywords: Slander Scad, Prevalence, Anisakis simplex, and zoonotic.


Author(s):  
D. P. Tsygankova ◽  
K. Е. Krivoshapova ◽  
O. L. Barbarash

Mediterranean diet is one of the most effective in prevention of cardiovascular pathology. It has been approved evidently in the countries of the Mediterranean region, and demands for efficacy evaluation in populations of other climatic and geographic circumstances, with specific ethnic and religious traditions. In this article, the main studies are explored, in the investigation of Mediterranean diet in countries of non-Mediterranean areas. The problems discussed, of the inhabitants of these countries that interfere with high adherence to such model of nutrition.


2020 ◽  
pp. e1781
Author(s):  
Jenniffer Alejandra Castellanos Garzon ◽  
Rubén Mercado P ◽  
Sebastián Peña F ◽  
María Carolina Pustovrh R ◽  
Liliana Salazar M

Objetivo. Identificar nematodos de la familia Anisakidae en el pez de consumo Mugil curema. Materiales y métodos. Para este estudio, se recolectaron 16 peces Lisa (M. curema) del puerto de Tumaco, una ciudad en la costa colombiana del Pacífico. La identificación morfológica de las larvas se realizó mediante taxonomía clásica y se calculó el porcentaje de infestación de larvas. Para la identificación molecular, se realizó una PCR múltiple con cebadores para las especies Anisakis physeteris, Pseudoterranova decipiens, Anisakis simplex sensu stricto, Contracaecum osculatum, Hysterothylacium aduncum y Anisakis pegreffii. Resultados. La revisión taxonómica permitió la identificación de larvas de tipo II del género Anisakis y larvas del género Pseudoterranova. Las larvas se aislaron principalmente del intestino, donde se encontró que el 94% de los peces estaban parasitados por nematodos anisakidos. La PCR multiplex permitió la identificación de la especie A. physeteris (Larva tipo II) y P. decipiens. Conclusiones. Este estudio en el primer reporte del nematodo Anisakidae en Tumaco, Colombia. Estos resultados proporcionan una justificación convincente para un estudio adicional sobre la familia Anisakidae en Colombia, como un problema de salud pública.


2019 ◽  
Vol 121 (3) ◽  
pp. 725-737 ◽  
Author(s):  
Mahmut Genc ◽  
Seda Genc

Purpose The purpose of this paper is to evaluate the adherence and abandonment of the Turkish emerging adults in an Izmir University located at western Mediterranean coast of Turkey and to assess potential associations with anthropometric characteristics. Design/methodology/approach A cross-sectional survey (n=494, 18–27 years) carried out in 2017 among emerging adults in University. KIDMED Index was used to assess the degree of adherence Mediterranean Diet (MedDiet). The associations of KIDMED scores with demographic, residency and anthropometric were separately reported. The data were collected through standardized questionnaires directly from participants. Findings The average KIDMED score was calculated 4.86±2.5. Optimum adherence to the MedDiet was found only in 13.0 percent of participants, whereas 32.6 percent had poor adherence levels. Considering self-reported anthropometric data, the BMI values of the population was calculated as 22.3±3.9 kg/m2. In population, 13.9 percent of the subjects were underweight, while 16.0 percent were overweight and 3.9 percent obese. A significant association was found between BMI and KIDMED scores both in genders and residency. Originality/value This is the first study reporting the level of adherence to the MedDiet among Turkish emerging adults in terms of residency during education and the first KIDMED study conducted in Izmir located at Aegean Sea. The results support previously proposed transition concept by several scholar from different Mediterranean countries: it was found that only 13 percent of young adults having desired dietary habits in an Aegean city with local traditional cuisine highly affected by Cretan cuisine. These results are significant for University managements and health authorities in order to take actions for returning this transition contrariwise beginning with these groups.


2000 ◽  
Vol 74 (2) ◽  
pp. 151-154 ◽  
Author(s):  
S.M. Laffon-Leal ◽  
V.M. Vidal-Martínez ◽  
G. Arjona-Torres

AbstractFive fish species used for preparation of a popular dish (cebiche) made with raw fish flesh in Mexico were obtained from five localities of the coast of Yucatan. Lutjanus synagris, Gerres cinereus, Sphyraena barracuda, Epinephelus morio and Haemulon plumieriwere examined for the presence of larvae of anisakid nematodes, causative agents of human anisakiasis. The nematode Pseudoterranova sp. was found in E. morio and S. barracuda with a total prevalence of 83% and 6.5 ± 6.2 worms per fish for E. morio, and a prevalence of 33% and 10.2 ± 30.0 worms per fish forS. barracuda. Contracaecumsp. was found to infect G.cinereus with a prevalence of 57% and 7.6 ± 11.4 worms per fish. The relatively high prevalence of Pseudoterranova sp. indicates that this parasite is a potential causal agent of anisakiasis on the coast of Yucatan. Although all larvae were found only in the mesentery of the fish host, their importance as a potential source of human infection cannot be excluded as larval migration to the muscles in dead fish is possible.


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