Dracunculus insignis: experimental infection in the ferret,Mustela putorius furo

1988 ◽  
Vol 62 (3) ◽  
pp. 265-270 ◽  
Author(s):  
M. L. Eberhard ◽  
E. Ruiz-Tiben ◽  
S. V. Wallace
Keyword(s):  
Animal Model ◽  
Laboratory Study ◽  
Experimental Infection ◽  
Laboratory Animal ◽  
Gravid Female ◽  
Mustela Putorius ◽  
Infective Larvae ◽  
The North ◽  
Gravid Females ◽  
Mustela Putorius Furo

ABSTRACTThe laboratory study of dracunculiasis has suffered from the lack of a suitable, readily available animal model. We have been able to experimentally infect ferrets,Mustela putorius furo, with the North American dracunculid,Dracunculus insignis. Ferrets were inoculated with 75 to 100 infective larvae and were necropsied 90 to 240 days later. Guinea worms were recovered from 10 (56%) of 18 ferrets. A total of 44 worms were recovered, for an average of 4.4 worms per infected ferret. Gravid female worms were recovered as early as 128 days postinoculation. Thirteen (87%) of 15 gravid female worms were recovered from the extremities. Living male worms were recovered at 200 days of age, indicating that not all male worms die shortly after mating. First-stage larvae recovered from gravid females as early as 200 days of age were found to be infective to the copepod.Acanthocyclops vernalis. These observations suggest that the ferret is an excellent laboratory animal for dracunculiasis research.

scholarly journals Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Greg A. Kirchenbaum ◽  
Ted M. Ross
Keyword(s):  
Animal Model ◽  
Monoclonal Antibodies ◽  
B Cells ◽  
B Cell ◽  
Mononuclear Cells ◽  
Unmet Need ◽  
Mustela Putorius ◽  
Peripheral Blood Mononuclear ◽  
Antibody Secreting Cells ◽  
Mustela Putorius Furo

The domestic ferret (Mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease, most notably influenza. Despite the importance of this animal model, characterization of the immune response by flow cytometry (FCM) is severely hampered due to the limited number of commercially available reagents. To begin to address this unmet need and to facilitate more in-depth study of ferret B cells including the identification of antibody-secreting cells, eight unique murine monoclonal antibodies (mAb) with specificity for ferret immunoglobulin (Ig) were generated using conventional B cell hybridoma technology. These mAb were screened for reactivity against ferret peripheral blood mononuclear cells by FCM and demonstrate specificity for CD79β+B cells. Several of these mAb are specific for the light chain of surface B cell receptor (BCR) and enable segregation of kappa and lambda B cells. Additionally, a mAb that yielded surface staining of nearly all surface BCR positive cells (i.e., pan ferret Ig) was generated. Collectively, these MαF-Ig mAb offer advancement compared to the existing portfolio of polyclonal anti-ferret Ig detection reagents and should be applicable to a wide array of immunologic assays including the identification of antibody-secreting cells by FCM.

scholarly journals SARS-CoV-2 Seroprevalence in Household Domestic Ferrets (Mustela putorius furo)

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 667
Author(s):  
Jacobo Giner ◽  
Sergio Villanueva-Saz ◽  
Ana Pilar Tobajas ◽  
María Dolores Pérez ◽  
Ana González ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Animal Species ◽  
Enzyme Linked Immunosorbent Assay ◽  
Receptor Binding Domain ◽  
Mustela Putorius ◽  
Natural Conditions ◽  
Serum Samples ◽  
Source Of Infection ◽  
First Time ◽  
Mustela Putorius Furo

Animal infections with SARS-CoV-2 have been reported in different countries and several animal species have been proven to be susceptible to infection with SARS-CoV-2 both naturally and by experimental infection. Moreover, infections under natural conditions in more than 20 mink farms have been reported where humans could have been the source of infection for minks. However, little information is available about the susceptibility of pet animals under natural conditions and currently there is no SARS-CoV-2 epidemiological assessment occurrence in household ferrets. In this study, the presence of SARS-CoV-2 antibodies was evaluated in serum samples obtained from 127 household ferrets (Mustela putorius furo) in the Province of Valencia (Spain). Two ferrets tested positive to SARS-CoV-2 (1.57%) by in-house enzyme-linked immunosorbent assay based on receptor binding domain (RBD) of Spike antigen. Furthermore, anti-RBD SARS-CoV-2 antibodies persisted at detectable levels in a seropositive SARS-CoV-2 domestic ferret beyond 129 days since the first time antibodies were detected. This study reports for the first time the evidence of household pet ferrets exposure to SARS-CoV-2 in Spain to date.

Setaria digitata microfilaraemia in Mastomys coucha: an animal model for chemotherapeutic and immunobiological studies

Parasitology ◽  
1996 ◽  
Vol 113 (4) ◽  
pp. 323-330 ◽  
Author(s):  
S. Mukhopadhyay ◽  
A. P. Dash ◽  
B. Ravindran
Keyword(s):  
Animal Model ◽  
Body Weight ◽  
Oral Administration ◽  
Significant Positive Correlation ◽  
The Body ◽  
Setaria Digitata ◽  
Infective Larvae ◽  
Diethylcarbamazine Citrate ◽  
Gravid Females ◽  
Mastomys Coucha

SUMMARYIntraperitoneal implantation of adult gravid females of the bovine filarial parasite, Setaria digitata in Mastomys coucha was found to induce microfilaraemia lasting for about 125 days. The microfilariae (mf) could be detected as early as 4 days postimplantation (p.i.) and peak levels of about 30 mf in 20 µl of blood were observed by 21 days. A significant positive correlation was found between mf density and the body weight of recipients pre-implantation. The implanted adult worms were generally viable only for less than 1 week. Implantation resulted in a significant decrease in total leucocytes and erythrocytes, induction of eosinophilia, splenomegaly and anti-erythrocyte autoantibodies. The microfilariae in circulation developed into 3rd-stage infective larvae (L3) when fed onto Aedes aegypti (refm, Liverpool strain). The mf in circulation were found to be eliminated by oral administration of diethylcarbamazine citrate, indicating the usefulness of this model for screening potential anti-microfilarial drugs. During the microfilaraemic phase, priming with tetanus toxoid (TT) resulted in significantly decreased production of anti-toxin levels indicating a state of generalized immunosuppression. Induction of antibodies to various fractionated antigenic components of adult parasites could be demonstrated by enzyme immunoassay (EIA) in M. coucha implanted with live or cold-stunned adult worms. The S. digitata–M. coucha model thus is found amenable to perform chemotherapeutic and immunobiological investigations in experimental filariasis.

Reproductive biology of the Bigeye Thresher Shark, Alopias superciliosus (Lowe, 1939)

1992 ◽  
Vol 43 (1) ◽  
pp. 77 ◽  
Author(s):  
J Moreno ◽  
J Moron
Keyword(s):  
Gravid Female ◽  
Western Mediterranean ◽  
Embryo Morphology ◽  
The North ◽  
Western Mediterranean Sea ◽  
Eastern Atlantic Ocean ◽  
North Eastern ◽  
Imaginary Line ◽  
Thresher Shark ◽  
Gravid Females

More than 1000 specimens of Alopias superciliosus from fisheries exploited by the Spanish fishing fleet in the north-eastern Atlantic Ocean (from Cape SLo Vicente to the Ivory Coast) and in the western Mediterranean Sea (i.e. west of an imaginary line between Monaco and Algiers) were observed in discontinuous samples between 1981 and 1989. Ten gravid females, 20 fetuses and a large number of egg capsules were studied in detail. The embryo morphology provided evidence of ovophagy. Size at birth was probably at least 100 cm. Six males with total lengths of 400-410 cm exceeded the previously reported maximum length for this sex. The characteristics of the claspers and deferent ducts suggested a total length at maturity of about 276 cm for males. The smallest gravid female was 341 cm long. Also described are litter sizes, indications of reproductive seasonality, and the possible existence of a nursery area in waters off the south-western Iberian peninsula.

Coronaviren als Ursache einer systemischen granulomatösen Entzündung bei einem Frettchen (Mustela putorius furo)

2009 ◽  
Vol 37 (01) ◽  
pp. 40-44
Author(s):  
C. Maresch ◽  
A. Bracke ◽  
D. Bröker ◽  
A. Kirchhoff ◽  
J. P. Teifke
Keyword(s):  
Sich Eine ◽  
Mustela Putorius ◽  
Granulomatöse Entzündung ◽  
Mustela Putorius Furo

Zusammenfassung Gegenstand: Ein 3 Jahre altes weibliches Frettchen (Mustela putorius furo) wurde mit vergrößerten Kopflymphknoten bei ungestörtem Allgemeinbefinden vorgestellt. Im weiteren Verlauf kam es zu einer syste-mischen Schwellung der Körperlymphknoten und zu einer Vergrößerung der Milz bei zunehmender Verschlechterung des Allgemeinbefindens. Material und Methoden: Nach klinischer und ultrasonographischer Untersuchung wurden Feinnadelaspirate von Milz und Lymphknoten gewonnen und zytologisch untersucht. Die Milz und ein geschwollener Kniekehllymphknoten wurden entnommen und histopathologisch sowie immunhistologisch auf Gruppe-1-Coronavirusantigen untersucht. Ergebnisse: Zytopathologisch war eine reaktive Hyperplasie der vergrößerten Milz und des Lymphknotens nachweisbar. Histopathologisch fanden sich eine herdförmige pyogranulomatöse Splenitis sowie eine granulomatöse Lymphadenitis. Coronavirusantigen konnte in Makrophagen von Milz und Lymphknoten nachgewiesen werden. Schlussfolgerung: Als Ursache für die granulomatöse Entzündung wird die systemische Coronavirusinfektion der Frettchen (FSCV) angesehen, die vermutlich durch einen mit dem enteralen Coronavirus der Frettchen (FECV) eng verwandten Erreger hervorgerufen wird.

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