Associations of vitamin E intake and plasma α-tocopherol concentration with bone density status

The effect of supplementing dietary selenium (Se) and vitamin E was investigated in 330 24-week-old laying hens. The hens were fed a basal diet containing Se and α-tocopherol at 0.11 and 26 mg/kg, respectively, or a diet supplemented with Se at 0.3 mg/kg and vitamin E between 0 and 625 mg/kg. Se was supplied as Se-methionine or sodium selenite. The eggs were collected for analysis during the third, seventh and eleventh weeks of the experiment. Supplementation of either form of Se significantly increased the Se concentration in egg yolks and whites, with a more pronounced effect caused by Se-methionine. The egg yolk α-tocopherol concentration paralleled the dietary α-tocopherol concentration. At a high dietary α-tocopherol concentration (632 mg/kg), the retinol content in egg yolks from hens fed Se-methionine increased significantly. Supplementation of Se-methionine significantly increased the α-tocopherol content in the eggs in the third and seventh weeks of the experiment. A moderate decrease in yolk cholesterol was observed in hens fed Se-methionine and α-tocopherol at 119 mg/kg. The concentration of products from lipid peroxidation (thiobarbituric acid-reactive substances, TBARS) in egg yolks increased marginally during the refrigerated storage of the eggs for 2 weeks. The effect of dietary vitamin E on TBARS formation was generally small, although a more significant effect was observed at the highest dose tested.

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Alpha-tocopherol, an effective inhibitor of platelet adhesion

Blood ◽

10.1182/blood.v73.1.141.141 ◽

1989 ◽

Vol 73 (1) ◽

pp. 141-149 ◽

Cited By ~ 65

Author(s):

J Jandak ◽

M Steiner ◽

PD Richardson

Keyword(s):

Vitamin E ◽

Platelet Adhesion ◽

Ex Vivo ◽

Platelet Rich Plasma ◽

Flow Chamber ◽

Alpha Tocopherol ◽

Tocopherol Concentration ◽

Average Decrease ◽

Platelet Adherence

Abstract Platelet adhesiveness was tested ex vivo in a group of six normal individuals receiving varying doses of alpha-tocopherol. Adhesion to glass slides coated with fibronectin, collagen, fibrinogen, or plasma proteins was studied by perfusing platelet-rich plasma through a flow chamber that allowed time- and space-resolved observations of platelet adhesion. Platelet adherence was measured in an area of parallel flow lines and low shear rate under standardized conditions before and after dietary supplementation with vitamin E at doses of 200 and 400 IU/d. Platelet adherence differed in magnitude depending on the adhesive surface. There was a distinct preference of platelets to adhere to sites that had been previously occupied. A remarkable decrease in platelet adherence was observed after vitamin E supplementation. The average decrease in adhesion after 2 weeks of 200 IU vitamin E was 75%. After 2 weeks of 400 IU vitamin E, platelet adhesion was reduced by 82%. The inhibitory activity of alpha-tocopherol was dose dependent and correlated well with the increase in alpha-tocopherol concentration in platelets after supplementation. Scanning electron microscopy revealed a striking decrease of pseudopodium formation in alpha-tocopherol- enriched platelets. Our results suggest that vitamin E may also be an effective antiadhesive agent in vivo.

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Effect of dietary selenium on lipid oxidation, selenium and vitamin E content in the meat of broiler chickens

Czech Journal of Animal Science ◽

10.17221/358-cjas ◽

2008 ◽

Vol 53 (No. 7) ◽

pp. 306-311 ◽

Cited By ~ 15

Author(s):

M. Skřivan ◽

G. Dlouhá ◽

O. Mašata ◽

S. Ševčíková

Keyword(s):

Vitamin E ◽

Broiler Chickens ◽

Sodium Selenite ◽

Basal Diet ◽

Tocopherol Content ◽

Dietary Sodium ◽

Breast Meat ◽

Tocopherol Concentration ◽

Broiler Meat ◽

Dietary Treatments

An experiment was conducted to compare the effect of dietary sodium selenite and selenomethionine on selenium and α-tocopherol concentration in breast meat, oxidative stability of meat in broilers, and on growth performance, but only as an unimportant criterion in this case. Sexed broiler cockerels Ross 308 were allocated to 3 dietary treatments, each comprising 100 chickens. The basal diet was supplemented with 0 (control) or 0.3 mg/kg Se from sodium selenite (SS) or selenomethionine (SM). Dietary supplementation with SM increased (P < 0.05) body weight, but only by about 3%. Breast muscle Se concentration was increased (P < 0.05) by both Se sources, but more by SM (1.32 mg/kg dry matter; 0.47 mg/kg DM in control). The concentration of Se in excreta was 3 times higher with SS supplement than with SM supplement. Dietary Se supplementation increased (P < 0.05) the α-tocopherol content of breast meat from 25.9 mg/kg DM in the control to 33.2 mg/kg DM when SM supplementation was used. Furthermore, lipid peroxidation decreased compared to the control. The inclusion of SM in the diet reduced (P < 0.05) malondialdehyde (MDA) values in breast samples after 0, 3, and 5 days of cooler storage, whereas SS decreased (P < 0.05) the MDA of breast meat after 0 and 3 days of storage. The results of this experiment indicate that selenomethionine in the diet of broilers is capable of simultaneously increasing the content of selenium and vitamin E in broiler meat plus its stability in storage.

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α-Tocopherol concentration and stereoisomer composition in plasma and milk from dairy cows fed natural or synthetic vitamin E around calving

Journal of Dairy Research ◽

10.1017/s0022029906001701 ◽

2006 ◽

Vol 73 (2) ◽

pp. 227-234 ◽

Cited By ~ 42

Author(s):

Guillermo E Meglia ◽

Søren K Jensen ◽

Charlotte Lauridsen ◽

Karin Persson Waller

Keyword(s):

Vitamin E ◽

Dairy Cows ◽

Plasma Concentrations ◽

Dietary Treatment ◽

Tocopheryl Acetate ◽

Tocopherol Concentration ◽

Blood Concentrations ◽

Oral Supplementation ◽

Periparturient Period ◽

The Individual

The aim of this study was to compare the effects of supplementing dairy cows with 1000 IU/day of all-rac-α-tocopheryl acetate (SynAc), RRR-α-tocopheryl acetate (NatAc), or RRR-α-tocopherol (NatAlc), from approximately 3 weeks before estimated calving until 2 weeks after calving, on the concentration of α-tocopherol and its stereoisomers (RRR-, RSS-, RRS-, RSR- and the four 2S-forms of α-tocopherol) in blood and milk. An unsupplemented group was included as control. Blood samples were collected at 3, 2 and 1 weeks before estimated calving, at calving, and 3, 7 and 14 days after calving, while milk samples were taken twice within 24 h after calving and at 7 and 14 days in milk. Overall, time and treatment had significant effects on plasma α-tocopherol with higher concentrations in NatAc than in the other groups. In addition, SynAc had higher concentrations than Control, and NatAlc tended to be higher than Control. The lowest plasma concentrations were observed at calving and 3 days after calving. Independent of treatment, the concentration was higher in colostrum than in milk day 7 and 14 after calving. Analyses of the stereoisomer distribution in plasma and milk showed that, irrespective of dietary treatment, RRR-α-tocopherol was the most predominant form, constituting more than 86%, whereas the remaining part of α-tocopherol was made up by the three synthetic 2R isomers, while the 2S isomers only contributed less than 1% of the total α-tocopherol. In control cows and cows supplemented with natural vitamin E, the proportion of RRR-α-tocopherol in plasma and milk constituted more than 98% of the total α-tocopherol. In conclusion, the results indicate that daily oral supplementation of dairy cows with RRR-α-tocopheryl acetate gives the highest blood concentrations of α-tocopherol in the periparturient period. Analyses of the distribution of the individual stereoisomers of α-tocopherol further indicate that the bioavailability of RRR-α-tocopherol relative to synthetic stereoisomers in cattle is considerably higher than officially accepted until now.

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Alpha-tocopherol, an effective inhibitor of platelet adhesion

Blood ◽

10.1182/blood.v73.1.141.bloodjournal731141 ◽

1989 ◽

Vol 73 (1) ◽

pp. 141-149

Author(s):

J Jandak ◽

M Steiner ◽

PD Richardson

Keyword(s):

Vitamin E ◽

Platelet Adhesion ◽

Ex Vivo ◽

Platelet Rich Plasma ◽

Flow Chamber ◽

Alpha Tocopherol ◽

Tocopherol Concentration ◽

Average Decrease ◽

Platelet Adherence

Platelet adhesiveness was tested ex vivo in a group of six normal individuals receiving varying doses of alpha-tocopherol. Adhesion to glass slides coated with fibronectin, collagen, fibrinogen, or plasma proteins was studied by perfusing platelet-rich plasma through a flow chamber that allowed time- and space-resolved observations of platelet adhesion. Platelet adherence was measured in an area of parallel flow lines and low shear rate under standardized conditions before and after dietary supplementation with vitamin E at doses of 200 and 400 IU/d. Platelet adherence differed in magnitude depending on the adhesive surface. There was a distinct preference of platelets to adhere to sites that had been previously occupied. A remarkable decrease in platelet adherence was observed after vitamin E supplementation. The average decrease in adhesion after 2 weeks of 200 IU vitamin E was 75%. After 2 weeks of 400 IU vitamin E, platelet adhesion was reduced by 82%. The inhibitory activity of alpha-tocopherol was dose dependent and correlated well with the increase in alpha-tocopherol concentration in platelets after supplementation. Scanning electron microscopy revealed a striking decrease of pseudopodium formation in alpha-tocopherol- enriched platelets. Our results suggest that vitamin E may also be an effective antiadhesive agent in vivo.

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The evaluation of α-tocopherol concentration instead of α-tocopherol:cholesterol ratio in adult cystic fibrosis patients results in the overestimation of vitamin E deficiency

Journal of Medical Science ◽

10.20883/jms.313 ◽

2019 ◽

Vol 88 (1) ◽

pp. 21-25

Author(s):

Ewa Sapiejka ◽

Patrycja Krzyżanowska-Jankowska ◽

Dariusz Walkowiak ◽

Szymon Kurek ◽

Jarosław Walkowiak

Keyword(s):

Vitamin E ◽

Healthy Subjects ◽

High Performance ◽

Serum Vitamin ◽

Exocrine Pancreatic Function ◽

The Body ◽

Vitamin E Deficiency ◽

Tocopherol Concentration ◽

Cholesterol Ratio ◽

Cholesterol Levels

Introduction. It has been suggested that serum vitamin E concentrations in CF patients may not properly reflect the body resources of vitamin E. Therefore, we aimed to assess vitamin E status in CF adults relating it to reference values for healthy subjects, also taking into account the α‑tocopherol:cholesterol ratio. Material and Methods. The study group was composed of 33 CF patients − 18 (54.5%) females and 15 (45.5%) males − aged from 18 to 47 years. In all CF patients nutritional status and clinical expression of disease − lung function; Pseudomonas aeruginosa colonization; diabetes; cirrhosis; exocrine pancreatic function and vitamin E supplementation were analyzed. Vitamin E (α‑tocopherol) concentration was assessed by high‑performance liquid chromatography (HPLC).Resuls. CF adults have lower vitamin E concentrations (median: 830 µg/dl vs. 1132 µg/dl, p = 0.00174) and higher vitamin E: cholesterol ratio (median: 7.2 mg/g vs. 6.7 mg/g, p < 0.00001) than healthy subjects. The underlying factor that determines this phenomenon is related to low cholesterol levels observed in CF patients. The percentage of low vitamin E concentrations defined in comparison to healthy Polish peers is considerably higher than low vitamin E:cholesterol ratios (39.4% vs. 21.2%, p = 0.0011). Conclusions. The evaluation of α‑tocopherol concentration instead of α‑tocopherol:cholesterol ratio in CF adults results in the overestimation of vitamin E deficiency. Moreover, there is also potentially a large percentage of individuals with excessive vitamin E body resources. However, this aspect demands further studies.

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Effect of Vitamin E and A Supplementation on Egg Yolk α-tocopherol concentration

Poultry Science ◽

10.1093/ps/81.3.376 ◽

2002 ◽

Vol 81 (3) ◽

pp. 376-381 ◽

Cited By ~ 42

Author(s):

S. Grobas ◽

J. Méndez ◽

B.C. Lopez Bote ◽

B.C. De Blas ◽

G.G. Mateos

Keyword(s):

Vitamin E ◽

Egg Yolk ◽

Tocopherol Concentration

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The absorption of vitamin E is influenced by the amount of fat in a meal and the food matrix

British Journal Of Nutrition ◽

10.1079/bjn20041249 ◽

2004 ◽

Vol 92 (4) ◽

pp. 575-579 ◽

Cited By ~ 71

Author(s):

Yvonne M. Jeanes ◽

Wendy L. Hall ◽

Susan Ellard ◽

Elizabeth Lee ◽

John K. Lodge

Keyword(s):

Vitamin E ◽

Intervention Studies ◽

Tocopheryl Acetate ◽

Limited Information ◽

Healthy Individuals ◽

Food Matrix ◽

High Fat ◽

Low Fat ◽

Tocopherol Concentration ◽

Skimmed Milk

Vitamin E absorption requires the presence of fat; however, limited information exists on the influence of fat quantity on optimal absorption. In the present study we compared the absorption of stable-isotope-labelled vitamin E following meals of varying fat content and source. In a randomised four-way cross-over study, eight healthy individuals consumed a capsule containing 150 mg 2H-labelled RRR-α-tocopheryl acetate with a test meal of toast with butter (17·5 g fat), cereal with full-fat milk (17·5 g fat), cereal with semi-skimmed milk (2·7 g fat) and water (0 g fat). Blood was taken at 0, 0·5, 1, 1·5, 2, 3, 6 and 9 h following ingestion, chylomicrons were isolated, and 2H-labelled α-tocopherol was analysed in the chylomicron and plasma samples. There was a significant time (P<0·001) and treatment effect (P<0·001) in 2H-labelled α-tocopherol concentration in both chylomicrons and plasma between the test meals. 2H-labelled α-tocopherol concentration was significantly greater with the higher-fat toast and butter meal compared with the low-fat cereal meal or water (P<0·001), and a trend towards greater concentration compared with the high-fat cereal meal (P=0·065). There was significantly greater 2H-labelled α-tocopherol concentration with the high-fat cereal meal compared with the low-fat cereal meal (P<0·05). The 2H-labelled α-tocopherol concentration following either the low-fat cereal meal or water was low. These results demonstrate that both the amount of fat and the food matrix influence vitamin E absorption. These factors should be considered by consumers and for future vitamin E intervention studies.

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