scholarly journals Novel Use of Fluorescence Illumination with an Infrared Microscope

2000 ◽  
Vol 8 (2) ◽  
pp. 26-33 ◽  
Author(s):  
Thomas J. Tague ◽  
Lisa M. Miller
Keyword(s):  
Cell Biology ◽  
Manufacturing Industry ◽  
Optical Microscope ◽  
Infrared Analysis ◽  
Analysis Tool ◽  
Infrared Microspectroscopy ◽  
Nomarski Differential Interference Contrast ◽  
Sample Characterization ◽  
Infrared Microscope ◽  
Particle Contaminants

It has become increasingly obvious that infrared microspectroscopy can be the analysis tool of choice when determining the chemical composition of biological and biomedical samples. Frequently, fluorescence illumination is required for sample characterization, which previously required the use of a separate optical microscope. There has also been a need in the semiconductor manufacturing industry for a single tool for visualizing particle contaminants on integrated wafers as well as the ability to chemically determine their nature. There is now a single microscope platform for conducting rapid Nomarski differential interference contrast and fluorescence illumination sample visualization as well as infrared analysis. This novel infrared microscope has applicability to many fields of investigation, including pharmacology, forensics, cell biology, histology, gemology, and geology.

scholarly journals Evolving Strategies for the Incorporation of Bioinformatics Within the Undergraduate Cell Biology Curriculum

2003 ◽  
Vol 2 (4) ◽  
pp. 233-247 ◽  
Author(s):  
Jerry E. Honts
Keyword(s):  
Cell Biology ◽  
Biological Data ◽  
Point Of View ◽  
Analysis Tool ◽  
Undergraduate Biology ◽  
Biology Students ◽  
Introductory Course ◽  
Sequence Analysis Tool ◽  
Biology Curriculum ◽  
Simple Sequence

Recent advances in genomics and structural biology have resulted in an unprecedented increase in biological data available from Internet-accessible databases. In order to help students effectively use this vast repository of information, undergraduate biology students at Drake University were introduced to bioinformatics software and databases in three courses, beginning with an introductory course in cell biology. The exercises and projects that were used to help students develop literacy in bioinformatics are described. In a recently offered course in bioinformatics, students developed their own simple sequence analysis tool using the Perl programming language. These experiences are described from the point of view of the instructor as well as the students. A preliminary assessment has been made of the degree to which students had developed a working knowledge of bioinformatics concepts and methods. Finally, some conclusions have been drawn from these courses that may be helpful to instructors wishing to introduce bioinformatics within the undergraduate biology curriculum.

Applications of Microspectroscopy in the Near-Infrared Region

1989 ◽  
Vol 43 (5) ◽  
pp. 865-873 ◽  
Author(s):  
Matthew J. Smith ◽  
Richard T. Carl
Keyword(s):  
Near Infrared ◽  
Materials Science ◽  
Structural Information ◽  
Infrared Region ◽  
Small Samples ◽  
Infrared Analysis ◽  
Mid Infrared ◽  
Infrared Microspectroscopy ◽  
Different Types ◽  
Near Infrared Region

In this report, several applications of near-infrared microspectroscopy are illustrated using unmodified commercial instrumentation. The principal advantage of near-infrared microspectroscopy is the ability to analyze small samples which are totally absorbing in the mid-infrared region. Near-infrared analysis is shown to yield useful structural information about several different types of samples. Examples from the fields of materials science, single crystals, forensics and biological science are illustrated, and some tentative band assignments are made.

scholarly journals Pengaruh Perlakuan Panas Karburisasi Austemper pada Baja Laterit Paduan Cr-Mo terhadap Sifat Mekanik dan Struktur Mikro

2020 ◽  
Vol 10 (1) ◽  
pp. 19
Author(s):  
Satrio Herbirowo ◽  
Vinda Puspasari ◽  
M. Iqbal Primatama ◽  
Hendrik Hendrik ◽  
I Nyoman Gede Putrayasa Astawa ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Fracture Surface ◽  
Surface Characterization ◽  
Impact Test ◽  
Optical Microscope ◽  
Air Blowing ◽  
Sample Characterization ◽  
Cooling Media ◽  
Steel Heat

This research was aimed to do heat treatment of austemper carburization and investigate the effect of various cooling media on mechanical properties and microstructure of Cr-Mo alloy lateritic steel. Heat treatment was conducted to austenisation temperature at 950o C for 1 hour and austemper carburization at 400o C for 1 hour. Variation of cooling media included air blowing for 1 hour, water, and furnace cooling for 24 hours. Hardness and impact test were done using Hardness Rockwell and Charpy methods. Microstructure was observed using optical microscope. Fracture surface characterization was using SEM-EDX. The results showed the highest hardness of 65.48 HRC in sample that cooled by air blowing for 1 hour. The microstructure of this sample showed phases of ferrite, pearlite and martensite which causing higher hardness. The highest impact strength of 20 Joule took place in the furnace cooled sample. Characterization of the fracture surface using SEM-EDX showed dimple of ductile fractures.Penelitian ini bertujuan untuk melakukan proses perlakukan panas karburisasi austemper dan mempelajari pengaruh media pendinginan terhadap sifat mekanik dan struktur mikro baja laterit paduan Cr-Mo. Perlakuan panas yang dilakukan yaitu pemanasan sampel pada temperatur austenisasi (950o C) selama 1 jam dan proses karburisasi austemper dengan media serbuk arang halus pada temperatur 400o C selama 1 jam. Variasi pendinginan yang digunakan yaitu air blowing (semburan udara) selama 1 jam, air dan tungku selama 24 jam. Pengujian kekerasan dilakukan dengan metode Rockwell Hardness dan pengujian impak dilakukan dengan metode charpy. Karakterisasi struktur mikro dilakukan dengan proses metalografi dan mikroskop optik. Karakterisasi permukaan patahan pengujian impak dilakukan dengan SEM-EDX. Hasil penelitian ini menunjukkan nilai kekerasan tertinggi yaitu 65,48 HRC terjadi pada sampel dengan air blowing selama 1 jam. Struktur mikro sampel tersebut menunjukkan adanya fasa ferit, perlit dan martensit yang membuat sampel menjadi keras. Nilai kekuatan impak tertinggi sebesar 20 Joule terjadi pada sampel dengan pendinginan di dalam tungku selama 24 jam. Karakterisasi permukaan patahannya menggunakan SEM-EDX menunjukkan adanya dimple dari patah ulet.

scholarly journals Predictive Maintenance Through Thermographic Analysis: Case Study in a Manaus Industrial Pole Company

2019 ◽  
Vol 7 (11) ◽  
pp. 898-909
Author(s):  
Luã Platão Seabra Paiva ◽  
Livia Da Silva Oliveira ◽  
David Barbosa de Alencar ◽  
Paulo Oliveira Siqueira Júnior
Keyword(s):  
Data Collection ◽  
Manufacturing Industry ◽  
Predictive Maintenance ◽  
Analysis Tool ◽  
Electrical System ◽  
High Demand ◽  
Data Collection Process ◽  
Thermographic Analysis ◽  
Reduce Energy Consumption

Due to the high demand for electricity in the manufacturing industry, companies to obtain greater profitability on their produced goods, seek and adopt ways to reduce energy consumption, and use predictive maintenance as a tool by applying thermography. Thus, the purpose of the research is to show the importance of thermographic analysis for assessing losses and preserving the safety of the company's physical facilities. The research is descriptive, qualitative and case study. The instrument used for data collection were direct observation and document analysis. In this context, the results obtained were the mapping in the manufacturing facilities and the identification of some failures in the company's electrical system. After this data collection process, it was possible to analyze and plan the corrective actions. In conclusion, it is possible to reduce manufacturing costs through predictive maintenance through the thermographic analysis tool, positively impacting the company's financial results.

The Infrared Analysis of Polyethylene Terephthalate Fibers and of Their Strength as Related to Sample Preparation and to Particle Size

1989 ◽  
Vol 43 (5) ◽  
pp. 791-794 ◽  
Author(s):  
R. O. Carter ◽  
K. R. Carduner ◽  
M. C. Paputa Peck ◽  
D. H. Motry
Keyword(s):  
Particle Size ◽  
Sample Preparation ◽  
Polyethylene Terephthalate ◽  
Infrared Analysis ◽  
Spectral Features ◽  
Band Broadening ◽  
Small Particles ◽  
Large Particles ◽  
Local Sample ◽  
Infrared Microscope

An explanation is presented for the broadening of intense spectral features observed in the infrared spectra of polyethylene terephthalate fibers prepared as KBr pellets. Since band broadening occurs in the spectra of stronger, undegraded fiber, it is proposed that, the stronger the fiber, the more the difficulty one has in pulverizing the sample. Larger particles result from undegraded polyester, which lead to broader bands for intense features. The local sample pathlength in the region of the large particles is greater than that for small particles, which can be more homogeneously mixed. Fiber samples can also be presented to an infrared microscope either “as is” or flattened. Striking differences exist between spectra for flattened and round fibers that were otherwise similar.

scholarly journals Isotherm adsorption characteristics of carbon microparticles prepared from pineapple peel waste

2020 ◽  
Vol 5 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Asep Bayu Dani Nandiyanto ◽  
Gabriela Chelvina Santiuly Girsang ◽  
Rina Maryanti ◽  
Risti Ragadhita ◽  
Sri Anggraeni ◽  
...  
Keyword(s):  
Adsorption Process ◽  
Optical Microscope ◽  
Milling Process ◽  
Isotherm Models ◽  
Infrared Analysis ◽  
Adsorption Characteristics ◽  
Carbon Particles ◽  
Adsorption Analysis ◽  
Pineapple Peel ◽  
Isotherm Adsorption

The objective of this study was to investigate isotherm adsorption of carbon microparticles from pineapple peel waste. Carbon microparticles were prepared by carbonizing pineapple peel waste at 215-250°C and grinding using a saw-milling process. To investigate adsorption properties of carbon microparticles, experiments were done by evaluating adsorption of curcumin (as a model of adsorbate) in the ambient temperature and pressure under constant pH condition. To confirm the adsorption characteristics, carbon particles with different sizes (i.e., 100, 125, and 200 ?m) were tested, and the adsorption results were compared with several standard isotherm adsorption models: Langmuir, Freundlich, Temkin, and Dubinin- Radushkevich. To support the adsorption analysis, several characterizations (i.e., optical microscope, sieve test, and Fourier transform infrared analysis) were conducted. The adsorption test showed that the adsorption profile is fit to the Freundlich model for all variations, indicating the multilayer adsorption process on heterogeneous surfaces and interactions between adsorbate molecules. The results from other isotherm models also confirmed that the adsorption process occurs physically via Van der Waals force in binding adsorbate on the surface of adsorbent.

scholarly journals INFRARED SPECTROPHOTOMETRY ITS USE IN CHARACTERIZING HISTOCHEMICAL REACTION MECHANISMS

1966 ◽  
Vol 14 (11) ◽  
pp. 853-861 ◽  
Author(s):  
DAVID R. KVISTBERG
Keyword(s):  
Reaction Mechanisms ◽  
Infrared Analysis ◽  
Infrared Spectrophotometry ◽  
Histochemical Reaction ◽  
Model Compounds ◽  
In Situ Studies ◽  
Model Studies ◽  
Infrared Microscope

The technique of infrared analysis can be applied to histochemical and cytochemical problems. The approach outlined here illustrates the application to the mechanism of staining, starting with model compounds and tissue components. Future use of an infrared microscope employing improvements of modern electronics and optics should enable direct correlation of model studies with in situ studies.

scholarly journals A Comparison of Gene Expression Signatures from Breast Tumors and Breast Tissue Derived Cell Lines

2001 ◽  
Vol 17 (2) ◽  
pp. 99-109 ◽  
Author(s):  
Douglas T. Ross ◽  
Charles M. Perou
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Cell Lines ◽  
Cell Biology ◽  
Expression Patterns ◽  
Tumor Biology ◽  
Clinical Samples ◽  
Analysis Tool

Cell lines derived from human tumors have historically served as the primary experimental model system for exploration of tumor cell biology and pharmacology. Cell line studies, however, must be interpreted in the context of artifacts introduced by selection and establishment of cell linesin vitro. This complication has led to difficulty in the extrapolation of biology observed in cell lines to tumor biologyin vivo. Modern genomic analysis tool like DNA microarrays and gene expression profiling now provide a platform for the systematic characterization and classification of both cell lines and tumor samples. Studies using clinical samples have begun to identify classes of tumors that appear both biologically and clinically unique as inferred from their distinctive patterns of expressed genes. In this review, we explore the relationships between patterns of gene expression in breast tumor derived cell lines to those from clinical tumor specimens. This analysis demonstrates that cell lines and tumor samples have distinctive gene expression patterns in common and underscores the need for careful assessment of the appropriateness of any given cell line as a model for a given tumor subtype.

scholarly journals Correlation of organelle dynamics between light microscopic live imaging and electron microscopic 3D architecture using FIB-SEM

Microscopy ◽  
2020 ◽  
Author(s):  
Keisuke Ohta ◽  
Shingo Hirashima ◽  
Yoshihiro Miyazono ◽  
Akinobu Togo ◽  
Kei-ichiro Nakamura
Keyword(s):  
Electron Microscopy ◽  
Cell Biology ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Light And Electron Microscopy ◽  
Three Dimensional ◽  
Optical Microscope ◽  
Live Imaging ◽  
Reconstruction Technique ◽  
Electron Microscopic

Abstract Correlative light and electron microscopy (CLEM) methods combined with live imaging can be applied to understand the dynamics of organelles. Although recent advances in cell biology and light microscopy have helped in visualizing the details of organelle activities, observing their ultrastructure or organization of surrounding microenvironments is a challenging task. Therefore, CLEM, which allows us to observe the same area as an optical microscope with an electron microscope, has become a key technique in cell biology. Unfortunately, most CLEM methods have technical drawbacks, and many researchers face difficulties in applying CLEM methods. Here, we propose a live three-dimensional CLEM method, combined with a three-dimensional reconstruction technique using focused ion beam scanning electron microscopy tomography, as a solution to such technical barriers. We review our method, the associated technical limitations and the options considered to perform live CLEM.

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