Transmission FT-IR Chemical Imaging on Glass Substrates: Applications in Infrared Spectral Histopathology

2014 ◽  
Vol 86 (3) ◽  
pp. 1648-1653 ◽  
Author(s):  
Paul Bassan ◽  
Joe Mellor ◽  
Jonathan Shapiro ◽  
Kaye J Williams ◽  
Michael P. Lisanti ◽  
...  
Keyword(s):  
Chemical Imaging ◽  
Glass Substrates ◽  
Infrared Spectral ◽  
Ft Ir ◽  
Spectral Histopathology

FR-IR Molecular Microanalysis System

1990 ◽  
Vol 48 (2) ◽  
pp. 270-271
Author(s):  
John A. Reffner ◽  
William T. Wihlborg
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Integrated System ◽  
Morphological Examination ◽  
Fully Integrated ◽  
Ir Microscopy ◽  
Normal Functions ◽  
Infrared Microspectroscopy ◽  
Infrared Spectral ◽  
Ft Ir

The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.

scholarly journals Infrared spectral histopathology using haematoxylin and eosin (H&E) stained glass slides: a major step forward towards clinical translation

The Analyst ◽  
2017 ◽  
Vol 142 (8) ◽  
pp. 1258-1268 ◽  
Author(s):  
Michael J. Pilling ◽  
Alex Henderson ◽  
Jonathan H. Shanks ◽  
Michael D. Brown ◽  
Noel W. Clarke ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Clinical Translation ◽  
Great Promise ◽  
Stained Glass ◽  
Major Step ◽  
Glass Slides ◽  
Important Diagnostic Tool ◽  
Infrared Spectral ◽  
Spectral Histopathology

Infrared spectral histopathology has shown great promise as an important diagnostic tool, with the potential to complement current pathological methods.

scholarly journals Investigation of optical and photoelectric properties of poly (ohydroxyamide) sensitized by phthalocyanine as a perspective material for solar cells

2020 ◽  
Vol 220 ◽  
pp. 01019
Author(s):  
Elvira Fazalova ◽  
Konstantin Kochunov ◽  
Elena Bodyago ◽  
Georgii Konoplev ◽  
Nikolay Mukhin ◽  
...  
Keyword(s):  
Thin Films ◽  
Solar Radiation ◽  
Near Infrared ◽  
Wavelength Region ◽  
Organic Dye ◽  
Glass Substrates ◽  
Photoelectric Properties ◽  
Zinc Phthalocyanines ◽  
Infrared Spectral ◽  
Potential Use

Optical and photoelectric properties of poly (ohydroxyamide) (PHA) sensitized with zinc phthalocyanines were investigated in the visible and near infrared spectral regions. The structures were deposited on glass substrates by centrifugation and subsequent drying of a PHA film without thermal annealing. Optical spectra revealed characteristic absorption peaks of phthalocyanine in the longer wavelength region at 620-640 nm and 680-700 nm; absorption of the PHA matrix monotonously increases to the shorter wavelengths starting from 700 nm. Measurements of the photocurrent under irradiation with a high-power LED at a wavelength 630 nm showed photoconductivity related to the organic dye; photoconductivity also was observed while irradiated at 540 nm, presumably due to the absorption of PHA matrix. For non-sensitized (dye-free) PHA films no detectable photocurrents were produced by 630 nm irradiation. It was shown that introducing of phthalocyanines significantly improves optical absorption and photoconductivity of PHA thin films at the wavelengths, where the maximum in the spectral distribution of solar radiation lies. It was concluded that phthalocyanine-sensitized PHA has the potential use as a photosensitive organic material for solar applications, for example in developing composite organicinorganic structures with ferroelectrics.

scholarly journals Stain-less staining for computed histopathology

TECHNOLOGY ◽  
2015 ◽  
Vol 03 (01) ◽  
pp. 27-31 ◽  
Author(s):  
David Mayerich ◽  
Michael J. Walsh ◽  
Andre Kadjacsy-Balla ◽  
Partha S. Ray ◽  
Stephen M. Hewitt ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Clinical Practice ◽  
Clinical Pathology ◽  
Spectroscopic Imaging ◽  
Chemical Imaging ◽  
Tissue Composition ◽  
Alternative Approach ◽  
Ft Ir ◽  
Hematoxylin And Eosin ◽  
Ir Spectroscopic

Dyes such as hematoxylin and eosin (H&E) and immunohistochemical stains have been increasingly used to visualize tissue composition in research and clinical practice. We present an alternative approach to obtain the same information using stain-free chemical imaging. Relying on Fourier transform infrared (FT-IR) spectroscopic imaging and computation, stainless computed histopathology can enable a rapid, digital, quantitative and non-perturbing visualization of morphology and multiple molecular epitopes simultaneously in a variety of research and clinical pathology applications.

Infrared Spectral Signatures of Dust from Ground-Based FT-IR and Satellite in Anmyon, Korea

2009 ◽  
Author(s):  
Byung-Il Lee ◽  
Eun-Ha Sohn ◽  
Mi-Lim Ou ◽  
Kum-Lan Kim ◽  
Yoonjae Kim
Keyword(s):  
Spectral Signatures ◽  
Infrared Spectral ◽  
Ft Ir

Applications of AFM-IR—Diverse Chemical Composition Analyses at Nanoscale Spatial Resolution

2012 ◽  
Vol 20 (6) ◽  
pp. 16-21 ◽  
Author(s):  
Curtis Marcott ◽  
Michael Lo ◽  
Kevin Kjoller ◽  
Craig Prater ◽  
David P. Gerrard
Keyword(s):  
Ir Spectroscopy ◽  
Spatial Resolution ◽  
Chemical Characterization ◽  
Chemical Imaging ◽  
Polymer Materials ◽  
Small Samples ◽  
Chemical Information ◽  
Recent Developments ◽  
Ft Ir ◽  
Ir Microspectroscopy

The combination of infrared (IR) spectroscopy and atomic force microscopy (AFM) has produced a technique, called AFM-IR, which is becoming one of the most important recent developments in the field of IR spectroscopy and chemical imaging. Conventional Fourier transform infrared (FT-IR) microspectroscopy is well established as a technique for chemical characterization of small samples down to the 3–10 mm size range. This diffraction-imposed size limit has prevented the application of FT-IR microspectroscopy to smaller analysis regions that are relevant to analysis problems in polymer materials and the life sciences. The nanoIR™ instrument (Anasys Instruments, Santa Barbara, CA) described here uses an AFM probe as the IR absorbance sensor and hence breaks through the diffraction limit to attain spatial resolution improvements of between one and two orders of magnitude beyond previous techniques. Thus, the AFM-IR concept provides chemical information from nanoscale regions of polymers and other organic materials. This article describes the physics behind the technique, followed by results from several applications.

scholarly journals Label-free classification of colon cancer grading using infrared spectral histopathology

2016 ◽  
Vol 187 ◽  
pp. 105-118 ◽  
Author(s):  
C. Kuepper ◽  
F. Großerueschkamp ◽  
A. Kallenbach-Thieltges ◽  
A. Mosig ◽  
A. Tannapfel ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Label Free ◽  
Infrared Spectral ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
Free Classification ◽  
Cancer Grading ◽  
Spectral Histopathology ◽  
Good Agreement

In recent years spectral histopathology (SHP) has been established as a label-free method to identify cancer within tissue. Herein, this approach is extended. It is not only used to identify tumour tissue with a sensitivity of 94% and a specificity of 100%, but in addition the tumour grading is determined. Grading is a measure of how much the tumour cells differ from the healthy cells. The grading ranges from G1 (well-differentiated), to G2 (moderately differentiated), G3 (poorly differentiated) and in rare cases to G4 (anaplastic). The grading is prognostic and is needed for the therapeutic decision of the clinician. The presented results show good agreement between the annotation by SHP and by pathologists. A correlation matrix is presented, and the results show that SHP provides prognostic values in colon cancer, which are obtained in a label-free and automated manner. It might become an important automated diagnostic tool at the bedside in precision medicine.

scholarly journals Preliminary Phytochemical Analysis and Spectroscopic Analysis Methanolic Extract of Asteracantha Longifolia (Nees.)

2021 ◽  
pp. 80-87
Author(s):  
D. Veerakumar ◽  
M. Muthulingam
Keyword(s):  
Spectral Analysis ◽  
Fourier Transform ◽  
Spectroscopic Analysis ◽  
Methanolic Extract ◽  
Nitrogen Compounds ◽  
Phytochemical Analysis ◽  
Secondary Alcohols ◽  
Infrared Spectral ◽  
Ft Ir ◽  
Study Offer

The present investigation was focused on the preliminary phytochemical, UV-VIS spectrum and Fourier Transform Infrared Spectral analysis of Asteracantha longifolia. The Methanolic extract from the leaves of Asteracantha longifolia  were tested for the availability of alkaloids, flavonoids, tannins, saponins, proteins, Terpenoids, Phenols, Steroids, Glycosides and Carbohydrates. The UV-VIS profile demonstrated various peaks ranging from 400-700 nm with various Extract respectively. The FT-IR spectrum confirmed the presence of secondary alcohols, alkanes, alkynes, aromatics, alkenes, sulphur, phosphorus, esters, hydrocarbons, nitrogen compounds (amines) in methanolic extract. The results of this study offer a platform of using Asteracantha longifolia leaves as herbal alternative for various diseases including diabetic, cardiovascular etc. The results of this study offer a platform of using Asteracantha longifolia leaves as herbal alternative for various diseases including cancer and diabetic.

Sign in / Sign up

Export Citation Format

Share Document