Synthesis of Myrtenal-Based Nanocellulose/Diacylhydrazine Complexes with Antifungal Activity for Plant Protection

2021 ◽  
Author(s):  
Baoyu Li ◽  
Guishan Lin ◽  
Wengui Duan ◽  
Xiaoyu Wang ◽  
Bo Cen
Keyword(s):  
Antifungal Activity ◽  
Plant Protection

scholarly journals Protective activity of the fungal polysaccharides against fusariosis

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 617-619 ◽  
Author(s):  
A. Šrobárová ◽  
G. Kogan ◽  
L. Tamas ◽  
E. Machová
Keyword(s):  
Cell Wall ◽  
Antifungal Activity ◽  
Defense Mechanisms ◽  
Field Experiments ◽  
Plant Protection ◽  
Water Soluble ◽  
Protective Activity ◽  
Fresh Weight ◽  
Fungal Polysaccharides ◽  
Chitin And Chitosan

Most of the experiments carried out in the area of plant protection have used chitin and chitosan obtained from the crustacean chitin which production is rather expensive. In our study we have applied the chitin-glucan complex prepared from the waste mycelia of filamentous fungi, from baker’s yeast. Five different polysaccharides have been used for the preparation of water-soluble compounds and the assay of their antifungal activity against plant pathogen Fusarium oxysporum. In the field experiments, application of the polysaccharides led to the diminished infestation as well as to significantly increased productivity of fresh weight of the plants (tomato). The results demonstrated that application of the polysaccharides led to increased production of cell wall and some outher and intermembrane-bound proteins. Although the nature of the observed proteins has not been yet established, it can be speculated that they represent some enzymes involved in the antiinfective defense mechanisms in plants.

scholarly journals Application of Plant Extracts to Control Postharvest Gray Mold and Susceptibility of Apple Fruits to B. cinerea from Different Plant Hosts

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1430 ◽  
Author(s):  
Lina Šernaitė ◽  
Neringa Rasiukevičiūtė ◽  
Alma Valiuškaitė
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Plant Protection ◽  
Gray Mold ◽  
Postharvest Disease ◽  
Cinnamon Extract ◽  
Wide Range ◽  
Plant Hosts ◽  
Apple Fruits

Sustainable plant protection can be applied on apples against fungal pathogens such as Botrytis cinerea (which is responsible for gray mold)—a significant global postharvest disease. This pathogen can affect a wide range of hosts; and fruits may have variable susceptibilities to B. cinerea from different plant hosts. New possibilities to control gray mold in food production are under demand due to the emergence of resistance against antifungal agents in fungal pathogens. Cinnamon, pimento, and laurel extracts were previously assessed for antifungal activities under in vitro conditions and were found to have the potential to be effective against postharvest gray mold. Therefore, this study aimed to investigate the antifungal activity of cinnamon, pimento, and laurel extracts in vitro and against postharvest gray mold on apples to determine the susceptibility of apple fruits to B. cinerea from different plant hosts, and to analyze the chemical composition of the extracts. Apples (cv. “Connell Red”) were treated with different concentrations of extracts and inoculated with B. cinerea isolates from apple and strawberry followed by evaluation of in vitro antifungal activity. The results reveal that most of the concentrations of the extracts that were investigated were not efficient enough when assessed in the postharvest assay, despite having demonstrated a high in vitro antifungal effect. Apples were less susceptible to B. cinerea isolated from strawberry. To conclude, cinnamon extract was found to be the most effective against apple gray mold; however, higher concentrations of the extracts are required for the efficient inhibition of B. cinerea in fruits during storage.

scholarly journals Biotechnology for production of recombinant hybrid proteins from plants and microbes with antifungal activity

2020 ◽  
Author(s):  
E. Rogozin
Keyword(s):  
Antifungal Activity ◽  
Heterologous Expression ◽  
Plant Protection ◽  
Plant Protection Products ◽  
Hybrid Plant ◽  
Chimeric Proteins ◽  
Hybrid Proteins ◽  
Antimicrobial Polypeptides ◽  
Active Substances ◽  
Pathogenic Micromycetes

The principle of obtaining recombinant antimicrobial polypeptides from plant and microbial origins as a part of chimeric proteins with thioredoxin by heterologous expression in a prokaryotic system is presented. The results obtained in terms of their antifungal activity in relation to plant pathogenic micromycetes allow us to consider these compounds as prototypes of some active substances of environmentally friendly biofungicides, as well as possible components of hybrid plant protection products against fungal diseases.

scholarly journals Biosynthesis of components with antifungal activity against Aspergillus spp. using Streptomyces hygroscopicus

2015 ◽  
Vol 69 (2) ◽  
pp. 201-208
Author(s):  
Jelena Dodic ◽  
Zorana Roncevic ◽  
Jovana Grahovac ◽  
Bojana Bajic ◽  
Olivera Korolija
Keyword(s):  
Antifungal Activity ◽  
Soybean Meal ◽  
Plant Protection ◽  
Desirability Function ◽  
Inhibition Zone ◽  
Medium Composition ◽  
Streptomyces Hygroscopicus ◽  
Medium Components ◽  
Optimal Medium

Losses of apple fruit during storage are mainly caused by fungal phytopathogens. Traditionally, postharvest fungal disease is controlled by the application of synthetic fungicides. However, the harmful impact on environment as well as human health largely limits their application. To reduce these problems in agrochemicals usage, new compounds for plant protection, which are eco-friendly, should be developed. The aim of this study is optimization of medium composition in terms of glucose, soybean meal and phosphates content, by applying response surface methodology, for the production of agents with antifungal activity against Aspergillus spp. For biosynthesis was used strain of Streptomyces hygroscopicus isolated from the environment. Experiments were carried out in accordance with Box-Behnken design with three factors on three levels and three repetitions in the central point. Antifungal activity of the obtained cultivation mediums against Aspergillus oryzae and Aspergillus niger was determined, in vitro, using the diffusion - disc method. For determination optimal medium components desirability function was used. Achieved model predicts that the maximum inhibition zone diameter (40.93 mm) against test microorganisms is produced when the initial content of glucose, soybean meal and phosphates are 47.77 g/l, 24.54 g/l and 0.98 g/l, respectively. To minimize the consumption of medium components and costs of effluents processing, additional three sets of optimization were made. The chosen method for optimization of medium components was efficient, relatively simple and time and material saving. Obtained results can be used for the further techno-economic analysis of the process to select optimal medium composition for industrial application.

scholarly journals Reduced Graphene Oxide Nanosheet-Decorated Copper Oxide Nanoparticles: A Potent Antifungal Nanocomposite against Fusarium Root Rot and Wilt Diseases of Tomato and Pepper Plants

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 1001
Author(s):  
Sozan E. El-Abeid ◽  
Yosra Ahmed ◽  
José-Antonio Daròs ◽  
Mohamed A. Mohamed
Keyword(s):  
Graphene Oxide ◽  
Antifungal Activity ◽  
Copper Oxide ◽  
Reduced Graphene Oxide ◽  
Root Rot ◽  
Plant Protection ◽  
Crop Protection ◽  
Cuo Nps ◽  
Reduced Graphene ◽  
Pepper Plants

Sustainable use of nanotechnology in crop protection requires an understanding of the plant’s life cycle, potential toxicological impacts of nanomaterials and their mechanism of action against the target pathogens. Herein, we show some properties of a candidate antifungal nanocomposite made from copper oxide (CuO; otherwise an essential soil nutrient) nanoparticles (NPs), with definite size and shape, decorating the surface of reduced graphene oxide (rGO) nanosheets. The successful preparation of the rGO-CuO NPs was confirmed by spectroscopic and microscopic analyses, and its antifungal activity against wild strains of Fusarium oxysporum affecting tomato and pepper plants was successfully confirmed. A comparative analysis in vitro indicated that this nanocomposite had higher antifungal activity at only 1 mg/L than the conventional fungicide Kocide 2000 at 2.5 g/L. Further investigation suggested that rGO-CuO NPs creates pits and pores on the fungal cell membranes inducing cell death. In planta results indicated that only 1 mg/L from the nanocomposite is required to reduce Fusarium wilt and root rot diseases severity below 5% for tomato and pepper plants without any phytotoxicity for about 70 days. Comparatively, 2.5 g/L of Kocide 2000 are required to achieve about 30% disease reduction in both plants. The present study contributes to the concept of agro-nanotechnology, showing the properties of a novel ecofriendly and economic nanopesticide for sustainable plant protection.

scholarly journals STRAINS OF BACILLUS THURINGIENSIS WITH GROWTH-STIMULATING AND FUNGICIDAL ACTIVITY

2019 ◽  
Vol 48 (6) ◽  
pp. 5-12
Author(s):  
I. M. Gorobey ◽  
G. V. Kalmykova ◽  
N. V. Davydova ◽  
I. V. Andreeva
Keyword(s):  
Antifungal Activity ◽  
Bacillus Thuringiensis ◽  
Spring Wheat ◽  
Plant Protection ◽  
Antagonistic Activity ◽  
Wheat Seedlings ◽  
Pure Cultures ◽  
High Degree ◽  
Positive Effect ◽  
Spring Rape

The article presents the results of the study of growth-stimulating and antifungal activity of strainsBacillus thuringiensisssp.fukuokaensisssp.morrisoni, ssp.toumanoffi, ssp.dakotaand ssp.amagiensis. The positive effect of all studied strains on morphometric parameters of spring rape and spring wheat seedlings was revealed. Treatment of spring rape seeds with spore-crystal mixtures ofB. thuringiensiscontributed to a significant increase in the length of roots by 1.93.4 times and sprouts – by 1.3-1.9 times, depending on the strain. Stimulation of plant growth was also observed in the inoculation of spring wheat seeds with suspension strains ofB. thuringiensis. However, a significant positive effect was revealed only in the variant with the use of the strain ofB. thuringiensisssp.fukuokaensis. There was a downward trend in the number of spring wheat seedlings affected with root rot when the seeds were treated with the strains ofB. thuringiensisssp.morrisoni,ssp.dakota,ssp.amagiensis. In the experiments with pure cultures it was found that all tested strains ofB. thuringiensisshowed a high degree of antagonistic activity to two types of phytopathogens. The inhibitory activity of the strains ofB. thuringiensison the 10th day of the experiment with regard toFusarium oxysporumranged from 68 to 83%,Alternaria alternata– from 71 to 89%. The analysis showed that all studied strains ofB. thuringiensisstimulated the growth of plants of spring rape and spring wheat and showed antifungal activity. The obtained data are of interest for further research in order to create multifunctional biological preparations for plant protection.

scholarly journals The Multiple Activities and the Plant Beneficial Potential of Bacillus spp. Derived from the Permafrost of Western Siberia

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2347
Author(s):  
Olga V. Domanskaya ◽  
Nina A. Bome ◽  
Aleksandr V. Iashnikov ◽  
Anastasia V. Vasilchenko ◽  
Alexey S. Vasilchenko
Keyword(s):  
Antifungal Activity ◽  
First Generation ◽  
Western Siberia ◽  
Plant Protection ◽  
Treated Group ◽  
Climatic Conditions ◽  
Fusarium Spp ◽  
Cold Tolerant ◽  
Bacillus Spp ◽  
Biocontrol Potential

Agents of biological control are an important part of traditional agriculture, as well as organic farming. However, in the climatic conditions of countries that are located in cold and temperate regions, plant protection requires particular biocontrol agents that have adapted to environments with low and unstable temperatures. This work presents the biocontrol potential and plant-promoting activity of Bacillus spp. that was isolated from permafrost sediments in Western Siberia. It was found that all of the studied strains (n = 10) were able to produce indole-3-acetic acid (IAA) and chitinolytic enzymes at low positive temperatures (5 °C). The antifungal activity of cold-tolerant bacilli against Microdochium sp., Fusarium spp., and Alternaria sp was recorded. In greenhouse and field conditions, the selected strains (B. simplex 948 P1 (IAA-producing) and B. megaterium 312 (with antifungal activity)) were assessed in comparison to a commercially available fungicide (tebuconazole) and biofungicide (B.subtilis 26D). It was found that the bacilli in the seed germination assay exhibited low phytotoxicity and there was no significant advantage over the conventional fungicides in the yield stimulation assay. However, the twin consortia of B. megaterium 312 and B.subtilis 26D was able to increase winter wheat yields by 50% (compared to the untreated group), and by 70% (compared to the commercial biofungicide-treated group). Moreover, applying the twin consortia of Bacillus spp. significantly reduced the infection rate of Fusarium spp. in first-generation wheat grain.

scholarly journals ANTIFUNGAL ACTIVITY OF THE GENUS BACILLUSBACTERIA AS AGAINST THE PHYTOPATHOGEN ALTERNARIA SP.

2021 ◽  
pp. 40-43
Author(s):  
A.V. Malkova ◽  
◽  
A.N. Irkitova ◽  
D.Ye. Dudnik ◽  
Ye.N. Kargashilova ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Plant Protection ◽  
Active Part ◽  
Plant Protection Product ◽  
Microscopic Fungi ◽  
Block Method ◽  
Agar Block ◽  
Antifungal Effect ◽  
Alternaria Blight ◽  
Alternaria Sp

Alternaria blights caused by microscopic fungi of the Al-ternariagenus are widespread diseases of crops and or-namental plants. Russian and foreign scientists take an active part in developing biological products based on an-tagonistically active microorganisms. However, the situa-tion regarding Alternaria blight is still unfavorable. The re-search goal was to study the antifungal activity of the ge-nus Bacillusstrains against the phytopathogen Alternaria sp. The agar block method was used to determine the an-tagonistic activity of 8 rhizospheric bacilli strains (B. pu-milus4, B. pumilus5, B. pumilus6, B. pumilus7, B. licheni-formis8, B. licheniformis9, B. licheniformis10, and B. pu-milus16). All investigated strains showed themselves as antagonists in relation to Alternaria. On the 14th day of the experiment, the diameter of the Alternaria sp. in the control was 85.83 ± 8.78 mm. The following values of the phyto-pathogen mycelium diameter were recorded in dishes with bacilli: B. pumilus4 -10.00 ± 0.87 mm, B. pumilus 5-12.17 ± 0.76 mm, B. pumilus 6-11.33 ± 1.26 mm, B. pu-milus7 -8.00 ± 3.00 mm, B. pumilus16 -7.67 ± 0.29 mm. Alternariasp. did not grow beyond the 5 mm diameter block with all B. licheniformisstrains. Bacteria of the B. licheniformisspecies had a more pronounced antifungal effect (100%) than the B. pumilusstrains (91.13-96.70%). B. licheniformis8, 9, 10 and B. pumilus16, 7, 4 strains are primarily recommended for inclusion in a biological plant protection product against Alternariablight

scholarly journals Study on antifungal activity of silver/bentonite nanomaterials on soybean phytopathogenic fungi

2018 ◽  
Vol 15 (2) ◽  
pp. 349-357
Author(s):  
Nguyễn Hoài Châu ◽  
Nguyễn Thị Thúy ◽  
Đào Trọng Hiền ◽  
Hoàng Thị Mai ◽  
Nguyễn Văn Quang ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antifungal Activity ◽  
Plant Protection ◽  
Chemical Reduction ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Soybean Plant ◽  
Subsequent Formation ◽  
Inhibition Effect ◽  
Sclerotial Germination

In the present study, silver nanoparticles were synthesized by chemical reduction method route into the lamellar space of bentonite (Ag/CTS/Bentonite). Silver nitrate (AgNO3) was taken as a metal precursor, sodium borohydride (NaBH4) as a reducing agent, reduction of Ag+ ions and the subsequent formation of Ag nanoparticles, chitosan as a natural polymeric stabilizer and was sticked silver nanoparticles to the surface of bentonite particles, respectively. Manipulating the size and shape of Ag/CTS/Bentonite nanomaterial was characterized using transmission electron microscopy (TEM), spherical silver nanoparticles, as depicted by TEM, were found to have a wide particle size distribution from 5 nm to 90 nm. Meanwhile, the X-Ray flourescence (XRF) spectrum indicated the presence of silver on bentonite particles. Antifungal activity of the synthesized Ag/CTS/Bentonite nanomaterial was investigated against crop pathogenic fungi (Fusarium oxysporium (F. oxysporium) and Rhizoctonia solani (R. solani)) isolated from infected soybean plant in Bac Ninh province by the Plant Protection Research Institute. The assessment of fungicidal activity of the Ag/CTS/Bentonite nanomaterial showed that this product exhibited strong antifungal activity towards soybean pathogenic fungi. At highest nanosilver concentration of the Ag/CTS/Bentonite nanocomposite (400 ppm) sclerotial germination of F. oxysporum was almost inhibited, after 7 days the inhibition effect on sclerotial germination attained 66.70%. For the case of R. solani, after 2 days at 400 ppm silver nanoparticles concentration the inhibition effect on sclerotial germination attained 92.82%. The obtained results suggested that the synthesized Ag/CTS/Bentonite nanomaterial acts as an effective antifungal agent. Thus, it could be used in developing novel antifungal agents for potential applications in agriculture.

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