Complex Micellization Behavior of the Polysorbates Tween 20 and Tween 80

2021 ◽  
Author(s):  
Hannah Knoch ◽  
Maximilian H. Ulbrich ◽  
Judith J. Mittag ◽  
Julia Buske ◽  
Patrick Garidel ◽  
...  
Keyword(s):  
Tween 80 ◽  
Tween 20 ◽  
Micellization Behavior

scholarly journals FORMULATION AND CHARACTERIZATION OF LAFUTIDINE NANOSUSPENSION FOR ORAL DRUG DELIVERY SYSTEM

2018 ◽  
Vol 10 (2) ◽  
pp. 20 ◽  
Author(s):  
Noor Mohammed Dawood ◽  
Shaimaa Nazar Abdal-hammid ◽  
Ahmed Abbas Hussien
Keyword(s):  
Particle Size ◽  
Fourier Transforms ◽  
Oral Drug Delivery ◽  
In Vitro Release ◽  
Tween 80 ◽  
Precipitation Method ◽  
Oral Drug ◽  
Tween 20 ◽  
Dissolution Profile ◽  
Particle Size Reduction

Objective: The objective of this study was to prepare nanosuspension of a practical water insoluble antiulcer drug which is lafutidine to enhance the solubility, dissolution rate with studying the effect of different formulation variables to obtain the best formula with appropriate physical properties and higher dissolution rate.Methods: Nanosuspension of lafutidine was prepared using solvent anti-solvent precipitation method using Polyvinylpyrrolidone K-90(PVP K-90) as the stabilizer. Ten formulations were prepared to show the effect of different variables in which two formulations showed the effect of stabilizer type, three formulations showed the effect of stabilizer concentration, two formulations showed the effect of combination of polymer with surfactant such as tween 80, three formulations show the effect of stirring speed and three formulations prepare to show the effect of addition of co-surfactant such as tween 20. All these formulations are evaluated for their particle size and entrapment efficiency and in vitro release. The selected one was evaluated for zeta potential, scanning electron microscope, atomic force microscopy, Fourier transforms infrared spectroscopy, differential scanning calorimetry, saturation solubility and stability study.Results: The formulations (F3-F10) were in the nano size. The optimum concentration of the stabilizer was in the formulation when the drug: polymer: surfactant ratio 1:4:4 and the optimum stirring speed was 1500 rpm. Dramatic effect on the particle size reduction was found by the addition of co-surfactant (tween 20) in formulation F7 that has a particle size 15.89±1.8 nm. The selected formula F7 showed an enhanced dissolution profile (10 min) compared to the pure drug at all-time intervals.Conclusion: The results show that the formulation that contains drug: PVP-K90: tween 80: tween 20 in ratio 1:4:2:2 is the best one and can be utilized to formulate lafutidine nanosuspension. 

scholarly journals FORMULATION AND EVALUATION OF ATORVASTATIN CALCIUM NANOCRYSTALS CONTAINING P-GLYCOPROTEIN INHIBITORS FOR ENHANCING ORAL DELIVERY

2021 ◽  
pp. 19-23
Author(s):  
SARAH LABIB ◽  
MOHAMED NASR ◽  
MOHAMED NASR
Keyword(s):  
Polyethylene Glycol ◽  
Oral Drug Delivery ◽  
Tween 80 ◽  
Degree Of Crystallinity ◽  
Oral Drug ◽  
Tween 20 ◽  
Atorvastatin Calcium ◽  
Peg 400 ◽  
P Glycoprotein ◽  
Saturated Solubility

Objective: The main objective of this study was to develop atorvastatin calcium (ATR) as an oral drug delivery system for a P-glycoprotein (P-gp) substrate drug using different pharmaceutical excipients that inhibit P-glycoprotein and evaluate the influence of nanocrystals on the dissolution characteristics and bioavailability compared to the plain drug. Methods: A nanosuspension was prepared by Solvent-antisolvent precipitation method using a solvent containing stabilizer that act as a p-gp inhibitor dissolved in distilled water as polyethylene glycol 300, polyethylene glycol 400 (PEG 300, PEG 400), tween 20 and tween 80 while the solvent selected for atorvastatin calcium was methanol. The concentrations were as follows: PEG 300 and 400 = 0.25% w/v, tween 20 and 80 = 0.75% v/v. Nanocrystals were extracted from the suspension and characterized. Results: Particle size of the drug was 1307±127.79 nm while the formulas prepared ranged from 223±17.67 to 887±58.12 nm. Pure ATR had a saturated solubility of 0.059±0.005 mg/ml and the prepared nanocrystals ranged from 0.32±0.021 to 0.88±0.019 mg/ml. The Percentage of drug released of plain atorvastatin calcium reached 41.49% while the formula ranged from 44.32 to 61.5%. Both XRD and SEM discussed the degree of crystallinity as follows: F1<F2<F4<F3<ATR. Conclusion: 0.3% of PEG 300 and PEG 400 were not enough to formulate proper nanocrystals while 0.75% tween 20 and tween 80 achieved acceptable formulas. F4 which is prepared with tween 80 exhibited the highest enhancement in saturated solubility, dissolution rate and subsequently expected to have improved oral bioavailability.

scholarly journals ENHANCED ENZYMATIC ACTIVITY OF STREPTOMYCES GRISEOPLANUS L-ASPARGINASE VIA ITS INCORPORATION IN AN OIL-BASED NANOCARRIER

2020 ◽  
pp. 203-210
Author(s):  
ABEER A. EL-HADI ◽  
HANAN MOSTAFA AHMED ◽  
RANIA A. ZAKI ◽  
AMIRA MOHAMED MOHSEN
Keyword(s):  
Thermal Stability ◽  
Oleic Acid ◽  
Enzymatic Activity ◽  
Specific Activity ◽  
Residual Activity ◽  
Tween 80 ◽  
Biochemical Properties ◽  
Lymphocytic Leukemia ◽  
Tween 20 ◽  
Ph Range

Objective: L-asparaginase (L-asp) is a vital enzyme used as a therapeutic agent in combination with other drugs in the treatment of acute lymphoma, melanosarcoma and lymphocytic leukemia. Immobilization of enzymes through loading on nanoemulsion (NE) results in some advantages such as enhancing their stability and increasing their resistance to proteases. Aim of the present study is to formulate L-asp loaded nanoemulsion to enhance its efficiency and thermal stability. Methods: Nanoemulsion loaded with L-asp crude extract (specific activity 13.23U/mg protein) was prepared employing oleic acid as oil, tween 20/tween 80 as surfactants and propylene glycol (PG) as co-surfactant. L-asp loaded NE underwent several thermodynamic stability studies and the optimized formulae were further examined for their biochemical properties and thermal stability. Results The developed formulations were spherical in shape and their sizes were in the nanometric dimensions with negatively charged zeta potential values. Upon comparing the enzyme activity of L-asp loaded NE employing tween 20 (F1) or tween80 (F4) at different concentrations, the results revealed that F4 NE showed higher enzymatic activity [323 U/ml] compared to F1 NE [197 U/ml] at the same concentration. The nanosized immobilized L-asp was more stable in the pH range from 8 to 8.5 as compared to free L-asp. The immobilized enzyme preserved about 59.11% of its residual activity at 50 °C; while free L-asp preserved about 33.84%. Conclusion: In the view of these results, NE composed of oleic acid, tween 80 and PG represents a promising dosage form for enhancing the activity and stability of Streptomyces griseoplanus L-asp.

Solubility Studies and Validation of Lovastatin using High Performance Liquid Chromatography Method

2021 ◽  
pp. 6285-6288
Author(s):  
Nurhabibah Nurhabibah ◽  
A.K. Nugroho ◽  
Ronny Martien ◽  
Endang Lukitaningsih
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Oleic Acid ◽  
High Performance ◽  
Tween 80 ◽  
Tween 20 ◽  
Chromatography Method ◽  
Peg 400 ◽  
Solubility Studies ◽  
Liquid Chromatography Method

This study aimed to determine the solubility of lovastatin (LV) in different oil, surfactant, and co-surfactant using the high-performance liquid chromatography method. LV was solubility studies in different vehicle. The different vehicle used almond oil, sunflower oil, oleic acid, olive oil, soybean oil, and corn oil, isoprophyl myristate, myoglyol, tween 80, tween 20, and cremophor R.H. 40, propylene glycol, and PEG 400. Each of them was added lovastatin until saturated. The mixtures were mixing, sonicating, putting in the water bath and standing for 24 hours, then centrifugated. Each of the aliquot 2 µL diluted with acetonitrile and determination of concentration lovastatin using HPLC, with detector ultraviolet at 237 nm. Before determinate LV validated, and curve calibration at range 2-16 µg/mL was made. This study using the HPLC method with detector UV 237 nm, Agilent C 18 (4.6 x 150 mm 5 µ) column, and acetonitrile: water (70:30 v/v) as mobile phase. Calibration curve of lovastatin at the range 2-16 µg/mL with linear regression 0.999. Accuracy and precision showed that. Lovastatin has high soluble in oleic acid, tween 80, and PEG 400.

scholarly journals Satureja montana L. Essential Oils: Chemical Profiles/Phytochemical Screening, Antimicrobial Activity and O/W NanoEmulsion Formulations

Pharmaceutics ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 7 ◽  
Author(s):  
Alessandro Maccelli ◽  
Luca Vitanza ◽  
Anna Imbriano ◽  
Caterina Fraschetti ◽  
Antonello Filippi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Atmospheric Pressure Chemical Ionization ◽  
Tween 80 ◽  
Tween 20 ◽  
Volatile Fraction ◽  
Hydrodynamic Diameter ◽  
Ion Cyclotron Resonance ◽  
Ft Icr ◽  
Satureja Montana

Chemical fingerprints of four different Satureja montana L. essential oils (SEOs) were assayed by an untargeted metabolomics approach based on Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) coupled with either electrospray ionization or atmospheric pressure chemical ionization ion sources. Analysis and relative quantification of the non-polar volatile fraction were conducted by gas chromatography (GC) coupled to MS. FT-ICR MS confirmed significant differences in the polar metabolite composition, while GC-MS analyses confirmed slight fluctuations in the relative amount of major terpenes and terpenoids, known to play a key role in antimicrobial mechanisms. Oil in eater (O/W) nanoemulsions (NEs) composed by SEOs and Tween 20 or Tween 80 were prepared and analyzed in terms of hydrodynamic diameter, ζ-potential and polydispersity index. The results confirm the formation of stable NEs homogeneous in size. Minimum inhibitory and minimum bactericidal concentrations of SEOs were determined towards Gram-positive (Listeria monocytogenes, Staphylococcus aureus, Staphylococcus haemolyticus) and Gram-negative clinical isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Serratia marcescens). Commercial SEO showed strongest antibacterial activity, while SEO 3 was found to be the most active among the lab made extractions. MIC and MBC values ranged from 0.39 to 6.25 mg·mL−1. Furthermore, a SEO structured in NEs formulation was able to preserve and improve antimicrobial activity.

Surfactants for the Effective Recovery of Salmonella in Fatty Foods

1982 ◽  
Vol 45 (3) ◽  
pp. 249-252 ◽  
Author(s):  
J.-Y. D'AOUST ◽  
C. MAISHMENT ◽  
P. STOTLAND ◽  
A. BOVILLE
Keyword(s):  
Salmonella Enteritidis ◽  
Brilliant Green ◽  
False Negative ◽  
Tween 80 ◽  
Food Samples ◽  
Nutrient Broth ◽  
Tween 20 ◽  
Negative Results ◽  
False Negative Results ◽  
Tween 60

Inhibitory concentrations of 8 surfactants were determined for Salmonella typhimurium and Salmonella enteritidis. Pure culture work resulted in the exclusion of Tween 20, Teepol 610 and Brij 35 and retention of Tergitol-7 (T-7), Tween 80 (TW 80), Triton X-100 (TX), Myrj 52S (M), and Arlacel 80 + Tween 60 (AT) for a study on the quantitative recovery of Salmonella in 45 naturally contaminated fatty foods. Replicate food samples (100 g) were preenriched overnight at 35 C in nutrient broth supplemented with 3%(w/v) surfactant except AT (10%). Serial dilutions of preenrichment cultures were selectively enriched overnight in tetrathionate brilliant green (43 C) and selenite cystine (35 C) broths and streaked on bismuth sulfite and brilliant green sulfa agar media. Recovery with all test surfactants was comparable to that obtained with nutrient broth controls; of 270 preenrichment cultures tested, only 7 false-negative results attributable to TX (3), AT (2), M (1), and nutrient broth control (1) were obtained. None of the surfactants consistently yielded greater populations of Salmonella for given foods or food categories; median counts for preenrichment cultures were 104–105 salmonellae/ml for low and high moisture foods and 106–107 salmonellae/ml for animal feeds. These results suggest that use of surfactants to facilitate detection of Salmonella in fatty foods is not warranted.

scholarly journals Enhanced Production of Ligninolytic Enzymes by a Mushroom Stereum ostrea

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
K. Y. Usha ◽  
K. Praveen ◽  
B. Rajasekhar Reddy
Keyword(s):  
Solid State ◽  
Copper Sulphate ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Tween 80 ◽  
Veratryl Alcohol ◽  
White Rot ◽  
Tween 20 ◽  
Laccase Production ◽  
Enhanced Production

The white rot fungi Stereum ostrea displayed a wide diversity in their response to supplemented inducers, surfactants, and copper sulphate in solid state fermentation. Among the inducers tested, 0.02% veratryl alcohol increased the ligninolytic enzyme production to a significant extent. The addition of copper sulphate at 300 μM concentration has a positive effect on laccase production increasing its activity by 2 times compared to control. Among the surfactants, Tween 20, Tween 80, and Triton X 100, tested in the studies, Tween 80 stimulated the production of ligninolytic enzymes. Biosorption of dyes was carried out by using two lignocellulosic wastes, rice bran and wheat bran, in 50 ppm of remazol brilliant blue and remazol brilliant violet 5R dyes. These dye adsorbed lignocelluloses were then utilized for the production of ligninolytic enzymes in solid state mode. The two dye adsorbed lignocelluloses enhanced the production of laccase and manganese peroxidase but not lignin peroxidase.

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