Avenacin, an Antimicrobial Substance Isolated from Avena sativa. I. Isolation and Antimicrobial Activity*

Biochemistry ◽  
1964 ◽  
Vol 3 (3) ◽  
pp. 424-426 ◽  
Author(s):  
J. V. Maizel ◽  
H. J. Burkhardt ◽  
H. K. Mitchell
2020 ◽  
Vol 2 ◽  
pp. 00002
Author(s):  
Dyah Fitri Kusharyati ◽  
Pancrasia Maria Hendrati ◽  
Dini Ryandini ◽  
Tsani Abu Manshur ◽  
Meilany Ariati Dewi ◽  
...  

<p class="Abstract"><i>Bifidobacterium</i> is a group of Lactic Acid Bacteria (LAB) that commonly found in the gastrointestinal tract and vagina. LAB has many health benefits, such as produce an antimicrobial substance against a pathogen. This research aims to isolate <i>Bifidobacterium</i> from an infant’s feces and know its antimicrobial activity against <i>Escherichia coli</i> and <i>Candida albicans.</i> A total of 5 isolates <i>Bifidobacterium</i> spp. were isolated from the sample. <span lang="EN">The largest inhibitory activity against <i>E. coli</i> was shown by isolate Bb3F, with the inhibitory zone of 10.80 mm. While the largest inhibition activity against <i>C. albicans</i> was shown by isolate Bb1B and Bb3F with the inhibitory zone of 9.70 mm.</span><o:p></o:p></p>


Biochemistry ◽  
1964 ◽  
Vol 3 (3) ◽  
pp. 426-431 ◽  
Author(s):  
H. J. Burkhardt ◽  
J. V. Maizel ◽  
H. K. Mitchell

2020 ◽  
Vol 6 (3) ◽  
pp. 295-297
Author(s):  
Sebastian Buhl ◽  
J. Vogt ◽  
A. Stich ◽  
R. Brückner ◽  
C. Bulitta

AbstractThe risk of healthcare associated infections (HAI) is rising with the utilization of more complex medical devices. Cleaning and disinfecting measures of such devices are often insufficient leading to an increased microbiological contamination on these devices. Recent studies imply that antimicrobial coatings could present a solution for this topic. In this work a novel approach for the introduction of an antimicrobial technology into plastic granulate was tested. After 3-D printing the antimicrobial activity of the test samples was analysed. Our results show that the integration of an antimicrobial substance to ABS plastic is feasible only with sophisticated plastic processing technologies. Simple heating or mixing of the substance did not allow integration of the antimicrobial substance into the 3-D printed sample, but it was possible to integrate the antimicrobial ingredient into the raw material by compounding. The printed test samples showed strong antimicrobial activity in the standardized test procedures.


2007 ◽  
Vol 0 (0) ◽  
pp. 071003002748001-??? ◽  
Author(s):  
S. Bahraminejad ◽  
R. E. Asenstorfer ◽  
I. T. Riley ◽  
C. J. Schultz

2016 ◽  
Vol 4 (2) ◽  
pp. 140
Author(s):  
Evy Yulianti ◽  
Anna Rakhmawati ◽  
Kartika Ratna Pertiwi

The aims of this study was to investigate the effect of medium with different pH (6,7,9), salt concentration (0,5; 1; 2 %) and fermentation periode (24 and 48 hr) to the antimicrobial activity of cell free extract to three pathogens, Eschericia coli, Staphylococcus aureus, and Candida albican. This study consists of antimicrobial compounds production with different medium and continued by antimicrobial tested to fungi and bacterial pathogens Eschericia coli, Staphylococcus aureus, and Candida albican by Kirby Bauer  method with paper disk. Different salt concentration, pH and fermentation periode affected the  antimicrobial activity potency of cell free extract yielded by thermophylic bacteria. Treatment which yielded CFE with the best antimicrobial activity was treatment with 24 hr fermentation, pH 7 and salt concentration 2% to S aureus and pH 6 salt concentration 1% to E coli. Cell free extract had no potency as antifungi to Candida albicans except CFE yielded by thermophylic bacteria fermented in medium with pH 7 and salt concentration 1% in 24 hr with inhibition zone index 1,17. Keywords: cell free extract, antimicrobial, pH, salt concentration, fermentation period


2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Emma Letcher ◽  
Davida S. Smyth

Honey has a stable physiochemical composition that contributes to its long shelf life and has been noted as an antimicrobial substance for centuries. Although it is common knowledge that honey affords some antimicrobial properties, the specific mechanisms behind this remain elusive. This paper hypothesizes that the microorganisms in certain raw honeys contribute towards their antimicrobial properties. In our study, we analyzed several raw and processed honey samples to determine their microbial constituents. The antimicrobial potential of the isolated microbes was tested using several clinically relevant bacteria including Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa. Of the tested honeys, Manuka (New Zealand) and Wildflower honey (Tennessee, USA) contained microorganisms exhibiting antimicrobial activity. All the isolated colonies grew on MacConkey and Mannitol agar and generated bands for the 16S rRNA gene implying that they were bacteria. This paper concludes that bacteria isolated from honey could be a new area of research within the topic of antimicrobial honey samples. 


2014 ◽  
Vol 11 (2) ◽  
pp. 1062-1066
Author(s):  
Baghdad Science Journal

The present study investigated the total content of phenolic compounds, antioxidant and antimicrobial activities of water extracts oat (Avena sativa) and basil (Ocimum basilicum), medicinal plants. The Folin-ciocalteu reagent assay was used to estimate the total phenolic content of plants extract. The antioxidant capacity of the plants extract was tested by ferric reducing/antioxidant power Assay (FRAP) and ferric reducing scavenging activity using DPPH method, and the antimicrobial activity was measured against [Staphylococcus epidermidis; Staphylococcus aureus; Proteus spp.; Klebsiella spp.; Escherichia coli; Candida albicans] as tester strains. The total phenolic content of Avena sativa and Ocimum basilicum extracts revealed that the mixture of plants showed higher content. The mixture of Avena sativa and Ocimum basilicum extracts showed the highest antioxidant capacity followed by Avena sativa extract and Ocimum basilicum extract with FRAP and DPPH assay. However, mixture of Avena sativa and Ocimum basilicum extracts exhibited the highest antimicrobial activity when compared to the other extracts. Thus the study revealed that the consumption of mixture herbs may enhance the immune power of our body against diseases due to free radicals.


2018 ◽  
Vol 14 (4) ◽  
pp. 713-720
Author(s):  
Phan Thi Hong Thao ◽  
Nguyen Vu Mai Linh ◽  
Nguyen Thi Hong Lien ◽  
Nguyen Van Hieu

Endophytic actinomycetes colonize in living plant tissues without causing harm to the host plant. In fact, they are regarded as effective bio-control agents and plant growth promoters due to their ability to activate plant self-immunity and produce biologically active secondary metabolites. Recent studies reported higher rates in finding new strains and antimicrobial substances among endophytes than actinomycetes from soil and plant surface. In this work, endophytic actinomycetes were isolated from Ham Yen orange trees, a famous specialty of Tuyen Quang province, and screened for antimicrobial activity against plant pathogenic bacteria and fungi. The isolate TQR8-14, showing strong activity, was studied with regard to its biological and taxonomical characteristics and production of antimicrobial substance. Based on the mycelial morphology, this isolate was placed in yellow group of streptomycetes. The isolate developed milky to yellow aerial mycelia on all test media and long straight spore chains bearing smooth cylinder spores. The isolate could grow at wide range of temperature 15 to 45oC, of pH 4 to 10; on substrates such as carboxy methyl cellulose, starch, protein and chitin; and tolerated up to 3% salinity. Its 16S rDNA nucleotide sequence (1,404 bp) shared 99% similarity to Streptomyces parvus. Therefore, the isolate was named as Streptomyces parvus TQR8-14. The highest antimicrobial activity was achieved in culture on medium AH4 containing soybean meal and glucose, at 30oC and pH 7.0.


2011 ◽  
Vol 76 (5) ◽  
pp. 699-707 ◽  
Author(s):  
Svetlana Seatovic ◽  
Jelena Jovanovic-Novakovic ◽  
Gordana Zavisic ◽  
Zeljka Radulovic ◽  
Marija Gavrovic-Jankulovic ◽  
...  

The aim of this study was the partial characterization of the antimicrobial peptide bacteriocin G2 produced by probiotic bacteria Lactobacillus plantarum G2, which was isolated from a clinical sample of a healthy person. Antimicrobial substance was secreted in the supernatant of an L. plantarum G2 culture, and showed a diverse spectrum of antimicrobial activity of all the tested strains of the genera Lactobacillus and the pathogenic bacteria Staphylococcus aureus and Salmonella ?bony. Isoelectric focusing revealed that bacteriocin G2 is a cationic peptide (pI about 10) with a molecular mass of 2.2 kDa according to tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis, SDS-PAGE. The antimicrobial activity of bacteriocin G2 was diminished by the proteolytic action of trypsin and proteinase K. Bacteriocin G2 preserved its biological activity in the temperature range 40-60?C (15 min), which was lost at 80?C. Bacteriocin G2 was stable in the pH range 2-9, while treatment with 1 % Tween 80 and 1 % urea resulted in increased antimicrobial activity. The probiotic strain L. plantarum G2 produces the antimicrobial substance proteinaceous in nature with bacteriocin characteristics. Bacteriocin production is one of the key properties of probiotic bacteria with clinical potential as anti-infective agents, which will increase the likelihood of its in vivo efficacy.


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