scholarly journals In Vitro Studies Evaluating Leaching of Mercury from Mine Waste Calcine Using Simulated Human Body Fluids

2010 ◽  
Vol 44 (12) ◽  
pp. 4782-4788 ◽  
Author(s):  
John E. Gray ◽  
Geoffrey S. Plumlee ◽  
Suzette A. Morman ◽  
Pablo L. Higueras ◽  
James G. Crock ◽  
...  
Keyword(s):  
Human Body ◽  
Mine Waste ◽  
Body Fluids ◽  
In Vitro Studies

Analysis of tribological behavior of medical-grade UHMW polyethylene under dry and lubricated conditions with human body fluids using Taguchi and GRA techniques

2020 ◽  
pp. 089270572094190
Author(s):  
Omar Hussain ◽  
Babar Ahmad ◽  
Shahid Saleem
Keyword(s):  
Synovial Fluid ◽  
Human Body ◽  
Tribological Behavior ◽  
Normal Load ◽  
Ultrahigh Molecular Weight Polyethylene ◽  
Tribological Performance ◽  
Body Fluids ◽  
Optimization Techniques ◽  
Pin On Disc

The present work focuses on evaluating the tribological behavior of ultrahigh molecular weight polyethylene (UHMWPE) with 316L stainless steel and Ti6Al4V under dry and lubricated sliding conditions using human body fluids (synovial fluid and human serum). Eighteen trials of experiments were performed using a reciprocating sliding tribometer (pin-on-disc) at 37°C based on Taguchi’s L18 (21 × 32) array. The in-vitro experimental results revealed that UHMWPE offers better tribological performance under synovial fluid lubrication conditions irrespective of the counterface material. The optimization of the response variables (coefficient-of-friction (COF) and specific wear rate (WR)) was performed using optimization techniques (Taguchi and Grey relational analysis). It was revealed that Ti6Al4V counterface material under synovial fluid lubrication and normal load of 52 N exhibits the optimal tribological performance with UHWMPE. The contribution of process parameters on the COF and WR was evaluated using analysis of variance. It was established that load is the most significant parameter, affecting COF and WR.

scholarly journals The Pharmaceutical Availability of Gambier Leaves Bioactive Fraction Coated Tablet in Simulated Human Body Fluids

2018 ◽  
pp. 94-101
Author(s):  
Indah Sulistyowati ◽  
Nanang Yunarto ◽  
Nurul Aini ◽  
Kelik M Arifin
Keyword(s):  
Traditional Medicine ◽  
Human Body ◽  
Body Fluids ◽  
Dissolution Profile ◽  
Dissolution Medium ◽  
Atherosclerotic Lesions ◽  
Coated Tablet ◽  
Bioactive Fraction ◽  
The Stability

The widespread usage of traditional medicine either in the developed or developing countries, makes traditional medicine requirements become a major concern in terms of assuring the safety and effectiveness of treatment. WHO suggested dissolution study to support traditional medicine clinical trials. The catechins from Gambier could reduce atherosclerotic lesions case caused by elevated levels of cholesterol, LDL, and triglycerides. Catechin is hygroscopic that becoming unstable. Raising the stability, Gambier is made to a coated tablet. This study aimed to determine the condition of gambier leaves bioactive fraction coated tablet in simulated human body fluids through an in vitro testing using dissolution tester. Three formulations coated tablet with different coating percentage had been tested using dissolution tester apparatus. The test was conducted in water, acid, and buffer as dissolution medium to generate the dissolution profile. Tablet evaluation showed that the three formulations dissolved 71.25% ± 6.26 to 91.05% ±3.05 in the water, acid, and buffer dissolution medium. The gambier leaves bioactive fraction coated tablet had more than 70% pharmaceutical availability in simulated human body fluids.

scholarly journals Lytic Bacteriophages against Mutidrug-Resistant Klebsiella pneumoniae: Development of an Effective Phage-based Approach to Combat Multidrug Resistance

2021 ◽  
Author(s):  
Willames Martins ◽  
Juliana Cino ◽  
Mei Li ◽  
Michael Lenzi ◽  
Kirsty Sands ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Human Body ◽  
Galleria Mellonella ◽  
Body Fluids ◽  
Highly Active ◽  
The United Kingdom ◽  
Phage Cocktail ◽  
Potential Use

Abstract This study aimed to develop a phage-based approach against Klebsiella pneumoniae sequence type (ST16). Phages were investigated using sewage samples from Brazil, Bangladesh, Saudi Arabia, Thailand and the United Kingdom. After isolation, the bacteriophages were submitted to microbiological, structural and genomic characterisation. The best phages were selected to integrate a phage-cocktail for potential use in humans. In vitro and in vivo experiments were performed to demonstrate the efficiency of this approach using a collection of 56 clinical strains of K. pneumoniae ST16 with distinct genetic backgrounds. Anti-biofilm activity, synergism with meropenem and activity in human body fluids were also evaluated. Fourteen lytic phages were isolated, belonging to Autographiviridae, Ackermannviridae, Demerecviridae, Drexlerviridae, and Myoviridae families. The viruses demonstrated good activity against our collection of K. pneumoniae ST16 at a different range of temperatures but also against other important Klebsiella clones such as ST11, ST15, and ST258. The cocktail Katrice-16 was highly active in vitro against K. pneumoniae ST16 collection consisting of isolates from several disparate countries. It demonstrated good in vivo activity in the Galleria mellonella model, anti-biofilm and synergic activity with meropenem. In addition, we also showed the Katrice-16 activity in human body fluids. Our results reinforce how effective bacteriophages can be, supporting their capacity for human clinical use to combat prevalent antimicrobial resistance bacterial clones.

scholarly journals Metabolome of Exosomes: Focus on Vesicles Released by Cancer Cells and Present in Human Body Fluids

2019 ◽  
Vol 20 (14) ◽  
pp. 3461 ◽  
Author(s):  
Aneta Zebrowska ◽  
Agata Skowronek ◽  
Anna Wojakowska ◽  
Piotr Widlak ◽  
Monika Pietrowska
Keyword(s):  
Cancer Cells ◽  
Human Body ◽  
Culture Media ◽  
Cell Communication ◽  
Diagnostic Value ◽  
Body Fluids ◽  
Model Systems ◽  
Cell Phenotype ◽  
Shed Light

Exosomes and other classes of extracellular vesicles (EVs) have gained interest due to their role in cell-to-cell communication. Knowledge of the molecular content of EVs may provide important information on features of parental cells and mechanisms of cross-talk between cells. To study functions of EVs it is essential to know their composition, that includes proteins, nucleic acids, and other classes biomolecules. The metabolome, set of molecules the most directly related to the cell phenotype, is the least researched component of EVs. However, the metabolome of EVs circulating in human blood and other bio-fluids is of particular interest because of its potential diagnostic value in cancer and other health conditions. On the other hand, the metabolome of EVs released to culture media in controlled conditions in vitro could shed light on important aspects of communication between cells in model systems. This paper summarizes the most common approaches implemented in EV metabolomics and integrates currently available data on the composition of the metabolome of EVs obtained in different models with particular focus on human body fluids and cancer cells.

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production

In Vitro Studies on the Mechanism of the Antifibrino-lytic Action of E-Aminocaproic Acid (EACA)

1968 ◽  
Vol 19 (03/04) ◽  
pp. 584-592 ◽  
Author(s):  
Hanna Lukasiewicz ◽  
S Niewiarowski
Keyword(s):  
Aminocaproic Acid ◽  
Test Tube ◽  
In Vitro Studies ◽  
Lytic Action ◽  
Human Plasminogen ◽  
Experimental Findings ◽  
Fibrin Clots

Summary and Conclusion1. It has been found that EACA does not inhibit activation of human plasminogen into plasmin by SK and UK in a concentration of 5 × 10–2 M. The activation of bovine plasminogen by SK and UK is inhibited by this concentration of EACA but not by a lower one.2. EACA in concentrations of 1,5 × 10–1 – 10–4 M does not inhibit casein proteolysis by plasmin. The proteolysis of fibrinogen and fibrin measured by the release of TCA soluble tyrosine is inhibited by EACA in concentrations of 1,5 × 10–1 – 10–2 M.3. The lysis of non-stabilized clots by plasmin measured in a test tube was inhibited by an EACA concentration of 5 × 10–3 – 5 × 10–4 M. The lysis of stabilized clots by plasmin was inhibited by an EACA concentration of 10–5 M.4. On the basis of experimental findings and data given in literature the authors postulate that the mechanism of the antifibrinolytic effects of EACA consists mainly in a modification of plasmin action on fibrin. These effects are dependent on the structure of the fibrin clots.

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