Positive Surface Charge Enhances Selective Cellular Uptake and Anticancer Efficacy of Selenium Nanoparticles

2012 ◽  
Vol 51 (16) ◽  
pp. 8956-8963 ◽  
Author(s):  
Bo Yu ◽  
Yibo Zhang ◽  
Wenjie Zheng ◽  
Cundong Fan ◽  
Tianfeng Chen
Keyword(s):  
Surface Charge ◽  
Cellular Uptake ◽  
Selenium Nanoparticles ◽  
Anticancer Efficacy ◽  
Positive Surface Charge

Design and Characterization of a Cancer-Targeted Drug Co-Delivery System Composed of Liposomes and Selenium Nanoparticles

2020 ◽  
Vol 20 (9) ◽  
pp. 5295-5304
Author(s):  
Guangshan Xuan ◽  
Min Zhang ◽  
Yang Chen ◽  
Shan Huang ◽  
Imshik Lee
Keyword(s):  
Folic Acid ◽  
Zeta Potential ◽  
Cellular Uptake ◽  
Delivery System ◽  
Mtt Assay ◽  
Hela Cells ◽  
Selenium Nanoparticles ◽  
Spherical Particles ◽  
Anticancer Efficacy ◽  
Targeted Drug

A drug co-delivery system composed of selenium nanoparticles (SeNPs) has attracted increasing interest due to its ability to increase the anticancer efficacy against multidrug-resistant cancer cells. In this study, a cancer-targeted drug co-delivery system combining fluorescein-loaded liposomes and SeNPs was designed and evaluated. The system was developed by coating SeNPs and fluorescein-loaded liposomes with folic acid-chitosan conjugates (FA-CS-SeNPs-Lips). Folic acid-chitosan conjugates (FA-CS) were synthesized by coupling folic acid (FA) with chitosan (CS), and the structure was confirmed by performing Fourier transform spectroscopy (FT-IR) and nuclear magnetic resonance (1H-NMR) spectroscopy. Dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM) were used to evaluate the particle size, Zeta potential, and morphology. The cytotoxicity of SeNPs coated with FA-CS conjugates (FA-CS-SeNPs) toward A549 cells and HeLa cells was examined using the MTT assay. The cancer-targeting ability and drug release behaviors were evaluated in vitro by measuring the cellular uptake of fluorescein and dialysis, respectively. The FA-CS-SeNPs were uniform, spherical particles with a ~50 nm diameter and high positive Zeta potential (+57.7 mV). Based on the results of the MTT assay, FA-CS-SeNPs displayed a more significant increase in the anticancer efficacy in HeLa cells than CS-SeNPs. FA-CS-SeNPs-Lips not only slowly released fluorescein but also specifically targeted HeLa cells through selective binding between folate and folate receptors to increase the cellular uptake of fluorescein.

scholarly journals Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles

2012 ◽  
pp. 835 ◽  
Author(s):  
Tianfeng Chen ◽  
Yang ◽  
Tang ◽  
Zhong ◽  
Bai ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Selenium Nanoparticles ◽  
Anticancer Efficacy ◽  
Surface Decoration

Rationally designed curcumin laden glycopolymeric nanoparticles: Implications on cellular uptake and anticancer efficacy

2020 ◽  
Vol 137 (32) ◽  
pp. 48954
Author(s):  
N. Naga Malleswara Rao ◽  
Shipra Sharma ◽  
Krushna Kaduba Palodkar ◽  
Veera Sadhu ◽  
Manu Sharma ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Anticancer Efficacy

scholarly journals EFFECTS OF POLYMERIC NANOPARTICLE SURFACE PROPERTIES ON INTERACTION WITH BRAIN TUMOR ENVIRONMENT

Nano LIFE ◽  
2013 ◽  
Vol 03 (04) ◽  
pp. 1343003 ◽  
Author(s):  
BRANDON MATTIX ◽  
THOMAS MOORE ◽  
OLGA UVAROV ◽  
SAMUEL POLLARD ◽  
LAUREN O'DONNELL ◽  
...  
Keyword(s):  
Human Brain ◽  
Surface Charge ◽  
Cellular Uptake ◽  
Drug Clearance ◽  
Cellular Interaction ◽  
Normal Tissues ◽  
Poly Ethylene ◽  
Uptake Studies ◽  
Effect Of Surface

Current chemotherapy treatments are limited by poor drug solubility, rapid drug clearance and systemic side effects. Additionally, drug penetration into solid tumors is limited by physical diffusion barriers [e.g., extracellular matrix (ECM)]. Nanoparticle (NP) blood circulation half-life, biodistribution and ability to cross extracellular and cellular barriers will be dictated by NP composition, size, shape and surface functionality. Here, we investigated the effect of surface charge of poly(lactide)-poly(ethylene glycol) NPs on mediating cellular interaction. Polymeric NPs of equal sizes were used that had two different surface functionalities: negatively charged carboxyl ( COOH ) and neutral charged methoxy ( OCH 3). Cellular uptake studies showed significantly higher uptake in human brain cancer cells compared to noncancerous human brain cells, and negatively charged COOH NPs were uptaken more than neutral OCH 3 NPs in 2D culture. NPs were also able to load and control the release of paclitaxel (PTX) over 19 days. Toxicity studies in U-87 glioblastoma cells showed that PTX-loaded NPs were effective drug delivery vehicles. Effect of surface charge on NP interaction with the ECM was investigated using collagen in a 3D cellular uptake model, as collagen content varies with the type of cancer and the stage of the disease compared to normal tissues. Results demonstrated that NPs can effectively diffuse across an ECM barrier and into cells, but NP mobility is dictated by surface charge. In vivo biodistribution of OCH 3 NPs in intracranial tumor xenografts showed that NPs more easily accumulated in tumors with less collagen. These results indicate that a robust understanding of NP interaction with various tumor environments can lead to more effective patient-tailored therapies.

scholarly journals Characterization of Liposomes for Cancer Cell Transfection

2007 ◽  
Vol 1 (1) ◽  
pp. 60-63
Author(s):  
Svetlana A Tatarkova ◽  
Satvinder Khaira
Keyword(s):  
Surface Charge ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Surface Potential ◽  
Cell Transfection ◽  
Similar Extent ◽  
Positive Surface Charge ◽  
Negative Surface ◽  
Subsequent Addition

We have characterized a broad range of liposome formulations with varying DcChol:DOPE ratio. Subsequent addition of DcChol to liposomes increases its positive surface charge. However, loading the nuclear acids did not neutralize the overall negative surface potential to a similar extent. The liposomes were tested by transfection of DNA in living cancer cells.

scholarly journals Effect of Surface Charge on the Cellular Uptake and Cytotoxicity of Fluorescent Labeled Cellulose Nanocrystals

2010 ◽  
Vol 2 (10) ◽  
pp. 2924-2932 ◽  
Author(s):  
Khaled A. Mahmoud ◽  
Jimmy A. Mena ◽  
Keith B. Male ◽  
Sabahudin Hrapovic ◽  
Amine Kamen ◽  
...  
Keyword(s):  
Surface Charge ◽  
Cellular Uptake ◽  
Cellulose Nanocrystals ◽  
Effect Of Surface

scholarly journals The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy

2018 ◽  
Vol 9 (23) ◽  
pp. 5260-5269 ◽  
Author(s):  
Di Zhang ◽  
Lin Wei ◽  
Meile Zhong ◽  
Lehui Xiao ◽  
Hung-Wing Li ◽  
...  
Keyword(s):  
Surface Charge ◽  
Optical Microscopy ◽  
Cellular Uptake ◽  
Single Particle ◽  
Cellular Internalization ◽  
Uptake Efficiency ◽  
Size And Shape ◽  
Internalization Process ◽  
Cellular Uptake Efficiency

The cellular uptake efficiency of nanostructures has been demonstrated to be highly dependent on the surface charge, size and shape although the cellular internalization process is still far from being well-understood.

Electrostatic-driven solid phase microextraction coupled with surface enhanced Raman spectroscopy for rapid analysis of pentachlorophenol

2017 ◽  
Vol 9 (3) ◽  
pp. 459-464 ◽  
Author(s):  
Weiwei Bian ◽  
Sha Zhu ◽  
Mingying Qi ◽  
Lanlan Xiao ◽  
Zhen Liu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Charge ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Surface Enhanced Raman Spectroscopy ◽  
Rapid Analysis ◽  
Positive Surface Charge ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Ag Substrate

Rapid analysis of pentachlorophenol by electrostatic-driven SPME–SERS on a nanoporous Ag substrate with positive surface charge.

HYALURONIC ACID-DOCETAXEL CONJUGATE LOADED NANOLIPOSOMES FOR TARGETING TUMOR CELLS

2020 ◽  
pp. 88-99
Author(s):  
MULUNEH FROMSA SEIFU ◽  
LILA KANTA NATH ◽  
DEBASHIS DUTTA
Keyword(s):  
Hyaluronic Acid ◽  
Glial Cells ◽  
Cellular Uptake ◽  
Drug Loading ◽  
Microscopic Investigation ◽  
In Vitro Cytotoxicity ◽  
Scanning Calorimetry ◽  
Anticancer Efficacy ◽  
Apoptotic Effect

Objective: Docetaxel (DTX), a potent anticancer drug, is suffering from non-specificity and drug resistance as major limitations. In this investigation, we developed Hyaluronic acid (HA)-Docetaxel conjugate (HA-DTX) loaded nanoliposomes to target cancer cells via passive and active targeting approaches. Methods: HA-DTX was synthesized and characterized by UV-Visible spectrophotometry, FT-IR spectroscopy, 1H NMR spectroscopy, Differential scanning calorimetry and X-ray diffraction and then loaded into nanoliposomes (L-NLs) by thin-film hydration method. L-NLs were characterized physicochemically and evaluated for anticancer efficacy by in vitro cytotoxicity study in glioma cells (C6 glial cells); cellular uptake and apoptotic effect were investigated by fluorescence microscopy. Results: HA-DTX was successfully synthesized; L-NLs had an average size of 123.0±16.53 nm, polydispersity index of 0.246±0.01 and zeta potential of 44.4±6.79 mV. Also, L-NLs exhibited 90.54%±4.22 of drug loading efficiency and 2.68%±0.12 of drug loading, releasing about 57.72%±1.17 at pH 5.2 and only 14.14%±1.32 at pH 7.4 after 48 h. No significant change instability was observed after storage at 5 °C±3 °C as well as at 25 °C±2 °C/60% RH±5% RH for 6 mo. The cytotoxicity effect of L-NLs was higher by 10% that of marketed formulation at 10 µg/ml docetaxel concentration. Fluorescence microscopic investigation showed that more cellular uptake and apoptotic effect were observed in L-NLs treated C6 glial cells than in those treated with the marketed formulation. Conclusion: HA-DTX loaded nanoliposomes enabled docetaxel to target C6 glial cells with better efficacy and might be effective to treat glioma.

scholarly journals Tumor-acidity activated surface charge conversion of two-photon fluorescent nanoprobe for enhanced cellular uptake and targeted imaging of intracellular hydrogen peroxide

2019 ◽  
Vol 10 (40) ◽  
pp. 9351-9357 ◽  
Author(s):  
Lanlan Chen ◽  
Shuai Xu ◽  
Wei Li ◽  
Tianbing Ren ◽  
Lin Yuan ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Surface Charge ◽  
Cellular Uptake ◽  
Targeted Imaging ◽  
Two Photon ◽  
Fluorescent Nanoprobe ◽  
Tumor Acidity ◽  
Ph Dependent ◽  
Intracellular Hydrogen Peroxide ◽  
Activated Surface

A smart, two-photon fluorescent GC–NABP nanoprobe with pH-dependent surface charge conversion was developed for tumor-targeted visualization of H2O2.

Sign in / Sign up

Export Citation Format

Share Document