Determination of Ergosterol Levels in Barley and Malt Varieties in the Czech Republic via HPLC

2008 ◽  
Vol 56 (11) ◽  
pp. 4092-4095 ◽  
Author(s):  
Lenka Jedličková ◽  
David Gadas ◽  
Pavla Havlová ◽  
Josef Havel
2021 ◽  
Vol 37 (37) ◽  
pp. 22-42
Author(s):  
Alicja Paluch ◽  
Henryk Spustek

The ever-increasing need for in-depth analysis and quantification of the national power, in particular ‘hard’ and ‘soft’ power-generating factors as well as difficulties in identifying a comprehensive and effective method for scientific determination of the national power, have given rise to research in the indicated scientific issues within this article. The presented considerations aim to define the assumptions for a descriptive sub-model that would enable a comparison of Poland’s power in the economic sphere (which is a component of the non-military sphere) with the power of selected European countries. The research hypothesis is that, among the variety of descriptive variables in the economic sphere of the national power, there is a subset of mutually independent variables, at the same time strongly correlated with the national power, which make it possible to define assumptions for the sub-model of the national power. The steps of the research procedure were carried out using the method of system analysis (multi-criteria comparative analysis) and statistical analysis. The research activities undertaken have shown that the factors that are strongly correlated with the national power in the economic area of the European countries adopted for the analysis are: dynamics of industrial production, private sector credit flows and economic freedom index. The comparative analysis carried out demonstrates that the greatest increase in the economic power in the analysed period took place in Germany (0.68). Slightly smaller growth was recorded in the Czech Republic (0.62) and Poland (0.60), while the lowest value of increase was in Romania (0.23). The conducted qualitative comparative analysis of the economic power of selected European countries allowed to conclude that the independent variables identified are crucial for the formation of the economic power of the analysed countries. At the same time, a fairly strong position of the Czech Republic and Poland in relation to the economic power of Germany was found. The performed quantification of the economic power of the European countries provides a basis for the correct determination of changes in the power distribution of political units, assessment of the power and resources held by the state.


Author(s):  
Hana Mlejnkova ◽  
Katerina Sovova ◽  
Petra Vasickova ◽  
Vera Ocenaskova ◽  
Lucie Jasikova ◽  
...  

The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.


2008 ◽  
Vol 25 (No. 4) ◽  
pp. 214-220 ◽  
Author(s):  
J. Simonová ◽  
M. Vázlerová ◽  
I. Steinhauserová

In this study, the pathogenic <i>Y. enterocolitica</i> of serotype O:3 was monitored. The serotype is widely spread in Europe and has been linked to human yersiniosis. For the detection of pathogenic strains were used biochemical and serological methods as well as PCR methods based on the identification of virulence genes (<i>ail</i>, <i>rfbC</i>, <i>ystA</i>, <i>yadA</i>, <i>virF</i>). The occurrence of <i>Y. enterocolitica</i> O:3 strains was monitored in slaughter animals from a number of farms in the Czech Republic. A total of 3748 samples were collected coming from pigs (1388), cattle (633), poultry (902), and slaughter facilities (825). Fifty-two <i>Y. enterocolitica</i> O:3 isolates were identified by biochemical and serologic methods, and 53 <i>Y. enterocolitica</i> O:3 isolates were identified by PCR methods (46 isolates from pigs, 2 isolates from poultry, 3 isolates from cattle, and 2 isolates from a poultry slaughtering facility). All isolates of <i>Y. enterocolitica</i> O:3 carried genes <i>ail</i> and <i>rfbC</i>, 83% isolates carried gene <i>ystA</i>, 79% isolates carried gene <i>yadA</i> and 49% isolates carried gene <i>virF</i>. The use of PCR methods based on the identification of <i>ail</i> and <i>rfbC</i> genes provides for a sufficiently specific identification of pathogenic <i>Y. enterocolitica</i> O:3 strains with optimum time consumption compared to biochemical and serological methods. It is not recommendable to use other PCR methods (detection of the <i>ystA, <i>yadA</i>, and <i>virF</i> genes) for the detection of pathogenic <i>Y. enterocolitica</i> strains because those methods are not very specific for the determination of pathogenicity.


2008 ◽  
Vol 53 (No. 9) ◽  
pp. 421-431 ◽  
Author(s):  
K. Ryglová

This paper deals with problems of rural tourism development. The works is focused on researching the situation in the area of rural tourism in the Czech Republic, on understanding the attitudes of entrepreneurial sphere and rural population to this entrepreneurship and mainly on the determination of limiting factors that prevent this form of tourism from a more distinctive development. Rural tourism in the Czech Republic is still in the initiating stage of its development and it is not as developed as in some countries in West Europe. This goal has been reached with the help of primary questionnaire inquiry among business and agricultural subjects in rural areas in the individual regions and the detail results of this questionnaire inquiry are enclosed to the following paper. These identified factors were mainly insufficient financial means and at the same time insufficient state assistance connected with this. Insufficient awareness of this support as well as its inaccessibility for small business appears to be a difficulty, too. From the other obstacles, we can mention the current legislation system, bureaucracy, the state of communication and tourist infrastructure, fears of neighbours’ intolerance and losing privacy and also unfamiliarity and little experience.


2008 ◽  
Vol 52 (No. 6) ◽  
pp. 149-158 ◽  
Author(s):  
M. Šprysl ◽  
J. Čítek ◽  
R. Stupka ◽  
L. Vališ ◽  
M. Vítek

The objective of this study was to document the accuracy of the classification equipment used in the Czech Republic with respect to measurement errors in lean meat percentage prediction such as point of measurement error, equipment error as well as operator error. To this end, a total of 720 pigs were measured in one abattoir. One can say from the results that the correlations between correct and surrogate measurements of fat depth are high (0.95&minus;0.98), for muscle thickness they are lower ranging from 0.49 to 0.88, and for lean meat percentage they are in the range of 0.85&minus;0.95. The lowest correlation (0.49) was calculated for muscle depth measurement between the 2nd and 3rd last rib when the place of measurement was moved 1 cm in the caudal direction, which influenced the level of the FOM correlation. It was further demonstrated that for the second insertion the differences in lean meat percentage prediction ranged from &minus;6.07% to +9.29%. It was also demonstrated that various instruments provided identical measurements of fat depth (<i>r</i> = 0.57&minus;0.97), while for muscle depth the performance was worse (<i>r</i> = 0.38–0.78), which caused a fluctuation in the prediction of lean meat percentage with differences ranging from &minus;2.56% to +2.81%. It can also be concluded that a high agreement between operators was demonstrated for the determination of lean meat percentage (<i>r</i> = 0.71&minus;0.80).


2018 ◽  
Vol 3 (2) ◽  
pp. 90-98
Author(s):  
Damian Czudek

This article focuses on the issue of options, respectively the scope of application of the tax authorities’ discretion in the use of available instruments to ensure the objective of tax administration, i.e. the correct identification and determination of the tax and its payment. The article is based on a case study of the decision of the Supreme Administrative Court of the Czech Republic.


2011 ◽  
Vol 60 (3) ◽  
pp. 239-258
Author(s):  
Jindřiˇka Bojková ◽  
TomᡠSoldán ◽  
Jan ˇpaček ◽  
Michal Straka

Distribution of stoneflies of the family Taeniopterygidae (Plecoptera) in the Czech Republic: earlier data, new records and recent distributional changes Altogether 14 species of Taeniopterygidae were previously mentioned from the Czech Republic; 11 species were listed in check-list of Plecoptera of Czechoslovakia, three species were recorded recently. Main aims of this study were to summarize all known distributional data, reexamine available material, classify species to the categories of threat based on new distributional data, and discuss all species ever supposed to occur in the Czech Republic. In total eight species were found to occur in the Czech Republic with certainty. Two species, Brachyptera seticornis and B. risi, are common in the Czech Republic, the latter exhibiting some expansion in Bohemia at present. Critically endangered Brachyptera braueri missing in the past 40 years has been recently found in several rivers showing some recovery of the original area. Endangered Taeniopteryx nebulosa exhibited considerable decline in lowland and colline streams. New records are presented for near threatened montane species B. starmachi, endangered B. monilicornis, newly classified endangered T. auberti, and vulnerable Taeniopteryx hubaulti. No reliable data have been found as to distribution of Brachyptera trifasciata, Taeniopteryx kuehtreiberi, T. araneoides, and Oemopteryx loewii, respective earlier records were based on misidentification. The occurrence of Rhabdiopteryx acuminata and R. neglecta is doubtful at this moment, because it was based on the problematic determination of larval material. These species cannot be formally considered a part of the Czech fauna due to lack of voucher material. Possible occurrence of other Rhabdiopteryx species showing the area adjacent to the Czech Republic is discussed.


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