Species Identification and Safety of Fish Products

2003 ◽  
Vol 27 ◽  
pp. 481-489 ◽  
Author(s):  
T. Civera
Keyword(s):  
Species Identification ◽  
Fish Products

scholarly journals Evaluation of hygienic quality and labelling of fish distributed in public canteens of Northeast Italy

2016 ◽  
Vol 5 (4) ◽  
Author(s):  
Mariachiara Armani ◽  
Michele Civettini ◽  
Gabriella Conedera ◽  
Michela Favretti ◽  
Dorotea Lombardo ◽  
...  
Keyword(s):  
Species Identification ◽  
Raw Materials ◽  
Food Poisoning ◽  
Microbiological Analysis ◽  
Self Monitoring ◽  
Fish Products ◽  
Health Authorities ◽  
Fish Samples ◽  
Cooking Equipment

Over the past few years, the demand for the introduction of fish products in public canteens (schools, hospitals and nursing-homes) has grown due to their good nutritional proprieties. The particular health conditions and sensitivity of some groups of consumers exposes them to greater risks of food poisoning. It is therefore important to monitor the raw materials that end up in mass catering implementing strategies of mass catering control, both with self-monitoring strategies and with regular controls performed by the competent health authorities. The purpose of this study is to assess the overall quality of seafood dealt out from public catering services located in Northeast Italy. In this paper we illustrate the results of microbiological analysis performed on 135 fish samples (58% of samples were raw fishes, 27% cooked fishes, 6% raw fish products, 9% cooked fish products) and species identification performed on 102 fish samples. Additionally, 135 environmental swabs were collected to determine the effectiveness of cleaning and sanitation of food contact (cutting boards, cooking equipment and food processing surfaces) and non-contact (refrigerator wall and handle, tap lever) surfaces. Of raw seafood samples 24% had total aerobic mesophilic bacteria count &gt;10<sup>5</sup> CFU/g and for <em>Enterobacteriaceae</em> the faecal contamination was excluded since no <em>Salmonella</em> spp. and <em>Escherichia coli</em> were isolated. Just 3.8% of raw seafood samples resulted positive for <em>Listeria monocytogenes</em>. The results of swab samples of cooking utensils and surfaces showed that sanitation practices should be improved. Molecular analysis for fish species identification revealed a mislabelling for 25% of sampled fishes. The results of this survey can provide valuable information for monitoring and surveillance programmes for the control of quality of fish and fish products.

scholarly journals Best BLAST hit alone cannot be used as evidence of fraud

2021 ◽  
Author(s):  
Naiara Rodriguez-Ezpeleta ◽  
Natalia Diaz-Arce
Keyword(s):  
Species Identification ◽  
Dna Markers ◽  
Sequence Database ◽  
Molecular Tools ◽  
Basic Tool ◽  
Fish Products ◽  
Search Tool ◽  
Fish Product

In a recent study, Blanco-Fernandez et al. applied molecular tools to authenticate fish products and conclude evidence of 'worrying international fraud'. They revealed mislabeling in recognizable and unrecognizable fish products labeled as anchovy, hake and tuna commercialized by European companies. Their analyses consisted on extracting DNA from the fish product to be authenticated followed by amplification and sequencing of a suite of DNA markers and on comparing the resulting sequences to the GenBank sequence database using BLAST (Basic Tool Alignment Search Tool) (https://blast.ncbi.nlm.nih.gov/Blast.cgi). By carefully reanalyzing their data, we identify errors in their species identification and conclude that best BLAST hit alone cannot be used as evidence of fraud.

scholarly journals METHODS OF MOLECULAR DIAGNOSTICS FOR FISH SPECIES IDENTIFICATION

Food systems ◽  
2020 ◽  
Vol 3 (3) ◽  
pp. 32-41
Author(s):  
T. A. Fomina ◽  
V. Yu. Kornienko ◽  
M. Yu. Minaev
Keyword(s):  
Species Identification ◽  
Fish Species ◽  
Dna Analysis ◽  
Food Products ◽  
The Body ◽  
Morphological Identification ◽  
Polymorphism Analysis ◽  
Technical Regulation ◽  
Fish Products ◽  
Fish Species Identification

The growth in demand for fish products as a result of globalization of trade caused a risks and threats of selling poor-quality and falsified fish products. This has become a great problem both for supervising agencies and for consumers.Many countries have regulations on food labelling and safety. For example, in the Russian Federation, Republic of Belarus and Republic of Kazakhstan has been passed the Technical Regulation of the Customs Union TR CU022/2011 “Food products in part of their labeling” that aims to prevent misinformation of consumers to ensuring realization of consumer rights to reliable information about food products, and Technical Regulation TR EAEU040/2016 “On safety of fish and fish products” requires indication of the zoological name of the species of the aquatic biological resource or the object of aquaculture.Fish species identification is traditionally carried out based on external morphological traits. However, it becomes impossible to identify species by ichthyological traits upon fish cutting, if the head and fins are removed, and the body is cut on pieces (especially, in case of fillets) and even more so upon technological processing. In this case, objective analytical methods of species identification are used, which are based on ELISA or PCR. However, DNA‑based methods have several advantages compared to ELISA methods and complement traditional morphological identification methods. This paper gives a wide overview of the most recent and used methods of fish species identification based on DNA analysis such as single-strand conformation polymorphism (SSCP) analysis, species-specific PCR, real-time PCR, polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP), DNA barcoding, Sanger sequencing and next-generation sequencing (NGS).

Chemical Species Identification in an SEM by Changes in Emitted X-Ray Energies

1974 ◽  
Vol 32 ◽  
pp. 570-571
Author(s):  
R. H. Duff
Keyword(s):  
Species Identification ◽  
Valence Electron ◽  
Chemical Species ◽  
X Rays ◽  
Chemical Bonds ◽  
Small Shift ◽  
X Ray ◽  
Electron Transitions ◽  
Peak Energy ◽  
Chemical Combination

A material irradiated with electrons emits x-rays having energies characteristic of the elements present. Chemical combination between elements results in a small shift of the peak energies of these characteristic x-rays because chemical bonds between different elements have different energies. The energy differences of the characteristic x-rays resulting from valence electron transitions can be used to identify the chemical species present and to obtain information about the chemical bond itself. Although these peak-energy shifts have been well known for a number of years, their use for chemical-species identification in small volumes of material was not realized until the development of the electron microprobe.

Beetles of the family Cryptophagidae (Coleoptera) in the collection of Zoological Museum of Taras Shevchenko National University of Kyiv

2019 ◽  
Vol 17 (2) ◽  
pp. 10-24 ◽  
Author(s):  
K. Ocheretna
Keyword(s):  
Species Identification ◽  
Broad Sense ◽  
Zoological Museum ◽  
World War ◽  
Identification Number ◽  
Comprehensive List ◽  
Carpathian Region ◽  
The Family ◽  
National University ◽  
Collection Sites

The Cryptophagidae collection (Coleoptera: Cucujoidea) deposited at the Zoological Museum of the Taras Shevchenko National University of Kyiv (ZMKU) is described. The main authors of the collection are well-known researchers from the 1910–1930s, Orest Marcu and Karl Penecke. This is the largest collection of cryptophagids among the natural museums of Ukraine containing 304 specimens belonging to 85 species of 13 genera. In addition, 15 specimens of 5 species belonging to the families Erotylidae, Biphyllidae and Languriidae were among Cryptophagidae specimens. The collection, according to information available in the ZMKU, came to the museum not earlier than 1947 as the indemnity for the results of the II World War, most likely from Chernivtsi, where Marcu and Penecke worked. The vast majority of specimens is collected in the territory of modern Romania and Ukraine, and many specimens came from Chernivtsi. A table with an overview of all key details of the specimens is given, in which there are 6 fields: the name of the species on the label, details on the species identification, number of specimens, collection locality with the name of collector and remarks on the specimen, in particular, the instructions for decoding collection sites from the original labels. Annotations are made on the amount of the collection and the most important specimens and re-identification for each of the 13 genera. Some specimens are lost, probably during numerous collection migrations. In particular, some species (Cryptophagus simplex, C. lapidicola, C. nitidulus, Caenoscelis subdeplanata, Atomaria grandicollis, A. peltata, etc.) are represented in the collection only by the labels. The collection is important for the analysis of the composition of the fauna of the Carpathian region in the broad sense, since some species are encountered in the collection rarely; therefore it is important to clarify their locations to form the most comprehensive list of species of the Cryptophagids in the region. Several species of the family were included on the actual list of the fauna of the region on the basis of the study of this collection, in particular: Atomaria linearis, A. analis, A. apicalis, A. gravidula, Cryptophagus fasciatus, C. setulosus, etc.

Basic principles for assessing the safety of fish products from the ponds exposed to the influence of nuclear fuel cycle enterprises

2019 ◽  
pp. 106-114
Author(s):  
A. V. Trapeznikov ◽  
V. N. Trapeznikova ◽  
A. V. Korzhavin ◽  
V. N. Nikolkin ◽  
A. P. Plataev
Keyword(s):  
Fuel Cycle ◽  
Specific Activity ◽  
River System ◽  
Food Product ◽  
Human Consumption ◽  
Basic Principles ◽  
Fish Products ◽  
Permissible Levels ◽  
Irtysh River

Relevance. In connection with the development of nuclear energy, many aquatic ecosystems have been exposed to radioactive substances. Fish, as an element of biota, is capable to accumulate radionuclides. However, fish is a traditional food. The control of the technogenic radionuclides accumulation level in the ichthyofauna is an important link in ensuring human security.Intention. To analyze the long-term data on the content of long-lived technogenic radionuclides 90Sr and 137Cs in the ichthyofauna of the Ob-Irtysh river system for the period from 2004 to 2016 and Beloyarsky pond for the period from 1977 to 2018.Methodology. Fish as a food product was assessed according to two criteria: a) permissible levels of specific activity of radionuclides (SanPiN 2.3.2.1078-01); b) using the indicator of conformity B and the uncertainty of its definition В (GOST 32161-2013 and GOST 32163-2013).Results and Discussion. Fish of all species that live in the river Tetcha, is not suitable for food use according to the criteria of SanPiN 2.3.2.1078-01, GOST 32161-2013 and GOST 32163-2013. The fish of the Ob-Irtysh river system, the habitat of which is located outside the Tetcha, meets the requirements for fish products. In the period from 1977 to 1989 in the Beloyarsk pond the accumulations of technogenic radionuclides in fish in quantities exceeding sanitary and hygienic standards were possible. Currently, the fish of the Beloyarsky pond fully complies with the sanitary and hygienic requirements for the radiation factor and is safe for human consumption.Conclusion. In the ponds exposed to the atomic energy enterprises, it is necessary to continuously monitor the content of long-lived technogenic radionuclides in fish and assess their amount in accordance with the requirements of SanPiN 2.3.2.1078-01 and using the conformity indicator В and the uncertainty of its determination В. 

Species identification of raw and cooked bivalves using electrophoresis

2000 ◽  
Vol 20 (3) ◽  
pp. 367-377 ◽  
Author(s):  
Monique Etienne ◽  
Marc Jérôme ◽  
Joël Fleurence
Keyword(s):  
Species Identification
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