scholarly journals Dimethyl disulfide exerts antifungal activity against Sclerotinia minor by damaging its membrane and induces systemic resistance in host plants

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Swati Tyagi ◽  
Kui-Jae Lee ◽  
Pratyoosh Shukla ◽  
Jong-Chan Chae
Keyword(s):  
Pathogenic Fungus ◽  
Dimethyl Disulfide ◽  
Systemic Resistance ◽  
Ultrastructural Changes ◽  
Ergosterol Biosynthesis ◽  
Sclerotinia Minor ◽  
Tomato Plants ◽  
Expression Of Genes ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting

Abstract Microbial volatile compounds (MVCs) significantly influence the growth of plants and phytopathogens. However, the practical application of MVCs at the field level is limited by the fact that the concentrations at which these compounds antagonize the pathogens are often toxic for the plants. In this study, we investigated the effect of dimethyl disulfide (DMDS), one of the MVCs produced by microorganisms, on the fitness of tomato plants and its fungicidal potential against a fungal phytopathogen, Sclerotinia minor. DMDS showed strong fungicidal and plant growth promoting activities with regard to the inhibition of mycelial growth, sclerotia formation, and germination, and reduction of disease symptoms in tomato plants infected with S. minor. DMDS exposure significantly upregulated the expression of genes related to growth and defense against the pathogen in tomato. Especially, the overexpression of PR1 and PR5 suggested the involvement of the salicylic acid pathway in the induction of systemic resistance. Several morphological and ultrastructural changes were observed in the cell membrane of S. minor and the expression of ergosterol biosynthesis gene was significantly downregulated, suggesting that DMDS damaged the membrane, thereby affecting the growth and pathogenicity of the fungus. In conclusion, the tripartite interaction studies among pathogenic fungus, DMDS, and tomato revealed that DMDS played roles in antagonizing pathogen as well as improving the growth and disease resistance of tomato. Our findings provide new insights into the potential of volatile DMDS as an effective tool against sclerotial rot disease.

scholarly journals Induction of Bacterial Canker Resistance in Tomato Plants Using Plant Growth Promoting Rhizobacteria

2019 ◽  
Vol 13 (1) ◽  
pp. 215-222 ◽  
Author(s):  
Yuliya Kolomiiets ◽  
Ivan Grygoryuk ◽  
Artur Likhanov ◽  
Lyudmila Butsenko ◽  
Yaroslav Blume
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Cell Suspensions ◽  
Systemic Resistance ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Canker ◽  
Tomato Plants ◽  
High Productivity ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background: By inducing the production of inhibitory allelochemicals and mechanisms of systemic resistance plant growth promoting bacteria (PGPB) help plants to cope with stresses. Materials and Methods: In this study cell suspensions of Bacillus subtilis, Pseudomonas fluorescens or Azotobacter chroococcum were used to test the efficacy of these PGPB in inducing resistance in tomato (Lycopersicon esculentum Mill) against Clavibacter michiganensis subsp michiganensis, a bacteria known to cause canker disease. To test this hypothesis, seedlings of Chaika variety, characterized by short growing, early-ripening, high productivity and resistance against fusarium and the C. michiganensis strain ІZ-38 isolated in Kyiv were employed. Results and Conclusion: The use of cell suspensions of the PGPB B. subtilis, A. chroococcum or P. fluorescens induced an increment in the resistance of tomato plants against the causative agent of bacterial canker (C. michiganensis subsp. michiganensis) by 42–50%. PGPB in fact promoted in C. michiganensis infected tomato plants: i) the accumulation of chlorophyll a and b and carotenoids; ii) the thickening of the upper and lower epidermis of leaves; iii) the deposition of biopolymers with protective properties in epidermal cells; iv) the activity of the peroxidase enzyme and v) the net productivity of photosynthesis.

scholarly journals Author Correction: Dimethyl disulfide exerts antifungal activity against Sclerotinia minor by damaging its membrane and induces systemic resistance in host plants

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Swati Tyagi ◽  
Kui-Jae Lee ◽  
Pratyoosh Shukla ◽  
Jong-Chan Chae
Keyword(s):  
Antifungal Activity ◽  
Host Plants ◽  
Dimethyl Disulfide ◽  
Systemic Resistance ◽  
Sclerotinia Minor

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Tissue-specific diversity of bacterial endophytes in Mexican husk tomato plants (Physalis ixocarpa Brot. ex Horm.), and screening for their multiple plant growth-promoting activities

2021 ◽  
pp. 100028
Author(s):  
Claudia E. Hernández-Pacheco ◽  
Ma del Carmen Orozco-Mosqueda ◽  
Aurora Flores ◽  
Eduardo Valencia-Cantero ◽  
Gustavo Santoyo
Keyword(s):  
Plant Growth ◽  
Bacterial Endophytes ◽  
Tomato Plants ◽  
Tissue Specific ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Husk Tomato

scholarly journals Intercorrelation mechanism of Hypoxia and Cordycepin Biosynthesis in Zombie fungus

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Ahmad Suparmin ◽  
Tatsuya Kato ◽  
Hiroyuki Takemoto ◽  
Enoch Y. Park
Keyword(s):  
Aminolevulinic Acid ◽  
Iron Acquisition ◽  
Pathogenic Fungus ◽  
Gas Chromatography Mass Spectrometry ◽  
Ergosterol Biosynthesis ◽  
Hypoxic Conditions ◽  
Expression Of Genes ◽  
Hypoxic Environment ◽  
Oxidative Phosphorylation Pathway ◽  
Coproporphyrinogen Iii Oxidase

Cordycepin is an anticancer metabolite produces by a zombie fungus species of Cordyceps militaris. They are capable to infect and hijack insect’s nervous neuron system. Hypoxic environment commonly must be faced by the pathogenic fungus during infection either this zombie fungus. They activate oxygen sensing mode, heme, siderophore, and sterol biosynthesis to overcome it. Underlined our previous study that liquid surfaced culture of C. militaris NBRC103752 produced a higher amount of cordycepin than submerged culture, suggesting that hypoxic conditions might induce it. However, when and how the mechanism of cordycepin production started in liquid surfaced culture is not understood, yet. In our present study, the combination of transcriptomics and gas chromatography-mass spectrometry were carried out during the production phases of cordycepin (5d, 12d, and 19d of incubation periods) and the mechanism of cordycepin production was figured out. The expression of genes in the fermentation pathway and the oxidative phosphorylation pathway were significantly upregulated and down regulated, respectively. Expression of four genes in the heme biosynthesis, including 5-aminolevulinic acid synthase (CCM_01504), delta-aminolevulinic acid dehydratase (CCM_00935), coproporphyrinogen III oxidase (CCM_07483) and cytochrome c oxidase15 (CCM_05057) were upregulated at the beginning of the exponential phase (12d). Further, the activation of Zn(2)-C6 transcription factor that regulates the iron acquisition and ergosterol biosynthesis significantly upregulated and a metabolite reporter adenosine was detected only at 12d. The results in the present study show the correlation between hypoxia and the accumulation of heme before cordycepin biosynthesis.

scholarly journals Seed Biopriming– A Review

2020 ◽  
Vol 7 (1) ◽  
pp. 038-043
Author(s):  
Amol J. Deshmukh ◽  
◽  
R. S. Jaiman ◽  
R. P. Bambharolia ◽  
Vijay A. Patil ◽  
...  
Keyword(s):  
Trichoderma Viride ◽  
Seed Priming ◽  
Systemic Resistance ◽  
Solid Matrix ◽  
Seedling Vigour ◽  
Vesicular Arbuscular ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Almost All ◽  
Seed Biopriming

There are various seed priming methods such as hydro priming, osmo priming, solid matrix priming, hormo-priming, chemo-priming, nutri-priming and biopriming being useful for enhancing the seed germination, seedling vigour and to overcome abiotic stress. Moreover to these advantages, of all priming methods only biopriming method gives an extra advantage of biotic stress management and thus gain special attention. Solubilisation or mobilization of soil macro and micronutrients, siderophore production, induction of plant growth promoting activities, induction of useful biochemicals, phytoalexin and defense-related enzymes and induced systemic resistance are the mechanisms involved in seed biopriming. Range of fungal or bacterial bio agents viz., Azotobacter, Rhizobium, Arthrobacter, Agrobacterium, Azospirillum, Enterobacter, Streptomyces, Bacillus, Burkholderia, Klebsiella, PSB, Pseudomonas fluorescence, Trichoderma viride, Trichoderma harzianum and Vesicular Arbuscular Mycorhiza, whether they are biofertilizer or biopesticide, may be useful as biopriming agents. Seed biopriming is useful in almost all the crops over the globe and is an eco-friendly substitute to chemical fungicides.

Diversity and plant growth promoting activities of the cultivable rhizo-bacteria of Dongxiang wild rice (Oryza rufipogon)

2011 ◽  
Vol 19 (4) ◽  
pp. 476-484 ◽  
Author(s):  
Luo Fei ◽  
Wang Ya ◽  
Zeng Qinggui ◽  
Yan Riming ◽  
Zhang Zhibin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Wild Rice ◽  
Oryza Rufipogon ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Dongxiang Wild Rice

Interaction of Tomatoes (Solanum lycopersicum L.) Transformed by rapA1 Gene with Pseudomonas sp. 102 Bacteria Resistant to High Cadmium Concentrations as a Basis for Effective Symbiotic Phytoremediation System

2019 ◽  
pp. 38-48
Author(s):  
L.R. Khakimova ◽  
A.M. Lavina ◽  
L.R. Karimova ◽  
V.V. Fedyaev ◽  
An.Kh. Baymiev ◽  
...  
Keyword(s):  
Growth Parameters ◽  
Basic Research ◽  
Toxic Effects ◽  
Research Centre ◽  
Pseudomonas Sp ◽  
Tomato Plants ◽  
Solanum Lycopersicum L ◽  
Plant Growth Promoting ◽  
High Concentrations ◽  
The Government

A Pseudomonas sp. 102 strain, which is highly resistant to toxic effects of cadmium and has plant growth-promoting activity, can significantly increase growth parameters and biomass of tomato plants, including those observed under toxic effects of cadmium. The greatest positive effect was observed in plants transformed with the bacterial adhesin gene rapA1, the product of which is important for colonization of plant roots by bacteria. It was also shown that shoots of transgenic tomato plants accumulated the greatest amount of cadmium during inoculation with Pseudomonas sp. 102. The ability to extract high concentrations of cadmium and accumulate a large biomass under stress opens up prospects for the further use of associative interactions between tomato and Pseudomonas for phytoremediation. phytoremediation, cadmium, tomato, Pseudomonas, inoculation, agglutinins, This study was carried out using the equipment of the Biomika Centre for Collective Use of the Institute of Biochemistry and Genetics (Ufa Federal Research Centre, Russian Academy of Sciences) as part of the government task (project no. AAAA-A16-1160203500284). This study was supported by the Russian Foundation for Basic Research (project nos. 18-34-20004 and 18-34-00033) and 18-344-0033 mol_a_ved and 34-00033 mol_a).

scholarly journals C4 Bacterial Volatiles Improve Plant Health

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 682
Author(s):  
Bruno Henrique Silva Dias ◽  
Sung-Hee Jung ◽  
Juliana Velasco de Castro Oliveira ◽  
Choong-Min Ryu
Keyword(s):  
Plant Growth ◽  
Volatile Compounds ◽  
Large Scale ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Health ◽  
Systemic Resistance ◽  
Potential Applications ◽  
Plant Growth Promoting ◽  
Bacterial Volatiles

Plant growth-promoting rhizobacteria (PGPR) associated with plant roots can trigger plant growth promotion and induced systemic resistance. Several bacterial determinants including cell-wall components and secreted compounds have been identified to date. Here, we review a group of low-molecular-weight volatile compounds released by PGPR, which improve plant health, mostly by protecting plants against pathogen attack under greenhouse and field conditions. We particularly focus on C4 bacterial volatile compounds (BVCs), such as 2,3-butanediol and acetoin, which have been shown to activate the plant immune response and to promote plant growth at the molecular level as well as in large-scale field applications. We also disc/ uss the potential applications, metabolic engineering, and large-scale fermentation of C4 BVCs. The C4 bacterial volatiles act as airborne signals and therefore represent a new type of biocontrol agent. Further advances in the encapsulation procedure, together with the development of standards and guidelines, will promote the application of C4 volatiles in the field.

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