scholarly journals Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Raluca M. Boteanu ◽  
Viorel I. Suica ◽  
Luminita Ivan ◽  
Florentina Safciuc ◽  
Elena Uyy ◽  
...  
Keyword(s):  
Bone Healing ◽  
Cultured Cells ◽  
Traumatic Event ◽  
Healing Process ◽  
Dip Coating ◽  
Polymer Coatings ◽  
Titanium Implants ◽  
Mass Spectrometry Analysis ◽  
Defect Model ◽  
Tibial Defect

Abstract Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing becomes a clinical problem and affects the patient's quality of life. We aimed to design a new bioactive surface of titanium implants with a synergetic PEG biopolymer-based composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4) during bone healing. The optimal architecture of non-cytotoxic polymeric coatings deposited by dip coating under controlled parameters was assessed both in cultured cells and in a rat tibial defect model (100% viability). Notably, the titanium adsorbed polymer matrix induced an improved healing process when compared with the individual action of each biomolecules. High-performance mass spectrometry analysis demonstrated that recovery after a traumatic event is governed by specific differentially regulated proteins, acting in a coordinated response to the external stimulus. Predicted protein interactions shown by STRING analysis were well organized in hub-based networks related with response to chemical, wound healing and response to stress pathways. The proposed functional polymer coatings of the titanium implants demonstrated the significant improvement of bone healing process after injury.

Development of a Segmental Long-bone Defect Model in Sheep

1998 ◽  
Vol 11 (01) ◽  
pp. 01-07 ◽  
Author(s):  
P. Frayssinet ◽  
E. Asimus ◽  
G. Chanoit ◽  
P. Collard ◽  
A. Autefage ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Healing ◽  
Healing Process ◽  
Biological Properties ◽  
Long Bone ◽  
Defect Model ◽  
Metatarsal Bones ◽  
Group 2 ◽  
Long Bone Defect ◽  
Group 1

SummaryA 10 mm-long (Group #1) or 20 mmlong (Group #2) segmental osteoperiosteal defect was performed on the metatarsus of ten adult ewes (5+5). The goal of the study was to search for a critical size defect model leading to nonunion. The bone gap was maintained for three months with an internal fixation device involving two plates set in orthogonal planes. Radiological and histological examinations were performed on harvested metatarsal bones. Three months after surgery Group #1 animals showed obvious signs of bone healing without achieving complete union in all cases. Evidence of a healing process was not observed in Group #2 animals, and histological examination confirmed the complete failure of bone repair in the 20 mm gaps. These results are comparable to those of other authors who have concluded that a bone gap corresponding to 1.4 times the diaphyseal diameter overshoots physiological bone healing capacities. This long bone defect model showed good biological properties allowing callus settlement with minimal impairment in Group #1 and permitted weightbearing and unrestricted motion in the animals. Such a sheep model would be useful for testing hard tissue biomaterials, bone healing enhancement or further developed as an experimental nonunion model.Metatarsal diaphyseal defects (length: 10 or 20 mm) maintained with plates were performed in sheep in search of nonunion after a three-month period. Radiological and histological examinations showed that 10 mm gaps healed spontaneously while 20 mm gaps did not. These results are comparable to those of other authors who concluded that a diaphyseal defect whose length exceeds 1.4 times its diameter is unable to repair. The good biological properties exhibited by this defect model seem to be convenient for testing bone substitutes or bone healing enhancement techniques.

Evaluation of the bone healing process in an experimental tibial bone defect model in ovariectomized rats

2014 ◽  
Vol 26 (5) ◽  
pp. 473-481 ◽  
Author(s):  
Hueliton Wilian Kido ◽  
Paulo Sérgio Bossini ◽  
Carla Roberta Tim ◽  
Nivaldo Antônio Parizotto ◽  
Anderson Ferreira da Cunha ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Healing ◽  
Healing Process ◽  
Ovariectomized Rats ◽  
Defect Model ◽  
Tibial Bone ◽  
Bone Healing Process

Osteomacs are critical for optimal intramembranous bone formation in a tibial defect model of bone healing

Bone ◽  
2009 ◽  
Vol 44 ◽  
pp. S30
Author(s):  
K.A. Alexander ◽  
L.J. Raggatt ◽  
M.K. Chang ◽  
E.R. Maylin ◽  
R. Muller ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Healing ◽  
Defect Model ◽  
Tibial Defect ◽  
Intramembranous Bone ◽  
Intramembranous Bone Formation

scholarly journals In vivo efficacy and toxicity of synthesized nano-β-tricalcium phosphate in a rabbit tibial defect model

2018 ◽  
Vol 2 ◽  
pp. 239784731881949
Author(s):  
Jer Ping Ooi ◽  
Shah Rizal Kasim ◽  
Rumaizi Bin Shaari ◽  
Nor Aini Saidin
Keyword(s):  
Inflammatory Response ◽  
Tricalcium Phosphate ◽  
Histopathological Examination ◽  
Healing Process ◽  
Control Group ◽  
Precipitation Process ◽  
Defect Model ◽  
Defect Site ◽  
Tibial Defect

Previous studies of the biocompatibility of β-tricalcium phosphate (β-TCP) focused on bulk-sized β-TCP, and little is known about the biocompatibility of nano β-TCP particles (nβ-TCP). The objectives of this study were to synthesize nβ-TCP particles and determine their efficacy in a rabbit tibial defect model. The nβ-TCP particles were first synthesized using a wet chemical precipitation process. The particles were then implanted in the left tibia of New Zealand white rabbits, and the defect site healing was evaluated for a period of 16 weeks using radiography, computed tomography, and histology. Data were compared with those of a sham (empty) control. Results showed that the defect site treated with nβ-TCP particles did not heal completely after 16 weeks, whereas full cortical bone recovery was observed in the sham control group of rabbits. Histopathological examination showed that the nβ-TCP particles caused an excessive and prolonged inflammatory response by the host. The nano-scaled size and biodegradability of the synthesized nβ-TCP particles may have been responsible for this progressive and extended inflammatory response, which delayed the bone healing process. The underlying mechanism for this effect remains unclear and warrants further investigation.

scholarly journals Can Optimizing the Mechanical Environment Deliver a Clinically Significant Reduction in Fracture Healing Time?

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 691
Author(s):  
Jan Barcik ◽  
Devakara R. Epari
Keyword(s):  
Fracture Healing ◽  
Bone Healing ◽  
Mechanical Stimulation ◽  
Callus Formation ◽  
Weight Bearing ◽  
Healing Process ◽  
Healing Time ◽  
Mechanical Environment ◽  
Clinically Significant ◽  
The Impact

The impact of the local mechanical environment in the fracture gap on the bone healing process has been extensively investigated. Whilst it is widely accepted that mechanical stimulation is integral to callus formation and secondary bone healing, treatment strategies that aim to harness that potential are rare. In fact, the current clinical practice with an initially partial or non-weight-bearing approach appears to contradict the findings from animal experiments that early mechanical stimulation is critical. Therefore, we posed the question as to whether optimizing the mechanical environment over the course of healing can deliver a clinically significant reduction in fracture healing time. In reviewing the evidence from pre-clinical studies that investigate the influence of mechanics on bone healing, we formulate a hypothesis for the stimulation protocol which has the potential to shorten healing time. The protocol involves confining stimulation predominantly to the proliferative phase of healing and including adequate rest periods between applications of stimulation.

scholarly journals Influence of the Thermal Treatment to Address a Better Osseointegration of Ti6Al4V Dental Implants: Histological and Histomorphometrical Study in a Rabbit Model

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Antonio Scarano ◽  
Ezio Crocetta ◽  
Alessandro Quaranta ◽  
Felice Lorusso
Keyword(s):  
Thermal Treatment ◽  
Dental Implants ◽  
Bone Healing ◽  
Rabbit Model ◽  
Titanium Implants ◽  
Control Group ◽  
Left Femur ◽  
Bone Response ◽  
First Choice ◽  
Significant Difference

Background. Pure titanium continues to be the first choice for dental implants and represents the gold standard for their biocompatibility and physical and mechanical characteristics, while the titanium alloy (Ti6Al4V) has good mechanical properties. The surface structure of the titanium oxide layer formation on the surface influences and improves the bone response around dental implants. Purpose. The purpose of this study is to evaluate the influence of a thermal treatment of Ti6Al4V implant surfaces and the bone healing response in a rabbit model. Methods. Altogether sixteen implants with same design were inserted into the distal femoral metaphysis. A screw (13 mm long, 4 mm in diameter) was inserted in an implant bed. Each rabbit received two implants, one in the left femur and one in the right femur. The samples were histologically and histomorphometrically evaluated at 8 weeks. Results. A statistically significant difference (p = 0.000034) was present histologically in the percentages of bone-implant contact (BIC) between the test group (BIC = 69.25±4.49%.) and control group (BIC = 56.25 ± 4.8%) by one-way analysis of variance (ANOVA). Significance was set at p ≤ 0.05. Conclusions. The outcome of the present study indicates a novel approach to improving bone healing around titanium implants.

Thyroid Hormones May Influence Cortical Bone Healing Around Titanium Implants: A Histometric Study in Rats

2008 ◽  
Vol 79 (5) ◽  
pp. 881-887 ◽  
Author(s):  
Daniela da Silva Feitosa ◽  
Beatriz de Brito Bezerra ◽  
Gláucia Maria Bovi Ambrosano ◽  
Francisco Humberto Nociti ◽  
Márcio Zaffalon Casati ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Cortical Bone ◽  
Bone Healing ◽  
Titanium Implants

scholarly journals Bone Regeneration Assessment of Polycaprolactone Membrane on Critical-Size Defects in Rat Calvaria

Membranes ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 124
Author(s):  
Ana Paula Farnezi Bassi ◽  
Vinícius Ferreira Bizelli ◽  
Tamires Mello Francatti ◽  
Ana Carulina Rezende de Moares Ferreira ◽  
Járede Carvalho Pereira ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Healing ◽  
Inflammatory Responses ◽  
Blood Clot ◽  
Healing Process ◽  
Male Rats ◽  
Control Group ◽  
Collagen Membrane ◽  
Large Area ◽  
Rat Calvaria

Biomaterials for use in guided bone regeneration (GBR) are constantly being investigated and developed to improve clinical outcomes. The present study aimed to comparatively evaluate the biological performance of different membranes during the bone healing process of 8 mm critical defects in rat calvaria in order to assess their influence on the quality of the newly formed bone. Seventy-two adult male rats were divided into three experimental groups (n = 24) based on the membranes used: the CG—membrane-free control group (only blood clot, negative control), BG—porcine collagen membrane group (Bio-Guide®, positive control), and the PCL—polycaprolactone (enriched with 5% hydroxyapatite) membrane group (experimental group). Histological and histometric analyses were performed at 7, 15, 30, and 60 days postoperatively. The quantitative data were analyzed by two-way ANOVA and Tukey’s test (p < 0.05). At 7 and 15 days, the inflammatory responses in the BG and PCL groups were significantly different (p < 0.05). The PCL group, at 15 days, showed a large area of newly formed bone. At 30 and 60 days postoperatively, the PCL and BG groups exhibited similar bone healing, including some specimens showing complete closure of the critical defect (p = 0.799). Thus, the PCL membrane was biocompatible, and has the potential to help with GBR procedures.

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