scholarly journals Cut-off value of D. pteronyssinus specific IgE in double negative patients Der p 1 and Der p 2 and its clinical repercussion

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Antonio Letrán ◽  
Ignacio García ◽  
Marisa Espinazo-Romeu ◽  
Carmen Moreno-Aguilar ◽  
Francisco Moreno
Keyword(s):  
Roc Curves ◽  
Specific Ige ◽  
Diagnostic Techniques ◽  
Cross Sectional Study ◽  
Double Negative ◽  
Double Positive ◽  
Cross Sectional ◽  
Prick Test ◽  
Der P 1 ◽  
Der P 2

AbstractAccessibility to more precise diagnostic techniques such as component resolved diagnostics (CRD), provides us with an important advance in diagnostic aspects as well as treatment. The subject of this study aims to better understand the profiles of sensitization to Der p 1, Der p 2 and Der p 23 and to know to what extent their use could help us in optimizing the decision-making for their treatment with Specific Immunotherapy. Cross-sectional study of subjects older than 5 years, diagnosed with allergy to HDM using skin prick test and sIgE, with symptoms of rhinitis and/or asthma. Total and specific IgE was determined to D. pteronyssinus, nDer p 1, rDer p 2 and rDer p 23 using ImmunoCAP. 240 patients were recruited (97.1% rhinitis and 46.25% rhinitis and asthma). Four different phenotypes were observed: positive or negative for sIgE nDer p 1 and/or IgE rDer p 2. 17% of these patients sIgE were double negative for Der p 1 and Der p 2 (increasing with age and with significantly lower sIgE levels than the rest of the groups). Using ROC curves, value less than 2.18 KUA/L for D. pteronyssinus sIgE gave us a sensitivity and specificity of 0.882 and 0.985, respectively, to double negative IgE nDer p 1 and IgE rDer p 2 group. Despite positive SPT and sIgE to D. pteronyssinus, 17% of the studied population is IgE nDer p 1 and IgE rDer p 2 double negative, with a cut-off value of 2.18 KU/L, which is very relevant for taking of decisions in prescription of AIT. The double positive population sIgE nDer p 1 and IgE rDer p 2 is associated with asthma compared to the other groups and this does not seem to be influenced by IgE rDer p 23.

scholarly journals “Cut-Off Value of Sige To D. Pteronyssinus in Double Negative Patients Der P 1 and Der P2 and Its Clinical Repercussion”.

2021 ◽  
Author(s):  
Antonio Letrán ◽  
Ignacio García ◽  
Marisa Espinazo-Romeu ◽  
Carmen Moreno-Aguilar ◽  
Francisco Moreno
Keyword(s):  
Roc Curves ◽  
Specific Ige ◽  
Diagnostic Techniques ◽  
Cross Sectional Study ◽  
Double Negative ◽  
Double Positive ◽  
Cross Sectional ◽  
Prick Test ◽  
Der P 1 ◽  
Der P 2

Abstract Background Accessibility to more precise diagnostic techniques such as component resolved diagnostics (CRD), provides us with an important advance in diagnostic aspects as well as treatment. Objective The subject of this study aims to better understand the profiles of sensitization to Der p 1, Der p 2 and Der p 23 and to know to what extent their use could help us in optimizing the decision-making for their treatment with Specific Immunotherapy. Method Cross-sectional study of subjects older than 5 years, diagnosed with allergy to HDM using skin prick test and sIgE, with symptoms of rhinitis and/or asthma. Total and specific IgE was determined to D. pteronyssinus, nDer p 1, rDer p 2 and rDer p 23 using ImmunoCAP. Results 240 patients were recruited (97.1% rhinitis and 46.25% rhinitis and asthma). Four different phenotypes were observed: positive or negative for sIgE nDer p 1 and/or IgE rDer p 2. 17% of these patients sIgE were double negative for Der p 1 and Der p 2 (increasing with age and with significantly lower sIgE levels than the rest of the groups). Using ROC curves, value less than 2.18 KU A /L for D. pteronyssinus sIgE gave us a sensitivity and specificity of 0.882 and 0.985, respectively, to double negative IgE nDer p 1 and IgE rDer p 2 group. Conclusions Despite positive SPT and sIgE to D. pteronyssinus, 17% of the studied population is IgE nDer p 1 and IgE rDer p 2 double negative, with a cut-off value of 2.18 KU/ L, which is very relevant for taking of decisions in prescription of AIT. The double positive population sIgE nDer p 1 and IgE rDer p 2 is associated with asthma compared to the other groups and this does not seem to be influenced by IgE rDer p 23.

The Distinguishing Clinical Features of Nonallergic Rhinitis Patients

2019 ◽  
Vol 33 (5) ◽  
pp. 524-530 ◽  
Author(s):  
Aneeza W. Hamizan ◽  
Mark Azer ◽  
Raquel Alvarado ◽  
Peter Earls ◽  
Henry P. Barham ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Clinical Characteristics ◽  
Immunoglobulin E ◽  
Inferior Turbinate ◽  
Specific Ige ◽  
Cross Sectional Study ◽  
Nonallergic Rhinitis ◽  
Cross Sectional ◽  
Prick Test ◽  
Inhalant Allergy

Background Not all rhinitis patients are affected by an immunoglobulin E (IgE)-mediated inflammatory process. Skin and serum allergy assessments are limited in their ability to define local allergic rhinitis (LAR). Thus, patients with negative systemic allergy assessments comprise a mix of those who truly have nonallergic rhinitis (NAR) and patients with LAR. Objective To determine the clinical characteristics of patients with NAR. Methods A cross-sectional study was performed on consecutive adults with rhinitis symptoms who underwent turbinate surgery. NAR patients were defined by excluding allergy using both systemic (serum-specific IgE and/or skin prick test) and local (inferior turbinate tissue-specific IgE) tests. Allergic rhinitis (AR) patients were defined by any positive systemic or local test toward aeroallergens. The clinical characteristics studied included allergic comorbidities (asthma, eczema, allergic conjunctivitis), inhalant allergen triggers (dust, pollen, animal dander), and environmental triggers (Cincinnati Irritant Index [CII]). Results There were 154 participants (41.79 ± 14.78 years, 37.7% female). NAR patients (11.7%) were older (49.33 ± 15.99 vs 40.78 ± 14.38 years, P = .02), had less self-reported asthma (5.6% vs 36.3%, P < .01) and house dust inhalant trigger (38.9 vs 65.2%, P = .03) compared to AR patients. The CII score was similar for NAR and AR (31.06 ± 28.88 vs 35.49 ± 24.70, P = .61). Conclusion Patients who were older, without asthma, and lacked an inhalant allergy trigger were more likely to have true NAR. Environmental triggers are not distinguishing features of NAR. This may be used as a guide to identify rhinitis patients whose symptoms are truly nonallergic etiology compared to those with falsely negative systemic allergy assessment but may still need management for LAR.

scholarly journals Skin prick test reactivity in atopic children and their number of siblings

2015 ◽  
Vol 55 (4) ◽  
pp. 189
Author(s):  
Beatrix Siregar ◽  
Lily Irsa ◽  
Supriatmo Supriatmo ◽  
Sjabaroeddin Loebis ◽  
Rita Evalina
Keyword(s):  
Skin Prick Test ◽  
Specific Ige ◽  
Cross Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Positive Skin ◽  
Prick Test ◽  
Group I ◽  
History Of ◽  
Skin Prick Tests

Background Some studies have shown that low birth order is a risk factor for developing atopy, although these results remain inconclusive. Those studies put forth the hygiene hypothesis, which states that early childhood infections in siblings may protect against atopy. Hence, an inverse relationship between family numbers and atopy was found. Atopy may be diagnosed from a history of atopy in an individual or his family, and can be confirmed by specific IgE for allergens or positive skin prick tests.Objective To assess for an association between skin prick test reactivity in atopic children and their number of siblings.Methods A cross-sectional study was conducted in May to June 2010 in elementary school children at the Kampung Baru District, Medan Regency, North Sumatera. Subjects were divided into two groups. Group I had children with < 3 siblings and group II had children with ≥ 3 siblings. Skin prick tests were done in 7 to 10-year-old children with a history of asthma, allergic rhinitis and atopic dermatitis. Skin prick test reactivity results were analyzed by Chi-square test.Results A total of 192 subjects were enrolled in this study, with 96 subjects in each group. Positive skin prick tests were significantly higher in subjects with <3 siblings than in those with >3 siblings (75% and 53.1%, respectively; P=0.003).Conclusion Atopic children with <3 siblings had more positive skin prick tests than children with >3 siblings.

Baked Tolerance in Cow’s Milk Allergy: Quite Frequent, Hard to Predict!

2020 ◽  
pp. 1-5
Author(s):  
Lisis Karine Vilar ◽  
Flávia Alves Araújo ◽  
Thalita Pereira Santos ◽  
Thatiana Tavares Menezes ◽  
Marina F. Cheik ◽  
...  
Keyword(s):  
Food Challenge ◽  
Specific Ige ◽  
Cross Sectional Study ◽  
Cow’S Milk ◽  
Cross Sectional ◽  
Prick Test ◽  
Cow's Milk ◽  
Baked Goods ◽  
Baked Foods ◽  
Baked Milk

<b><i>Introduction:</i></b> Cow’s milk protein allergy (CMA) is the most common type of food allergy in childhood and exclusion diet is a challenge for patients. <b><i>Objective:</i></b> The study aim was to investigate the frequency of tolerance to baked foods containing milk and evaluate immediate skin prick test (SPT) and specific IgEs for different cow’s milk (CM) protein types as predictors of tolerance to baked foods containing milk for CMA patients. <b><i>Methods:</i></b> A cross-sectional study was performed. Fifty-four CMA patients were enrolled and oral food challenge (OFC) was performed with baked product, 6 different milk SPTs and specific IgEs to CM, casein, α-lactalbumin, and β-lactoglobulin. <b><i>Results:</i></b> Thirty-nine (72.2%) patients tolerated OFC with baked milk cupcake. CM-specific IgE and casein SPT showed statistical difference between positive and negative OFC groups. Probability curves for baked milk tolerance were created for specific CM IgE (<i>Z</i> = 2.542, <i>p</i> &#x3c; 0.0110) and casein SPT (<i>Z</i> = 2.290, <i>p</i> &#x3c; 0.0220) using logistic regression. <b><i>Conclusions:</i></b> The high percentage of patients able to tolerate baked goods enables an improvement in intake possibilities and quality of life of CMA patients and families. Specific CM IgE and casein SPT demonstrated to be useful predictors in relation to baked milk tolerance.

scholarly journals Skin prick test is more useful than specific IgE for diagnosis of buckwheat allergy: A retrospective cross-sectional study

2018 ◽  
Vol 67 (1) ◽  
pp. 67-71 ◽  
Author(s):  
Noriyuki Yanagida ◽  
Sakura Sato ◽  
Kyohei Takahashi ◽  
Ken-ichi Nagakura ◽  
Kiyotake Ogura ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
Specific Ige ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Prick Test

scholarly journals Prevalence and Risk Factors of Allergic Bronchopulmonary Aspergillosis and Aspergillus Sensitization in Children with Poorly Controlled Asthma

2019 ◽  
Vol 66 (3) ◽  
pp. 275-283
Author(s):  
Jyoti Kumari ◽  
Kana Ram Jat ◽  
Rakesh Lodha ◽  
Manisha Jana ◽  
Immaculata Xess ◽  
...  
Keyword(s):  
Risk Factors ◽  
Immunoglobulin E ◽  
Care Center ◽  
Tertiary Care ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Specific Ige ◽  
Cross Sectional Study ◽  
High Resolution Computed Tomography ◽  
Cross Sectional ◽  
Prick Test

Abstract Background Allergic bronchopulmonary aspergillosis (ABPA) may be a risk factor for poorly controlled asthma in children. The studies regarding prevalence and risk factors of ABPA in children with poorly controlled asthma are limited in number. Objectives To determine prevalence and risk factors of ABPA and aspergillus sensitization (AS) in children with poorly controlled asthma. Methods In this prospective cross-sectional study from a tertiary care center in India, we enrolled asthmatic children 5–15 years of age with poorly controlled asthma. We did the following investigations: spirometry, skin prick test, serum total immunoglobulin E (IgE), aspergillus-specific IgE and immunoglobulin G, serum precipitin for Aspergillus, absolute eosinophil count, chest X-ray and high-resolution computed tomography of the chest. ABPA and AS were diagnosed as per the recently proposed criteria. Results We enrolled 106 children [boys 72 (67.9%); mean age of 10.2 ± 2.6 years] with poorly controlled asthma. The prevalence of ABPA and AS were 11.3% (95% CI, 5.2–17.5%) and 61.3% (95% CI, 52.0–70.7%), respectively. The presence of brownish sputum was significantly more in ABPA compared with non-ABPA patients (33.3 vs. 4.2%, p = 0.002). The age, gender, allergic rhinitis and gastroesophageal reflux were not significantly different in ABPA compared with non-ABPA patients. Conclusion The prevalence of ABPA and AS was 11.3 and 61.3%, respectively in children with poorly controlled asthma. We could not find any risk factors for ABPA except that the presence of brownish sputum was more in children with ABPA. Spirometry parameters were not significantly different in ABPA compared with non-ABPA patients.

scholarly journals Serum specific IgE responses to inhalant allergens sensitization

2017 ◽  
Vol 26 (3) ◽  
pp. 224-8
Author(s):  
Iris Rengganis ◽  
Suriani Alimuddin ◽  
Agus J. Susanto
Keyword(s):  
Immunoglobulin E ◽  
Specific Ige ◽  
Cross Sectional Study ◽  
Positive Skin Prick Test ◽  
Dust Mites ◽  
Storage Mites ◽  
Cross Sectional ◽  
Positive Skin ◽  
Prick Test ◽  
Inhalant Allergens

Background: Serum specific immunoglobulin E (ssIgE) sensitization to common inhalant allergens has not been studied in Indonesia. This study aimed to evaluate specific IgE production of common inhalant allergens in patients with asthma and/or allergic rhinitis in Jakarta, Indonesia.Methods: This was a cross-sectional study in adult patients with respiratory allergy from September to December 2016 at Cipto Mangunkusumo Hospital, Jakarta. Patients were included if they showed at least one positive skin prick test (SPT) to environmental allergens. Serum specific IgE was assayed by using multiple allergosorbent methods. Inhalant allergens tested were dust mites, pollen, cockroach, animal dander, and mould. Serum IgE level more than 0.35 kU/L was considered positive.Results: One hundred subjects were enrolled (76% women). Dust mites made up 75% of sensitization, followed by cat/dog (31%), cockroach (27%), pollen (24%), and mould (6%). Almost all patients sensitized to cockroach, pollen, cat/dog epithelia and mould were also co-sensitized with dust mites. Twenty two percent of patients were negative to all tested allergens.Conclusion: IgE-sensitization to inhalant allergens varies widely in respiratory allergic patients. House dust and storage mites are the most common allergens. About one-fifth of the subjects did not show specific-IgE sensitization. Thus, this test should always be combined with SPT to diagnose allergy.

scholarly journals MicroRNAs-1299, -126-3p and -30e-3p as Potential Diagnostic Biomarkers for Prediabetes

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 949
Author(s):  
Cecil J. Weale ◽  
Don M. Matshazi ◽  
Saarah F. G. Davids ◽  
Shanel Raghubeer ◽  
Rajiv T. Erasmus ◽  
...  
Keyword(s):  
Receiver Operating Characteristic ◽  
Operating Characteristic ◽  
Characteristic Curve ◽  
Predictive Ability ◽  
Roc Curves ◽  
Tolerance Test ◽  
Cross Sectional Study ◽  
Oral Glucose ◽  
Cross Sectional ◽  
Receiver Operating

This cross-sectional study investigated the association of miR-1299, -126-3p and -30e-3p with and their diagnostic capability for dysglycaemia in 1273 (men, n = 345) South Africans, aged >20 years. Glycaemic status was assessed by oral glucose tolerance test (OGTT). Whole blood microRNA (miRNA) expressions were assessed using TaqMan-based reverse transcription quantitative-PCR (RT-qPCR). Receiver operating characteristic (ROC) curves assessed the ability of each miRNA to discriminate dysglycaemia, while multivariable logistic regression analyses linked expression with dysglycaemia. In all, 207 (16.2%) and 94 (7.4%) participants had prediabetes and type 2 diabetes mellitus (T2DM), respectively. All three miRNAs were significantly highly expressed in individuals with prediabetes compared to normotolerant patients, p < 0.001. miR-30e-3p and miR-126-3p were also significantly more expressed in T2DM versus normotolerant patients, p < 0.001. In multivariable logistic regressions, the three miRNAs were consistently and continuously associated with prediabetes, while only miR-126-3p was associated with T2DM. The ROC analysis indicated all three miRNAs had a significant overall predictive ability to diagnose prediabetes, diabetes and the combination of both (dysglycaemia), with the area under the receiver operating characteristic curve (AUC) being significantly higher for miR-126-3p in prediabetes. For prediabetes diagnosis, miR-126-3p (AUC = 0.760) outperformed HbA1c (AUC = 0.695), p = 0.042. These results suggest that miR-1299, -126-3p and -30e-3p are associated with prediabetes, and measuring miR-126-3p could potentially contribute to diabetes risk screening strategies.

scholarly journals Beijing Friendship Hospital Osteoporosis Self-Assessment Tool for Elderly Male (BFH-OSTM) vs Fracture Risk Assessment Tool (FRAX) for identifying painful new osteoporotic vertebral fractures in older Chinese men: a cross-sectional study

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Ning An ◽  
Ji Sheng Lin ◽  
Qi Fei
Keyword(s):  
Assessment Tool ◽  
Roc Curves ◽  
Cross Sectional Study ◽  
Self Assessment ◽  
Cross Sectional ◽  
Fracture Group ◽  
Friendship Hospital ◽  
Fracture Risk Assessment Tool ◽  
Older Chinese ◽  
Sensitivity Specificity

Abstract Background To compare the validation of four tools for identifying painful new osteoporotic vertebral compression fractures (PNOVCFs) in older Chinese men: bone mineral density (BMD), Asian osteoporosis self-assessment tool (OSTA), World Health Organization fracture risk assessment tool (FRAX) (without BMD) and Beijing Friendship Hospital Osteoporosis Self-Assessment Tool (BFH-OSTM). Methods A cross sectional study was conducted from 2013 to 2019. A total of 846 men aged ≥50 were included and were divided into two groups: Fracture Group (patients with PNOVCFs underwent percutaneous vertebroplasty surgery) and Non-Fracture Group (community dwelled subjects for healthy examination). All subjects accepted a dual-energy X-ray BMD test and a structured questionnaire. The results of BMD, OSTA, FRAX and BFH-OSTM scores were assessed and receiver-operating characteristic (ROC) curves were generated to compare the validity of four tools for identifying PNOVCFs. Optimal cutoff points, sensitivity, specificity, and areas under the ROC curves (AUCs) were determined. Results There were significant differences including BMD T score (femoral neck, total hip and L1-L4), OSTA, FRAX and BFH-OSTM scores between Fracture group and Non-fracture group. Compared to BMD and OSTA, BFH-OSTM and FRAX had better predictive value, the sensitivity, specificity and AUC value are 0.841, 81.29%, 70.67% and 0.796, 74.85%, 78.52%, respectively. Compared with FRAX, the BFH-OSTM has a better AUC value. Conclusions Both BFH-OSTM and FRAX can be used to identify POVCFs, However, BFH-OSTM model may be a more simple and effective tool to identify the risk of POVCFs in Chinese elderly men.

scholarly journals Diagnostic accuracy of anthropometric indices in predicting excess body fat among seven to ten-year-old children

2014 ◽  
Vol 17 (1) ◽  
pp. 243-254 ◽  
Author(s):  
Edineia Aparecida Gomes Ribeiro ◽  
Danielle Biazzi Leal ◽  
Maria Alice Altenburg de Assis
Keyword(s):  
Diagnostic Accuracy ◽  
Body Fat ◽  
Roc Curves ◽  
Cross Sectional Study ◽  
The Body ◽  
Anthropometric Indices ◽  
Cross Sectional ◽  
Standardized Residuals ◽  
Highly Sensitive ◽  
Anthropometric Index

PURPOSE: To evaluate the accuracy of the body mass index (BMI), waist circumference (WC) and waist-to-height ratio (WHtR) in detecting excess body fat among schoolchildren in Florianópolis, Santa Catarina. METHODS: Cross-sectional study with 2,772 schoolchildren aged seven to ten years. Receiver Operating Characteristic (ROC) curves were used to compare the diagnostic accuracy of the BMI, the WC and the WHtR in identifying children with excess body fat (defined as values that were equal to or higher than the 90th percentile of the standardized residuals obtained from the sum of the four skinfolds thickness). Likelihood ratio estimates were used to select, for each anthropometric index, the cut-off points that presented the highest association with excess body fat. RESULTS: The BMI, WC and WHtR performed well in detecting excess body fat, indicated by areas under the ROC curve (AUC) close to 1.0, with slightly higher AUC for the BMI in comparison to the WC and the WHtR concerning both sexes. Highly sensitive and specific cut-off points were derived for the three anthropometric indices. Sensitivity ranged from 85.7 to 92.9% for the BMI, from 78.6 to 89.7% for the WC, and from 78.6 to 89.2% for the WHtR. Specificity ranged from 83.2 to 91.4%, from 75.0 to 90.7%, and from 77.4 to 88.3% for the BMI, the WC and the WHtR, respectively. CONCLUSION: BMI, WC and WHtR can be used as diagnostic tests to identify excess body fat in children from seven to ten years of age.

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