scholarly journals A success targeted nano delivery to lung cancer cells with multi-walled carbon nanotubes conjugated to bromocriptine

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fatemeh Mohammadhosseini Kamazani ◽  
Fattah Sotoodehnejad nematalahi ◽  
Seyed Davar Siadat ◽  
Majid Pornour ◽  
Mojgan Sheikhpour
Keyword(s):  
Lung Cancer ◽  
Carbon Nanotubes ◽  
Cancer Cells ◽  
Lethal Effect ◽  
Lung Cancer Cells ◽  
Expression Studies ◽  
Before And After ◽  
Multi Walled Carbon Nanotubes ◽  
Gene Expression Studies ◽  
Walled Carbon Nanotubes

AbstractIn this research, a new nano drug-based multi-walled carbon nanotubes (MWCNTs) was prepared and evaluated qualitatively. Bromocriptine (BRC) was conjugated to functionalized carbon nanotubes. Then, the CHNS, FT-IR, SEM, and RAMAN tests for characterization of the conjugated drug were done. The nanofluid-containing nano-drug was evaluated on lung cancer cells (A549 & QU-DB) and MRC5 by MTT and flow cytometry tests. Then, the gene expression studies of dopamine receptor genes were done before and after nano-drug treatment. After that, a western blotting test was carried out for further investigation of dopamine receptors protein production. Finally, Bax and Bcl-2 secretion were measured by the ELISA method in cells affected by MWCNTs-BRC Nf compared to untreated cells. The results showed that the nano-drug had a significant lethal effect on cancer cells, while it had no toxicity on MRC5. Also, the nano-drug could significantly induce apoptosis in lung cancer cells at a lower dose compared to the drug alone. In this study, a targeted nano-drug delivery system was designed, and its performance was evaluated based on neurotransmitter pathways, and the results showed that it may be useful in the treatment of lung cancer. However, additional studies on animal models are underway.

scholarly journals Multi-Walled Carbon Nanotubes Decorated with Guanidinylated Dendritic Molecular Transporters: An Efficient Platform for the Selective Anticancer Activity of Doxorubicin

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 858
Author(s):  
Kyriaki-Marina Lyra ◽  
Archontia Kaminari ◽  
Katerina N. Panagiotaki ◽  
Konstantinos Spyrou ◽  
Sergios Papageorgiou ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Delivery System ◽  
Colloidal Stability ◽  
Triggered Release ◽  
Selective Toxicity ◽  
Molecular Transporters ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

An efficient doxorubicin (DOX) drug delivery system with specificity against tumor cells was developed, based on multi-walled carbon nanotubes (MWCNTs) functionalized with guanidinylated dendritic molecular transporters. Acid-treated MWCNTs (oxCNTs) interacted both electrostatically and through hydrogen bonding and van der Waals attraction forces with guanidinylated derivatives of 5000 and 25,000 Da molecular weight hyperbranched polyethyleneimine (GPEI5K and GPEI25K). Chemical characterization of these GPEI-functionalized oxCNTs revealed successful decoration with GPEIs all over the oxCNTs sidewalls, which, due to the presence of guanidinium groups, gave them aqueous compatibility and, thus, exceptional colloidal stability. These GPEI-functionalized CNTs were subsequently loaded with DOX for selective anticancer activity, yielding systems of high DOX loading, up to 99.5% encapsulation efficiency, while the DOX-loaded systems exhibited pH-triggered release and higher therapeutic efficacy compared to that of free DOX. Most importantly, the oxCNTs@GPEI5K-DOX system caused high and selective toxicity against cancer cells in a non-apoptotic, fast and catastrophic manner that cancer cells cannot recover from. Therefore, the oxCNTs@GPEI5K nanocarrier was found to be a potent and efficient nanoscale DOX delivery system, exhibiting high selectivity against cancerous cells, thus constituting a promising candidate for cancer therapy.

pH Responsive Release of Doxorubicin to the Cancer Cells by Functionalized Multi-Walled Carbon Nanotubes

2015 ◽  
Vol 15 (7) ◽  
pp. 4799-4805 ◽  
Author(s):  
B. Anbarasan ◽  
S. Vignesh Babu ◽  
K. Elango ◽  
B. Shriya ◽  
S. Ramaprabhu
Keyword(s):  
Carbon Nanotubes ◽  
Cancer Cells ◽  
Ph Responsive ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Purification of Multi-Walled Carbon Nanotubes Grown by Thermal CVD on Fe-Based Catalyst

2006 ◽  
Vol 48 ◽  
pp. 50-54 ◽  
Author(s):  
Th. Dikonimos Makris ◽  
L. Giorgi ◽  
R. Giorgi ◽  
Nicola Lisi ◽  
Elena Salernitano ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Catalyst Support ◽  
Secondary Phase ◽  
Metal Catalyst ◽  
Purification Method ◽  
Thermal Cvd ◽  
Aluminum Ions ◽  
Before And After ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Aiming at the purpose of using carbon nanotubes as secondary phase in composite materials, removal of metal catalyst, catalyst support and amorphous carbon is crucial to make the most of the required properties. A purification method was developed to remove the metal catalyst from multi-walled nanotubes grown by thermal CVD. A nanosized Fe-based catalyst, prepared by coprecipitation of iron and aluminum ions, followed by solid state reaction, was used to catalyze the growth. Carbon nanotubes were subjected to acid purification and a comparison between nitric acid and a mixture of nitric and hydrochloric acid for the removal of Fe and Fe oxides is provided. Morphological and spectroscopic analyses of the materials were performed, both before and after the purification processes.

Comparative study of electrochemical capacitance of multi-walled carbon nanotubes before and after chopping

2010 ◽  
Vol 257 (2) ◽  
pp. 440-445 ◽  
Author(s):  
Changzhou Yuan ◽  
Laifa Shen ◽  
Diankai Li ◽  
Fang Zhang ◽  
Xiangjun Lu ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Comparative Study ◽  
Electrochemical Capacitance ◽  
Before And After ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

scholarly journals Combining Carbon Nanotubes and Chitosan for the Vectorization of Methotrexate to Lung Cancer Cells

Materials ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 2889 ◽  
Author(s):  
Giuseppe Cirillo ◽  
Orazio Vittorio ◽  
David Kunhardt ◽  
Emanuele Valli ◽  
Florida Voli ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Carbon Nanotubes ◽  
Photoelectron Spectroscopy ◽  
Ph Responsive ◽  
Carbon Nanostructure ◽  
X Ray ◽  
Transmission Electron ◽  
Multi Walled Carbon Nanotubes ◽  
Responsive Behavior ◽  
Walled Carbon Nanotubes

A hybrid system composed of multi-walled carbon nanotubes coated with chitosan was proposed as a pH-responsive carrier for the vectorization of methotrexate to lung cancer. The effective coating of the carbon nanostructure by chitosan, quantified (20% by weight) by thermogravimetric analysis, was assessed by combined scanning and transmission electron microscopy, and X-ray photoelectron spectroscopy (N1s signal), respectively. Furthermore, Raman spectroscopy was used to characterize the interaction between polysaccharide and carbon counterparts. Methotrexate was physically loaded onto the nanohybrid and the release profiles showed a pH-responsive behavior with higher and faster release in acidic (pH 5.0) vs. neutral (pH 7.4) environments. Empty nanoparticles were found to be highly biocompatible in either healthy (MRC-5) or cancerous (H1299) cells, with the nanocarrier being effective in reducing the drug toxicity on MRC-5 while enhancing the anticancer activity on H1299.

T4 Reduces Cisplatin Resistance by Inhibiting AEG-1 Gene Expression in Lung Cancer Cells

2020 ◽  
Author(s):  
Tianjiao Song ◽  
Xiaohong Lin ◽  
Pingting Huang ◽  
Yuqing Chen ◽  
Limin Chen
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Cisplatin Resistance ◽  
A549 Cells ◽  
Lethal Effect ◽  
Lung Cancer Cells ◽  
Chemotherapeutic Drugs ◽  
Lung Cancer Patients ◽  
Chemotherapy Drugs ◽  
Mdr 1

Abstract BackgroundLung cancer is one of the deadliest diseases in the world. Most lung cancer patients are resistant to chemotherapy drugs. In our study, we investigated whether T4 can reduce the resistance of lung cancer cells to chemotherapeutic drugs through the action of AEG-1.Materials and Methods1.A549 and A549/DDP cells were respectively transfected with overexpressing AEG-1 and knockdown AEG-1 plasmid. A549 and A549/DDP cells were added 0、25、50、100、200nM T4 respectively. 200nM T4 was selected for following experiments. A549/DDP cells were divided into A549/DDP empty group, T4 group, T4+AEG-1 overexpressing group. CCK8 assay was used to detect the proliferation of cells in each group. RT-qPCR and Western blotting were used to detect the expression of AEG-1 and MDR-1.ResultsAs expected, the expression of AEG-1 in A549 and A549/DDP cells is positively correlated with cisplatin resistance. When AEG-1 protein was overexpressed in A549 cells, the lethal effect of cisplatin on A549 cells was attenuated (all P<0.05). After AEG-1 protein was knocked down in A549/DDP cells, cisplatin was applied to the A549/DDP cells. The lethal effect was significantly increased compared to that in the control cells (all P<0.05). The expression of AEG-1 protein gradually decreased with increasing concentration of T4 in A549 and A549/DDP cells; The resistance to cisplatin was reduced after the addition of T4 to A549/DDP cells (P<0.05), and this effect was enhanced after transfection with the AEG-1 plasmid. ConclusionIn summary, T4 is important for increasing the sensitively of lung cancer cells to cisplatin. AEG-1 may be a key protein involved in this effect and may have an important impact on the survival rate of chemotherapy in patients with lung cancer in the future.

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