scholarly journals Geographic source estimation using airborne plant environmental DNA in dust

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chelsea Lennartz ◽  
Joel Kurucar ◽  
Stephen Coppola ◽  
Janice Crager ◽  
Johanna Bobrow ◽  
...  
Keyword(s):  
Plant Species ◽  
Fungal Spores ◽  
Geographic Origin ◽  
Microscopic Analysis ◽  
Environmental Dna ◽  
Dust Sample ◽  
Accurate Estimate ◽  
Plant Parts ◽  
Settled Dust ◽  
Great Utility

AbstractInformation obtained from the analysis of dust, particularly biological particles such as pollen, plant parts, and fungal spores, has great utility in forensic geolocation. As an alternative to manual microscopic analysis of dust components, we developed a pipeline that utilizes the airborne plant environmental DNA (eDNA) in settled dust to estimate geographic origin. Metabarcoding of settled airborne eDNA was used to identify plant species whose geographic distributions were then derived from occurrence records in the USGS Biodiversity in Service of Our Nation (BISON) database. The distributions for all plant species identified in a sample were used to generate a probabilistic estimate of the sample source. With settled dust collected at four U.S. sites over a 15-month period, we demonstrated positive regional geolocation (within 600 km2 of the collection point) with 47.6% (20 of 42) of the samples analyzed. Attribution accuracy and resolution was dependent on the number of plant species identified in a dust sample, which was greatly affected by the season of collection. In dust samples that yielded a minimum of 20 identified plant species, positive regional attribution was achieved with 66.7% (16 of 24 samples). For broader demonstration, citizen-collected dust samples collected from 31 diverse U.S. sites were analyzed, and trace plant eDNA provided relevant regional attribution information on provenance in 32.2% of samples. This showed that analysis of airborne plant eDNA in settled dust can provide an accurate estimate regional provenance within the U.S., and relevant forensic information, for a substantial fraction of samples analyzed.

scholarly journals Estimation of Geographic Origin from Dust Using Plant DNA Metabarcoding

2021 ◽  
Author(s):  
Chelsea Lennartz ◽  
Joel Kurucar ◽  
Stephen Coppola ◽  
Janice Crager ◽  
Johanna Bobrow ◽  
...  
Keyword(s):  
Plant Species ◽  
Fungal Spores ◽  
Geographic Origin ◽  
Microscopic Analysis ◽  
Environmental Dna ◽  
Dust Sample ◽  
Accurate Estimate ◽  
Plant Parts ◽  
Great Utility ◽  
Previous Sample

Abstract Information obtained from the analysis of dust, particularly biological particles such as pollen, plant parts, and fungal spores, has great utility in forensic geolocation. As an alternative to manual microscopic analysis, we developed a pipeline that utilizes the environmental DNA (eDNA) from plants in dust samples to estimate previous sample location(s). The species of plant-derived eDNA within dust samples were identified using metabarcoding and their geographic distributions were then derived from occurrence records in the USGS Biodiversity in Service of Our Nation (BISON) database. The distributions for all plant species identified in a sample were used to generate a probabilistic estimate of the sample source. With settled dust collected at four U.S. sites over a 15-month period, we demonstrated positive regional geolocation (within 600 km 2 of the collection point) with 47.6% (20 of 42) of the samples analyzed. Attribution accuracy and resolution was dependent on the number of plant species identified in a dust sample, which was greatly affected by the season of collection. In dust samples that yielded a minimum of 20 identified plant species, positive regional attribution improved to 66.7% (16 of 24 samples). Using dust samples collected from 31 different U.S. sites, trace plant eDNA provided relevant regional attribution information on provenance in 32.2%. This demonstrated that analysis of plant eDNA in dust can provide an accurate estimate regional provenance within the U.S., and relevant forensic information, for a substantial fraction of samples analyzed.

Medicinal plants and sustainable livelihood in Pauri district of Garhwal Himalaya, Uttarakhand, India.

2016 ◽  
Vol 5 (06) ◽  
pp. 4589 ◽  
Author(s):  
Vardan Singh Rawat
Keyword(s):  
Environmental Management ◽  
Medicinal Plants ◽  
Plant Species ◽  
Skin Diseases ◽  
Sustainable Livelihoods ◽  
Vital Role ◽  
Local Communities ◽  
Sustainable Livelihood ◽  
Plant Parts ◽  
Total Plant

The present study was conducted in the Thalisain block of Pauri Garhwal to document the medicinal plants used by the local communities. 53 plant species distributed in 38 families were documented. Of the total plant species 49% were herbs, 26% trees, 23% shrubs and 2% climbers. 16 different plant parts were used by local communities for different ailments. Medicinal plants were widely used by major sections of the community against common colds, cough, skin diseases, snake bite, fever, joint pains, bronchitis etc. Women and local healers called vaids have a vital role in environmental management due to traditional knowledge and use of plants as medicine with undocumented knowledge. It has been observed as one of the best option of sustainable livelihoods for the residents of the area.

Field Observations of Oviposition by a Specialist Herbivore on Plant Parts and Plant Species Unsuitable as Larval Food

2011 ◽  
Vol 40 (6) ◽  
pp. 1478-1486 ◽  
Author(s):  
Nicole D. Benda ◽  
Cavell Brownie ◽  
Coby Schal ◽  
Fred Gould
Keyword(s):  
Plant Species ◽  
Field Observations ◽  
Larval Food ◽  
Specialist Herbivore ◽  
Plant Parts

Medicinal Plants of Morni Hills, Shivalik Range, Panchkula, Haryana

2018 ◽  
Vol 25 (1) ◽  
pp. 1-14
Author(s):  
Acharya Balkrishna ◽  
◽  
Anupam Srivastava ◽  
B.K. Shukla ◽  
Rajesh Kumar Mishra ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Plant Species ◽  
Field Trips ◽  
Local People ◽  
Medicinal Uses ◽  
Plant Parts ◽  
Plant Exploration ◽  
Family Names ◽  
Medicinal Properties ◽  
Hills Area

During plant exploration and survey of Morni Hills, Panchkula, Haryana (2017-2018) the authors collected about 2200 field numbers from different localities. Out of them, 323 species belonging to 251 genera and 92 families are medicinal plants. The information about medicinal properties of these plants has been gathered during field trips of Morni Hills from local vaidyas and local people of remote localities. The enumeration is alphabetically arranged, followed by their family names, local/common names, plant parts used for curing diseases and medicinal uses. These plant species are utilized by local people against various diseases in Morni Hills area.

scholarly journals Ethnobotanical study of medicinal plants of Resunga hill used by Magar community of Badagaun VDC, Gulmi district, Nepal

2012 ◽  
Vol 10 (10) ◽  
pp. 54-65 ◽  
Author(s):  
Rajendra Acharya
Keyword(s):  
Medicinal Plants ◽  
Respiratory Tract ◽  
Plant Species ◽  
Stem Bark ◽  
Traditional Healers ◽  
Easy Access ◽  
Ethnobotanical Study ◽  
Young Generation ◽  
Plant Parts ◽  
Scientific World

Present study aims to explore the ethnobotanical information on medicinal plants used by Magar community of Badagaun VDC of Gulmi district. The study was carried out during different periods of the year 2010. Informations on plant and plant parts uses were collected interviewing key informants by using semi-structured open-ended questionnaire. Altogether 161 different plant species belonging to 87 familes and 144 genera were documented and majority of them are herbs. In terms of plant parts use, leaf and stem/bark are in top priorities. These plants are used to treat different ailments ranging from gastro-intestinal to headache and fever, respiratory tract related problems to dermatological problems, snake bite to ophthalmic and cuts and wounds. Easy access to modern medicines and less recognition of traditional healers are the main causes leading to decrease in interest of young generation in the use of traditional medicinal practices. Scientific World, Vol. 10, No. 10, July 2012 p54-65 DOI: http://dx.doi.org/10.3126/sw.v10i10.6863

scholarly journals Age, wound size and position of injury – dependent vascular regeneration assay in growing leaves

2020 ◽  
Author(s):  
Dhanya Radhakrishnan ◽  
Anju Pallipurath Shanmukhan ◽  
Abdul Kareem ◽  
Mabel Maria Mathew ◽  
Vijina Varaparambathu ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Molecular Mechanism ◽  
Rapid Method ◽  
Plant Species ◽  
Plant Parts ◽  
Vascular Regeneration ◽  
New Findings ◽  
Stepwise Method ◽  
Experimental Approaches ◽  
Regeneration Efficiency

ABSTRACTBackgroundRecurring damage to aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assay in aerial plant parts such as stem and inflorescence stalk are well established, those on leaf vasculature remained unexplored. Recently we established a new vascular regeneration assay in growing leaf and discovered the underlying molecular mechanism.ResultsHere we describe the detailed stepwise method of incision and the regeneration assay used for studying the leaf vascular regeneration. By using a combination of micro-surgical perturbations, brightfield microscopy and other experimental approaches, our new findings show that the regeneration efficiency decreases with aging of the leaf, and increases with the nearness of the wound towards the proximal end of the leaf.ConclusionThis easy-to-master vascular regeneration assay is an efficient and rapid method to study the mechanism of vascular regeneration in growing leaves. It can be readily adapted for other plant species and can be combined with cellular and molecular biology techniques.

scholarly journals Studies on the arbuscular mycorrhizal fungal association in some medicinal plant species of paithal hills, western ghats kannur district, kerala

2020 ◽  
Vol 7 (2) ◽  
pp. 30-38
Author(s):  
Santhoshkumar S ◽  
Nagarajan N ◽  
Sree Priya S
Keyword(s):  
Plant Species ◽  
Root Colonization ◽  
Rhizosphere Soil ◽  
Fungal Spores ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Fungal Colonization ◽  
The Family ◽  
Spore Population ◽  
Arbuscular Mycorrhizal Fungal

In the present study to analyzed that the arbuscular mycorrhizal fungal spores in root colonization and spore population in rhizosphere soils samples in various medicinal at Paithal hills,Western Ghats of Kannur district, Kerala, India. Root and rhizosphere soil samples were collected during the month of August, 2018-March, 2019 from the surface to 30 cm depth as well as pH were also recorded. Totally 30 plant species belonging to 19 families were collected and identified. The present result showed arbuscular mycorrhizal spore population in the rhizosphere soil and root colonization of all the plant species. A total of 19 AM fungal spores were recovered from the rhizosphere soil samples in this study region. The Glomus was dominant had seen in rhizosphere soil samples in all the medicinal plant species. The maximum spore population was found in the rhizosphere soil samples of Mimosa pudica (590/100g of soil) which belongs to the family Mimosaceae and the lowest spore population was observed in the Terminalia bellirica 135/100g of soil) belongs to Combretaceae family. The highest  78 % AM fungal colonization was found in roots of Euphorbia hirta belongs to the family Euphorbiaceae. While the lowest 11 % AM fungal colonization was found in the root of Sida acuta belongs to the family Malvaceae.

scholarly journals Medicinal plants used by tribal and rural community of Sata Pokhran area of tehsil Pampore of district Pulwama, Jammu and Kashmir-India

2018 ◽  
Vol 7 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Mohd Shahijahan Dar ◽  
◽  
GH. Nabi Joo ◽  
Showkat Ahmad Ganaie ◽  
◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Plant Species ◽  
Rural Community ◽  
Jammu And Kashmir ◽  
Medicinal Uses ◽  
Mode Of Administration ◽  
Plant Parts ◽  
Family Names ◽  
Whole Plant

Man has used plants since time immemorial to alleviate sufferings and ailments. The present study attempts to explore and document the medicinal plants used against several diseases by the tribal and rural community of Sata Pokhran area of district Pulwama. During the survey from March to September 2017, a total of 28 different plant species belonging to equal number of genera and 16 different families were found to be used as effective remedies. The present study revealed that either whole plant or some plant parts are used to cure different diseases. It was also observed that majority of plant species are used to treat more than one disease and likewise more than one plant species is used to treat a particular disease. The scientific, local and family names of these medicinal plants along with their parts used, mode of administration and ethno medicinal uses are presented in this paper

scholarly journals Identification and documentation of Ethno-veterinary remedies used by Afar Pastoralists for the treatment of Camel diseases in Ethiopia

2021 ◽  
Author(s):  
Fikadu Gutema ◽  
Weldegebrial G.Aregawi ◽  
Juhar Tesfaye Bekele ◽  
Abel sorsa Geletu
Keyword(s):  
Medicinal Plants ◽  
Plant Species ◽  
Animal Health ◽  
Traditional Healers ◽  
Herbal Remedies ◽  
Phytochemical Analysis ◽  
Ethnoveterinary Medicine ◽  
Plant Parts ◽  
Afar Region ◽  
Plant Nursery

Abstract Background: Ethnoveterinary medicine is a scientific term for traditional animal health care that encompasses knowledge, belief, practice and skill of the community used for curing diseases and maintaining health of animals. A cross-sectional study was conducted using semi-structured questionnaires from September 2016 to August 2017 to gather information on the knowledge, practice and skill of the pastoralists and traditional healers about the importance and application of Ethno-veterinary medicine. Moreover, this study was conducted with the objectives of identification and documentation of major ethno-veterinary medicinal plants commonly used to treat camel aliments in Afar region.Result: A total of 146 traditional healers and pastoralists were interviewed for baseline and reliable information about Ethno-veterinary medicinal practice in Afar region. Accordingly, 99.3% of the respondents were men in which 66.44% of them were in the age range of 45-60. Majorities (76.02%) of the information’s concerning EVM were obtained from the elders among which 84.93% responded that the overall EVM practice and status in pastoral society of Afar is decreasing. A total of 710 plant species supposed to have medicinal property were recommended by pastoralists to be used for the treatment of 49 different animal diseases. Among 64 plants specimen collected, Balanites aegyptia, Cissus rotundifolia (Forssk.) Vahl, Cadaba rotundifolia Forssk, Solanum sp, Acalypha indica L and Tamarix aphylla (L.) Karsts were the commonly used plant species with high fidelity value. Concerned with plant parts used, leaf (20.5%) was the most frequently used plant parts followed by root (19.4%), fruit (14.4%), seed (10.8%) and entire plant (8.8%). These herbal remedies were administered orally, nasally, ocular and topically.Conclusion: pastoralists and traditional healers in Afar region have immense indigenous knowledge and practices of treating sick animals which should be supported by scientific techniques for analyzing the active ingredients contained in each plant species and for evaluating the safety, efficacy and dosage of the commonly used medicinal plants. In addition, on station establishment of commonly used medicinal plant nursery for conservation purpose and to conduct in-depth phytochemical analysis is paramount important.

scholarly journals Medicinal Plants from Vettangudi Water Bird Sanctuary in Sivagangai District of Tamil Nadu, Southern India

2021 ◽  
Vol 11 (2) ◽  
pp. 135-140
Author(s):  
S Shanmugam ◽  
C P Muthupandi ◽  
V M Eswaran ◽  
K Rajendran
Keyword(s):  
Medicinal Plants ◽  
Plant Species ◽  
Tamil Nadu ◽  
Medicinal Uses ◽  
Traditional Medicines ◽  
Water Bird ◽  
Plant Parts ◽  
Healthcare Needs ◽  
The People ◽  
Exploitation And Utilization

Most of the people depending on traditional medicine to meet their primary healthcare needs. Documenting the indigenous knowledge through ethnobotanical studies is important for the conservation of biological resources as well as their sustainable utilization. It is also necessary to collect the information about the knowledge of traditional medicines before it is permanently lost. Having all these facts in mind, the present study was carried out to document the plants used as medicine by the people inhabiting around the Vettangudi Water Bird Sanctuary of Sivagangai district in Tamil Nadu, India. The field survey was conducted in two villages situated near to Vettangudi Water Bird Sanctuary. The medicinal uses of 40 angiospermic plant species belonging to 36 genera of 24 families for various diseases and ailments were recorded by this study. The people inhabiting in the study area used 45 herbal therapies prepared from 40 plants to treat 27 different illnesses. Regarding the plant parts used, leaf was the mostly used plant part (51.16%) and extract was found as mostly followed mode (42.28%) to treat a particular disease. Attention should be made on proper exploitation and utilization of these medicinally important plant species. Keywords: Medicinal plants, Vettangudi Water Bird Sanctuary, Sivagangai district, Tamil Nadu.

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