An approach for quantitative analysis of vitamins D and B9 and their metabolites in human biofluids by on-line orthogonal sample preparation and sequential mass spectrometry detection

The Analyst ◽  
2013 ◽  
Vol 138 (7) ◽  
pp. 2146 ◽  
Author(s):  
Carlos Ferreiro-Vera ◽  
Feliciano Priego-Capote ◽  
María Dolores Luque de Castro
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Sample Preparation ◽  
Mass Spectrometry Detection ◽  
On Line ◽  
Sequential Mass Spectrometry ◽  
Analysis Of Vitamins

scholarly journals Mass Spectrometry-Based Metabolomics Combined with Quantitative Analysis of the Microalgal Diatom (Chaetoceros calcitrans)

Marine Drugs ◽  
2020 ◽  
Vol 18 (8) ◽  
pp. 403
Author(s):  
Awanis Azizan ◽  
M. Maulidiani ◽  
Rudiyanto R. ◽  
Khozirah Shaari ◽  
Intan Safinar Ismail ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Plant Species ◽  
High Resolution Mass Spectrometry ◽  
Land Plant ◽  
Mass Spectrometry Detection ◽  
Orbitrap Mass Spectrometry ◽  
Metabolic Activities ◽  
Chaetoceros Calcitrans ◽  
Acetone Extracts

Although many metabolomics studies of higher land plant species have been conducted, similar studies of lower nonland plant species, which include microalgae, are still developing. The present study represents an attempt to characterize the metabolic profile of a microalgal diatom Chaetoceros calcitrans, by applying high-resolution mass spectrometry detection, via Q-ExactiveTM Plus Orbitrap mass spectrometry. The results showed that 54 metabolites of various classes were tentatively identified. Experimentally, the chloroform and acetone extracts were clearly distinguished from other solvent extracts in chemometric regression analysis using PLS, showing the differences in the C. calcitrans metabolome between the groups. In addition, specific metabolites were evaluated, which supported the finding of antioxidant and anti-inflammatory activities. This study also provides data on the quantitative analysis of four carotenoids based on the identification results. Therefore, these findings could serve as a reliable tool for identifying and quantifying the metabolome that could reflect the metabolic activities of C. calcitrans.

Sample preparation approaches for qualitative and quantitative analysis of lipid-derived electrophile modified proteomes by mass spectrometry

2020 ◽  
Vol 16 (6) ◽  
pp. 511-520
Author(s):  
Wenjuan Yuan ◽  
Jun Wang ◽  
Ying Zhang ◽  
Haojie Lu
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Sample Preparation ◽  
Analytical Methods ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative

MS-based analytical methods of lipid-derived electrophile modified proteome are discussed in this review.

scholarly journals Mass spectrometry–based proteomics in cell biology

2010 ◽  
Vol 190 (4) ◽  
pp. 491-500 ◽  
Author(s):  
Tobias C. Walther ◽  
Matthias Mann
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
High Resolution ◽  
Sample Preparation ◽  
Cell Biology ◽  
Computational Analysis ◽  
Global Analysis ◽  
Protein Composition ◽  
Broad Community

The global analysis of protein composition, modifications, and dynamics are important goals in cell biology. Mass spectrometry (MS)–based proteomics has matured into an attractive technology for this purpose. Particularly, high resolution MS methods have been extremely successful for quantitative analysis of cellular and organellar proteomes. Rapid advances in all areas of the proteomic workflow, including sample preparation, MS, and computational analysis, should make the technology more easily available to a broad community and turn it into a staple methodology for cell biologists.

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