HPTLC-densitometric determination and kinetic studies on antioxidant potential of monomeric phenolic acids (MPAs) from Bergenia species

RSC Advances ◽  
2014 ◽  
Vol 4 (95) ◽  
pp. 52647-52657 ◽  
Author(s):  
Nishi Srivastava ◽  
Amit Srivastava ◽  
S. Srivastava ◽  
A. K. S. Rawat ◽  
A. R. Khan
Keyword(s):  
Gallic Acid ◽  
Phenolic Acids ◽  
Vanillic Acid ◽  
Protocatechuic Acid ◽  
Kinetic Studies ◽  
Syringic Acid ◽  
Antioxidant Potential ◽  
Hptlc Method

We developed a HPTLC method for the quantification of vanillic acid, syringic acid, gallic acid and protocatechuic acid and kinetic studies on antioxidant potential in Bergenia ciliata and Bergenia stracheyi.

scholarly journals Phytochemical Investigations of Laurel Fruits (Laurus nobilis)

2019 ◽  
Vol 14 (8) ◽  
pp. 1934578X1986887
Author(s):  
Zhana Petkova ◽  
Galina Stefanova ◽  
Tania Girova ◽  
Ginka Antova ◽  
Magdalena Stoyanova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Acids ◽  
Vanillic Acid ◽  
Lipid Fraction ◽  
Syringic Acid ◽  
Dominant Group ◽  
Laurus Nobilis ◽  
Lipid Fractions ◽  
Dimorphic Yeast ◽  
Valuable Compounds

Bay laurel ( Laurus nobilis L.) is an evergreen tree. The objective of this study was to determine the chemical composition (polyphenols, essential oil [EO], lipid fraction, cellulose, and protein content) of laurel fruits collected from Greece (Mount Athos) and Georgia (the village of Meria), and to evaluate the antimicrobial activity of laurel fruit EOs. The major phenolic acids in the fruits from Greece were p-coumaric acid (free 261.6 µg/g) and vanillic acid (free 253.1 µg/g and conjugated 925.8 µg/g). The major phenolic acids in fruits from Georgia were vanillic acid (free 105.6 µg/g and caffeic acid [conjugated 439.2 µg/g], and syringic acid [conjugated 390.7 µg/g]). The laurel fruit EOs from Greece (1.4% content) and Georgia (1.6%) had distinct composition. Monoterpene hydrocarbons were the dominant group of compounds in the EOs, with 49.7% in the EO from Greece and 68.7% in the EO from Georgia. The major constituents of the fruit EO from Greece were 1,8-cineole (18.2%), α-phellandrene (15.0 %), β-pinene (9.4%), and α -pinene (9.1%), whereas the ones from Georgia were trans-β-ocimene (59.4%) and 1,8-cineole (7.6%). Laurel fruit EO from Greece and Georgia demonstrated low to moderate antimicrobial activity against pathogenic and spoilage microorganisms and the dimorphic yeast Candida albicans. The main fatty acids (FAs) in the lipid fractions were oleic, palmitic, and linoleic; there were differences in FA composition between the shells and the seeds of the fruits from the two countries. γ-Тocopherol predominated in the tocopherol fraction of the lipids from fruits shells and seeds from Greece (65.3% and 54.4%, respectively), whereas β-tocopherol predominated in fruits shells and seeds from Georgia (93.7% and 45.6%, respectively). Currently underutilized, the laurel fruits from both Greece and Georgia contain various valuable compounds that may potentially be used for perfumery, cosmetic, and pharmaceutical applications.

Simultaneous analytical efficiency evaluation using HPTLC method for analysis of syringicacid and vanillic acid and their anti-oxidant capacity from methanol extract of Ricinus communis Linn. and Euphorbia hirta Linn

2020 ◽  
Author(s):  
Pradeep Singh ◽  
Muhammad Arif ◽  
Abdul Qadir ◽  
Pushpendra Kannojia
Keyword(s):  
Antioxidant Activity ◽  
Vanillic Acid ◽  
Methanol Extract ◽  
Ricinus Communis ◽  
Radical Scavenging ◽  
Syringic Acid ◽  
Euphorbia Hirta ◽  
Anti Oxidant ◽  
Marker Compounds ◽  
Hptlc Method

Abstract Background Well-known medicinal plants Ricinus communis Linn. and Euphorbia hirta Linn. have been traditionally used to treat a variety of ailments in the India and other countries. Objective In present research, a simple, rapid, reliable and accessible HPTLC technique has been established for concurrent quantification of phenolic compounds (syringic acid and vanillic acid)and evaluation of their anti-oxidant capacity. Method The chromatographic separation was conceded on pre-coated silica gel plates F254 as the stationary phase. The appropriate mobilephasecombination toluene: ethyl acetate: formic acid (7:2.5:0.5 v/v/v) were developed to expand the plates which separated components according to the marker compounds. Further, the anti-oxidant potential of methanol extract R. communis (MERC) and E. hirta (MERH) were also assessed with (DPPH) by using UV spectrophotometer. Results Densitometric scanning was performed by Camag scanner V and measured at two different wave length 272 nm and 318 nm. The marker compounds were practically resolved with RF 0.5 ± 0.04 for syringic acid and 0.6 ± 0.06 for vanillic acid. The results obtained in the study of anti-oxidant activity of methanol extract R. communis(MERC) and E. hirta (MERH) were showed significant free radical scavenging capacity against DPPH generated free radicals. Conclusions The developed HPTLC method was validated for accuracy, linearity, precision, and specificity. Both the extract has revealed considerable antioxidant activity. The reported existing phenolic and flavonoids compounds are responsible for antioxidant activity of plant extract.

scholarly journals Anti-malarial Activity Of Phenolic Acids Is Structurally Related

2020 ◽  
Author(s):  
Prince A Fordjour ◽  
Jonathan P Adjimani ◽  
Bright Asare ◽  
Nancy O Duah-Quashie ◽  
Neils B Quashie
Keyword(s):  
Plasmodium Falciparum ◽  
Ferulic Acid ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Protocatechuic Acid ◽  
Antimalarial Activity ◽  
Coumaric Acid

Abstract Background In the absence of an effective vaccine against malaria, chemotherapy remains a major option in the control of the disease. Then, the recent report of the emergence and spread of clones of Plasmodium falciparum resistant to available antimalarial drugs should be of concern as it poses a threat to disease control. Compounds whose pharmacological properties have been determined and touted for other disease can be investigated for antimalarial activity. Phenolic acids (polyphenols) have been reported to exhibit antioxidant, anticancer, anti-inflammatory, antiviral and antibiotic effects. However, information on their antimalarial activity is scanty. Phenolic acids are present in a variety of plant-based foods: mostly high in the skins and seeds of fruits as well as the leaves of vegetables. Systematic assessment of these compounds for antimalarial activity is therefore needed. Method Using the classical in vitro drug test, the antimalarial activities of five hydroxycinnamic acids, (caffeic acid, rosmarinic acid, chlorogenic acid, o-Coumaric acid and ferulic acid) and two hydroxybenzoic acids (gallic acid and protocatechuic acid) against 3D7 clones of Plasmodium falciparum was determined. Results Among the phenolic acids tested, caffeic acid and gallic acid were found to be the most effective, with mean IC 50 value of 17.73µg/ml and 26.59µg/ml respectively for three independent determinations. Protocatechuic acid had an IC 50 value of 30.08 µg/ml. Rosmarinic acid and chlorogenic acid, showed moderate antimalarial activities with IC 50 values of 103.59µg/ml and 105µg/ml respectively. The IC 50 values determined for ferulic acid and o-Coumaric acid were 93.36µg/ml and 82.23µg/ml respectively. Conclusion The outcome of this study suggest that natural occurring phenolic compounds have appreciable level of antimalarial activity which can be exploited for use through combination of actions/efforts including structural manipulation to attain an increase in their antimalarial effect. Eating of natural food products rich in these compounds could provide antimalarial prophylactic effect.

scholarly journals DER ABBAU VON P-HYDROXYBENZOESAURE, PROTOCATECHUSAURE UND GALLUSSAURE IM BODEN

2016 ◽  
Vol 6 ◽  
Author(s):  
Christian Kunze
Keyword(s):  
Gallic Acid ◽  
Phenolic Acids ◽  
Protocatechuic Acid ◽  
Hydroxybenzoic Acid ◽  
Microbial Decomposition ◽  
Oh Groups

The rate of decomposition of the three phenolic acids, p-hydroxybenzoic acid, protocatechuic acid and gallic acid, whose difference is the amount of OH-groups, was investigated in the soil. With the increase in OH-groups increases the rate of microbial decomposition. Gallic acid decomposes faster than protocatecuic acid and this again faster than p-hydroxybenzoic acid.

scholarly journals Antioxidant constituents of three selected red and green color Amaranthus leafy vegetable

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Umakanta Sarker ◽  
Shinya Oba
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Protocatechuic Acid ◽  
Pharmacological Study ◽  
Correlation Study ◽  
Syringic Acid ◽  
Coumaric Acid ◽  
Green Color ◽  
Red Color ◽  
Excellent Source

AbstractRed color (A. tricolor) genotypes are an excellent source of pigments, such as betalain (1122.47 ng g−1 FW), β-xanthin (585.22 ng g−1 FW), β-cyanin (624.75 ng g−1 FW), carotenoids (55.55 mg 100 g−1 FW), and antioxidant phytochemicals, such as vitamin C (122.43 mg 100 g−1 FW), TFC (312.64 RE µg g−1 DW), TPC (220.04 GAE µg g−1 DW), TAC (DPPH and ABTS+) (43.81 and 66.59 TEAC µg g−1 DW) compared to green color (A. lividus) genotype. Remarkable phenolic acids, such as salicylic acid, vanillic acid, protocatechuic acid, gallic acid, gentisic acid, β-resorcylic acid, p-hydroxybenzoic acid, syringic acid, ellagic acid, chlorogenic acid, sinapic acids, trans-cinnamic acid, m-coumaric acid, caffeic acid, p-coumaric acid, ferulic acid, and flavonoids, such as rutin, hyperoside, isoquercetin, myricetin, quercetin, apigenin, kaempferol, and catechin were observed in the red color amaranth genotypes, which was much higher compared to the green color amaranth genotype. We newly identified four flavonoids such as quercetin, catechin, myricetin, and apigenin in amaranth. Among the three selected advanced genotypes studied the red color genotype VA13 and VA3 had abundant antioxidant pigments, phytochemicals, phenolic acids, flavonoids, and antioxidant activity could be selected for extracting colorful juice. Correlation study revealed that all antioxidant constituents of red color amaranth had strong antioxidant activity. The present investigation revealed that two red color genotypes had an excellent source of antioxidants that demand detail pharmacological study.

Computational Analysis and Synthesis of Syringic Acid Derivatives as Xanthine Oxidase Inhibitors

2020 ◽  
Vol 16 (5) ◽  
pp. 643-653 ◽  
Author(s):  
Neelam Malik ◽  
Anurag Khatkar ◽  
Priyanka Dhiman
Keyword(s):  
Antioxidant Activity ◽  
Uric Acid ◽  
Molecular Docking ◽  
Xanthine Oxidase ◽  
Kinetic Studies ◽  
Inhibitory Effect ◽  
Syringic Acid ◽  
Antioxidant Potential ◽  
Xanthine Oxidase Inhibitors ◽  
Analysis And Synthesis

Background: Xanthine oxidase (XO; EC 1.17.3.2) has been considered as a potent drug target for the cure and management of pathological conditions prevailing due to high levels of uric acid in the bloodstream. The role of xanthine oxidase has been well established in the generation of hyperuricemia and gout due to its important role in catalytic oxidative hydroxylation of hypoxanthine to xanthine and further catalyses of xanthine to generate uric acid. In this research, syringic acid, a bioactive phenolic acid was explored to determine the capability of itself and its derivatives to inhibit xanthine oxidase. Objective: The study aimed to develop new xanthine oxidase inhibitors from natural constituents along with the antioxidant potential. Methods: In this report, we designed and synthesized syringic acid derivatives hybridized with alcohol and amines to form ester and amide linkage with the help of molecular docking. The synthesized compounds were evaluated for their antioxidant and xanthine oxidase inhibitory potential. Results: Results of the study revealed that SY3 produces very good xanthine oxidase inhibitory activity. All the compounds showed very good antioxidant activity. The enzyme kinetic studies performed on syringic acid derivatives showed a potential inhibitory effect on XO ability in a competitive manner with IC50 value ranging from 07.18μM-15.60μM and SY3 was revealed as the most active derivative. Molecular simulation revealed that new syringic acid derivatives interacted with the amino acid residues SER1080, PHE798, GLN1194, ARG912, GLN 767, ALA1078 and MET1038 positioned inside the binding site of XO. Results of antioxidant activity revealed that all the derivatives showed very good antioxidant potential. Conclusion: Molecular docking proved to be an effective and selective tool in the design of new syringic acid derivatives .This hybridization of two natural constituents could lead to desirable xanthine oxidase inhibitors with improved activity.

Enzymatic coupling of chloroanilines with syringic acid, vanillic acid and protocatechuic acid

1994 ◽  
Vol 26 (6) ◽  
pp. 735-742 ◽  
Author(s):  
Kenji Tatsumi ◽  
Alan Freyer ◽  
Robert D. Minard ◽  
Jean-Marc Bollag
Keyword(s):  
Vanillic Acid ◽  
Protocatechuic Acid ◽  
Syringic Acid

scholarly journals High-Performance Thin-Layer Chromatography Densitometric Method for the Simultaneous Determination of Three Phenolic Acids in Syzygium aromaticum (L.) Merr. & Perry

2008 ◽  
Vol 91 (5) ◽  
pp. 1169-1173 ◽  
Author(s):  
Subha Rastogi ◽  
Madan M Pandey ◽  
Ajay K S Rawat
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Simultaneous Determination ◽  
Caffeic Acid ◽  
Correlation Coefficient ◽  
Phenolic Acids ◽  
High Performance ◽  
Syringic Acid ◽  
Syzygium Aromaticum

Abstract A simple, precise, and rapid high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous determination of 3 phenolic acids, i.e., gallic acid, caffeic acid, and syringic acid, in the dried buds of Syzygium aromaticum, commonly known as clove. HPTLC was performed on silica gel 60F254 plates with tolueneethyl acetateformic acid (8 2 1) mobile phase and densitometric scanning at 280 nm. The method was validated for selectivity, linearity, precision, and repeatability. Instrumental precision coefficient of variation (CV) was 0.88, 0.93, and 0.98% and repeatability of the method (CV) was 0.76, 0.64, and 0.69% for gallic acid, caffeic acid, and syringic acid, respectively. The linear concentration ranges were 4003200 ng/spot with a correlation coefficient of 0.993 for gallic acid, 4403520 ng/spot with a correlation coefficient of 0.994 for caffeic acid, and 4004000 ng/spot with a correlation coefficient of 0.993 for syringic acid. The average recoveries of gallic acid, caffeic acid, and syringic acid were 96.3, 95.7, and 92.4%, respectively. Gallic acid, caffeic acid, and syringic acid were present at levels of 1.58, 0.06, and 0.05% (w/w), respectively, in S. aromaticum. This method is simple, accurate, precise, and economical and can be used for routine quality control.

COUMARINS AND PHENOLIC ACIDS IN THE UREDOSPORES OF WHEAT STEM RUST

1957 ◽  
Vol 3 (6) ◽  
pp. 847-862 ◽  
Author(s):  
C. F. van Sumere ◽  
C. van Sumere-de Preter ◽  
L. C. Vining ◽  
G. A. Ledingham
Keyword(s):  
Ferulic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Stem Rust ◽  
Vanillic Acid ◽  
Protocatechuic Acid ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid ◽  
Wheat Stem Rust ◽  
Stimulation Of

A paper chromatographic method suitable for identification of the small amounts of coumarins and phenolic acids present in the uredospores of wheat stem rust was developed. By the use of the circular technique and a combination of three different solvent systems an adequate separation of all the substances was achieved. A preliminary development of the chromatogram with a solvent in which the test compounds were non-mobile facilitated identification and avoided the need for extensive preliminary fractionation of the extracts.Using this method the following compounds were identified in spore extracts: coumarin, umbelliferone, daphnetin, aesculetin, p-hydroxybenzoic acid, vanillic acid, protocatechuic acid, o-coumaric acid, p-coumaric acid, ferulic acid, and caffeic acid; coumarin, p-hydroxybenzoic acid, vanillic acid, protocatechuic acid, o-coumaric acid, and ferulic acid were also present as glycosides; in addition scopoletin, gallic acid, syringic acid, and sinapic acid were detected after hydrolysis and are assumed to be present only in a bound form.In order to obtain some information about the role of these substances in the physiology of wheat stem rust, uredospores were germinated by being floated en masse on dilute aqueous solutions. Of the compounds tested, indoleacetic acid, coumarin, o-coumaric acid, protocatechuic acid, umbelliferone, and daphnetin gave a marked stimulation of germination at concentrations of 10–200 μg./ml. Caffeic acid, vanillic acid, p-hydroxybenzoic acid, ferulic acid, and ferulic acid β-glucoside had little effect or were strongly inhibitory.The stimulation of germination is attributed to the counteraction of a self-inhibitor released from the spores, and the possible significance of the compounds on the physiology of the rust and the host–parasite relationship is discussed.

scholarly journals Molecular docking analysis of beta-catenin with compounds derived from Lycopersicon esculentum

2020 ◽  
Vol 16 (11) ◽  
pp. 801-806
Author(s):  
Jayaraman Selvaraj ◽  
Keyword(s):  
Colorectal Cancer ◽  
Molecular Docking ◽  
Lycopersicon Esculentum ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Vanillic Acid ◽  
Protocatechuic Acid ◽  
Beta Catenin ◽  
Docking Analysis ◽  
Molecular Docking Analysis

Beta-catenin is linked with colorectal cancer (CRC). Therefore, it is of interest to design and develop novel compounds to combat CRC. Hence, we document compounds (chlorogenic acid, gallic acid, protocatechuic acid, quercetin and vanillic acid) from Lycopersicon esculentum with optimal binding features for further consideration.

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