Biomilling of rod-shaped ZnO nanoparticles: a potential role of Saccharomyces cerevisiae extracellular proteins

Porphyrin dyes have an inherent tendency to aggregate. This leads to a self-quenching phenomenon that hinders electron transfer to the conduction band of semiconductors in dye-sensitized solar cells. Self-quenching adversely affects the efficiency of solar cells. Here, we report the interaction of porphyrin with pristine and acid-functionalized fullerene molecules on the surface of ZnO nanoparticles under chemisorbed conditions. Chemisorption of porphyrin only on ZnO nanoparticles instigates aggregation of the porphyrin molecules. These aggregates can be effectively broken by chemisorbing fullerene molecules on the surface of the ZnO nanoparticles. This is due to self-assembly formation processes because of porphyrin–fullerene interactions. The nanohybrid material, consisting of ZnO nanorods, acid-functionalized porphyrin, and fullerene derivatives, was characterized by UV–visible spectroscopy, fourier transform infrared spectroscopy, fluorescence spectroscopy, and transmission electron microscopy. The material generates better performing dye-sensitized solar cells when compared with those fabricated from porphyrin-based photo-active material.

Download Full-text

Potential role of mitochondrial damage and S9 mixture including metabolic enzymes in ZnO nanoparticles-induced oxidative stress and genotoxicity in Chinese hamster lung (CHL/IU) cells

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2018.07.003 ◽

2018 ◽

Vol 834 ◽

pp. 25-34 ◽

Cited By ~ 2

Author(s):

Hiroyuki Yanagisawa ◽

Yoshiko Seki ◽

Shingo Yogosawa ◽

Shota Takumi ◽

Hidesuke Shimizu ◽

...

Keyword(s):

Oxidative Stress ◽

Zno Nanoparticles ◽

Potential Role ◽

Chinese Hamster ◽

Metabolic Enzymes ◽

Mitochondrial Damage

Download Full-text

Role of ZnO Nanoparticles for improvement of Antibacterial Activity in Food Packaging

Asian Journal of Pharmaceutical Research ◽

10.52711/2231-5691.2021.00024 ◽

2021 ◽

pp. 128-131

Author(s):

Saira Sehar ◽

Amiza Amiza ◽

I. H Khan

Keyword(s):

Antimicrobial Activity ◽

Particle Size ◽

Surface Area ◽

Zno Nanoparticles ◽

Food Packaging ◽

Antimicrobial Agents ◽

Uv Light ◽

Zno Nps ◽

Long Time

Nanotechnology advancement leads to development of antimicrobial agents like ZnO nanoparticles. These nanoparticle have their main applications in food packaging. when these nanoparticles incorporate into the food surface, it will kill all bacterias residing on the surface and food become free of bacteria. In this way, food can be stored for a long time because its shelf life is improved. Antimicrobial activity of ZnO nanoparticles can be improved by increasing surface area, reducing particle size and large concentration of ZnO –NPS. Antimicrobial activity increases by increasing intensity of UV light. As UV light fall on ZnO nanoparticles, it increases ZnO surface area and hence anrtimicrobial activity will be increased. Exact mechanism of Antimicrobial activity is still unknown but some processes have been presented.

Download Full-text

Genetic screen for suppressors of increased silencing in rpd3 mutants in Saccharomyces cerevisiae identifies a potential role for H3K4 methylation

G3 Genes|Genome|Genetics ◽

10.1093/g3journal/jkab309 ◽

2021 ◽

Author(s):

Richard A Kleinschmidt ◽

Laurie M Lyon ◽

Samantha L Smith ◽

Jonah Rittenberry ◽

K Maeve Lawless ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Potential Role ◽

Rna Polymerase Iii ◽

Genetic Screen ◽

Gene Repression ◽

Initial Assessment ◽

H3k4 Methylation ◽

Enzyme Functions ◽

Rule Out

Abstract Several studies have identified the paradoxical phenotype of increased heterochromatic gene silencing at specific loci that results from deletion or mutation of the histone deacetylase (HDAC) gene RPD3. To further understand this phenomenon, we conducted a genetic screen for suppressors of this extended silencing phenotype at the HMR locus in Saccharomyces cerevisiae. Most of the mutations that suppressed extended HMR-silencing in rpd3 mutants without completely abolishing silencing were identified in the histone H3 lysine 4 methylation (H3K4me) pathway, specifically in SET1, BRE1 and BRE2. These second site mutations retained normal HMR silencing, therefore appear to be specific for the rpd3Δ extended silencing phenotype. As an initial assessment of the role of H3K4 methylation in extended silencing, we rule out some of the known mechanisms of Set1p/H3K4me mediated gene repression by HST1, HOS2 and HST3 encoded HDACs. Interestingly, we demonstrate that the RNA Polymerase III complex remains bound and active at the HMR-tDNA in rpd3 mutants despite silencing extending beyond the normal barrier. We discuss these results as they relate to the interplay among different chromatin modifying enzyme functions and the importance of further study of this enigmatic phenomenon.

Download Full-text

Photocatalytic and antibacterial biomimetic ZnO nanoparticles

Analytical Methods ◽

10.1039/c7ay01468f ◽

2017 ◽

Vol 9 (33) ◽

pp. 4776-4782 ◽

Cited By ~ 14

Author(s):

Nishant Kumar ◽

Akhshay Singh Bhadwal ◽

Mayank Garg ◽

Reema Sharma ◽

Suman Singh ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Antibacterial Activity ◽

Photocatalytic Degradation ◽

Zno Nanoparticles ◽

Biomimetic Synthesis ◽

Societal Impact ◽

Zno Nps ◽

E Coli ◽

First Time

Societal impact: For the first time, biomimetic synthesis of crystalline and spherical ZnO nanoparticles from Saccharomyces cerevisiae is reported. Photocatalytic degradation of industrial pollutant 4-nitrophenol (4-NP) via biosynthesized ZnO NPs is shown. The antibacterial activity of biosynthesized ZnO NPs against E. coli 1302 is demonstrated.

Download Full-text

CuO and ZnO nanoparticles drive the propagation of antibiotic resistance genes during sludge anaerobic digestion: possible role of stimulated signal transduction

Environmental Science Nano ◽

10.1039/c8en00370j ◽

2019 ◽

Vol 6 (2) ◽

pp. 528-539 ◽

Cited By ~ 16

Author(s):

Haining Huang ◽

Yinguang Chen ◽

Shouye Yang ◽

Xiong Zheng

Keyword(s):

Signal Transduction ◽

Antibiotic Resistance ◽

Anaerobic Digestion ◽

Resistance Genes ◽

Zno Nanoparticles ◽

Antibiotic Resistance Genes ◽

Zno Nps

CuO and ZnO NPs benefit ARG propagation by stimulating signal transduction in sludge bacteria.

Download Full-text

Tryptophan Derivatives by Saccharomyces cerevisiae EC1118: Evaluation, Optimization, and Production in a Soybean-Based Medium

International Journal of Molecular Sciences ◽

10.3390/ijms22010472 ◽

2021 ◽

Vol 22 (1) ◽

pp. 472

Author(s):

Michele Dei Cas ◽

Ileana Vigentini ◽

Sara Vitalini ◽

Antonella Laganaro ◽

Marcello Iriti ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Kynurenic Acid ◽

Potential Role ◽

Defined Medium ◽

The Other ◽

Chemically Defined Medium ◽

Cultural Medium ◽

Whole Cell Biotransformation ◽

Tryptophan Derivatives

Given the pharmacological properti es and the potential role of kynurenic acid (KYNA) in human physiology and the pleiotropic activity of the neurohormone melatonin (MEL) involved in physiological and immunological functions and as regulator of antioxidant enzymes, this study aimed at evaluating the capability of Saccharomyces cerevisiae EC1118 to release tryptophan derivatives (dTRPs) from the kynurenine (KYN) and melatonin pathways. The setting up of the spectroscopic and chromatographic conditions for the quantification of the dTRPs in LC-MS/MS system, the optimization of dTRPs’ production in fermentative and whole-cell biotransformation approaches and the production of dTRPs in a soybean-based cultural medium naturally enriched in tryptophan, as a case of study, were included in the experimental plan. Variable amounts of dTRPs, with a prevalence of metabolites of the KYN pathway, were detected. The LC-MS/MS analysis showed that the compound synthesized at highest concentration is KYNA that reached 9.146 ± 0.585 mg/L in fermentation trials in a chemically defined medium at 400 mg/L TRP. Further experiments in a soybean-based medium confirm KYNA as the main dTRPs, whereas the other dTRPs reached very lower concentrations. While detectable quantities of melatonin were never observed, two MEL isomers were successfully measured in laboratory media.

Download Full-text

POPDC proteins and cardiac function

Biochemical Society Transactions ◽

10.1042/bst20190249 ◽

2019 ◽

Vol 47 (5) ◽

pp. 1393-1404 ◽

Cited By ~ 4

Author(s):

Thomas Brand

Keyword(s):

Potential Role ◽

Striated Muscle ◽

Short Review ◽

Effector Proteins ◽

Muscle Disease ◽

Disease Genes ◽

Protein Protein Interaction ◽

Interaction Partners ◽

And Function

Abstract The Popeye domain-containing gene family encodes a novel class of cAMP effector proteins in striated muscle tissue. In this short review, we first introduce the protein family and discuss their structure and function with an emphasis on their role in cyclic AMP signalling. Another focus of this review is the recently discovered role of POPDC genes as striated muscle disease genes, which have been associated with cardiac arrhythmia and muscular dystrophy. The pathological phenotypes observed in patients will be compared with phenotypes present in null and knockin mutations in zebrafish and mouse. A number of protein–protein interaction partners have been discovered and the potential role of POPDC proteins to control the subcellular localization and function of these interacting proteins will be discussed. Finally, we outline several areas, where research is urgently needed.

Download Full-text