The interaction of QDs with RAW264.7 cells: nanoparticle quantification, uptake kinetics and immune responses study

Exposure to small QDs in high concentration in continuous cell culture results in cell death by apoptosis and necrosis co-existing within the same cell population.

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STING Signaling Promotes Apoptosis, Necrosis, and Cell Death: An Overview and Update

Mediators of Inflammation ◽

10.1155/2018/1202797 ◽

2018 ◽

Vol 2018 ◽

pp. 1-4 ◽

Cited By ~ 5

Author(s):

Song Liu ◽

Wenxian Guan

Keyword(s):

Cell Death ◽

Immune Responses ◽

Tumor Immunity ◽

Host Defense ◽

Cell Types ◽

Immune Homeostasis ◽

Induce Cell Death ◽

Distinct Cell ◽

Clinical Strategies ◽

Apoptosis And Necrosis

STING is a newly identified intracellular sensor of foreign and endogenous DNA. STING has been recognized as an activator of immune responses by TBK1/IRF3 and NF-κB pathways, and it is suggested to play critical roles in host defense, autoimmune diseases, and tumor immunity. Recent studies have revealed that the outcome of STING activation could vary between distinct cell types and scenarios. STING activation in certain cell types triggered cell death including apoptosis and necrosis. This effect could be critical for preventing unnecessary or excessive inflammatory events and maintaining host immune homeostasis. This review is dedicated to summarize recent evidences in the field of STING-mediated cell death and to demonstrate dual outcomes of STING signaling. Besides canonical immune responses represented by IFN and TNF productions, STING signaling can also induce cell death events in a variety of cell types. The double-faced characteristics of STING signaling requires further exploration and precious regulation before tailoring clinical strategies for associated diseases.

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Faculty Opinions recommendation of Promotion of B cell immune responses via an alum-induced myeloid cell population.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1019734.222544 ◽

2004 ◽

Author(s):

Richard Locksley

Keyword(s):

Immune Responses ◽

B Cell ◽

Cell Population ◽

Myeloid Cell

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Combination of Niraparib, Cisplatin and Twist Knockdown in Cisplatin-Resistant Ovarian Cancer Cells Potentially Enhances Synthetic Lethality through ER-Stress Mediated Mitochondrial Apoptosis Pathway

International Journal of Molecular Sciences ◽

10.3390/ijms22083916 ◽

2021 ◽

Vol 22 (8) ◽

pp. 3916

Author(s):

Entaz Bahar ◽

Ji-Ye Kim ◽

Dong-Chul Kim ◽

Hyun-Soo Kim ◽

Hyonok Yoon

Keyword(s):

Ovarian Cancer ◽

Cell Culture ◽

Cell Death ◽

Er Stress ◽

Cisplatin Resistance ◽

Cross Resistance ◽

Ovarian Cancer Cells ◽

Apoptosis Pathway ◽

Platinum Based Chemotherapy

Poly (ADP-ribose) polymerase 1 inhibitors (PARPi) are used to treat recurrent ovarian cancer (OC) patients due to greater survival benefits and minimal side effects, especially in those patients with complete or partial response to platinum-based chemotherapy. However, acquired resistance of platinum-based chemotherapy leads to the limited efficacy of PARPi monotherapy in most patients. Twist is recognized as a possible oncogene and contributes to acquired cisplatin resistance in OC cells. In this study, we show how Twist knockdown cisplatin-resistant (CisR) OC cells blocked DNA damage response (DDR) to sensitize these cells to a concurrent treatment of cisplatin as a platinum-based chemotherapy agent and niraparib as a PARPi on in vitro two-dimensional (2D) and three-dimensional (3D) cell culture. To investigate the lethality of PARPi and cisplatin on Twist knockdown CisR OC cells, two CisR cell lines (OV90 and SKOV3) were established using step-wise dose escalation method. In addition, in vitro 3D spheroidal cell model was generated using modified hanging drop and hydrogel scaffolds techniques on poly-2-hydroxylethly methacrylate (poly-HEMA) coated plates. Twist expression was strongly correlated with the expression of DDR proteins, PARP1 and XRCC1 and overexpression of both proteins was associated with cisplatin resistance in OC cells. Moreover, combination of cisplatin (Cis) and niraparib (Nira) produced lethality on Twist-knockdown CisR OC cells, according to combination index (CI). We found that Cis alone, Nira alone, or a combination of Cis+Nira therapy increased cell death by suppressing DDR proteins in 2D monolayer cell culture. Notably, the combination of Nira and Cis was considerably effective against 3D-cultures of Twist knockdown CisR OC cells in which Endoplasmic reticulum (ER) stress is upregulated, leading to initiation of mitochondrial-mediated cell death. In addition, immunohistochemically, Cis alone, Nira alone or Cis+Nira showed lower ki-67 (cell proliferative marker) expression and higher cleaved caspase-3 (apoptotic marker) immuno-reactivity. Hence, lethality of PARPi with the combination of Cis on Twist knockdown CisR OC cells may provide an effective way to expand the therapeutic potential to overcome platinum-based chemotherapy resistance and PARPi cross resistance in OC.

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Overexpression of Transcripts Coding for Renin-b but Not for Renin-a Reduce Oxidative Stress and Increase Cardiomyoblast Survival under Starvation Conditions

Cells ◽

10.3390/cells10051204 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1204

Author(s):

Heike Wanka ◽

Philipp Lutze ◽

Alexander Albers ◽

Janine Golchert ◽

Doreen Staar ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Death ◽

B Cells ◽

Apoptotic Cell ◽

Renin Angiotensin System ◽

Glucose Deprivation ◽

Protective Effects ◽

Glucose Depletion ◽

A Cells ◽

Apoptosis And Necrosis

A stimulated renin-angiotensin system is known to promote oxidative stress, apoptosis, necrosis and fibrosis. Renin transcripts (renin-b; renin-c) encoding a cytosolic renin isoform have been discovered that may in contrast to the commonly known secretory renin (renin-a) exert protective effects Here, we analyzed the effect of renin-a and renin-b overexpression in H9c2 cardiomyoblasts on apoptosis and necrosis as well as on potential mechanisms involved in cell death processes. To mimic ischemic conditions, cells were exposed to glucose starvation, anoxia or combined oxygen–glucose deprivation (OGD) for 24 h. Under OGD, control cells exhibited markedly increased necrotic and apoptotic cell death accompanied by enhanced ROS accumulation, loss of mitochondrial membrane potential and decreased ATP levels. The effects of OGD on necrosis were exaggerated in renin-a cells, but markedly diminished in renin-b cells. However, with respect to apoptosis, the effects of OGD were almost completely abolished in renin-b cells but interestingly also moderately diminished in renin-a cells. Under glucose depletion we found opposing responses between renin-a and renin-b cells; while the rate of necrosis and apoptosis was aggravated in renin-a cells, it was attenuated in renin-b cells. Based on our results, strategies targeting the regulation of cytosolic renin-b as well as the identification of pathways involved in the protective effects of renin-b may be helpful to improve the treatment of ischemia-relevant diseases.

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Biomarkers of Radiotherapy-Induced Immunogenic Cell Death

Cells ◽

10.3390/cells10040930 ◽

2021 ◽

Vol 10 (4) ◽

pp. 930

Author(s):

Rianne D. W. Vaes ◽

Lizza E. L. Hendriks ◽

Marc Vooijs ◽

Dirk De Ruysscher

Keyword(s):

Cell Death ◽

Immune Responses ◽

Tumor Cells ◽

Immunogenic Cell Death ◽

Type I ◽

Future Studies ◽

Regulated Cell Death ◽

Molecular Patterns ◽

Damage Associated Molecular Patterns ◽

Potential Biomarkers

Radiation therapy (RT) can induce an immunogenic variant of regulated cell death that can initiate clinically relevant tumor-targeting immune responses. Immunogenic cell death (ICD) is accompanied by the exposure and release of damage-associated molecular patterns (DAMPs), chemokine release, and stimulation of type I interferon (IFN-I) responses. In recent years, intensive research has unraveled major mechanistic aspects of RT-induced ICD and has resulted in the identification of immunogenic factors that are released by irradiated tumor cells. However, so far, only a limited number of studies have searched for potential biomarkers that can be used to predict if irradiated tumor cells undergo ICD that can elicit an effective immunogenic anti-tumor response. In this article, we summarize the available literature on potential biomarkers of RT-induced ICD that have been evaluated in cancer patients. Additionally, we discuss the clinical relevance of these findings and important aspects that should be considered in future studies.

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Combining PD-L1 Inhibitors with Immunogenic Cell Death Triggered by Chemo-Photothermal therapy via a Thermosensitive Liposome System to Stimulate Tumor-specific Immunological Response

Nanoscale ◽

10.1039/d1nr03288g ◽

2021 ◽

Author(s):

Jie Yu ◽

Xidong He ◽

Zigui Wang ◽

Yu Peng Wang ◽

Sha Liu ◽

...

Keyword(s):

Cell Death ◽

Immune Responses ◽

Photothermal Therapy ◽

Immunological Response ◽

Immune Checkpoint Blockade ◽

Immunogenic Cell Death ◽

Adaptive Immune Responses ◽

Adaptive Immune ◽

Thermosensitive Liposome ◽

Liposome System

Immune checkpoint blockade (ICB) therapy in combination with immunogenic death (ICD) triggered by photothermal therapy (PTT) and oxaliplatin (OXA) treatment was expected to elicit both innate and adaptive immune responses...

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Inhibition of B-cell death does not restore T-cell-dependent immune responses in CD40-deficient mice

Immunology ◽

10.1046/j.1365-2567.2003.01690.x ◽

2003 ◽

Vol 109 (4) ◽

pp. 504-509

Author(s):

Jesus Merino ◽

Miguel A. Diez ◽

Maria Muniz ◽

Luis Buelta ◽

Gabriel Nunez ◽

...

Keyword(s):

Cell Death ◽

T Cell ◽

Immune Responses ◽

B Cell ◽

Deficient Mice

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Cell death in cell culture frame by frame: Spontaneous cell demise of a 3T3 fibroblast

Journal of Cellular and Molecular Medicine ◽

10.1111/j.1582-4934.2003.tb00219.x ◽

2003 ◽

Vol 7 (2) ◽

pp. 192-193 ◽

Cited By ~ 1

Author(s):

M. Leabu ◽

M. E. Hinescu ◽

Shashi Uniyal ◽

B. M. C. Chan

Keyword(s):

Cell Culture ◽

Cell Death

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Tumor Microenvironment-Triggered In-Situ Cancer Vaccines with Dual Immunogenic Cell Death Inductions for Elevated Antitumor and Antimetastatic Therapy

Nanoscale ◽

10.1039/d1nr02018h ◽

2021 ◽

Author(s):

Jun Lin ◽

Binbin Ding ◽

Pan Zheng ◽

Dong Li ◽

Meifang Wang ◽

...

Keyword(s):

Cell Death ◽

Tumor Microenvironment ◽

Immune Responses ◽

Cancer Immunotherapy ◽

Cancer Vaccines ◽

High Specificity ◽

The Body ◽

Immunogenic Cell Death ◽

Specific Antigens

Cancer vaccine is to make tumor-specific antigens into vaccines, which then are injected back into the body to activate immune responses for cancer immunotherapy. Despite the high specificity and therapeutic...

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