scholarly journals More from less: high-throughput dual polarity lipid imaging of biological tissues

The Analyst ◽  
2016 ◽  
Vol 141 (12) ◽  
pp. 3832-3841 ◽  
Author(s):  
Shane R. Ellis ◽  
Joanna Cappell ◽  
Nina Ogrinc Potočnik ◽  
Benjamin Balluff ◽  
Julie Hamaide ◽  
...  
Keyword(s):  
High Throughput ◽  
High Speed ◽  
Spatial Information ◽  
Biological Tissues ◽  
Tissue Sections ◽  
Dual Polarity

Here, we reveal the increased biochemical and spatial information acquired using high-speed MALDI-MSI and sequential acquisitions of positive and negative lipid-MSI data from single tissue sections.

scholarly journals Imaging biological tissue with high-throughput single-pixel compressive holography

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Daixuan Wu ◽  
Jiawei Luo ◽  
Guoqiang Huang ◽  
Yuanhua Feng ◽  
Xiaohua Feng ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Spatial Information ◽  
Biological Tissues ◽  
Large Field ◽  
Pixel Detector ◽  
Array Detectors ◽  
Pixel Array ◽  
Compressive Holography ◽  
Single Pixel

AbstractSingle-pixel holography (SPH) is capable of generating holographic images with rich spatial information by employing only a single-pixel detector. Thanks to the relatively low dark-noise production, high sensitivity, large bandwidth, and cheap price of single-pixel detectors in comparison to pixel-array detectors, SPH is becoming an attractive imaging modality at wavelengths where pixel-array detectors are not available or prohibitively expensive. In this work, we develop a high-throughput single-pixel compressive holography with a space-bandwidth-time product (SBP-T) of 41,667 pixels/s, realized by enabling phase stepping naturally in time and abandoning the need for phase-encoded illumination. This holographic system is scalable to provide either a large field of view (~83 mm2) or a high resolution (5.80 μm × 4.31 μm). In particular, high-resolution holographic images of biological tissues are presented, exhibiting rich contrast in both amplitude and phase. This work is an important step towards multi-spectrum imaging using a single-pixel detector in biophotonics.

scholarly journals Fully Automated Cultivation of Adipose-Derived Stem Cells in the StemCellDiscovery—A Robotic Laboratory for Small-Scale, High-Throughput Cell Production Including Deep Learning-Based Confluence Estimation

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 575
Author(s):  
Jelena Ochs ◽  
Ferdinand Biermann ◽  
Tobias Piotrowski ◽  
Frederik Erkens ◽  
Bastian Nießing ◽  
...  
Keyword(s):  
Stem Cells ◽  
Deep Learning ◽  
High Throughput ◽  
High Speed ◽  
New Technologies ◽  
Human Mesenchymal Stem Cells ◽  
Simulation Software ◽  
System Capacity ◽  
Small Scale ◽  
Production Environment

Laboratory automation is a key driver in biotechnology and an enabler for powerful new technologies and applications. In particular, in the field of personalized therapies, automation in research and production is a prerequisite for achieving cost efficiency and broad availability of tailored treatments. For this reason, we present the StemCellDiscovery, a fully automated robotic laboratory for the cultivation of human mesenchymal stem cells (hMSCs) in small scale and in parallel. While the system can handle different kinds of adherent cells, here, we focus on the cultivation of adipose-derived hMSCs. The StemCellDiscovery provides an in-line visual quality control for automated confluence estimation, which is realized by combining high-speed microscopy with deep learning-based image processing. We demonstrate the feasibility of the algorithm to detect hMSCs in culture at different densities and calculate confluences based on the resulting image. Furthermore, we show that the StemCellDiscovery is capable of expanding adipose-derived hMSCs in a fully automated manner using the confluence estimation algorithm. In order to estimate the system capacity under high-throughput conditions, we modeled the production environment in a simulation software. The simulations of the production process indicate that the robotic laboratory is capable of handling more than 95 cell culture plates per day.

High-speed and high-precision fluorescence-based cell count and viability assays using the Cellaca™ MX high-throughput cell counter

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S97
Author(s):  
J. Bell ◽  
Y. Huang ◽  
S. Yung ◽  
H. Qazi ◽  
C. Hernandez ◽  
...  
Keyword(s):  
Cell Count ◽  
High Precision ◽  
High Throughput ◽  
High Speed ◽  
Cell Counter

High Speed FPGA Based 128-bit Advance Encryption Standard (AES)

2021 ◽  
Vol 11 ◽  
Author(s):  
Ibrahem M. T. Hamidi ◽  
Farah S. H. Al-aassi
Keyword(s):  
High Throughput ◽  
Field Programmable Gate Array ◽  
High Speed ◽  
Design Tool ◽  
Advanced Encryption Standard ◽  
Clock Frequency ◽  
Advance Encryption Standard ◽  
Field Programmable ◽  
Internal Component ◽  
Gate Array

Aim: Achieve high throughput 128 bits FPGA based Advanced Encryption Standard. Background: Field Programmable Gate Array (FPGA) provides an efficient platform for design AES cryptography system. It provides the capability to control over each bit using HDL programming language such as VHDL and Verilog which results an output speed in Gbps rang. Objective: Use Field Programmable Gate Array (FPGA) to design high throughput 128 bits FPGA based Advanced Encryption Standard. Method: Pipelining technique has used to achieve maximum possible speed. The level of pipelining includes round pipelining and internal component pipelining where number of registers inserted in particular places to increase the output speed. The proposed design uses combinatorial logic to implement the byte substitution. The s-box implemented using composed field arithmetic with 7 stages of pipelining to reduce the combinatorial logic level. The presented model has implemented using VHDL in Xilinix ISETM 14.4 design tool. Result: The achieved results were 18.55 Gbps at a clock frequency of 144.96 MHz and area of 1568 Slices in Spartan3 xc3s1000 hardware. Conclusion: The results show that the proposed design reaches a high throughput with acceptable area usage compare with other designs in the literature.

scholarly journals Achieving High-Speed Retraction in a Stretchable Hydrogel

2020 ◽  
Author(s):  
Rosa Maria Badani Prado ◽  
Satish Mishra ◽  
Buckston Morgan ◽  
Rangana Wijayapala ◽  
Seyed Meysam Hashemnejad ◽  
...  
Keyword(s):  
High Speed ◽  
Biological Tissues ◽  
Biological Species ◽  
Vital Role ◽  
Polypropylene Glycol ◽  
Glycol Diacrylate ◽  
Mantis Shrimp ◽  
Power Amplification ◽  
Short Period ◽  
Very High

Many biological species apply the power amplification mechanism for locomotion, feeding, and protection. In power amplification, a biological system rapidly releases stored-energy by achieving a very high velocity over a short period of time, resulting in high power output. Such power amplification allows insects such as locust to jump and Mantis shrimp to kill prey by its appendage strike. Biological elastomeric polymers such as resilin play a vital role in the power amplification process because of their high stretchability and resilience. In synthetic materials, although<br>crosslinked rubbers display high stretchability and resilience, such is difficult to achieve in the water-containing systems such as in hydrogels, commonly considered materials for mimicking biological tissues. Here, we have used a simple free-radical polymerization of acrylic acid (AAc), methacrylamide (MAAm), and polypropylene glycol diacrylate (PPGDA) to obtain hydrogels. In these gels, the polymerized AAc and MAAm act as hydrophilic blocks and PPG as hydrophobic, and the gel structure resemble that of resilin consisting of hydrophilic and hydrophobic components. The bioinspired gels display very high stretchability, as high as eight times the original length, and greater than 90% resilience. In addition, the gel samples can reach a retraction velocity of 16 m/s with an acceleration of 4X10^3 m/s2. These values are similar or better than those observed in water containing biological systems, such as appendage strikes in Mantis shrimp, etc. To the best of our knowledge, such performance has not been reported in the<br>literature for any water containing networks.

Spatial-Prior-Guided Attention for Small Object Detection in Overhead Catenary System

2021 ◽  
Author(s):  
Hang Gong ◽  
Shangdong Zheng ◽  
Zebin Wu ◽  
Yang Xu ◽  
Zhihui Wei ◽  
...  
Keyword(s):  
Object Detection ◽  
Network Architecture ◽  
High Speed ◽  
Spatial Information ◽  
Spatial Relations ◽  
Economic Loss ◽  
Small Object ◽  
Feed Forward Network ◽  
Catenary System ◽  
Small Object Detection

The small defects in overhead catenary system (OCS) can result in long time delays, economic loss and even passenger injury. However, OCS images exhibit great variations with complex background and oblique views which pose a great challenge for small defects detection in high-speed rail system. In this paper, we propose the spatial-prior-guided attention for small object detection in OCS with two main advantages: (1) The spatial-prior is proposed to retain the spatial information between small defects and the electric components in OCS. (2) Based on spatial-prior, the spatial-prior-guided attention model (SAM) is designed to highlight useful information in the features and suppress redundant features response. SAM can model the spatial relations progressively and can be integrated with state-of-the-art feed-forward network architecture with end-to-end training fashion. We conduct extensive experiments on both Split pin datasets and PASCAL–VOC datasets and achieve 97.2% and 79.5% mAP values, respectively. All the experiments demonstrate the competitive performance of our method.

Enhanced Air-Gap Control for High-Speed Plasmonic Lithography Using Solid Immersion Lens With Sharp-Ridge Nanoaperture

2011 ◽  
Author(s):  
Hyunwoo Hwang ◽  
Won-Sup Lee ◽  
No-Cheol Park ◽  
Hyunseok Yang ◽  
Young-Pil Park ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
High Speed ◽  
Low Cost ◽  
Near Field ◽  
Nanometer Scale ◽  
Field Region ◽  
High Speeds ◽  
Gap Control

Recently, plasmonic nanolithography is studied by many researchers (1, 2 and 3). This presented a low-cost and high-throughput approach to maskless nanolithography technique that uses a metallic sharp-ridge nanoaperture with a high strong nanometer-sized optical spot induced by surface plasmon resonance. However, these nanometer-scale spots generated by metallic nanoapertures are formed in only the near-field region, which makes it very difficult to pattern above the photoresist surface at high-speeds.

scholarly journals High Throughput Error Control Using Parallel CRC

VLSI Design ◽  
1994 ◽  
Vol 2 (1) ◽  
pp. 33-50
Author(s):  
Andrzej Sobski ◽  
Alexander Albicki
Keyword(s):  
High Throughput ◽  
High Speed ◽  
Error Control ◽  
Cyclic Codes ◽  
Shift Register ◽  
Linear Feedback ◽  
Clock Period ◽  
Feedback Shift Register ◽  
Parallel Stream ◽  
Speed Computer

Redesigning the LFSR (Linear Feedback Shift Register) so that syndrome calculations can be performed in one sweep allows for fast error control in high speed computer networks. The resulting structure forms the basis of the PEDDC (Parallel Encoder, Decoder, Detector, Corrector) which replaces the conventional Serial Encoder, Decoder, Detector, Corrector for generation and utilization of cyclic codes. Since syndromes are calculated in as little as one clock period, information from which the syndrome is calculated can be processed in a parallel stream. In this paper a simple PEDDC is built, its operation is examined in detail, its performance is compared with a serial counterpart, possible variations on the PEDDC structure is given, and further speed enhancement techniques are considered.

High-Throughput DNA Plasmid Transfection Using Acoustic Droplet Ejection Technology

2018 ◽  
Vol 24 (4) ◽  
pp. 492-500
Author(s):  
Béatrice Colin ◽  
Benoit Deprez ◽  
Cyril Couturier
Keyword(s):  
High Throughput ◽  
Plasmid Dna ◽  
High Speed ◽  
Protein Crystallization ◽  
Dna Transfection ◽  
Dna Amount ◽  
Biological Assays ◽  
Droplet Ejection ◽  
Plasmid Transfection ◽  
Automated Protocol

The Labcyte Echo acoustic liquid handler allows accurate droplet ejection at high speed from a source well plate to a destination plate. It has already been used in various miniaturized biological assays, such as quantitative PCR (q-PCR), quantitative real-time PCR (q-RT-PCR), protein crystallization, drug screening, cell dispensing, and siRNA transfection. However, no plasmid DNA transfection assay has been published so far using this dispensing technology. In this study, we evaluated the ability of the Echo 550 device to perform plasmid DNA transfection in 384-well plates. Due to the high throughput of this device, we simultaneously optimized the three main parameters of a transfection process: dilution of the transfection reagent, DNA amount, and starting DNA concentration. We defined a four-step protocol whose optimal settings allowed us to transfect HeLa cells with up to 90% efficiency and reach a co-expression of nearly 100% within transfected cells in co-transfection experiments. This fast, reliable, and automated protocol opens new ways to easily and rapidly identify optimal transfection settings for a given cell type. Furthermore, it permits easy software-based transfection control and multiplexing of plasmids distributed on wells of a source plate. This new development could lead to new array applications, such as human ORFeome protein expression or CRISPR-Cas9-based gene function validation in nonpooled screening strategies.

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