Synergistic antibacterial activity of a combination of silver and copper nanoparticle impregnated activated carbon for water disinfection

This research aimed to enhance the antibacterial activity of silver nanoparticles (AgNPs) synthesized from silver nitrate (AgNO3) using aloe vera extract. It was performed by means of incorporating AgNPs on an activated carbon nanoparticle (ACNPs) under ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min in an aqueous colloidal medium. The successful AgNPs synthesis was clarified with both Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FTIR) spectrophotometers. The successful AgNPs–ACNPs incorporation and its particle size analysis was performed using Transmission Electron Microscope (TEM). The brown color suspension generation and UV-Vis’s spectra maximum wavelength at around 480 nm confirmed the existence of AgNPs. The particle sizes of the produced AgNPs were about 5 to 10 nm in the majority number, which collectively surrounded the aloe vera extract secondary metabolites formed core-shell like nanostructure of 8.20 ± 2.05 nm in average size, while ACNPs themselves were about 20.10 ± 1.52 nm in average size formed particles cluster, and 48.00 ± 8.37 nm in average size as stacking of other particles. The antibacterial activity of the synthesized AgNPs and AgNPs-immobilized ACNPs was 57.58% and 63.64%, respectively (for E. coli); 61.25%, and 93.49%, respectively (for S. aureus). In addition, when the AgNPs-immobilized ACNPs material was coated on the cotton and polyester fabrics, the antibacterial activity of the materials changed, becoming 19.23% (cotton; E. coli), 31.73% (polyester; E. coli), 13.36% (cotton; S. aureus), 21.15% (polyester; S. aureus).

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Temperature Dependent On Activated Carbon For Their Biological Activity And Photocatalytical Degradation of Methylene Blue: A Novel Approach To Green Synthesis

10.21203/rs.3.rs-792713/v1 ◽

2021 ◽

Author(s):

Amalanathan.M ◽

Aravind.M ◽

Sony Michael Mary.M ◽

Razan A. Alshgari ◽

Asma A. Alothman ◽

...

Keyword(s):

Methylene Blue ◽

Antibacterial Activity ◽

Activated Carbon ◽

Hydrothermal Carbonization ◽

Microbial Pathogens ◽

X Ray Diffraction ◽

E Coli ◽

Novel Approach ◽

Transmission Electron ◽

Ft Ir

Abstract In this work, jasmine flower derived activated carbon were successfully synthesized by hydrothermal carbonization process at the different annealing temperature. The Crystallinity, phase, structural, morphological and optical properties of activated carbon were investigated using X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), Transmission electron microscope (TEM), and UV-visible spectroscopy analysis. The graphitic phase of carbon was obtained from the XRD pattern. Surface morphology reveals irregular-shaped nanoparticles. The photodegradation of methylene blue (MB) was carried out under the visible light irradiation technique to study its photocatalytic activity. The activated carbon obtained at 400oC, 500oC and 600oC shows a photocatalytic degradation efficiency of 86%, 90%, and 94%, respectively. Antibacterial activity of activated carbon was examined against S. Aureus (MTCC-737) and E-Coli (MTCC- 443) microbial pathogens, and their potent antibacterial activity was examined from the zone of inhibition layer.

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Influence of pyrolytic seeds on ZnO nanorod growth onto rigid substrates for photocatalytic abatement of Escherichia coli in water

Water Science & Technology Water Supply ◽

10.2166/ws.2014.068 ◽

2014 ◽

Vol 14 (6) ◽

pp. 1087-1094 ◽

Cited By ~ 2

Author(s):

Luis Sanchez ◽

Lucas Guz ◽

Pilar García ◽

Silvia Ponce ◽

Silvia Goyanes ◽

...

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Zno Nanorods ◽

Water Disinfection ◽

Acetate Solution ◽

Ethanol Mixture ◽

E Coli ◽

The Relationship ◽

Nanorod Growth

ZnO nanorods (ZnO NRs) were grown on ZnO seeded fluorine doped tin oxide (FTO) substrates at low temperatures (90 °C) from Zn2+ precursors in alkaline aqueous solution. The ZnO seeds were deposited on the FTO substrate heated at 350 °C by spray pyrolysis of a zinc acetate solution in a water ethanol mixture. The structure of seeds was tuned by the ethanol water ratio, Γ, which controls the solvent evaporation rate of drops impinging the substrate. The relationship between the microstructure and optical properties of the ZnO NR films and the photocatalytic antibacterial activity for Escherichia coli abatement, was determined through a detailed characterization of the material. The higher photocatalytic antibacterial activity was performed by ZnO NR films grown on seeds deposited from solutions with Γ in the 0.0–0.03 range. With these films, the population of viable E. coli dropped more than six orders, from 8 × 108 to 4 × 102 CFU. These results show the potential of these materials in water disinfection.

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Antibacterial properties of Ag and Ag/AgCl nanoparticles from radish and tea extracts for water treatment applications

Water Science & Technology Water Supply ◽

10.2166/ws.2015.124 ◽

2015 ◽

Vol 16 (1) ◽

pp. 171-179

Author(s):

Yuphada Boonto ◽

Jirapat Ananpattarachai ◽

Puangrat Kajitvichyanukul

Keyword(s):

Antibacterial Activity ◽

Water Treatment ◽

Microwave Irradiation ◽

Green Synthesis ◽

Plant Extracts ◽

Antibacterial Properties ◽

Water Disinfection ◽

E Coli ◽

High Antibacterial Activity ◽

Complete Inactivation

Silver nanoparticles (AgNPs) have antibacterial properties and are widely used for water disinfection. This technology is commercially applied in point-of-use water treatment as a post-treatment for filtrate water. However, the current process of synthesizing AgNPs has several disadvantages including the use of hazardous chemicals, consumption of a large amount of energy and the formation of hazardous byproducts. Here, we report an alternative and green synthesis using plant extracts. In this work, the plant extracts came from radish (R) and tea (T), and the AgNPs were derived from a microwave irradiation method. The AgNPs synthesized by chemical-based microwave irradiation (Ag-C) were also used as a control material. The novel method produced a smaller size of nanostructures with good dispersion ability and less agglomeration than those from chemical synthesis. The antibacterial properties of AgNPs on Gram-negative bacteria Escherichia coli (E. coli) and Gram-positive bacteria Staphylococcus aureus (S. aureus) were investigated. The results revealed that AgNPs from both green synthesis and chemical-based methods inactivated both types of bacteria. The green-synthesized AgNPs from radish juice provided a higher percentage of inhibition of E. coli than that of S. aureus. The inactivation rates of the AgNPs increased with increasing concentration of AgNPs. As the concentration of the Ag/AgCl-R and Ag-R increased from 150 μg/mL to 300 μg/mL, complete inactivation required a reduced time for the reaction from 300 minutes to only 30 minutes. Finally, the Ag/AgCl-R and Ag-R offered high antibacterial activity while the Ag-T provided the lowest antibacterial activity. This work provides an alternative method for the eco-synthesis of antibacterial nanomaterials for water treatment.

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Electrosorptive disinfection of Escherichia coli (E. coli) aqueous solutions by activated carbon monolith electrodes

Water Science & Technology Water Supply ◽

10.2166/ws.2020.282 ◽

2020 ◽

Author(s):

Edip Bayram ◽

Ceren Karaman ◽

Zehra Kuru ◽

Onur Karaman

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Activated Carbon ◽

Aqueous Solutions ◽

Industrial Process ◽

E Coli ◽

Carbon Monolith ◽

As Adsorption ◽

Activated Carbon Monolith ◽

Better Than

Abstract Electrosorption, which can be defined as adsorption onto the surfaces of charged electrodes, has been developing as an efficient and environmentally friendly technology for removing toxic pollutants from aqueous solutions. In this study, an industrial process was used for the fabrication of activated carbon electrodes (ACEs). An electroless metal deposition method was used for the modification of activated carbon granules with silver (Ag) for antibacterial activity of electrodes. The antibacterial activity of Ag-modified–ACEs (Ag–ACEs) for Escherichia coli (E. coli) bacteria commonly found in water was tested. Adsorption and electrosorption behaviors of E. coli aqueous solutions onto ACEs and Ag–ACEs were examined in a cyclic electrosorption system. It has been concluded that the performance of Ag–ACEs is better than ACEs as an electrode for electrosorption of E. coli. Moreover polarization can significantly enhance the removal efficiency of E. coli on both ACEs and Ag–ACEs. Finally, electrosorption capacity of the system for E. coli was determined.

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Investigation of surfactant-modified activated carbon for recycled water disinfection

Water Science & Technology ◽

10.2166/wst.2010.458 ◽

2010 ◽

Vol 62 (8) ◽

pp. 1755-1766 ◽

Cited By ~ 4

Author(s):

T. Garoma ◽

J. Kocher

Keyword(s):

Activated Carbon ◽

Contact Time ◽

Total Coliform ◽

Water Disinfection ◽

Hexadecyltrimethylammonium Bromide ◽

Recycled Water ◽

Experimental Conditions ◽

Modified Activated Carbon ◽

E Coli ◽

Didodecyldimethylammonium Bromide

This study investigated the effectiveness of surfactant-loaded granular activated carbon (GAC) to deactivate total coliform, E. coli, and enterococci found in tertiary effluent under various experimental conditions, i.e. varying surfactant dose, GAC dose, and contact time. The results indicate that GAC loaded with 100 mg/g of hexadecyltrimethylammonium bromide (CTAB) and didodecyldimethylammonium bromide (DDAB), achieved log reductions as high as 1.02 and 1.86 of total coliform, respectively. At varying GAC doses and contact times, 200 mg/g of DDAB dose achieved 99.9 to 100% reduction in total coliform at initial concentrations as high as 38,000 MPN/100 mL. Complete deactivation of E. coli and enterococci were observed for CTAB and DDAB at 200 mg/g dose for varying GAC doses and contact times used in this study. DDAB was more effective than CTAB at deactivating total coliform and E. coli, both Gram-negative bacteria, while both surfactants were shown to have similar disinfection capabilities against enterococci. Surfactant dose and GAC dose were shown to enhance bacteria deactivation; however, surfactant dose was found to be the most important parameter. For contact times evaluated in this research, bacterial deactivation remained the same or slightly decreased with contact time. In conclusion, surfactant-modified GAC can be used as an effective disinfection technique for recycled water.

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PERBANDINGAN PENGUJIAN AKTIVITAS ANTIBAKTERI STARTER YOGURT DENGAN METODE DIFUSI SUMURAN DAN METODE DIFUSI CAKRAM

Jurnal Teknologi Hasil Peternakan ◽

10.24198/jthp.v1i2.27537 ◽

2020 ◽

Vol 1 (2) ◽

pp. 41

Author(s):

Lilih Siti Nurhayati ◽

Nadhira Yahdiyani ◽

Akhmad Hidayatulloh

Keyword(s):

Antibacterial Activity ◽

Bacterial Activity ◽

Disk Diffusion ◽

Diffusion Method ◽

Testing Methods ◽

Disk Diffusion Method ◽

E Coli ◽

Agar Diffusion ◽

Agar Diffusion Method ◽

Agar Well Diffusion Method

Antibacterial activity testing can be done using the agar diffusion method, including agar well difussion and disk diffusion agar methods. This study aims to compare two antibacterial testing methods to analyze the anti-bacterial activity of a yogurt starter against the bacteria Eschericia coli and Staphilococcus aureus. The study was conducted experimentally with 5 concentrations of yogurt starter, namely 2%, 4%, 6%, 8%, and 10%. Testing antibacterial activity using two methods ,disk diffusion agar and well difussion agar methods. The research showed that agar well diffusion method obtained antibacterial activity greater than the disk diffusion method for E. coli and S. aureus.

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Disinfection of Escherichia coli by a Reactive Electrochemical Membrane System Involving Activated Carbon Fiber Cloth (ACFC)

Water ◽

10.3390/w11030430 ◽

2019 ◽

Vol 11 (3) ◽

pp. 430

Author(s):

Edward Huang ◽

Townshend White ◽

Beibei Wang ◽

Huanhuan Shi ◽

Jiayang Liu

Keyword(s):

Escherichia Coli ◽

Activated Carbon ◽

Carbon Fiber ◽

Operation Time ◽

Water Disinfection ◽

Activated Carbon Fiber ◽

E Coli ◽

Carbon Fiber Cloth ◽

Unit Reduction ◽

Activated Carbon Fiber Cloth

This study examined a novel reactive electrochemical membrane (REM) system with activated carbon fiber cloth (ACFC) serving simultaneously as the anode and the membrane to effectively disinfect water that was filtered through the device. An Escherichia coli strain was inoculated to water as a model pathogen. The influence of REM operation parameters, including the number of ACFC layers, voltage, flow rate and operation time, was evaluated. Up to 7.5 log unit reduction of E. coli concentration in water was achieved at the optimal treatment condition, while the energy consumption was 1.5 kWh/m3 per log unit reduction of E. coli. This makes it possible to use this ACFC-based REM technology for point-of-use water disinfection to provide clean water for underdeveloped regions. Further tests by free radical probing, Linear Scan Voltammetry (LSV) and Scanning Electron Microscopy (SEM) suggest that the disinfection involved the filtration/retention of bacteria on ACFC and attack by reactive oxygen species generated electrochemically on the anode.

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Synergistic antibacterial activity of surfactant free Ag–GO nanocomposites

Scientific Reports ◽

10.1038/s41598-020-80013-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Muhammad Ashfaq Ahmad ◽

Samia Aslam ◽

Faiza Mustafa ◽

Usman Arshad

Keyword(s):

Antibacterial Activity ◽

Ag Nanoparticles ◽

Antibacterial Agent ◽

Force Microscopy ◽

E Coli ◽

Atomic Force ◽

Xrd Study ◽

One Step ◽

Ftir And Raman Spectroscopy ◽

Synergistic Antibacterial Activity

AbstractGraphene oxide–silver (Ag–GO) nanocomposite has emerged as a vital antibacterial agent very recently. In this work, we report a facile one step route of Ag–GO nanocomposite formation excluding the aid of surfactants and reductants and was successfully applied to negative Escherichia Coli (E coli) to investigate antibacterial activity by varying doze concentration. The successful formation of Ag–GO nanocomposite via facile one step route was confirmed using Fourier transform infrared spectroscopy (FTIR) and Raman Spectroscopy. The absorption spectra (peak ~ 300 nm) for GO and the (peak ~ 420 nm) for silver nanoparticles were observed. XRD study confirmed the formation of Ag–GO nanocomposite while atomic force microscopy (AFM) showed crumbled GO sheets decorated with Ag nanoparticles. It was observed that the functional groups of GO facilitated the binding of Ag nanoparticles to GO network and enhanced the antibacterial activity of the nanocomposite.

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