Bacterial inactivation by a carbon nanotube–iron oxide nanocomposite: a mechanistic study usingE. colimutants

2018 ◽  
Vol 5 (2) ◽  
pp. 372-380 ◽  
Author(s):  
Maya Engel ◽  
Yitzhak Hadar ◽  
Shimshon Belkin ◽  
Xinglin Lu ◽  
Menachem Elimelech ◽  
...  

Inactivation ofE. coliby the carbon nanotube–iron oxide nanocomposite.

Nanomaterials ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 18 ◽  
Author(s):  
Yu Gu ◽  
Furen Xiao ◽  
Liumin Luo ◽  
Xiaoyu Zhou ◽  
Xiaodong Zhou ◽  
...  

Many disinfection technologies have emerged recently in water treatment industry, which are designed to inactivate water pathogens with extraordinary efficiency and minimum side effects and costs. Current disinfection processes, including chlorination, ozonation, UV irradiation, and so on, have their inherent drawbacks, and have been proven ineffective under certain scenarios. Bacterial inactivation by noble metals has been traditionally used, and copper is an ideal candidate as a bactericidal agent owing to its high abundance and low cost. Building on previous findings, we explored the bactericidal efficiency of Cu(I) and attempted to develop it into a novel water disinfection platform. Nanosized copper ferrite was synthesized, and it was reduced by hydroxylamine to form surface bound Cu(I) species. Our results showed that the generated Cu(I) on copper ferrite surface could inactivate E. coli at a much higher efficiency than Cu(II) species. Elevated reactive oxygen species’ content inside the cell primarily accounted for the strong bactericidal role of Cu(I), which may eventually lead to enhanced oxidative stress towards cell membrane, DNA, and functional proteins. The developed platform in this study is promising to be integrated into current water treatment industry.


2006 ◽  
Vol 305 (2) ◽  
pp. 321-324 ◽  
Author(s):  
Cao Huiqun ◽  
Zhu Meifang ◽  
Li Yaogang

2011 ◽  
Vol 356-360 ◽  
pp. 277-282 ◽  
Author(s):  
Hsi Chi Yang ◽  
Jung Pin Wang ◽  
Chien Te Hsieh

This article reports a microwave-assisted route to synthesize nanosilver colloidal suspension and to deposit silver nanoparticles onto activated carbon fabrics (ACFs). The properties of the nanosilver suspension are characterized in terms of bacterial inactivation and growth inhibition. The metallic Ag nanocrystals with narrow size distribution are uniformly dispersed onto ACFs under the microwave irradiation of 1 min. Microwave irradiation is capable of heating up the reaction solution homogeneously, inducing uniform nucleation and rapid crystal growth to form the Ag crystallites. This work aims to elucidate how as-grown Ag nanoparticles affect the inactivation of Escherchia coli (E. coli) and how Ag-ACF surface inhibits the bacterial growth. The Ag colloidal suspension offers superior anti-bacterial ability against E. coli cells at a low concentration of 20 mg/L. Thus, the study has established a simple, efficient and effective process in the synthesis of both Ag colloidal suspension and Ag-ACF composite.


2019 ◽  
Vol 947 ◽  
pp. 13-20
Author(s):  
Jem Valerie D. Perez ◽  
Joy Vanessa D. Perez ◽  
Raniv D. Rojo ◽  
Maria Lourdes P. Dalida ◽  
Debora F. Rodrigues

Bacterial proliferation and biofilm formation has emerged as a significant concern in the long-term use of industrial apparatus. This study describes the antimicrobial properties of a novel chitosan-polyethyleneimine-graphene oxide (CS-PEI-GO) nanocomposite against E. coli. The nanocomposite is a stable material with minimal dispersibility in storage water after more than 7 days. The antimicrobial activity is contact-time-dependent, with direct contact (92% bacterial inactivation after 3h exposure) having superior results compared with dynamic contact (~50% inactivation after 3h exposure). In addition, the incorporation of GO also translated to enhanced production of ROS—oxidation of GSH was higher in CS-PEI-GO (31.78%) as compared to CS-PEI alone (5.69%). This may be attributed to previously proposed mechanisms of mechanical membrane damage and reactive oxygen species production that may be more pronounced with prolonged contact. This may be due to the positively charged chitosan and the negatively charged cell membrane facilitating the coating of cells that could allow the oxygen-containing functional groups of GO to induce oxidative stress and lead to cell death.


2016 ◽  
Vol 14 (4) ◽  
pp. 609-616 ◽  
Author(s):  
Zohreh Rashmei ◽  
Hamid Bornasi ◽  
Mahmood Ghoranneviss

In this paper, the disinfection of water is investigated using plasma spark treatment and the results are compared with conventional techniques. Inactivation of the Enterococcus faecalis and Escherichia coli bacteria is considered in the treatment process of water by the plasma spark. For this purpose, many physical and chemical parameters of water are measured and the obtained results demonstrate a reduction of 8-log in colony forming units of E. coli and E. faecalis at 15 minutes and 12 minutes, respectively. The results of this research show that no ozone is produced during the plasma spark treatment. Moreover, inactivation of a large number of bacteria without any change of pH shows that pH is not the cause of the bacterial inactivation. It is concluded that the main causes of the inactivation of bacteria in the treated water are H2O2 molecules and the electrical fields generated by plasma.


2003 ◽  
Vol 66 (9) ◽  
pp. 1637-1641 ◽  
Author(s):  
MARA C. L. NOGUEIRA ◽  
OMAR A. OYARZÁBAL ◽  
DAVID E. GOMBAS

The production of thermally concentrated fruit juices uses temperatures high enough to achieve at least a 5-log reduction of pathogenic bacteria that can occur in raw juice. However, the transportation and storage of concentrates at low temperatures prior to final packaging is a common practice in the juice industry and introduces a potential risk for postconcentration contamination with pathogenic bacteria. The present study was undertaken to evaluate the likelihood of Escherichia coli O157: H7, Listeria monocytogenes and Salmonella surviving in cranberry, lemon, and lime juice concentrates at or above temperatures commonly used for transportation or storage of these concentrates. This study demonstrates that cranberry, lemon, and lime juice concentrates possess intrinsic antimicrobial properties that will eliminate these bacterial pathogens in the event of postconcentration recontamination. Bacterial inactivation was demonstrated under all conditions; at least 5-log Salmonella inactivation was consistently demonstrated at −23°C (−10°F), at least 5-log E. coli O157:H7 inactivation was consistently demonstrated at −11°C (12°F), and at least 5-log L. monocytogenes inactivation was consistently demonstrated at 0°C (32°F).


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