scholarly journals An integrated homogeneous SPARCL™ immunoassay for rapid biomarker detection on a chip

2019 ◽  
Vol 11 (19) ◽  
pp. 2542-2550 ◽  
Author(s):  
Natalia Sandetskaya ◽  
Nicole Isserstedt-John ◽  
Andreas Kölsch ◽  
Sebastian Schattschneider ◽  
Dirk Kuhlmeier
Keyword(s):  
Quantitative Detection ◽  
Biomarker Detection ◽  
On Chip ◽  
Detection Of Biomarkers

A very simple and rapid homogeneous SPARCL™ immunoassay with on-chip preserved dry reagents enables sensitive quantitative detection of biomarkers.

scholarly journals A sample-to-answer electrochemical biosensor system for biomarker detection

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Kruthika Kikkeri ◽  
Dan Wu ◽  
Joel Voldman
Keyword(s):  
Electrochemical Biosensor ◽  
Blood Collection ◽  
Biomarker Detection ◽  
Plasma Separation ◽  
Collection Device ◽  
Detection Assay ◽  
Biosensor System ◽  
On Chip ◽  
Detection Of Biomarkers

We interfaced with a painless blood collection device and integrated on-chip blood-to-plasma separation with an electronic bead-based biomarker detection assay to enable true sample-to-answer detection of biomarkers.

scholarly journals Metal Nanoparticle and Quantum Dot Tags for Signal Amplification in Electrochemical Immunosensors for Biomarker Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 85
Author(s):  
Anton Popov ◽  
Benediktas Brasiunas ◽  
Asta Kausaite-Minkstimiene ◽  
Almira Ramanaviciene
Keyword(s):  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Metal Nanoparticle ◽  
Biomarker Detection ◽  
Analytical Instruments ◽  
Electrochemical Immunosensors ◽  
Highly Sensitive ◽  
Recent Developments ◽  
Electrochemical Signal ◽  
Detection Of Biomarkers

With the increasing importance of healthcare and clinical diagnosis, as well as the growing demand for highly sensitive analytical instruments, immunosensors have received considerable attention. In this review, electrochemical immunosensor signal amplification strategies using metal nanoparticles (MNPs) and quantum dots (Qdots) as tags are overviewed, focusing on recent developments in the ultrasensitive detection of biomarkers. MNPs and Qdots can be used separately or in combination with other nanostructures, while performing the function of nanocarriers, electroactive labels, or catalysts. Thus, different functions of MNPs and Qdots as well as recent advances in electrochemical signal amplification are discussed. Additionally, the methods most often used for antibody immobilization on nanoparticles, immunoassay formats, and electrochemical methods for indirect biomarker detection are overviewed.

scholarly journals Recent Advances in Portable Biosensors for Biomarker Detection in Body Fluids

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 127 ◽  
Author(s):  
Brian Senf ◽  
Woon-Hong Yeo ◽  
Jong-Hoon Kim
Keyword(s):  
Minimally Invasive ◽  
Clinical Intervention ◽  
Body Fluids ◽  
Disease Spread ◽  
Biomarker Detection ◽  
Sample Collection ◽  
Less Invasive ◽  
Recent Advances ◽  
Detection Of Biomarkers

A recent development in portable biosensors allows rapid, accurate, and on-site detection of biomarkers, which helps to prevent disease spread by the control of sources. Less invasive sample collection is necessary to use portable biosensors in remote environments for accurate on-site diagnostics and testing. For non- or minimally invasive sampling, easily accessible body fluids, such as saliva, sweat, blood, or urine, have been utilized. It is also imperative to find accurate biomarkers to provide better clinical intervention and treatment at the onset of disease. At the same time, these reliable biomarkers can be utilized to monitor the progress of the disease. In this review, we summarize the most recent development of portable biosensors to detect various biomarkers accurately. In addition, we discuss ongoing issues and limitations of the existing systems and methods. Lastly, we present the key requirements of portable biosensors and discuss ideas for functional enhancements.

On-chip quantitative detection of pathogen genes by autonomous microfluidic PCR platform

2015 ◽  
Vol 74 ◽  
pp. 725-730 ◽  
Author(s):  
Hiroaki Tachibana ◽  
Masato Saito ◽  
Shogo Shibuya ◽  
Koji Tsuji ◽  
Nobuyuki Miyagawa ◽  
...  
Keyword(s):  
Quantitative Detection ◽  
On Chip

scholarly journals Programmable Paper-Based Microfluidic Devices for Biomarker Detections

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 516 ◽  
Author(s):  
Veasna Soum ◽  
Sooyong Park ◽  
Albertus Ivan Brilian ◽  
Oh-Sun Kwon ◽  
Kwanwoo Shin
Keyword(s):  
Point Of Care ◽  
High Sensitivity ◽  
Microfluidic Devices ◽  
Portable Devices ◽  
Biomarker Detection ◽  
Fluid Delivery ◽  
Wide Range ◽  
Paper Based Microfluidics ◽  
Digital Microfluidic ◽  
Detection Of Biomarkers

Recent advanced paper-based microfluidic devices provide an alternative technology for the detection of biomarkers by using affordable and portable devices for point-of-care testing (POCT). Programmable paper-based microfluidic devices enable a wide range of biomarker detection with high sensitivity and automation for single- and multi-step assays because they provide better control for manipulating fluid samples. In this review, we examine the advances in programmable microfluidics, i.e., paper-based continuous-flow microfluidic (p-CMF) devices and paper-based digital microfluidic (p-DMF) devices, for biomarker detection. First, we discuss the methods used to fabricate these two types of paper-based microfluidic devices and the strategies for programming fluid delivery and for droplet manipulation. Next, we discuss the use of these programmable paper-based devices for the single- and multi-step detection of biomarkers. Finally, we present the current limitations of paper-based microfluidics for biomarker detection and the outlook for their development.

An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers

Lab on a Chip ◽  
2017 ◽  
Vol 17 (13) ◽  
pp. 2225-2234 ◽  
Author(s):  
Binfeng Hu ◽  
Juanjuan Li ◽  
Lei Mou ◽  
Yong Liu ◽  
Jinqi Deng ◽  
...  
Keyword(s):  
Quantitative Detection ◽  
Serum Samples ◽  
Chemiluminescence Immunoassay ◽  
Detection Of Biomarkers

An automated and portable microfluidic chemiluminescence immunoassay is developed for quantitative detection of biomarkers in serum samples.

scholarly journals Smart Integrated Sensor for Multiple Detections of Glucose and L-Lactate Using On-Chip Electrochemical System

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Tomoyuki Yamazaki ◽  
Takaaki Ikeda ◽  
Byounghyun Lim ◽  
Koichi Okumura ◽  
Makoto Ishida ◽  
...  
Keyword(s):  
Point Of Care ◽  
Reference Electrode ◽  
Electrochemical Sensing ◽  
Quantitative Detection ◽  
Enzymatic Reactions ◽  
Single Chip ◽  
Lactate Oxidase ◽  
Integrated Sensor ◽  
System L ◽  
On Chip

Multiple sensor electrodes, a supplementary electrode, a reference electrode, and signal-processing circuits were integrated on a single chip to develop a chip-shaped electrochemical sensing system. L-lactate and glucose were measured using on-chip working electrodes modified by polyion complex to immobilize lactate oxidase and glucose oxidase, respectively. Cyclic voltammetry measurements were conducted using an on-chip potentiostat. Selective and quantitative detection of glucose and L-lactate and the interference behavior were studied. Hydrogen peroxide generated by enzymatic reactions was detected by an increase in anodic oxidation current. Reaction currents at +0.7 V versus Ag/AgCl were used to obtain calibration plots. The measured dynamic ranges for L-lactate and glucose were 0.2–1.0 mM and 2.0–8.0 mM, respectively. The sensitivities were 65 nA/mM and 15 nA/mM, respectively, using a working electrode of 0.5 mm2. The 3σdetection limit was 0.19 mM and 1.1 mM, respectively. We have achieved multiple biomaterial detections on a circuit-equipped single chip. This integrated electrochemical sensor chip could be the best candidate for realizing point-of-care testing due to its portability and potential for mass production.

scholarly journals Biomarker detection technologies and future directions

The Analyst ◽  
2016 ◽  
Vol 141 (3) ◽  
pp. 740-755 ◽  
Author(s):  
Satish Balasaheb Nimse ◽  
Mukesh Digambar Sonawane ◽  
Keum-Soo Song ◽  
Taisun Kim
Keyword(s):  
Diagnosis And Treatment ◽  
Biomarker Detection ◽  
Multiplex Detection ◽  
Future Directions ◽  
Disease Prognosis ◽  
Detection Of Biomarkers ◽  
Detection Technologies

Advancement in technologies which can provide highly selective and sensitive multiplex detection of biomarkers is inevitable for accurate disease prognosis, diagnosis, and treatment follow-up.

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