Mechanisms for Lactobacillus rhamnosus treatment of intestinal infection by drug-resistant Escherichia coli

2020 ◽  
Vol 11 (5) ◽  
pp. 4428-4445 ◽  
Author(s):  
Na Li ◽  
Bing Pang ◽  
Junjun Li ◽  
Guanwen Liu ◽  
Xiaoguang Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Rhamnosus ◽  
Multiple Drug ◽  
Therapeutic Effects ◽  
Drug Resistant ◽  
Intestinal Infection ◽  
E Coli ◽  
Intestinal Integrity ◽  
Multiple Drug Resistant

Reducing the viability of pathogens may also play an important role for the therapeutic effects of Lactobacillus rhamnosus SHA113 against multiple-drug-resistant E. coli, as well as influencing on the intestinal integrity and functions of animals.

scholarly journals Antibacterial activities of aqueous and methanol extracts of Nigella sativa on some multiple drug resistant diarrheic bacterial agents

2020 ◽  
pp. 14-19
Author(s):  
Adedayo Emmanuel Ogunware ◽  
Hassan Zainab Adewunmi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Agents ◽  
Nigella Sativa ◽  
University Teaching ◽  
Multiple Drug ◽  
Significant Activity ◽  
Drug Resistant ◽  
E Coli ◽  
Methanolic Extracts ◽  
Multiple Drug Resistant

Combinations of various antimicrobial agents have been introduced as an extra successful strategy to combat multiple drug resistant infections. This study was undertaken to evaluate the effects of Nigella sativa seeds on several multi-drug resistant diarrheic bacterial agents. 30 Stool samples were collected from Lagos State University Teaching Hospital (LASUTH), in Nigeria and standard biochemical tests were performed to confirm the diarrheic isolates. Then, antimicrobial susceptibility testing was done on the organisms, followed by screening the effectiveness of Nigella sativa seed extracts on the bacterial agents obtained from the samples. 16 samples tested positive for diarrheic agents Escherichia coli, Escherichia coli 0157H:7 and Klebsiella pneumoniae. The methanolic extracts of Nigella sativa showed the highest zone of inhibition of 12mm for K. pneumoniae at a concentration of 300mg/dl ,10mm for E. coli 0157H:7 at a concentration of 200mg/dl, and 10mm for E. coli at a concentration of 200mg/dl, respectively. The methanolic extracts showed much stronger activity than the aqueous extracts of Nigella sativa which did not show significant activity towards the diarrheic agents isolated.

In vitro evaluation of antibacterial potential of Andrographis paniculata against resistant bacterial pathogens methicillin resistant Staphylococcus aureus (MRSA) and multiple drug resistant Escherichia coli (MDR E. coli)

2016 ◽  
Vol 5 (03) ◽  
pp. 4879
Author(s):  
Srikala Ganapathy* ◽  
Karpagam S.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ethanolic Extract ◽  
Multiple Drug ◽  
Drug Resistant ◽  
E Coli ◽  
Multiple Drug Resistant

Rise of antibiotic resistant pathogenic bacteria namely Methicillin Resistant Staphylococcus aureus (MRSA) and Multiple drug resistant Escherichia coli (MDR E. coli results in reduced efficacy of currently used antibacterial agents. Medicinal plants serve as potential targets for biologically effective antibacterial agents. The present study determined the phytochemical and invitro antibacterial activity of ethanol, chloroform, hexane and water extracts of whole plant of Andrographis paniculata against MRSA and MDR Escherichia coli. Zone of inhibition diameters were measured. Compared to all the extracts, ethanolic extract showed highest activity. The antibacterial activity was absent in hexane and water extracts. Chloroform extracts showed moderately good activity. The antibacterial compounds found in ethanolic extract were flavanoids, saponins and alkaloids.

scholarly journals A novel report of colistin-resistant Escherichia coli carrying mcr-1 gene from animal and human feacal samples in Nigeria

2018 ◽  
Vol 1 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Olugbenga A. Olowe ◽  
Rita A. Olowe ◽  
Adeolu S. Oluremi ◽  
Olusolabomi J. Adefioye
Keyword(s):  
Escherichia Coli ◽  
Animal Husbandry ◽  
Multiple Drug ◽  
Colistin Resistance ◽  
Pcr Assay ◽  
Southwestern Nigeria ◽  
E Coli ◽  
Multiple Drug Resistant ◽  
Microbiological Methods ◽  
Faecal Samples

Background: The mobilized colistin resistance (m cr)-1 gene confers transferable colistin resistance. Reports of mcr-1-positive Escherichia coli (MCRPE) have attracted substantial attention. However, in Nigeria, there is no report of mcr-1 gene resistance. Since colistin is a last resort for multiple drug-resistant isolates, this study therefore report the prevalence of mcr-1 gene among E. coli isolated from human and animal sources. Methods: Out of a total of 280 samples collected from animal and hum an faecal samples from selected farms in Oyo and Osun States, Southwestern Nigeria between July 2015 and June 2016, 60 E. coli were identified using standard microbiological methods. The mcr-1 gene was detected in the isolates by conventional PCR assay. Results: The m cr-1 gene was low and not statistically significant (p≥0.05). It was detected in 5 (8.3%) of 60 E. coli isolates (4= animals; 1= human) Conclusion: This study is the first report of mcr -1 gene from E. coli from human and animal sources in Nigeria. This calls for urgent caution in the use of colistin in animal husbandry.

scholarly journals Virulence Characteristics and Genetic Affinities of Multiple Drug Resistant Uropathogenic Escherichia coli from a Semi Urban Locality in India

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e18063 ◽  
Author(s):  
Savita Jadhav ◽  
Arif Hussain ◽  
Savita Devi ◽  
Ashutosh Kumar ◽  
Sana Parveen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Multiple Drug Resistant

scholarly journals The plasmid system ofEscherichia colistrain UR12644: Identification and molecular characteristics of transposons involved in the generation of endogenous R-plasmids

1979 ◽  
Vol 34 (3) ◽  
pp. 287-301 ◽  
Author(s):  
F. Schöffl ◽  
A. Pühler
Keyword(s):  
Escherichia Coli ◽  
Molecular Characteristics ◽  
Multiple Drug ◽  
Restriction Map ◽  
Drug Resistant ◽  
Cleavage Sites ◽  
A Value ◽  
Multiple Drug Resistant ◽  
R Plasmids

SUMMARYTwo spontaneously formed R-plasmids (pFS401 and pFS402) originating from the multiple drug-resistantEscherichia colistrain UR12644 were found to carry transposable drug-resistance elements. Incompatibility between these two plasmids was used to select for transposition. An ampicillin transposon (Tn1781) residing on pFS401 and a tetracycline transposon (Tn1771) present on pFS402 were independently translocated to the endogenous RTF-plasmid pFS2. Molecular weight determinations of pFS2::Tn1781(Ap) and pFS2::Tn1771(Tc) revealed a value of 2·9 Mdal for Tn1781 and 7·1 Mdal for Tn1771. The arrangement of 3PstI and 1BamHI restriction endonuclease sites was found to be characteristic for the ampicillin transposon whereas the restriction map of Tn1771 features a nearly symmetrical location of 3EcoRI cleavage sites, two of them close to the termini and one in the middle of the transposon. A model is presented suggesting the existence of repetitive DNA-segments at these positions which represent the structural preconditions for the genetic properties of Tn1771. The role of a cryptic plasmid involved in the generation of the endogenous R-plasmids pFS401 and pFS402 is discussed.

scholarly journals Isolation and Characterization of VceC Gain-of-Function Mutants That Can Function with the AcrAB Multiple-Drug-Resistant Efflux Pump of Escherichia coli

2006 ◽  
Vol 188 (11) ◽  
pp. 3757-3762 ◽  
Author(s):  
Govindsamy Vediyappan ◽  
Tatyana Borisova ◽  
Joe A. Fralick
Keyword(s):  
Escherichia Coli ◽  
Outer Membrane ◽  
Efflux Pump ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Membrane Component ◽  
Gain Of Function ◽  
Isolation And Characterization ◽  
Multiple Drug Resistant ◽  
Major Facilitator

ABSTRACT VceC is the outer membrane component of the major facilitator (MF) VceAB-VceC multiple-drug-resistant (MDR) efflux pump of Vibrio cholerae. TolC is the outer membrane component of the resistance-nodulation-division AcrAB-TolC efflux pump of Escherichia coli. Although these proteins share little amino acid sequence identity, their crystal structures can be readily superimposed upon one another. In this study, we have asked if TolC and VceC are interchangeable for the functioning of the AcrAB and VceAB pumps. We have found that TolC can replace VceC to form a functional VceAB-TolC MDR pump, but VceC cannot replace TolC to form a functional AcrAB-VceC pump. However, we have been able to isolate gain-of-function (gof) VceC mutants which can functionally interface with AcrAB. These mutations map to four different amino acids located at the periplasmic tip of VceC. Chemical cross-linkage experiments indicate that both wild-type and gof mutant VceC can physically interact with the AcrAB complex, suggesting that these gof mutations are not affecting the recruitment of VceC to the AcrAB complex but rather its ability to functionally interface with the AcrAB pump.

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